链霉菌噬菌体SLE111的性质

U Taubeneck, I Zimmermann, F Walter, S Klaus
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引用次数: 1

摘要

感染左旋链霉菌1331的噬菌体SLE111在形态上与大多数已知的放线体不同,在裂解生长后产生非常高的滴度(10(11)p.f.u/ml)。采用超微切片相衬和电镜观察感染菌丝的典型形态变化。根据电镜分析,双链线性DNA (44.2 +/- 1.3 kb)具有无内聚末端、末端冗余和环状排列的特征。通过电子显微镜在SLE111基因组的两个位置鉴定出含有约200 bp倒置重复序列的茎环结构。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Properties of Streptomyces phage SLE111.

Phage SLE111 infecting Streptomyces levoris 1331 was morphological different from most actinophages described and yielded very high titres (10(11) p.f.u/ml) after lytic growth. Typical morphological changes of infected hyphae were observed by phase contrast and electron microscopy of ultrathin sections. The double-stranded, linear DNA (44.2 +/- 1.3 kb) was characterized, according to electron microscopic analysis, by absence of cohesive ends, of terminal redundance, and circular permutation. A stem-loop structure containing inverted repeats of about 200 bp was identified by electron microscopy in two alternative positions in the SLE111 genome.

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