JURNAL PEMULIAAN TANAMAN HUTAN最新文献_第4页

PENGUJIAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA UNTUK MENGETAHUI KESTABILAN GENETIK KLON JATI (Tectona grandis)

JURNAL PEMULIAAN TANAMAN HUTAN Pub Date : 2018-12-15 DOI: 10.20886/jpth.2018.12.2.127-135

I. Nurtjahjaningsih, Toni Herawan, Reza Permatasari Rachma, Anto Rimbawanto

{"title":"PENGUJIAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA UNTUK MENGETAHUI KESTABILAN GENETIK KLON JATI (Tectona grandis)","authors":"I. Nurtjahjaningsih, Toni Herawan, Reza Permatasari Rachma, Anto Rimbawanto","doi":"10.20886/jpth.2018.12.2.127-135","DOIUrl":"https://doi.org/10.20886/jpth.2018.12.2.127-135","url":null,"abstract":"This study aimed to test RAPD markers to assess genetic stability of teak clones. Two experimental steps were carried out. First, nine RAPD markers were screened to verify the level of polymorphic loci; second, the polymorphic loci were applied to test genetic stability of clones. To test polymorphism levels of the primers, DNA was isolated from eight leaf samples that were collected from a seed orchard located at Watusipat, Gunung Kidul. To verify genetic stability of clones, DNA was isolated from leaf samples of 24 ramets of 3 clones after second sub-culturing. Results showed that amplification of 5 out of 9 RAPD primers were be consisten and produced 12 polymorphic loci. The number of polymorphic alleles per locus ranged between 1 and 3; the allele sizes were between 400 and 1,050 base pairs (bps). The percentage of polymorphic loci was 100%; it meant that overall loci have high polymorphism level. Based on these loci showed that the 24 ramets are clones; there was no somaclonal variation or high genetic stability. However, these loci need to be validated using more stable DNA markers.","PeriodicalId":239445,"journal":{"name":"JURNAL PEMULIAAN TANAMAN HUTAN","volume":"2016 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127508652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SELEKSI DAN PEROLEHAN GENETIK PADA UJI KETURUNAN NYAWAI (Ficus variegata Blume) DI BANTUL

JURNAL PEMULIAAN TANAMAN HUTAN Pub Date : 2018-12-15 DOI: 10.20886/jpth.2018.12.2.95-104

L. Haryjanto, Prastyono Prastyono, Y. Hadiyan

{"title":"SELEKSI DAN PEROLEHAN GENETIK PADA UJI KETURUNAN NYAWAI (Ficus variegata Blume) DI BANTUL","authors":"L. Haryjanto, Prastyono Prastyono, Y. Hadiyan","doi":"10.20886/jpth.2018.12.2.95-104","DOIUrl":"https://doi.org/10.20886/jpth.2018.12.2.95-104","url":null,"abstract":"Nyawai (Ficus variegata Blume) is a fast growing species which is promising for forest industrial plantation. Tree improvement of nyawai was then initiated through some progeny trials involving wide range of genetic base in Bantul. Study on initial growth reported that nyawai originated from Cilacap-Pangandaran showed higher genetic variation than those from other locations. However, further growth performance including the effect of selection in the progeny trial was not reported. This study was aimed to observe the growth and genetic parameter of nyawai in the progeny trial at advanced age. The genetic gain resulted from series of within plot selection was also estimated. The design of progeny trial was a randomized complete block with 19 families from Cilacap-Pangandaran, 4 non-contiguous tree-plot, 7 blocks at spacing of 5 m × 5 m. The observed traits were height, diameter at breast height and volume at four years of age. The results of study showed that survival rate was high at 89%. The mean annual increment for height, diameter and volume were 1.52 m/yr; 2.35 cm/yr and 8.65(×10-3) m3/yr, respectively. The proportion of variance to the total variance for family, plot and within plot ranged from 0.06% to 2.10%, 25.54% to 27.50% and 70.57% to 73.91%, respectively. In general, narrow-sense heritability for individual, family and within family were low. Genetic gain from within family selection that was practiced as within plot selection using selection ratio 25% were also low ranging from 0.19% to1.91% for all traits.","PeriodicalId":239445,"journal":{"name":"JURNAL PEMULIAAN TANAMAN HUTAN","volume":"176 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121854473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PENGARUH MIKORIZA ARBUSKULA DAN INANG Portulaca sp. TERHADAP AKLIMATISASI PLANTLET CENDANA (Santalum album L.)

JURNAL PEMULIAAN TANAMAN HUTAN Pub Date : 2018-12-15 DOI: 10.20886/JPTH.2018.12.2.%P

T. Herawan, A. Putri

{"title":"PENGARUH MIKORIZA ARBUSKULA DAN INANG Portulaca sp. TERHADAP AKLIMATISASI PLANTLET CENDANA (Santalum album L.)","authors":"T. Herawan, A. Putri","doi":"10.20886/JPTH.2018.12.2.%P","DOIUrl":"https://doi.org/10.20886/JPTH.2018.12.2.%P","url":null,"abstract":"Cendana (Santalum album Linn.) produces high-value aromatic timbers that are needed in various industries. Cendana is proclaimed by IUCN including critically endangered tree species. Tissue culture for conservation and propagation of cendana is a promising technique to lessen endangered level and increase industrial raw material supply. The main problems of cendana tissue culture are stunted growth and high mortality of plantlet at acclimatization stage. The purpose of this research is to identify the effect of arbuscular mycorrhizal application in acclimatization of cendana tissue cultured plantlets with and without hostplant. A.III.4.14 clones from Genetic Conservation Plot in Gunung Kidul, Yogyakarta were used as rooting plantlet material; Acaulospora sp. and Gigaspora sp. were used as MA isolates, and Portulaca sp. was used as hostplant. Sand and compost were used as media acclimatization in nurseries. Fungicide solution was used for sterilizing plantlets. Cendana plantlets were planted together with the host and MA added according to the treatment. Incubation was carried out in a greenhouse for 16 weeks. Observation of seedlings height was carried out 4 weeks after the polybag cover opened. The results of this study showed that 5 grams and 7 grams of mycorrhizal treatment on a cendana plantlet planted without Portulaca sp. produced the lower mortality (8%) after 12 weeks incubation. The best average seedling height growth was in 5 grams MA with hostplant (5,17 cm ±1,21) after 16 weeks incubation in green house. The results of this study prove the importance of exogenous mycorrhizal enrichment and hostplant in the acclimatization of cendana tissue culture.","PeriodicalId":239445,"journal":{"name":"JURNAL PEMULIAAN TANAMAN HUTAN","volume":"73 2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114315384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

INVENTARISASI DAN IDENTIFIKASI PENYEBAB PENYAKIT PADA Acacia auriculiformis DI YOGYAKARTA 日惹Acacia auriculiformis的清单和病理识别

JURNAL PEMULIAAN TANAMAN HUTAN Pub Date : 2018-12-15 DOI: 10.20886/JPTH.2018.12.2.105-113

Nur Hidayati, Rina Laksmi Hendrati

{"title":"INVENTARISASI DAN IDENTIFIKASI PENYEBAB PENYAKIT PADA Acacia auriculiformis DI YOGYAKARTA","authors":"Nur Hidayati, Rina Laksmi Hendrati","doi":"10.20886/JPTH.2018.12.2.105-113","DOIUrl":"https://doi.org/10.20886/JPTH.2018.12.2.105-113","url":null,"abstract":"Acacia auriculiformis is a fast growing species mostly planted in marginal lands with less intensive in cultivation. Problems found on A. auriculiformis cultivation include disease attacks which then caused a significant economic reduction on the plantation. The aim of this study is to determine causes, intensity and severity of the diseases attacking A. auriculiformis plants. The research was conducted on two observation plots, in the nursery and in clonal bank area established in Yogyakarta. Genetic materials planted in the plots were collected from clonally propagated of trees selected in second generation progeny trial of A. auriculiformis established in Wonogiri, Central Java. Observations of disease signs and symptoms in the two plots were undertaken with 100% plants inventories in rainy and dry seasons. Postulate Koch was then performed on this study to identify the pathogens. The result showed that the powdery mildew caused by Oidium sp. is a dominant disease attacking 100% A. auriculiformis both in the nursery and on clonal bank areas, occurring not only during the rainy season but also during the dry season. There were also other diseases attacking A. auriculiformis namely black mildew caused by Meliola sp, phomopsis, leaf spot disease caused by Phomopsis sp. and root rot disease caused by Ganoderma steyaertanum.","PeriodicalId":239445,"journal":{"name":"JURNAL PEMULIAAN TANAMAN HUTAN","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131311089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

IDENTIFIKASI PENYEBAB PENYAKIT LODOH PADA SEMAI KALIANDRA (Calliandra callothyrsus) 卡丽亚德拉(Calliandra callothyrsus)中LODOH病因的识别

JURNAL PEMULIAAN TANAMAN HUTAN Pub Date : 2018-12-01 DOI: 10.20886/JPTH.2018.12.2.135-142

Nurlaila Hidayati

引用次数: 3

PENINGKATAN KUALITAS BIBIT NYAMPLUNG (Calophyllum inophyllum L) DAN MALAPARI (Pongamia pinnata L) DENGAN APLIKASI MIKORIZA DAN Trichoderma spp. 改进了菌根培养基和菌根培养基的应用。

JURNAL PEMULIAAN TANAMAN HUTAN Pub Date : 2018-06-29 DOI: 10.20886/JPTH.2018.12.1.77-87

Benyamin Dendang, Aditya Hani, Encep Rachman

{"title":"PENINGKATAN KUALITAS BIBIT NYAMPLUNG (Calophyllum inophyllum L) DAN MALAPARI (Pongamia pinnata L) DENGAN APLIKASI MIKORIZA DAN Trichoderma spp.","authors":"Benyamin Dendang, Aditya Hani, Encep Rachman","doi":"10.20886/JPTH.2018.12.1.77-87","DOIUrl":"https://doi.org/10.20886/JPTH.2018.12.1.77-87","url":null,"abstract":"Nyamplung and Malapari a potential bioenergy crops on marginal land to be developed on the coastal land. The constraints of the coastal land is the less of water and soil nutrient. Input technology is needed in order to suceed the crop planting such as using bio fertilizer. Mycorrhizae plays an essential role in helping plants to absorb phosphate and increase the resistance to drought. Trichoderma spp involves in producing beneficial hormones for metabolism and helps break down organic matter. Utilization of biofertilizer is still rarely used as treatment in bioenergy plant seedlings. This study aimed to determine the quality of nyamplung and malapari seedlings after mycorrhizal and Trichoderma spp application in the nursery. Experiments were arranged in a complete randomized block design (RCBD). The treatment of media: soil as control (T), soil+organic fertilizer with ratio of 3:1(TK), soil+organic fertilizer+mycorhizae 10 g (TKM), soil+organic fertilizer+Trichoderma spp 10 g (TKT), soil+organic fertilizer+mycorrhizal 10 g+Trichoderma 10 g (TC). Application was done when the seedlings reached 1 (one) month old. Biofertilizer was sown around seedling roots. Each treatment consisted of 50 samples and 3 replications so the total seedlings were 750 seedlings. The results showed that after 6 and 12 months treatment malapari with organic fertilizer + mikoriza + Tricoderma spp (TC) gained 67.5% taller and 427% larger on dry weight of leaf than the control, whereas in nyamplung seedlings, TC treatment resulted better growth with diameter 8% larger, root length 78.56% longer, and total dry weight 7.88% heavier compared to control (T).","PeriodicalId":239445,"journal":{"name":"JURNAL PEMULIAAN TANAMAN HUTAN","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124085589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

PERTUMBUHAN JABON (Anthocephalus cadamba Miq.) PADA LAHAN MARGINAL BERJENIS TANAH ULTISOL DI RIAU

JURNAL PEMULIAAN TANAMAN HUTAN Pub Date : 2018-06-29 DOI: 10.20886/JPTH.2018.12.1.51-63

A. Junaedi

引用次数: 3

EMBRIOGENESIS SOMATIK ROTAN TOHITI (Calamus inops Becc. ex Heyne)

JURNAL PEMULIAAN TANAMAN HUTAN Pub Date : 2018-06-29 DOI: 10.20886/JPTH.2018.12.1.41-50

Yelnititis Yelnititis

{"title":"EMBRIOGENESIS SOMATIK ROTAN TOHITI (Calamus inops Becc. ex Heyne)","authors":"Yelnititis Yelnititis","doi":"10.20886/JPTH.2018.12.1.41-50","DOIUrl":"https://doi.org/10.20886/JPTH.2018.12.1.41-50","url":null,"abstract":"The conventional propagation of tohiti rattan still faces problem because of infrequent fruiting season and limited seed production. Somatic embryogenesis is an alternative technique to solve the problem. The purpose of this experiment is to obtain the best growth regulator treatments for embryogenic callus and somatic embryo formation of tohiti rattan. Murashige and Skoog (MS) basal medium was used as growth medium. The experiment was conducted in three stages: seed germination, embriogenic callus induction and somatic embryo induction. MS medium without plant growth regulator was used for aseptic seed germination. MS medium supplemented with growth regulator of BA (Benzyl adenine) of 0.5 – 2.0 mg/l was used for embryogenic callus induction. MS medium supplemented with BA 1.0 mg/l in combination with hormone 2.4-D of 0.0 – 1.0 mg/l was used for somatic embryo induction. The seed germination percentage, visual performance on embryogenic callus and somatic embryo were observed. The results showed that the percentage of aseptically seed germination reached 90%. MS medium supplemented with 1.0 mg/l BA is the best media for embryogenic callus induction with friable, white and yellowish of callus which was observed after four months of induction culture. The BA of 1.0 mg/l in combination with 2.4-D of 1.0 mg/l provided the highest number of the formed somatic embryo.The performance of somatic embryos formation from this treatment was likely as zygotic embryo.","PeriodicalId":239445,"journal":{"name":"JURNAL PEMULIAAN TANAMAN HUTAN","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121225126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

INTERAKSI FAMILI × LOKASI PADA UJI KETURUNAN GENERASI KEDUA Eucalyptus pellita INTERAKSI FAMILI × LOKASI PADA UJI KETURUNAN GENERASI KEDUA 尤加利树

JURNAL PEMULIAAN TANAMAN HUTAN Pub Date : 2018-06-29 DOI: 10.20886/JPTH.2018.12.1.25-39

Fasis Mangkuwibowo, S. Indrioko, Arif Nirsatmanto

{"title":"INTERAKSI FAMILI × LOKASI PADA UJI KETURUNAN GENERASI KEDUA Eucalyptus pellita","authors":"Fasis Mangkuwibowo, S. Indrioko, Arif Nirsatmanto","doi":"10.20886/JPTH.2018.12.1.25-39","DOIUrl":"https://doi.org/10.20886/JPTH.2018.12.1.25-39","url":null,"abstract":"The interaction assessment of genotype and environment is necessary to find out an effective selection strategy in progeny trial, particularly in advanced generations. This study aims to observe the effect of family × site interaction in second generation progeny trial of Eucalyptus pellita planted at two locations: Wonogiri, Central Java and Pelaihari, South Kalimantan. The trials design were completely randomized block design (CRBD) with 49 families, 6-10 blocks, 5 tree-plot and 4 m × 1.5 m spacing. Data measurements included height, diameter at breast height, and stem volume at two and four years age. The study results showed that the families had significant differences (p<0.01) for all measured traits at two and four years age, but the family and site interaction was not statistically significant. Family heritability across the two sites were moderate to high for all traits with the type B genetic correlation ranged from 0.67 to 0.94. Heritability and genetic correlation tended to be higher as tree getting older. Less than 16% of families showed an interactive rank changes in the two locations based on multiple-traits selection index. In general, family selection by combining data from the two sites of progeny trial provided higher genetic gain than those by indirect selection, but it was varied to those direct selection on each site. Therefore, these results imply that, the strategy for family selection by combining data from the two sites of progeny trials should be practiced in appropriate combination and direction by considering the magnitude of genetic variation.","PeriodicalId":239445,"journal":{"name":"JURNAL PEMULIAAN TANAMAN HUTAN","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134589634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

SELEKSI DAN PEROLEHAN GENETIK PADA UJI KETURUNAN GENERASI KEDUA KAYUPUTIH (Melaleuca cajuputi subsp. cajuputi) DI GUNUNGKIDUL

JURNAL PEMULIAAN TANAMAN HUTAN Pub Date : 2018-06-29 DOI: 10.20886/jpth.2018.12.1.67-75

S. Sumardi, Noor Khomsah Kartikawati, Prastyono Prastyono, Anto Rimbawanto

{"title":"SELEKSI DAN PEROLEHAN GENETIK PADA UJI KETURUNAN GENERASI KEDUA KAYUPUTIH (Melaleuca cajuputi subsp. cajuputi) DI GUNUNGKIDUL","authors":"S. Sumardi, Noor Khomsah Kartikawati, Prastyono Prastyono, Anto Rimbawanto","doi":"10.20886/jpth.2018.12.1.67-75","DOIUrl":"https://doi.org/10.20886/jpth.2018.12.1.67-75","url":null,"abstract":"Breeding for cajuput in Indonesia has moved into advanced generation breeding cycle through establishing second-generation progeny trial. A series of selection would be practiced in the trial before converting into a seedling seed orchard to produce genetically improved seed. In this study, a series of selection, followed by prediction of genetic gain will be observed in the second-generation progeny trial of cajuput established at Gunungkidul. The trial was laid out in randomized complete block design (RCBD), 65 families, three tree-plot, four replications, and spacing of 3 × 1,5 meter. Measurement was conducted on height and diameter at 2 years age. In the trial, within-plot selection has been practiced phenotypicaly by retaining one of the best tree out of the three tree within each plot. Succesive family selection was then simulated from the result of within-plot selection. Results of study showed that estimates of heritabily for diameter were higher than that for height. Genetic and phenotypic correlation between the two traits was moderate at around 0.57. Within-plot selection practiced in the trial resulted positive selection differential for all measured traits. Diameter showed higher coefficient weight of selection (0.4280) than height (0.0406) which indicates that the practiced within-plot selection was more imposed for diameter than that for height. Genetic gain from within-plot selection calculated using selection index were 20.76% for diameter and 12.73% for height. Simulated family selection using the same coefficient weight as within-plot selection resulted lower genetic gain at around 12.26% and 7.52% for diameter and height, respectivelly.","PeriodicalId":239445,"journal":{"name":"JURNAL PEMULIAAN TANAMAN HUTAN","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133608208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2