PENGUJIAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA UNTUK MENGETAHUI KESTABILAN GENETIK KLON JATI (Tectona grandis)

JURNAL PEMULIAAN TANAMAN HUTAN Pub Date : 2018-12-15 DOI:10.20886/jpth.2018.12.2.127-135

I. Nurtjahjaningsih, Toni Herawan, Reza Permatasari Rachma, Anto Rimbawanto

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Abstract

This study aimed to test RAPD markers to assess genetic stability of teak clones. Two experimental steps were carried out. First, nine RAPD markers were screened to verify the level of polymorphic loci; second, the polymorphic loci were applied to test genetic stability of clones. To test polymorphism levels of the primers, DNA was isolated from eight leaf samples that were collected from a seed orchard located at Watusipat, Gunung Kidul. To verify genetic stability of clones, DNA was isolated from leaf samples of 24 ramets of 3 clones after second sub-culturing. Results showed that amplification of 5 out of 9 RAPD primers were be consisten and produced 12 polymorphic loci. The number of polymorphic alleles per locus ranged between 1 and 3; the allele sizes were between 400 and 1,050 base pairs (bps). The percentage of polymorphic loci was 100%; it meant that overall loci have high polymorphism level. Based on these loci showed that the 24 ramets are clones; there was no somaclonal variation or high genetic stability. However, these loci need to be validated using more stable DNA markers.

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本研究旨在利用RAPD标记评价柚木无性系的遗传稳定性。进行了两个实验步骤。首先，筛选9个RAPD标记来验证多态性位点的水平;其次，利用多态位点检测无性系的遗传稳定性。为了测试引物的多态性水平，从位于Gunung Kidul Watusipat的种子果园收集的8个叶片样本中分离出DNA。为了验证无性系的遗传稳定性，从3个无性系的24个分株的叶片样品中进行了二次传代培养后的DNA分离。结果表明，9个RAPD引物中有5个扩增一致，产生12个多态性位点。每个位点的多态性等位基因数在1 ~ 3之间;等位基因大小在400 ~ 1050碱基对之间。多态性位点百分率为100%;总体上具有较高的多态性水平。结果表明，这24个品种均为无性系;无体细胞无性系变异，遗传稳定性高。然而，这些基因座需要使用更稳定的DNA标记进行验证。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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JURNAL PEMULIAAN TANAMAN HUTAN

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