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[Conjugational plasmid transfer from A, B and H streptococci to N streptococci]. [从A、B、H型链球菌到N型链球菌的共轭质粒转移]。
Zeitschrift fur allgemeine Mikrobiologie Pub Date : 1984-01-01 DOI: 10.1002/jobm.3630241014
I Riemelt, W Kirchhübel, H Malke
{"title":"[Conjugational plasmid transfer from A, B and H streptococci to N streptococci].","authors":"I Riemelt,&nbsp;W Kirchhübel,&nbsp;H Malke","doi":"10.1002/jobm.3630241014","DOIUrl":"https://doi.org/10.1002/jobm.3630241014","url":null,"abstract":"<p><p>Plasmid-mediated resistance to erythromycin and chloramphenicol was successfully transferred from group A, B and H streptococci to group N streptococci by a process akin to conjugation. The results showed that plasmids from streptococcal groups other than N were able to replicate in lactic streptococci as well. The transfer experiments were carried out by using a membrane filter mating technique. Four of the five plasmids used (pSM15346, pSM10419, pIP501, and pEL1) were transferred at frequencies ranging from 10(-1) to 10(-8) transconjugants per donor colony-forming unit. The highest transfer frequencies were obtained when S. pyogenes strain 15346 (pSM15346) served as the donor strain. The identy of transconjugants was verified by testing for the presence of unselected markers of the recipient strains, and both transduction and transformation were ruled out as the mechanisms of transfer.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"24 10","pages":"719-23"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17499677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
[A statistical method for the determination of the yield correlation of antibiotic-producing microorganisms]. [一种测定产抗生素微生物产率相关性的统计方法]。
Zeitschrift fur allgemeine Mikrobiologie Pub Date : 1984-01-01 DOI: 10.1002/jobm.3630240707
M Horn, E J Bormann, M Harnisch
{"title":"[A statistical method for the determination of the yield correlation of antibiotic-producing microorganisms].","authors":"M Horn,&nbsp;E J Bormann,&nbsp;M Harnisch","doi":"10.1002/jobm.3630240707","DOIUrl":"https://doi.org/10.1002/jobm.3630240707","url":null,"abstract":"<p><p>The antibiotic yields of industrial selectants must be checked at several steps of cultivation. Here the question arises whether the activities at the different levels of cultivation are correlated. The common coefficient of correlation cannot be used because repeated determinations of the yield at one selectant result in different values. In order to have only one fixed value per selectant we define the mean value around which the observed values are varying. But these mean values cannot be observed. Thus, an adequate method of correlation similar to that in Guiard and Herrendörfer (1977) was used. The method is demonstrated in two examples: With selectants of Streptomyces noursei for streptothricin titers in 20 ml- and 20 l-cultures as well as with selectants of Penicillium chrysogenum for potency indices in surface cultures and penicillin titers in 50 ml submerged cultures, respectively. In both cases the coefficients of correlation were above 0.7.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"24 7","pages":"443-9"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17156712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Formation of extracellular alpha-amylase by Bacillus subtilis in relation to guanosine polyphosphates. 枯草芽孢杆菌胞外α -淀粉酶的形成与鸟苷多磷酸的关系。
Zeitschrift fur allgemeine Mikrobiologie Pub Date : 1984-01-01 DOI: 10.1002/jobm.3630240814
R Wambutt, D Riesenberg, M Krüger, M Schultze
{"title":"Formation of extracellular alpha-amylase by Bacillus subtilis in relation to guanosine polyphosphates.","authors":"R Wambutt,&nbsp;D Riesenberg,&nbsp;M Krüger,&nbsp;M Schultze","doi":"10.1002/jobm.3630240814","DOIUrl":"https://doi.org/10.1002/jobm.3630240814","url":null,"abstract":"<p><p>The kinetics of growth, extracellular alpha-amylase formation and pool sizes of guanosine polyphosphates (p)ppGpp and adenosine phosphates (ATP and AMP) were determined during discontinuous cultivation of Bacillus subtilis 44. The results indicate a positive involvement of (p)ppGpp in the regulation of the expression of the alpha-amylase gene.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"24 8","pages":"575-9"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17498372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Properties of Streptomyces phage SLE111. 链霉菌噬菌体SLE111的性质
Zeitschrift fur allgemeine Mikrobiologie Pub Date : 1984-01-01 DOI: 10.1002/jobm.3630240913
U Taubeneck, I Zimmermann, F Walter, S Klaus
{"title":"Properties of Streptomyces phage SLE111.","authors":"U Taubeneck,&nbsp;I Zimmermann,&nbsp;F Walter,&nbsp;S Klaus","doi":"10.1002/jobm.3630240913","DOIUrl":"https://doi.org/10.1002/jobm.3630240913","url":null,"abstract":"<p><p>Phage SLE111 infecting Streptomyces levoris 1331 was morphological different from most actinophages described and yielded very high titres (10(11) p.f.u/ml) after lytic growth. Typical morphological changes of infected hyphae were observed by phase contrast and electron microscopy of ultrathin sections. The double-stranded, linear DNA (44.2 +/- 1.3 kb) was characterized, according to electron microscopic analysis, by absence of cohesive ends, of terminal redundance, and circular permutation. A stem-loop structure containing inverted repeats of about 200 bp was identified by electron microscopy in two alternative positions in the SLE111 genome.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"24 9","pages":"637-46"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17165308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Gene expression after transformation of Streptomyces lividans protoplasts with plasmid pIJ2 DNA. 质粒pIJ2 DNA转化lividans链霉菌原生质体后的基因表达。
Zeitschrift fur allgemeine Mikrobiologie Pub Date : 1984-01-01 DOI: 10.1002/jobm.3630240308
D Noack, G Fiedler, R Geuther
{"title":"Gene expression after transformation of Streptomyces lividans protoplasts with plasmid pIJ2 DNA.","authors":"D Noack,&nbsp;G Fiedler,&nbsp;R Geuther","doi":"10.1002/jobm.3630240308","DOIUrl":"https://doi.org/10.1002/jobm.3630240308","url":null,"abstract":"<p><p>A new method has been developed for the selection of antibiotic-resistant clones after transformation of Streptomyces protoplasts with plasmid DNA. This method is based on establishing a spatial concentration gradient for the antibiotic, the resistance to which is encoded by the transforming plasmid. By this method, the resistance development of regenerating protoplasts can be followed. The results suggest that antibiotic resistance is inducible. In addition, we were able to show that resident plasmids incompatible with the incoming ones are eliminated when this direct selection principle is used. Moreover, this method, which may facilitate the application of gene technology in Streptomyces, works even though the transformation procedure gives variable results.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"24 3","pages":"161-6"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17389400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
[Protein biosynthesis following heat shock in Bacillus subtilis]. [枯草芽孢杆菌热休克后的蛋白质生物合成]。
Zeitschrift fur allgemeine Mikrobiologie Pub Date : 1984-01-01 DOI: 10.1002/jobm.3630240606
G Wachlin, M Hecker
{"title":"[Protein biosynthesis following heat shock in Bacillus subtilis].","authors":"G Wachlin,&nbsp;M Hecker","doi":"10.1002/jobm.3630240606","DOIUrl":"https://doi.org/10.1002/jobm.3630240606","url":null,"abstract":"<p><p>Heat-activated spores of Bacillus subtilis synthesize during the early outgrowth special proteins which are absent from vegetative cells (Hecker 1983). Some of these outgrowth-specific proteins may be synthesized in response to heat activation of spores. In vegetative cells of B. subtilis synthesis of several proteins is markedly induced when temperature is shifted up from 30 to 44 degrees C. None of these putative heat shock proteins is synthesized during early outgrowth of heat-activated spores at 30 degrees C.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"24 6","pages":"397-401"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17491989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Dynamic model of discontinuous and continuous phaseolotoxin production of Pseudomonas syringae pv. phaseolicola. 丁香假单胞菌间断和连续产相毒素的动态模型。phaseolicola。
Zeitschrift fur allgemeine Mikrobiologie Pub Date : 1984-01-01
R Guthke, J Nüske, R Schorcht, W Fritsche, W A Knorre
{"title":"Dynamic model of discontinuous and continuous phaseolotoxin production of Pseudomonas syringae pv. phaseolicola.","authors":"R Guthke,&nbsp;J Nüske,&nbsp;R Schorcht,&nbsp;W Fritsche,&nbsp;W A Knorre","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>From experimental data of kinetics of growth, glucose consumption and product formation of Pseudomonas syringae pv. phaseolicola the development and parameter estimation of a mathematical model is presented. The model describes the behaviour of both, batch and chemostat culture, as well as for different temperatures. The model is favoured for dynamic optimization studies. Maximal productivity is reached in the chemostat for a dilution rate which is only a little bit smaller than the wash out point.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"24 7","pages":"427-35"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17533855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of protein patterns of Streptomyces griseus spores from surface and submerged cultures. 灰色链霉菌表面培养和深层培养孢子蛋白质模式的比较。
Zeitschrift fur allgemeine Mikrobiologie Pub Date : 1984-01-01
G Valu, S Vitális, I Békési, G Szabó
{"title":"Comparison of protein patterns of Streptomyces griseus spores from surface and submerged cultures.","authors":"G Valu,&nbsp;S Vitális,&nbsp;I Békési,&nbsp;G Szabó","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Protein patterns of conidia produced by a Streptomyces griseus strain in submerged cultures and on solid media were compared. Cell-free extracts (30 000 X g supernatant) were prepared and analyzed on gradient SDS polyacrylamide gels. The protein patterns of both kinds of conidia were found to be practically identical, and they differed from protein patterns of the old vegetative hyphae in a characteristic way.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"24 10","pages":"703-8"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17499676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ATP levels in phosphate-deregulated and arsenate-resistant mutants of Streptomyces noursei. 诺氏链霉菌脱除磷酸盐和抗砷酸盐突变体的ATP水平。
Zeitschrift fur allgemeine Mikrobiologie Pub Date : 1984-01-01 DOI: 10.1002/jobm.3630240407
F Hänel, U Gräfe, W Friedrich, E J Bormann
{"title":"ATP levels in phosphate-deregulated and arsenate-resistant mutants of Streptomyces noursei.","authors":"F Hänel,&nbsp;U Gräfe,&nbsp;W Friedrich,&nbsp;E J Bormann","doi":"10.1002/jobm.3630240407","DOIUrl":"https://doi.org/10.1002/jobm.3630240407","url":null,"abstract":"<p><p>Mycelial levels of ATP and glucose-6-phosphate were investigated in mutants of streptothricin-producing S. noursei JA 3880b differing from the wild-type strain in antibiotic formation, in the control by inorganic phosphate of the secondary metabolism, and in the resistance to growth inhibition by toxic arsenate ions. As compared with the ancestral strain, mutants exhibited a lower content of ATP in the mycelium while addition of 0.1 M arsenate to growing cultures provoked only moderate changes in the level of this high-energy metabolite. The results suggest that there exists a correlation between growth resistance to arsenate and insensitivity to phosphate inhibition of the secondary metabolism, on the one hand, and the capacity to produce streptothricin-type antibiotics, on the other.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"24 4","pages":"239-45"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17391956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Influence of tryptophan and related compounds on ergot alkaloid formation in Claviceps purpurea (FR.) Tul. 色氨酸及其相关化合物对麦角生物碱形成的影响。
Zeitschrift fur allgemeine Mikrobiologie Pub Date : 1984-01-01 DOI: 10.1002/jobm.3630241002
D Erge, B Schumann, D Gröger
{"title":"Influence of tryptophan and related compounds on ergot alkaloid formation in Claviceps purpurea (FR.) Tul.","authors":"D Erge,&nbsp;B Schumann,&nbsp;D Gröger","doi":"10.1002/jobm.3630241002","DOIUrl":"https://doi.org/10.1002/jobm.3630241002","url":null,"abstract":"<p><p>L-Tryptophan did not exert any influence on peptide alkaloid formation in an ergotamine and in an ergosine-accumulating C. purpurea strain. A different picture was observed in a series of related C. purpurea strains. Tryptophan showed a slight stimulatory effect on the ergotoxine producer Pepty 695/S. A blocked mutant of it, designated as Pepty 695/ch which was able to accumulate secoclavines gave similar results. In a high-yielding elymoclavine strain Pepty 695/e, the progeny of the former one, tryptophan up to a concentration of 25 mM stimulated remarkably clavine biosynthesis. Furthermore, tryptophan could overcome the block of synthesis by inorganic phosphate. Increased specific activities of chanoclavine cyclase but not DMAT synthetase were observed in cultures of strain Pepty 695/e supplemented with tryptophan. 5-Methyltryptophan and bioisosteres of tryptophan were ineffective in alkaloid stimulation. These results are compared with those obtained with the grass ergot strain SD 58 and discussed with the relation to other induction phenomena.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"24 10","pages":"667-78"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17582464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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