R Montes de Oca Porto, D Martinez Brito, O Terrero Serrano, M T Correa Vidal
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引用次数: 0
Abstract
1. 17ɑ-methyl-19-nortestosterone (MNT), a steroid prohibited in sport, has been marketed since the 1990s, however their use is increasingly, mainly because it is contained in nutritional supplements. The study of the metabolism of the prohibited substances for athletes allows to identify new metabolites that could increase the veracity of the results. This work studied preliminary the in-vivo metabolism of MNT.
2. The study was conducted after a single oral administration dose using the simple quadrupole mass spectrometry coupled to gas chromatography and considering the steroids classic metabolic reactions.
3. Nine MNT metabolites were detected in urine. Mass spectra and fragmentation patterns were proposed for each metabolite. In addition to MNT, the detection of four metabolites corroborated what is described in the specialised literature. However, to our knowledge, four other metabolites have not been explicitly described. According to the time-course, three metabolites were still detected at 96-, 84- and 72-hours port-administration. These detection windows can increase by using other instrumentations such as a triple quadrupole mass spectrometer with multiple reaction monitoring acquisition mode.
期刊介绍：
Xenobiotica covers seven main areas, including:General Xenobiochemistry, including in vitro studies concerned with the metabolism, disposition and excretion of drugs, and other xenobiotics, as well as the structure, function and regulation of associated enzymesClinical Pharmacokinetics and Metabolism, covering the pharmacokinetics and absorption, distribution, metabolism and excretion of drugs and other xenobiotics in manAnimal Pharmacokinetics and Metabolism, covering the pharmacokinetics, and absorption, distribution, metabolism and excretion of drugs and other xenobiotics in animalsPharmacogenetics, defined as the identification and functional characterisation of polymorphic genes that encode xenobiotic metabolising enzymes and transporters that may result in altered enzymatic, cellular and clinical responses to xenobioticsMolecular Toxicology, concerning the mechanisms of toxicity and the study of toxicology of xenobiotics at the molecular levelXenobiotic Transporters, concerned with all aspects of the carrier proteins involved in the movement of xenobiotics into and out of cells, and their impact on pharmacokinetic behaviour in animals and manTopics in Xenobiochemistry, in the form of reviews and commentaries are primarily intended to be a critical analysis of the issue, wherein the author offers opinions on the relevance of data or of a particular experimental approach or methodology
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