{"title":"灰色链霉菌表面培养和深层培养孢子蛋白质模式的比较。","authors":"G Valu, S Vitális, I Békési, G Szabó","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Protein patterns of conidia produced by a Streptomyces griseus strain in submerged cultures and on solid media were compared. Cell-free extracts (30 000 X g supernatant) were prepared and analyzed on gradient SDS polyacrylamide gels. The protein patterns of both kinds of conidia were found to be practically identical, and they differed from protein patterns of the old vegetative hyphae in a characteristic way.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"24 10","pages":"703-8"},"PeriodicalIF":0.0000,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparison of protein patterns of Streptomyces griseus spores from surface and submerged cultures.\",\"authors\":\"G Valu, S Vitális, I Békési, G Szabó\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Protein patterns of conidia produced by a Streptomyces griseus strain in submerged cultures and on solid media were compared. Cell-free extracts (30 000 X g supernatant) were prepared and analyzed on gradient SDS polyacrylamide gels. The protein patterns of both kinds of conidia were found to be practically identical, and they differed from protein patterns of the old vegetative hyphae in a characteristic way.</p>\",\"PeriodicalId\":23874,\"journal\":{\"name\":\"Zeitschrift fur allgemeine Mikrobiologie\",\"volume\":\"24 10\",\"pages\":\"703-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1984-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zeitschrift fur allgemeine Mikrobiologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zeitschrift fur allgemeine Mikrobiologie","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
本文比较了一株灰色链霉菌在液体培养基和固体培养基上产生的分生孢子的蛋白质模式。制备无细胞提取物(30 000 X g上清)，并在梯度SDS聚丙烯酰胺凝胶上分析。发现两种分生孢子的蛋白质模式几乎相同，但它们与老的营养菌丝的蛋白质模式有特征差异。
Comparison of protein patterns of Streptomyces griseus spores from surface and submerged cultures.
Protein patterns of conidia produced by a Streptomyces griseus strain in submerged cultures and on solid media were compared. Cell-free extracts (30 000 X g supernatant) were prepared and analyzed on gradient SDS polyacrylamide gels. The protein patterns of both kinds of conidia were found to be practically identical, and they differed from protein patterns of the old vegetative hyphae in a characteristic way.
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