Zeitschrift für Pflanzenphysiologie最新文献_第3页

Uptake and Processing of in vitro Synthesized Mitochondrial Malate Dehydrogenase by Isolated Watermelon Mitochondria 西瓜线粒体体外合成苹果酸脱氢酶的吸收和加工

Zeitschrift für Pflanzenphysiologie Pub Date : 1984-05-01 DOI: 10.1016/S0044-328X(84)80059-8

Christine Gietl, Bertold Hock

引用次数: 2

Effects of Inhibitors of Protein Synthesis on Abscisic Acid Accumulation in Wheat 蛋白质合成抑制剂对小麦脱落酸积累的影响

Zeitschrift für Pflanzenphysiologie Pub Date : 1984-05-01 DOI: 10.1016/S0044-328X(84)80085-9

S. Quarrie, P. G. Lister

引用次数: 34

Enzyme Activities and Leaf Constituents in Barley Seedlings at Different Nutrient Levels 不同营养水平下大麦幼苗酶活性及叶片成分

Zeitschrift für Pflanzenphysiologie Pub Date : 1984-05-01 DOI: 10.1016/S0044-328X(84)80063-X

Hansjörg Fritsch, Johannes Jung

引用次数: 6

Lipolytic and Glyoxysomal Enzyme Activities in Cotyledons of Ripening and Germinating Sunflower (Helianthus annuus L.) Seeds 向日葵成熟期和萌发期子叶脂溶酶和Glyoxysomal酶活性研究种子

Zeitschrift für Pflanzenphysiologie Pub Date : 1984-05-01 DOI: 10.1016/S0044-328X(84)80060-4

Anton Fusseder , Roland R. Theimer

{"title":"Lipolytic and Glyoxysomal Enzyme Activities in Cotyledons of Ripening and Germinating Sunflower (Helianthus annuus L.) Seeds","authors":"Anton Fusseder , Roland R. Theimer","doi":"10.1016/S0044-328X(84)80060-4","DOIUrl":"10.1016/S0044-328X(84)80060-4","url":null,"abstract":"<div>In cotyledons of sunflower (Helianthus annuus L.) seeds glyoxysomal enzyme activities were shown to develop already during seed maturation. Glyoxysomes isolated from cotyledons 30 d after anthesis exhibited activities of monoacylglycerol lipase, hydroxyacyl-CoA dehydrogenase, catalase, and malate synthase, while apparent isocitrate lyase activity could be measured only in the supernatant fluid of the sucrose density gradients yet not in the crude homogenates. This indicates the presence of a probably unspecific inhibitor especially since glyoxysomes from cotyledons 40 d after anthesis showed isocitrate lyase activity although still no activity could be detected in the crude homogenates. After dehydration of the mature seeds a glyoxysomal fraction could not be isolated which presumably is due to damage of the organelles by the strong forces required for tissue disintegration, for low activities of all but one glyoxysomal enzyme activities measured were present in the crude homogenates. Comparison of glyoxysomes from maturing seeds to those from germinating seeds revealed, in contrast to earlier reports in the literature, that during seed ripening low but substantial activities of all key enzymes for glyoxysomes are present in the organelles. It is suggested that these glyoxysomes function as intermediate forms immediately after seed imbibition and start of germination until the pleomorphic population of germination related glyoxysomes is developed.</div>","PeriodicalId":23797,"journal":{"name":"Zeitschrift für Pflanzenphysiologie","volume":"114 5","pages":"Pages 403-411"},"PeriodicalIF":0.0,"publicationDate":"1984-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0044-328X(84)80060-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85535525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Planteose Synthesis in Seeds of Sesamum indicum L. 芝麻种子中植物糖的合成。

Zeitschrift für Pflanzenphysiologie Pub Date : 1984-05-01 DOI: 10.1016/S0044-328X(84)80068-9

Herbert Hopf, Maria Spanfelner, Otto Kandler

{"title":"Planteose Synthesis in Seeds of Sesamum indicum L.","authors":"Herbert Hopf, Maria Spanfelner, Otto Kandler","doi":"10.1016/S0044-328X(84)80068-9","DOIUrl":"10.1016/S0044-328X(84)80068-9","url":null,"abstract":"<div>An enzyme, UDP-galactose : sucrose 6fru-α-galactosyltransferase, which synthesizes planteose (6fru-α-galactosylsucrose), has been purified 55-fold from seeds of Sesamum indicum L. (Pedaliaceae) using ammonium sulfate and protamine sulfate precipitation. The partially purified enzyme fraction showed only very low α-galactosidase and β-fructosidase activity but significant UDP-glucose 4-epimerase, phosphodiesterase, phosphatase and sucrose synthase activity which interfere with planteose synthesis. UDP-glucose 4-epimerase activity could be inhibited by about 50% by the addition of 10 mmol/l Mn2+, whereas phosphodiesterase activity could not be reduced by the addition of ATP, NAD or UDP-glucose, potentially alternative substrates for UDP-galactose. The optimal activity of the planteose synthesizing enzyme was obtained at pH 6.2. The enzyme was stable for at least six months at -20°C. It did not need sulfhydryl reagents for full activity. The enzyme displayed a high specificity for UDP-galactose (Km 0.2–0.5 mmol/l), the galactosyl donor, and for sucrose (Km 3.6–6.0 mmol/l), the acceptor. 5′-UMP was inhibitory, while galactose 1-phosphate, glucose l-phosphate and UDP-glucose did not affect the enzyme activity.</div>","PeriodicalId":23797,"journal":{"name":"Zeitschrift für Pflanzenphysiologie","volume":"114 5","pages":"Pages 485-492"},"PeriodicalIF":0.0,"publicationDate":"1984-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0044-328X(84)80068-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89042489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Distribution of serotonin in Juglans regia seeds during ontogenetic development and germination 5 -羟色胺在核桃种子个体发育和萌发中的分布

Zeitschrift für Pflanzenphysiologie Pub Date : 1984-05-01 DOI: 10.1016/S0044-328X(84)80090-2

F. Lembeck, G. Skofitsch

引用次数: 8

Production of Antibody against Spinach Calmodulin and its Application to Radioimmunoassay for Plant Calmodulin 菠菜钙调素抗体的制备及其在植物钙调素放射免疫测定中的应用

Zeitschrift für Pflanzenphysiologie Pub Date : 1984-05-01 DOI: 10.1016/S0044-328X(84)80062-8

Shoshi Muto, Shigetoh Miyachi

引用次数: 44

Evidence of Plastid Control of Abscisic Acid Accumulation in Barley (Hordeum vulgare L.) 质体控制大麦脱落酸积累的证据

Zeitschrift für Pflanzenphysiologie Pub Date : 1984-05-01 DOI: 10.1016/S0044-328X(84)80084-7

S.A. Quarrie, P.G. Lister

{"title":"Evidence of Plastid Control of Abscisic Acid Accumulation in Barley (Hordeum vulgare L.)","authors":"S.A. Quarrie, P.G. Lister","doi":"10.1016/S0044-328X(84)80084-7","DOIUrl":"https://doi.org/10.1016/S0044-328X(84)80084-7","url":null,"abstract":"<div>Two treatments affecting the functioning of barley (Hordeum vulgare L.) plastids were used to study the localisation and control of ABA accumulation in response to partial dehydration. The X-ray-induced barley mutant albostrians was used to compare ABA accumulation in green, white and striped mutant leaves, and in green leaves of albostrians which had been treated with the herbicide norflurazon. NF inhibits carotenoid synthesis and in high light results in photo-bleaching of chlorophyll.ABA accumulation was severely inhibited in completely white mutant leaves, in white portions of striped leaves, and in NF-treated leaves which had been photo-bleached. These leaves also had much lower stomatal conductances than those of green leaves, and plastid ribosomes were completely absent. NF had no effect on ABA accumulation in leaves grown in the dark, in low light (where NF reduced carotenoid but not chlorophyll levels) and in high light prior to the onset of photo-bleaching. In all these treatments plastid ribosomes were still present. Although hardly any ABA accumulation took place in white leaves, the presence of low levels of ABA in these leaves was indicated by UV isomerisation of assay samples.It was concluded firstly, that protein synthesis on plastid ribosomes was necessary at some stage for drought stress to elicit a rise in ABA synthesis, and secondly, that enzymes for every step in the synthesis of ABA are encoded on nuclear DNA.</div>","PeriodicalId":23797,"journal":{"name":"Zeitschrift für Pflanzenphysiologie","volume":"114 4","pages":"Pages 295-308"},"PeriodicalIF":0.0,"publicationDate":"1984-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0044-328X(84)80084-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91705648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

Modification of Pyruvate Kinase Activity in Cold-sensitive and Cold-resistant Leaf Tissues 冷敏感和抗寒叶片组织丙酮酸激酶活性的修饰

Zeitschrift für Pflanzenphysiologie Pub Date : 1984-05-01 DOI: 10.1016/S0044-328X(84)80083-5

E.A. Sobczyk, Z. Rybka, A. Kacperska

{"title":"Modification of Pyruvate Kinase Activity in Cold-sensitive and Cold-resistant Leaf Tissues","authors":"E.A. Sobczyk, Z. Rybka, A. Kacperska","doi":"10.1016/S0044-328X(84)80083-5","DOIUrl":"https://doi.org/10.1016/S0044-328X(84)80083-5","url":null,"abstract":"<div>In winter rape (Brassica napus, L., var. oleifera, cv. Górczański) and cucumber (Cucumis sativus, L., var. Inspektowy Warszawski) plants we found two types of pyruvate kinase. These were distinguished by different pH, ion and substrate requirements, as well as by their response to such negative modulators as ATP and alanine. Their response to temperature change was also different: the Arrhenius plot for the cucumber enzyme was linear over the temperature range 30°C to 0°C whereas that for the winter rape enzyme showed a break in the slope of the plot at 8°C. The cucumber enzyme extracted from plants exposed to chilling temperature (3°C) showed a transient increase of activity during the first days of cold treatment. The activity markedly decreased only after a pronounced (80%) protein degradation had occurred. Cold (2°C) acclimation of winter rape plants resulted in lower sensitivity of the enzyme to ATP, loss of sensitivity to alanine and decreased sensitivity to temperature below 10°C. The results are discussed in terms of plant adaptation to different temperature conditions of the environment.</div>","PeriodicalId":23797,"journal":{"name":"Zeitschrift für Pflanzenphysiologie","volume":"114 4","pages":"Pages 285-293"},"PeriodicalIF":0.0,"publicationDate":"1984-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0044-328X(84)80083-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91778417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Inhibition by Metabolic Intermediates of Pyrophosphate : Fructose 6-Phosphate Phosphotransferase from Germinating Castor Bean Endosperm 蓖麻胚乳中焦磷酸盐代谢中间体对果糖6-磷酸转移酶的抑制作用

Zeitschrift für Pflanzenphysiologie Pub Date : 1984-05-01 DOI: 10.1016/S0044-328X(84)80064-1

Erich Kombrink , Nicholas J. Kruger

引用次数: 32