Acta Biomaterialia最新文献

{"title":"Impact of pH and chloride content on the biodegradation of magnesium alloys for medical implants: An in vitro and phase-field study","authors":"Sasa Kovacevic ,&nbsp;Wahaaj Ali ,&nbsp;Tushar Kanti Mandal ,&nbsp;Emilio Martínez-Pañeda ,&nbsp;Javier LLorca","doi":"10.1016/j.actbio.2025.03.034","DOIUrl":"10.1016/j.actbio.2025.03.034","url":null,"abstract":"<div><div>The individual contributions of pH and chloride concentration to the corrosion kinetics of bioabsorbable magnesium (Mg) alloys remain unresolved despite their significant roles as driving factors in Mg corrosion. This study demonstrates and quantifies hitherto unknown separate effects of pH and chloride content on the corrosion of Mg alloys pertinent to biomedical implant applications. The experimental setup designed for this purpose enables the quantification of the dependence of corrosion on pH and chloride concentration. The <em>in vitro</em> tests conclusively demonstrate that variations in chloride concentration, relevant to biomedical applications, have a negligible effect on corrosion kinetics. The findings identify pH as a critical factor in the corrosion of bioabsorbable Mg alloys. A variationally consistent phase-field model is developed for assessing the degradation of Mg alloys in biological fluids. The model accurately predicts the corrosion performance of Mg alloys observed during the experiments, including their dependence on pH and chloride concentration. The capability of the framework to account for mechano-chemical effects during corrosion is demonstrated in practical orthopedic applications considering bioabsorbable Mg alloy implants for bone fracture fixation and porous scaffolds for bone tissue engineering. The strategy has the potential to assess the <em>in vitro</em> and <em>in vivo</em> service life of bioabsorbable Mg-based biomedical devices.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"198 ","pages":"Pages 546-565"},"PeriodicalIF":9.4,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143813013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “The loading of C-type natriuretic peptides improved hemocompatibility and vascular regeneration of electrospun poly(ε-caprolactone) grafts” [Acta Biomaterialia 2022, 151, 304-316]","authors":"Jing Li ,&nbsp;Na Zhuo ,&nbsp;Jingai Zhang ,&nbsp;Qiqi Sun ,&nbsp;Jianghua Si ,&nbsp;Kai Wang ,&nbsp;Dengke Zhi","doi":"10.1016/j.actbio.2025.03.038","DOIUrl":"10.1016/j.actbio.2025.03.038","url":null,"abstract":"","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"198 ","pages":"Pages 566-568"},"PeriodicalIF":9.4,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polypeptide nanoparticles obstruct glucose supply for NIR-II fluorescence-guided tumor starvation and enhanced mild photothermal therapy","authors":"Yating Wang ,&nbsp;Yixuan Xu ,&nbsp;Dejia Chen ,&nbsp;Xin Li ,&nbsp;Dalong Yin ,&nbsp;Lifeng Yan","doi":"10.1016/j.actbio.2025.03.048","DOIUrl":"10.1016/j.actbio.2025.03.048","url":null,"abstract":"<div><div>Photothermal therapy (PTT) utilizes localized hyperthermia above 50 °C generated by nanomaterials upon exposure to near-infrared (NIR) laser light for effective cancer cell eradication. Yet, in high-temperature PTT, tumor cells develop heat stress tolerance due to elevated heat shock protein (HSP) levels, diminishing therapeutic efficacy. Moreover, excessive heat can trigger inflammatory conditions and promote cancer metastasis. Targeting the glycolytic pathway in highly metabolically active tumor cells offers a promising strategy for inducing starvation therapy, capitalizing on their vigorous energy demands amidst rapid proliferation. Here, we synthesized a highly luminous NIR-II dye, FNF, followed by its encapsulation alongside myricetin (My) within amphiphilic polypeptide carriers through a self-assembly method. The resulting nanoparticles exhibited great NIR-II imaging capabilities and boasted a notable photothermal conversion efficiency of 55.58 %. Furthermore, My effectively impeded glucose transport facilitated by glucose transporter protein 1 (GLUT1), curtailing glucose supply to tumor cells. This interference disrupted mitochondrial energy production, resulting in decreased adenosine triphosphate (ATP) synthesis and subsequent downregulation of HSP70 expression. By leveraging this approach, which targeted HSP expression via GLUT1 inhibition, we enhance the efficacy of PTT while achieving a synergistic effect for mild photothermal therapy through starvation.</div></div><div><h3>Statement of significance</h3><div>High expression of heat shock proteins (HSPs) in cancer cells impairs the efficacy of photothermal therapy (PTT) and triggers inflammation or metastasis, among other effects. Rapid malignant proliferation of tumor cells results in high energy metabolism, so interfering with their glucose metabolism to inhibit the glycolytic process is a feasible route for tumor starvation therapy. Here, we employed an amphiphilic polypeptide encapsulated photosensitizer (FNF) and myricetin (My) to construct nanoparticles with both NIR-II imaging capability and high photothermal conversion efficiency (55.58 %). Among them, My blocked glucose transport mediated by glucose transporter protein 1 (GLUT1), reduced the glucose supply and ATP synthesis in cancer cells, and then down regulated HSP70 expression. Thus, this strategy achieves starvation synergistic mild photothermal therapy through metabolic disruption.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"198 ","pages":"Pages 377-388"},"PeriodicalIF":9.4,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A partially degradable composite consisting of Ti-Zr-Cu-Pd-Sn metallic glass and Fe-Mg alloy for orthopedic applications","authors":"Peng Du ,&nbsp;Kun Zuo ,&nbsp;Rongqiang Yan ,&nbsp;Kun Li ,&nbsp;Shilu Chen ,&nbsp;Bo Yuan ,&nbsp;Liang Zhang ,&nbsp;Guoqiang Xie","doi":"10.1016/j.actbio.2025.04.011","DOIUrl":"10.1016/j.actbio.2025.04.011","url":null,"abstract":"<div><div>Partially-degradable biomaterials refers to smart implants where biodegradable metals can gradually be replaced by newly growing bone or living tissues, and leave behind a porous inert metal skeleton that stably binds with the new bone tissue. In this research, a partially degradable composite was designed by integrating Ti-Zr-Cu-Pd-Sn metallic glass (MG) with designed Fe-Mg alloy using spark plasma sintering (SPS). The mechanical alloying technique successfully enabled the fusion of immiscible Fe and Mg, addressing the issues of Fe's slow degradation and Mg's rapid breakdown, while also minimizing potential fractures in the metal framework due to hydrogen gas evolution. The controlled degradation of Mg(Fe) promotes the formation of Ca-P compounds, enhancing the bioactivity of the Fe-Mg composite. This design endows the composite with plastic and ductile deformation under compression, providing a viable solution to the brittle fracture behaviour commonly associated with conventional bulk metallic glasses (BMGs). This advancement holds promise for aligning with the natural growth rate of human bone, further augmenting the bioactive properties and practical applications of the MG/Fe-Mg composite material.</div></div><div><h3>Statement of significance</h3><div>In this research, a partially degradable composite was designed by integrating Ti-Zr-Cu-Pd-Sn metallic glass (MG) with designed Fe-Mg alloy using SPS. The Fe-Mg alloy act as temporary space holders can gradually being replaced by newly formed bone, thus establishing a dynamic equilibrium between the biodegradation of the bio-metals and the inward growth of new bone. The degradation of Mg(Fe) promotes the formation of Ca-P compounds, enhancing the bioactivity of the composite. This design endows the composite with plastic deformation under compression, providing a viable solution to the brittle fracture behavior of conventional MGs. This advancement holds promise for aligning with the natural growth rate of human bone, further augmenting the practical applications of the MG/Fe-Mg composite.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"198 ","pages":"Pages 514-529"},"PeriodicalIF":9.4,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced biodegradability, osteogenesis, and angiogenesis of Zn-Li alloys through calcium plasma immersion ion implantation","authors":"Xinlin Li ,&nbsp;Qi Li ,&nbsp;Ji Tan ,&nbsp;Yameng Yu ,&nbsp;Wei Yuan ,&nbsp;Xuanyong Liu ,&nbsp;Yufeng Zheng ,&nbsp;Dandan Xia","doi":"10.1016/j.actbio.2025.04.010","DOIUrl":"10.1016/j.actbio.2025.04.010","url":null,"abstract":"<div><div>Biodegradable zinc alloys are a promising research focus for bone implants due to their excellent bioactivity and mechanical properties, but the slow biodegradation rate and poor osseointegration limit their clinical application. In this study, calcium (Ca) ions were implanted into the surface of Zn-0.1Li alloys to improve their biodegradable, osteogenic, and angiogenic properties via the plasma immersion ion implantation (PIII) technique. FE-SEM and AFM results have demonstrated that Ca ion implantation appropriately increased the surface micro-roughness of the Zn-0.1Li alloy, thereby providing a larger contact surface area for biodegradation and osseointegration. Analysis using AES, XRD, and XPS revealed that Ca existed in the form of CaO. Based on SRIM simulations and FIB/TEM cross-sectional analysis, the thickness of the Ca ion implantation layer is approximately 52.2 nm, with a peak implantation dose exceeding 3.5 × 10⁵ atoms/cm². Static and electrochemical corrosion tests demonstrated that Ca ion implantation significantly accelerated the overall degradation behaviour of the Zn-0.1Li alloy and the release of zinc ions. <em>In vitro</em> experiments using MC3T3-E1 cells and HUVECs showed that Ca ion implantation samples significantly increased the expression of osteogenic genes (<em>ALP, Runx2, OCN</em>, and <em>Col I</em>) and angiogenic genes (<em>VEGF, eNOS</em>, and <em>FGFR</em>), demonstrating superior osteoinductive and angiogenic properties. Finally, <em>in vivo</em> rat femoral implantation studies revealed that Ca ion implantation samples exhibited enhanced biodegradation and osseointegration. In conclusion, Ca ion implantation effectively improved various properties of the Zn-0.1Li alloy, broadening its potential applications in dental and orthopedic fields.</div></div><div><h3>Statement of significance</h3><div>Zn alloys are one of the most promising biodegradable metals and have become a research hotspot in dentistry and orthopedics. However, the slow biodegradation rate and poor osteoinductivity severely limit their clinical application. In this study, we implanted Ca ions into the surface of Zn-0.1Li alloys via PIII to modulate their properties. <em>In vitro</em> and <em>in vivo</em> studies have confirmed that Ca ion implantation can effectively improve the surface micromorphology, biodegradability, osteoinductivity and angiogenic properties of Zn-0.1Li alloys, making them more prospective for use in bone implants.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"198 ","pages":"Pages 497-513"},"PeriodicalIF":9.4,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contact-free characterization of nuclear mechanics using correlative Brillouin-Raman Micro-Spectroscopy in living cells","authors":"S. Kerdegari ,&nbsp;A.A. Passeri ,&nbsp;F. Morena ,&nbsp;G. Ciccone ,&nbsp;V. Bazzurro ,&nbsp;P. Canepa ,&nbsp;A. Lagomarsino ,&nbsp;S. Martino ,&nbsp;M. Mattarelli ,&nbsp;M. Vassalli ,&nbsp;A. Diaspro ,&nbsp;S. Caponi ,&nbsp;C. Canale","doi":"10.1016/j.actbio.2025.04.009","DOIUrl":"10.1016/j.actbio.2025.04.009","url":null,"abstract":"<div><div>Nuclear mechanics is a key parameter in regulating cell physiology, affecting chromatin accessibility and transcriptional regulation. The most established method to characterize the mechanics of biological materials at the sub-micrometer scale is based on atomic force microscopy (AFM). However, its contact-based nature limits the direct access to the nucleus. While some indirect methods have been proposed to measure nuclear mechanics in living cells, the readout is influenced by the overlaying cytoskeleton. For this reason, mechanical measurements on isolated nuclei are a common strategy to overcome this issue. However, the impact of the invasive preparation procedure on the measured properties is still unclear. To address this issue, we studied the mechanical properties of skin fibroblasts probing the nuclear region and of extracted nuclei using AFM and correlative Brillouin-Raman Micro-Spectroscopy (BRMS). The latter technique is a non-invasive method to image living systems in 3D, obtaining correlative information on the mechanical and chemical properties of the sample at specific points of interest. Using this approach, we demonstrated that extracted nuclei are significantly softer than intact ones. Moreover, we demonstrated the ability of BRMS to highlight mechanical features within living cells that were masked by the convolution with the cytosol in conventional AFM measurements. Overall, this study shows the importance of evaluating nuclear mechanics within the native environment where cellular homeostasis is preserved. We, therefore, suggest that BRMS offers a much deeper insight into nuclear mechanics compared to AFM, and it should be adopted as a reference tool to study nuclear mechanobiology.</div></div><div><h3>Statement of significance</h3><div>The cell nucleus, the largest eukaryotic organelle, is crucial for cellular function and genetic material storage. Its mechanical properties, often altered in disease, influence key processes like chromatin accessibility. Although atomic force microscopy (AFM) is a standard method for studying nuclear mechanics, isolating nuclear stiffness in living cells is challenging due to interference from the cytoskeleton and plasma membrane. We demonstrate that correlative Brillouin-Raman Micro-Spectroscopy (BRMS) enables non-contact, high-resolution measurement of nuclear mechanics, capturing sub-micron details. We compare the results from BRMS with that obtained on the same samples with AFM. BRMS enhances our understanding of nuclear stiffness in physiological conditions, offering valuable insights for researchers in the field of mechanobiology, biotechnology, medicine, and bioengineering.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"198 ","pages":"Pages 291-301"},"PeriodicalIF":9.4,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stretch activated molecule immobilization in disulfide linked double network hydrogels","authors":"Yuwan Huang ,&nbsp;Zihao Li ,&nbsp;Chavinya D. Ranaweera ,&nbsp;Pavithra B. Jayathilaka ,&nbsp;Md Shariful Islam ,&nbsp;Alaa Ajam ,&nbsp;Meredith N. Silberstein ,&nbsp;Kristopher A. Kilian ,&nbsp;Jamie J. Kruzic","doi":"10.1016/j.actbio.2025.04.013","DOIUrl":"10.1016/j.actbio.2025.04.013","url":null,"abstract":"<div><div>Inspired by how forces facilitate molecule immobilization in biological tissues to provide localized functionalization, tough hydrogel networks with stretch activated mechanochemistry are developed by utilizing disulfide bonds as dynamic covalent crosslinks. Specifically, disulfide linked polyethylene glycol hydrogels are reinforced with a second ionically bonded sodium alginate network to simultaneously achieve stretchability and mechanochemical functionalization. To demonstrate and quantify the mechanochemical response, thiols produced by disulfide bond rupture are sensed during stretching using a reaction activated fluorophore dissolved in the hydrating solution. By monitoring the increase in fluorescence intensity upon stretching, it is determined that disulfide bond breakage in the double network hydrogels becomes more activated in hydrogels with high stretchability under low stress. Such results provide guidance regarding how the molecular weights and mass fractions of the monomers must be chosen to design double network hydrogels that balance favorable mechanical properties and mechanochemical responsiveness. Finally, for the most mechanochemically active hydrogel, we demonstrate how the stretch-activated immobilization of a maleimide containing peptide can functionalize the gels to promote the growth of human fibroblasts. Results of this work are anticipated to encourage further research into the development of stretchable and multifunctionalizable hydrogels for biotechnology and biomedical applications.</div></div><div><h3>Statement of significance</h3><div>Inspired by the mechanochemical dynamics in biological tissues, this work demonstrates the development of hydrogel-based biomaterials that can achieve stretch activated functionalization by molecule immobilization in multiple distinct ways. Using disulfide linked polyethylene glycol hydrogels reinforced with a second alginate network, we have elucidated the structure-property relationships of our hydrogels by functionalizing them with fluorophore to ensure a robust combination of stretchability and mechanochemical responsiveness. We also have demonstrated the capability for using stretch activated immobilization of functional peptides to guide human fibroblasts activity. By demonstrating how hydrogel network properties impact both mechanical and functional performance, this work opens pathways for designing multifunctionalizable hydrogels that adapt to mechanical forces, potentially broadening the application of hydrogels in biotechnology and biomedical applications.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"198 ","pages":"Pages 174-187"},"PeriodicalIF":9.4,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The tubular cavity of tobacco mosaic virus shields mechanical stress and regulates disassembly","authors":"A. Díez-Martínez ,&nbsp;P. Ibáñez-Freire ,&nbsp;R. Delgado-Buscalioni ,&nbsp;D. Reguera ,&nbsp;A.M. Bittner ,&nbsp;P.J. de Pablo","doi":"10.1016/j.actbio.2025.04.012","DOIUrl":"10.1016/j.actbio.2025.04.012","url":null,"abstract":"<div><div>Here we probe Tobacco mosaic virus (TMV) particles immobilized on a solid surface under transversal mechanical stress. We use atomic force microscopy to implement punctual deformation with high force (∼nN) that induces immediate virus rupture (single indentation assay), and continuous cycles of low force (∼100 pN) that generate a gradual disassembly of the virus particle (mechanical fatigue assay). These experiments are interpreted with the help of TMV coarse-grained and finite elements simulations, which indicate that the tubular cavity screens the transmission of mechanical stress from the top to the bottom half of the virion structure. Likewise, mechanical fatigue experiments reveal how TMV disassembles following growing transversal rifts with different dynamics that depend on a combination of the applied force and the tubular geometry of the virus. Our results indicate how the cylindrical cavity of TMV cushions the lower half of the virus structure from mechanical stress and regulates mechanical disassembly.</div></div><div><h3>Statement of significance</h3><div>The inability of plant viruses like tobacco mosaic virus (TMV) to infect mammals makes them ideal for technological applications. While TMV is known for it's durability, it's unclear if this is due solely to its capsid proteins or its tubular structure. Using Atomic Force Microscopy, coarse-grained and finite elements models, we found that the tubular hole screens the transmission of mechanical stress from the top to the bottom half of the virion structure. This characteristic induces a stepwise disassembly process from intact to half virus, finishing in the virion disruption. Since the energies between proteins are comparable to those of other viruses, there is a protective effect of the tubular cavity that transcends the size down to the nanoscale.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"198 ","pages":"Pages 356-365"},"PeriodicalIF":9.4,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polypropylene surgical mesh induces lipid oxidation in a nonhuman primate model","authors":"Amanda M. Artsen MD, MSc ,&nbsp;Craig A. Mayr ,&nbsp;Kristina Weber ,&nbsp;Krystyna Rytel ,&nbsp;Pamela A. Moalli","doi":"10.1016/j.actbio.2025.04.003","DOIUrl":"10.1016/j.actbio.2025.04.003","url":null,"abstract":"<div><div>Polypropylene mesh is widely used in surgery to support weak connective tissue, but pain and exposure complications limit vaginal implantation for pelvic organ prolapse. The increased complication rate with vaginal implantation is incompletely understood. We sought to compare the host response to low vs high polypropylene mesh burden after vaginal or abdominal implantation in rhesus macaques. We hypothesized that in both sites an increased mesh burden would result in increased malondialdehyde (MDA; a marker of lipid oxidative damage), heightened macrophage response, and increased apoptosis. Gynemesh PS and Restorelle implanted on the anterior abdominal wall were compared to a nonhuman primate sacrocolpopexy vaginal implantation model with Restorelle, which was intentionally and successively deformed to produce low, high, and highest mesh burden groups. Abdominal Gynemesh showed more CD68+ macrophages than lower mesh burden vaginal groups but not the highest burden group. In abdominal mesh, apoptosis by terminal deoxynucleotidyl transferase dUTP nick end labeling assay was limited to areas immediately surrounding mesh, while in deformed vaginal mesh, increased apoptosis was seen in the subepithelium. Macrophages and apoptotic cells were correlated at both sites and MDA was correlated with abdominal macrophages and vaginal apoptotic indices. Regardless of implantation site, macrophages, apoptotic indices, and MDA levels were strongly correlated with mesh burden. These data indicate that mesh burden is a main driver in the abdominal and vaginal mesh innate immune response and suggest a possible pathway through which prolonged inflammation contributes to tissue damage in mesh complications, namely through the immune cell production of reactive oxygen species or through stress-shielding.</div></div><div><h3>Statement of significance</h3><div>When implanted on the vagina, polypropylene mesh is associated with a strong negative foreign body response that can result in mesh exposure into the vagina or other organs. The mechanistic pathway for mesh exposure is unknown. Here, we show that polypropylene mesh induced lipid oxidation, as measured by malondialdehyde, in both abdominal and vaginal mesh implants in a nonhuman primate model. Mesh burden was strongly correlated with macrophages, apoptotic indices, and MDA levels. Apoptosis in the subepithelium in deformed mesh samples may be a result of stress shielding or oxidative damage and may contribute to exposure complications. These data suggest a possible pathway through which prolonged inflammation surrounding a biomaterial implant results in tissue damage and implant exposure.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"198 ","pages":"Pages 207-218"},"PeriodicalIF":9.4,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the role of calcium in the osteogenic behavior of mesoporous bioactive glass nanoparticles","authors":"José C. García-Perdiguero ,&nbsp;Natividad Gómez-Cerezo ,&nbsp;Miguel Gisbert-Garzarán ,&nbsp;Miguel Manzano ,&nbsp;María Vallet-Regí","doi":"10.1016/j.actbio.2025.04.005","DOIUrl":"10.1016/j.actbio.2025.04.005","url":null,"abstract":"<div><div>The use of bioactive materials has emerged as a promising strategy to circumvent bone-related diseases. Because of their chemical composition, calcium-containing bioactive glasses, including mesoporous bioactive glass nanoparticles (nMBG), have long demonstrated their bone regeneration features. In this work, SiO₂-CaO nMBG were synthesized varying Si/Ca ratio from 10 % to 40 % to explore the role of Ca in the osteogenic properties of such materials. We have performed an in-depth physicochemical and biological evaluation of samples by TEM, FTIR, adsorption nitrogen and solid state NMR, revealing that increasing calcium weakens the silica network and consequently, the osteogenic properties. In addition, we have evaluated the protein corona in human serum, obtaining varying protein patterns depending on the Si/Ca ratio and the incubation time. The cellular studies have shown that only certain amounts of calcium up-regulate the osteogenic differentiation, although exceeding such concentrations does not provide improved effects. Finally, All Ca-containing samples promoted calcium phosphate mineralization in biological fluids, while those with higher Si/Ca ratios enhanced significantly hMSC and hOB mineralization. Calcium also modulated hMSC gene expression, with samples containing up to 20 % calcium up-regulating OC and RUNX2. Furthermore, nMBG exhibited immunomodulatory properties, inducing a shift toward the M2 reparative phenotype. Overall, this comprehensive study highlights the crucial role of calcium in osteogenic responses, demonstrating that calcium quantity alone does not surpass the importance of structural and compositional quality in nanosized MBG.</div></div><div><h3>Statement of Significance</h3><div>Bone-related diseases are becoming a major socioeconomic issue owing to the increased aging of our society. Therefore, bioactive materials based on silicon, calcium and phosphorus have been used for years due to the osteogenic properties of these elements. In the last few years, the preparation of these materials as nanoparticles has increased their range of applications. In this sense, the novelty of our work relies on the in-depth physicochemical and biological evaluation of those mesoporous bioactive glass nanoparticles based on silicon and calcium, which remained unexplored so far. Couple with the establishment of the range of atomic percentage of calcium with respect to silicon that up-regulate the osteogenic differentiation, although exceeding such concentrations does not provide improved effects.</div></div>","PeriodicalId":237,"journal":{"name":"Acta Biomaterialia","volume":"198 ","pages":"Pages 482-496"},"PeriodicalIF":9.4,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}