Xenobiotica最新文献_第6页

Meeting report of the 4th European biotransformation workshop. 第四届欧洲生物转化研讨会会议报告。

IF 1.3 4区医学

Xenobiotica Pub Date : 2024-05-01 Epub Date: 2024-01-17 DOI: 10.1080/00498254.2024.2302547

M Walles, A Pähler, E M Isin, Marie M Ahlqvist

引用次数: 0

mRNA expression levels of cytochrome P450 CYP1A2, CYP3A4, and CYP3A5 in the epidermis: a focus on individual differences among Japanese individuals. 表皮中细胞色素 P450 CYP1A2、CYP3A4 和 CYP3A5 的 mRNA 表达水平：关注日本人的个体差异。

IF 1.3 4区医学

Xenobiotica Pub Date : 2024-05-01 Epub Date: 2024-04-24 DOI: 10.1080/00498254.2024.2344664

Hiroko Makihara, Mika Maezawa, Kazusa Kaiga, Toshihiko Satake, Mayu Muto, Yui Tsunoda, Tsutomu Shimada, Tomoko Akase

{"title":"mRNA expression levels of cytochrome P450 CYP1A2, CYP3A4, and CYP3A5 in the epidermis: a focus on individual differences among Japanese individuals.","authors":"Hiroko Makihara, Mika Maezawa, Kazusa Kaiga, Toshihiko Satake, Mayu Muto, Yui Tsunoda, Tsutomu Shimada, Tomoko Akase","doi":"10.1080/00498254.2024.2344664","DOIUrl":"10.1080/00498254.2024.2344664","url":null,"abstract":"Various cytochrome P450 enzymes (CYPs) that contribute to drug metabolism are expressed in the skin. However, variation among individuals in CYP expression profiles is not well-understood.To investigate CYPs related to the metabolism of transdermal preparations in Japan, multiple skin tissue specimens of individuals of Japanese descent were prepared, and the mRNA expression levels of CYP1A2, CYP3A4, and CYP3A5 were measured. Associations between the expression patterns of these CYPs and body mass index (BMI) were also investigated.There were considerable individual differences in epidermal CYP1A2 mRNA expression levels, and CYP1A2 showed a weak positive correlation with CYP3A4 mRNA expression levels. In contrast to previous results for other organs, epidermal CYP3A4 mRNA expression levels showed a weak positive correlation with BMI.CYP3A4 in the epidermis may have been locally enhanced as a defence mechanism against xenobiotics in response to impaired barrier function. These differences in mRNA expression in the skin may affect the transdermal absorption of drugs, such as lidocaine and fentanyl, which are metabolised by multiple overlapping CYPs.Our study provides new insights into drug metabolism in the skin. These results are valuable for predicting drug effects and transdermal drug transfer rates in Japanese patients.","PeriodicalId":23812,"journal":{"name":"Xenobiotica","volume":" ","pages":"226-232"},"PeriodicalIF":1.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140864986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of cytochrome P450 induction in canine intestinal organoid models. 评估犬肠道类器官模型中的细胞色素 P450 诱导。

IF 1.8 4区医学

Xenobiotica Pub Date : 2024-05-01 Epub Date: 2024-03-13 DOI: 10.1080/00498254.2024.2326973

Itsuma Nagao, Meg Nakazawa, Takashi Goyama, Michael H Court, Yoko M Ambrosini

{"title":"Assessment of cytochrome P450 induction in canine intestinal organoid models.","authors":"Itsuma Nagao, Meg Nakazawa, Takashi Goyama, Michael H Court, Yoko M Ambrosini","doi":"10.1080/00498254.2024.2326973","DOIUrl":"10.1080/00498254.2024.2326973","url":null,"abstract":"Understanding cytochrome P450 (CYP) enzymes in the canine intestine is vital for predicting drug metabolism and developing safer oral medications. This study evaluates canine colonoids as a model to assess the expression and induction of essential intestinal CYP enzymes.Canine colonoids were cultured in expansion medium (EM) with Wnt-3A and in differentiation medium (DM) without Wnt-3A. We assessed the mRNA expression of CYP2B11, CYP2C21, CYP3A12, and CYP3A98 using qPCR and examined the effects of rifampicin and phenobarbital as inducers.Our findings show that DM significantly increased the mRNA expression of CYP3A98 and CYP2B11, but not CYP3A12, compared to EM. CYP2C21, not typically expressed in the intestine, remained unexpressed in colonoids. Rifampicin induced CYP3A98, aligning with pregnane x receptor (PXR) regulation, while phenobarbital did not, suggesting no constitutive androstane receptor (CAR) involvement. CYP2B11 did not respond to either inducer, suggesting alternative regulatory pathways in canine colonoids.This study is a pioneering effort to establish conditions for studying P450 expression in canine colonoids, confirming significant CYP3A98 expression in the canine intestine. It demonstrated colonoids can induce CYP activity post drug treatments. Further research is needed to enhance species-specific drug metabolism understanding and validate this model for broader applications.","PeriodicalId":23812,"journal":{"name":"Xenobiotica","volume":" ","pages":"217-225"},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11178462/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140029127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation, characterisation, pharmacokinetics and distribution of esculin microspheres administered via intravitreal injection into rabbit brain 通过玻璃体内注射给药兔脑的艾司西林微球的制备、特性、药代动力学和分布

IF 1.8 4区医学

Xenobiotica Pub Date : 2024-04-19 DOI: 10.1080/00498254.2024.2341402

Danqing Wu, Rui Luo, Yangnan Chen, Zhiyun Zheng, Shuangying Gui, Ning He

引用次数: 0

Developmental stage and infection status may affect drug distribution in the prostate of rats 发育阶段和感染状况可能影响药物在大鼠前列腺中的分布

IF 1.8 4区医学

Xenobiotica Pub Date : 2024-04-18 DOI: 10.1080/00498254.2024.2343892

Ziyang Xu, Lianzhan Sun, Chang Yin, Handa Wang, Xue Wang, Yunyun Yang, Zhuo Wang

引用次数: 0

Selection of a Suitable Animal Model to Evaluate Secretion of Drugs in the Human Milk: A Systematic Approach 选择合适的动物模型来评估母乳中的药物分泌：一种系统方法