Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie最新文献_第3页

Detection of staphylococcus aureus protein A by passive hemagglutination test using microtiter plates. 微滴板被动血凝试验检测金黄色葡萄球菌蛋白A。

Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie Pub Date : 1981-03-01

G Carret, J P Flandrois, J Fougerat

引用次数: 0

Oxygen-dependent metronidazole-resistance of Clostridium histolyticum. 溶组织梭菌氧依赖型甲硝唑耐药性。

Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie Pub Date : 1981-03-01

M Füzi

引用次数: 0

[T-proteins of Streptococcus pyogenes. III. Communication: purification of T-proteins extracted with trypsin, pepsin and C-phage-associated lysin by means of immunochromatography (author's transl)]. 化脓性链球菌的t蛋白。3通讯:用免疫层析法纯化胰蛋白酶、胃蛋白酶和c -噬菌体相关溶酶提取的t蛋白[作者的transl]。

Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie Pub Date : 1981-03-01

K H Schmidt, W Köhler

引用次数: 0

Antigenic properties and susceptibility to antibiotics of strains of Staphylococcus aureus in the 94, 96 phage complex. 金黄色葡萄球菌94,96噬菌体复合体的抗原特性及对抗生素的敏感性。

Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie Pub Date : 1981-03-01

J Fleurette, Y Brun

引用次数: 0

[Influence of antibodies on the phagocytosis of Klebsiella pneumoniae by alveolar macrophages (author's transl)]. [抗体对肺泡巨噬细胞吞噬肺炎克雷伯菌的影响[作者译]。

Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie Pub Date : 1981-03-01

C Undeutsch, H Brunner

{"title":"[Influence of antibodies on the phagocytosis of Klebsiella pneumoniae by alveolar macrophages (author's transl)].","authors":"C Undeutsch, H Brunner","doi":"","DOIUrl":"","url":null,"abstract":"Phagocytosis of Klebsiella pneumoniae by alveolar macrophages of guinea pigs was investigated. Lavage of guinea pig lungs yielded a cell suspension of approximately 85% alveolar macrophages. The remaining cells were predominantly lymphocytes and a few polymorphonuclear leucocytes. After incubation for 5 hours at 37 degrees C the macrophages adhered to the glass surface whereas leucocytes could be washed away. This resulted in a pure culture of macrophages containing 95-98% living cells. Specific antibodies to phenol-water extracts of K. pneumoniae were induced in rabbits. These antisera increased the rate of uptake of the bacteria by macrophages at least 35-fold as compared to macrophages without added antiserum. Preimmunization sera of rabbits also increased the uptake of bacteria by macrophages but by far less than hyperimmune sera. A very sensitive Enzyme-Immuno-Assay (ELISA) for detection of antibodies to the phenol-water extracts of K. pneumoniae was developed. Low levels of antibodies were demonstrated in the pre-immunization sera of rabbits by ELISA but not by the tube agglutination test. These low antibody titers could explain the increased uptake of the bacteria by alveolar macrophages in the presence of preimmunization sera. The influence of complement components on phagocytosis in our sera was small and was therefore not studied in any detail.","PeriodicalId":23929,"journal":{"name":"Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"249 1","pages":"43-52"},"PeriodicalIF":0.0,"publicationDate":"1981-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18069478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Influence of antibiotics on the phagocytosis of Klebsiella pneumoniae by alveolar macrophages (author's transl)]. [抗生素对肺泡巨噬细胞吞噬肺炎克雷伯菌的影响[作者译]。

Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie Pub Date : 1981-03-01

C Undeutsch, H Brunner

{"title":"[Influence of antibiotics on the phagocytosis of Klebsiella pneumoniae by alveolar macrophages (author's transl)].","authors":"C Undeutsch, H Brunner","doi":"","DOIUrl":"","url":null,"abstract":"The effect of gentamicin and cephalothin on the phagocytosis of Klebsiella pneumoniae by alveolar macrophages of guinea pigs was tested. At their minimal bactericidal concentration (MBC) the antibiotics did not influence the uptake of bacteria by macrophages in the presence of various antibody titers. As expected, the number of surviving bacteria after intracellular ingestion decreased at MBC of the antibiotics. The uptake of bacteria was inhibited by very high concentrations of gentamicin and cephalothin only. The intracellular killing of the bacteria was already higher in the presence of relatively low antibiotic concentrations (one third of the minimal inhibitory concentration, MIC) as compared to the killing without antibiotics. Above this levels, even if they were considerably higher than the MIC, the antibiotics had no additional effect on the number of bacteria surviving after ingestion. These findings indicate that concentrations above one third of the MIC of gentamicin or cephalothin are not necessarily of advantage for the effect of macrophages on the bacteria, provided that sufficient levels of antibodies are also present.","PeriodicalId":23929,"journal":{"name":"Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"249 1","pages":"53-62"},"PeriodicalIF":0.0,"publicationDate":"1981-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18069479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Bacteriocin typing of Enterobacter cloacae strains (author's transl)]. [阴沟肠杆菌菌株细菌素分型(作者译)]。

Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie Pub Date : 1981-03-01

V Freitag, O Friedrich

{"title":"[Bacteriocin typing of Enterobacter cloacae strains (author's transl)].","authors":"V Freitag, O Friedrich","doi":"","DOIUrl":"","url":null,"abstract":"By means of bacteriocin typing epidemic studies were carried out with regard to 65 strains of Enterobacter cloacae, isolated from various specimens of 59 patients. Based on detailed preliminary investigations the presentation with bacteriocins in liquid cultures induced by Mitomycin C was found most preferable. Since constant and reproducible results are essential, standardization of culture media, incubation temperature, incubation time and inoculum size is required. By means of 13 bacteriocin-producing strains the isolates could be typed and categorized into 24 types according to their sensitivity to bacteriocins. A rather varied picture emanated from the distribution of the individual types with regard to the different medical fields so that cross-infection with a certain strain was negligible. Seeing that one bacteriocin type was found predominantly in the intensive care unit, it can be maintained that this strain originated from the ward itself. The importance of bacteriocin typing for the interpretation of certain up-to-date epidemic situations is obvious which typing has also been successfully employed with regard to investigations of Enterobacter cloacae infections. However, it is doubtful whether bacteriocin production and bacteriocin sensitivity sufficiently constant in order to obtain comparable results over a more extended period of time and in different areas.","PeriodicalId":23929,"journal":{"name":"Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"249 1","pages":"63-75"},"PeriodicalIF":0.0,"publicationDate":"1981-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18069480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Extracellular toxins of Pseudomonas aeruginosa. II. Effect of two proteases on human immunoglobulins IgG, IgA and secretory IgA (author's transl)]. 铜绿假单胞菌胞外毒素。2两种蛋白酶对人免疫球蛋白IgG、IgA及分泌IgA的影响[作者译]。

Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie Pub Date : 1981-03-01

G Döring, H J Obernesser, K Botzenhart

引用次数: 0

The murein of citrobacter O-serogroup Ci23 Vi+. 柠檬酸杆菌o型血清群ci23vi +小鼠。

Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie Pub Date : 1981-03-01

K B Jastrzemski, B Kwiatkowski

引用次数: 0

[Extracellular toxins of Pseudomonas aeruginosa. I. Purification and characterization of two exoproteases (author's transl)]. 铜绿假单胞菌胞外毒素。1 .两种外蛋白酶的纯化和表征[作者译]。

Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie Pub Date : 1981-03-01

H J Obernesser, G Döring, K Botzenhart

{"title":"[Extracellular toxins of Pseudomonas aeruginosa. I. Purification and characterization of two exoproteases (author's transl)].","authors":"H J Obernesser, G Döring, K Botzenhart","doi":"","DOIUrl":"","url":null,"abstract":"Alkaline protease (protease I) and a protease with elastase activity (protease II) were isolated from two different strains of Pseudomonas aeruginosa (PA). Proteolytic activity was measured during the early exponential phase of growth and was highest when cultures reached the stationary growth phase. The extracellular character of protease I and II was demonstrated by measuring the intra- and extracellular ATP-concentration. Purification was achieved by precipitation with 65% ammonium sulfate, precipitation with 70% acetone, gelfiltration on Sephadex G-100 and chromatography on DEAE-Sephacel. The purified proteases were characterized. The pH for optimal proteolytic activity of protease I was at pH 9--10, for protease II at pH 8--9. Both enzymes cleaved casein and gelatine, in addition protein II elastin. Enzymatic activity of protease II was inhibited by 10(-3) M EDTA at pH 8.1 to 82%. Inactivation of protease I was not achieved by 10(-2) M EDTA. Molecular weight of protease I was estimated at 57,000, molecular weight of protease II at 39,000. Both enzymes consist of one polypeptide chain. In isoelectric focusing the protease I was separated into two components with pH values of 8.5 and 8.7, while protease II had isoelectric points of pH 6.0 and 6.4. Further characterization of protease I was done with amino acid analysis. Protease I was fairly stable over a pH range of 6--9 at room temperature. The optimal temperature for proteolytic activity was 60 degrees C. The results are discussed in view of proteases of other PA-strains.","PeriodicalId":23929,"journal":{"name":"Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"249 1","pages":"76-88"},"PeriodicalIF":0.0,"publicationDate":"1981-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17843433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0