World Journal of Gastroenterology最新文献

{"title":"Ligation-assisted endoscopic submucosal resection following the unroofing technique for esophageal lesions.","authors":"Zhong-Xing Ning, Jia-Jia Xiao","doi":"10.3748/wjg.v31.i12.100998","DOIUrl":"10.3748/wjg.v31.i12.100998","url":null,"abstract":"<p><p>For the treatment method of esophageal subepithelial lesions originating from the muscularis propria, conventional endoscopic resection techniques are time-consuming and lack efficacy for small subepithelial lesions originating from the muscularis propria. Lu <i>et al</i> presented an exploration of the effectiveness and safety of ligation-assisted endoscopic submucosal resection, aiming to provide a minimally invasive method for treatment. We discussed and analyzed this study from the aspects of sample screening, clinical pathological characteristics, case-control analysis, and follow-up data.</p>","PeriodicalId":23778,"journal":{"name":"World Journal of Gastroenterology","volume":"31 12","pages":"100998"},"PeriodicalIF":4.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11962843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic resistance of <i>Helicobacter pylori</i> and related risk factors in Hunan Province: A multicenter study.","authors":"Jie Xie, Ming-Lin Zhang, Min Guo, Jian-Guo Li, Yuan Liu, Hong-Hui Chen, Fen Wang","doi":"10.3748/wjg.v31.i12.104835","DOIUrl":"10.3748/wjg.v31.i12.104835","url":null,"abstract":"<p><strong>Background: </strong>Antibiotic resistance is a key factor influencing the treatment outcomes of <i>Helicobacter pylori</i> (<i>H. pylori</i>) infection. The antibiotic resistance spectrum of <i>H. pylori</i> varies in different regions. We investigated the current status of antibiotic resistance of <i>H. pylori</i> in Hunan Province and analyzed the factors related to such resistance to provide strategies for the accurate clinical treatment of <i>H. pylori</i> infection.</p><p><strong>Aim: </strong>To understand the antibiotic resistance of <i>H. pylori</i> in Hunan Province and provide guidance for the clinical treatment of <i>H. pylori</i> infection.</p><p><strong>Methods: </strong>This study selected patients who underwent gastroscopy in five hospitals in Hunan Province from April 2022 to April 2023. The sensitivity of <i>H. pylori</i> to clarithromycin, levofloxacin, metronidazole, amoxicillin, furazolidone, and tetracycline was detected using the Agar dilution method.</p><p><strong>Results: </strong><i>H. pylori</i> strains from a total of 566 patients were isolated and identified. The resistance rates of <i>H. pylori</i> strains to clarithromycin, levofloxacin, metronidazole, amoxicillin, furazolidone, and tetracycline were 49.2%, 37.8%, 76.1%, 2.3%, 1.4%, and 0.7%, respectively. The resistance rates to clarithromycin, levofloxacin, and metronidazole were high in the four regions of Hunan Province, and the overall resistance rates in central Hunan Province were higher than those in other regions. The resistance rates of <i>H. pylori</i> strains to clarithromycin and levofloxacin were significantly different among the different age groups (<i>P</i> < 0.05), with the elderly group having a higher resistance rate than the young group. The resistance rate of <i>H. pylori</i> strains to clarithromycin was greater in patients with atrophic gastritis, and the resistance rate to levofloxacin was the lowest in patients with peptic ulcers.</p><p><strong>Conclusion: </strong>The resistance rate of <i>H. pylori</i> to amoxicillin, clarithromycin, and metronidazole is high in Hunan Province. Age, stomach disease, and <i>H. pylori</i> reinfection may affect the antibiotic resistance of <i>H. pylori</i>.</p>","PeriodicalId":23778,"journal":{"name":"World Journal of Gastroenterology","volume":"31 12","pages":"104835"},"PeriodicalIF":4.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11962852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increased keratin 80 expression predicts poor prognosis and promotes oxaliplatin resistance in gastric cancer.","authors":"Bo Yang, Hong-Xia Cao, Ya-Wei He, Ji-Jie Ouyang, Meng Lv, Yong-Xiang Li, Yi-Da Lu","doi":"10.3748/wjg.v31.i12.103991","DOIUrl":"10.3748/wjg.v31.i12.103991","url":null,"abstract":"<p><strong>Background: </strong>Keratin 80 (KRT80), a type I intermediate filament protein, is a member of the keratin family with specialized functions in epithelial tissues. While KRT80 has been implicated in both normal physiological processes and various diseases, its role in gastric cancer (GC), particularly its expression and prognostic significance, remains poorly understood. In this study, we investigated the role and underlying molecular mechanisms of KRT80 in oxaliplatin resistance in GC. Our analysis revealed that KRT80 is significantly upregulated in GC tissues and is associated with poor clinical prognosis. The role of KRT80 in GC cell proliferation was assessed through <i>in vitro</i> and <i>in vivo</i> assays.</p><p><strong>Aim: </strong>To explore the expression of KRT80 in GC and its impact on the prognosis of patients.</p><p><strong>Methods: </strong>KRT80 expression in GC tissues was analyzed using Western blotting, quantitative reverse transcription PCR, multiple immunofluorescence staining, and immunohistochemistry. Survival analysis was conducted using the Kaplan-Meier method with the log-rank test. The role of KRT80 in GC cell proliferation was assessed through <i>in vitro</i> and <i>in vivo</i> assays. Immunoprecipitation and mass spectrometry analyses identified elongation factor 1-alpha 1 (EEF1A1) as a binding protein of KRT80.</p><p><strong>Results: </strong>Integrating our experimental findings with multiple published studies, we found that increased KRT80 expression is associated with poor prognosis in GC and promotes resistance to oxaliplatin. Moreover, we have preliminarily verified the interaction between KRT80 and EEF1A1. Therefore, this study provides a novel perspective on overcoming oxaliplatin resistance in GC.</p><p><strong>Conclusion: </strong>Increased KRT80 expression predicts poor prognosis and promotes oxaliplatin resistance in GC, suggesting its potential as a novel prognostic biomarker.</p>","PeriodicalId":23778,"journal":{"name":"World Journal of Gastroenterology","volume":"31 12","pages":"103991"},"PeriodicalIF":4.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11962845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of gallbladder stones associated with occult pancreaticobiliary reflux using computed tomography.","authors":"Chen Qiu, Yu-Kai Xiang, Hai Hu, Xuan-Bo Da, Gang Li, Yue-Yi Zhang, Hong-Lei Zhang, Cheng Zhang, Yu-Long Yang","doi":"10.3748/wjg.v31.i12.100855","DOIUrl":"10.3748/wjg.v31.i12.100855","url":null,"abstract":"<p><strong>Background: </strong>Occult pancreaticobiliary reflux (OPBR) is characterized by the absence of congenital anomalies at the pancreaticobiliary junction yet leads to altered bile composition and an increased incidence of gallbladder stones.</p><p><strong>Aim: </strong>To explore the computed tomography (CT) imaging characteristics of gallbladder stones in patients diagnosed with OPBR.</p><p><strong>Methods: </strong>We analyzed 362 patients undergoing cholecystectomy (November 2020 to January 2022). Intraoperative bile samples were assayed for amylase (> 110 U/L indicated OPBR). CT features, including stone density and visibility, were compared between 54 OPBR and 308 controls. Stone attenuation (HU) was measured under standardized conditions (uCT-780, 120 kVp, 160 mAs). Logistic regression and receiver operating characteristic curve analysis identified independent OPBR predictors, forming a validated nomogram.</p><p><strong>Results: </strong>OPBR patients exhibited significantly higher rates of CT-invisible stones (35.2% <i>vs</i> 12.3%) and uniform stones (87% <i>vs</i> 73.1%) along with lower overall stone density (<i>P</i> = 0.01). Logistic regression identified stone visibility, uniformity, and density as independent predictors. A nomogram integrating these features with patient age achieved high diagnostic accuracy (area under the curve = 0.71).</p><p><strong>Conclusion: </strong>CT imaging distinctly identifies gallbladder stone density, indicating a heightened risk of OPBR in patients with uniform and CT-invisible stones. Such imaging is crucial for preoperative assessments to evaluate potential recurrent biliary pathologies post-cholecystectomy.</p>","PeriodicalId":23778,"journal":{"name":"World Journal of Gastroenterology","volume":"31 12","pages":"100855"},"PeriodicalIF":4.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11962842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facing the challenges of autoimmune pancreatitis diagnosis: The answer from artificial intelligence.","authors":"You-Han Zhang, Ai-Qiao Fang, Hui-Yun Zhu, Yi-Qi Du","doi":"10.3748/wjg.v31.i12.102950","DOIUrl":"10.3748/wjg.v31.i12.102950","url":null,"abstract":"<p><p>Current diagnosis of autoimmune pancreatitis (AIP) is challenging and often requires combining multiple dimensions. There is a need to explore new methods for diagnosing AIP. The development of artificial intelligence (AI) is evident, and it is believed to have potential in the clinical diagnosis of AIP. This article aims to list the current diagnostic difficulties of AIP, describe existing AI applications, and suggest directions for future AI usages in AIP diagnosis.</p>","PeriodicalId":23778,"journal":{"name":"World Journal of Gastroenterology","volume":"31 12","pages":"102950"},"PeriodicalIF":4.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11962844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insights into the mechanisms of modified <i>Pulsatilla</i> decoction in alleviating chemotherapy-induced intestinal mucositis.","authors":"Ajitha G Gopika, Naresh Sachdeva","doi":"10.3748/wjg.v31.i12.105162","DOIUrl":"10.3748/wjg.v31.i12.105162","url":null,"abstract":"<p><p>Chemotherapy-induced intestinal mucositis (IM) is a prevalent complication affecting up to 80% of cancer patients undergoing treatment. Current therapies focus on symptomatic relief rather than addressing the underlying mechanism. Recent advances in integrative medicine highlight the potential of traditional Chinese medicine formulations as alternatives or adjuncts to existing therapies. In this context, this editorial discusses the recent results of a study published by Qiu <i>et al</i>, which investigates the multifaceted potential of modified <i>Pulsatilla</i> decoction (PD), a formulation of PD with licorice (<i>Glycyrrhiza uralensis</i>) and Ejiao (<i>Colla corii asini</i>), on 5-fluorouracil-induced IM in mice to alleviate clinical symptoms including diarrhea, weight loss, and intestinal damage. A series of histological, biochemical, bioinformatic, and microbiological assays evaluated body weight, diarrhea scores, inflammatory cytokine profiles, oxidative stress modulation, and microbiota composition. The findings indicated a reduction in diarrhea and oxidative stress, as well as an improvement in body weight and intestinal histopathology. Furthermore, the modified PD suppressed the <i>TLR4</i>/<i>MyD88</i>/nuclear factor kappa-B inflammatory pathway and down-regulated key pro-inflammatory cytokines. Moreover, the study underscores the role of gut microbiota in IM pathogenesis. Modified PD treatment reshaped microbial diversity by promoting beneficial genera such as <i>Bacteroides acidifaciens</i> while suppressing pathogenic species like <i>Salmonella</i>. These findings suggest that the therapeutic effects of the modified PD extend beyond inflammation modulation to encompass microbiome reprogramming and mucosal barrier repair. Although the study provides significant insights, several limitations still prevail. The broader implications of modified PD in gastrointestinal disorders and integrative oncology need further exploration.</p>","PeriodicalId":23778,"journal":{"name":"World Journal of Gastroenterology","volume":"31 12","pages":"105162"},"PeriodicalIF":4.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11962847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perirenal fat area is a preoperative predictor of hypertension resolution after laparoscopic sleeve gastrectomy: Generalized additive models.","authors":"Yue Li, Kai-Yuan Zheng, Zeng-Lin Liu, Tian-Ming Yu, Wen-Jie Zhang, Ming-Wei Zhong, San-Yuan Hu","doi":"10.3748/wjg.v31.i12.104952","DOIUrl":"10.3748/wjg.v31.i12.104952","url":null,"abstract":"<p><strong>Background: </strong>Laparoscopic sleeve gastrectomy (LSG) can lead to complete resolution of hypertension in most patients with obesity within one year. However, the preoperative factors related to this resolution are still unclear.</p><p><strong>Aim: </strong>To clarify the impact of relevant factors, particularly perirenal fat, on postoperative hypertension resolution.</p><p><strong>Methods: </strong>In this retrospective single-center study, a total of 138 patients with obesity and hypertension were included, all of whom underwent LSG in the hospital and were followed up for one year. Multivariate logistic regression models were used to identify independent risk factors for postoperative hypertension resolution. Generalized additive models were employed to clarify the nonlinear relationships between these factors and hypertension resolution, and their predictive values were compared using fivefold cross-validation.</p><p><strong>Results: </strong>After LSG, 107 patients (77.5%) experienced hypertension resolution, while 31 patients (22.5%) did not achieve resolution. Both the preoperative perirenal fat area (PrFA) and perirenal fat thickness were independent risk factors for postoperative hypertension resolution (<i>P</i> < 0.001 <i>vs P</i> = 0.002). These factors are curvilinearly correlated with the hypertension resolution rate, but PrFA has a better predictive value than perirenal fat thickness dose (area under the curve = 0.846 <i>vs</i> 0.809). Compared with those with PrFA ≥ 18 cm², patients with PrFA < 18 cm² had a higher hypertension resolution rate [87% <i>vs</i> 68.1%; odds ratio (95% confidence interval) = 3.513 (1.367-9.902), <i>P</i> = 0.012].</p><p><strong>Conclusion: </strong>PrFA is a preoperative predictor of postoperative hypertension resolution. It is curvilinearly associated with the resolution rate, and patients with PrFA < 18 cm² have better hypertension resolution outcomes after LSG.</p>","PeriodicalId":23778,"journal":{"name":"World Journal of Gastroenterology","volume":"31 12","pages":"104952"},"PeriodicalIF":4.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11962850/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic yield of follow-up in patients undergoing surgery for non-metastatic colorectal cancer.","authors":"Noelia Sala-Miquel, José Carrasco-Muñoz, Soledad Bernabeu-Mira, Carolina Mangas-Sanjuan, Sandra Baile-Maxía, Lucía Madero-Velázquez, Victor Ausina, Ana Yuste, Lucía Gómez-González, Manuel Romero Simó, Pedro Zapater, Rodrigo Jover","doi":"10.3748/wjg.v31.i12.100155","DOIUrl":"10.3748/wjg.v31.i12.100155","url":null,"abstract":"<p><strong>Background: </strong>Evidence on adherence, diagnostic performance and impact on survival to intensive follow-up after surgery for colorectal cancer (CRC) is limited.</p><p><strong>Aim: </strong>To analyze the diagnostic performance of surveillance colonoscopy, computed tomography (CT), and tumor markers (TMs) in detecting CRC recurrence or metastasis during follow-up after CRC resection. Secondary objectives included degree of adherence to clinical practice guidelines surveillance recommendations and factors associated with adherence and all-cause and CRC mortality.</p><p><strong>Methods: </strong>The single-center retrospective cohort study including patients undergoing curative resection of stage I-III CRC during 2010-2015. Follow-up was performed using TMs every 6 months, yearly CT for 5 years, and colonoscopy at years 1 and 4. Demographic, primary tumor data, and results at follow-up were collected.</p><p><strong>Results: </strong>Of 574 included patients included, 153 had recurrences or metastases. Of this group, 136 (88.9%) were diagnosed by CT, 10 (6.5%) by CT and colonoscopy, and 7 (4.6%) by colonoscopy; only 67.8% showed TMs elevation. Adherence to follow-up recommendations was 68.8% for the first colonoscopy, 74% for the first CT scan, and 96.6% for the first blood test; these values declined over time. Younger age at diagnosis [odds ratio (OR) 0.93; 95%CI: 0.91-0.95], CRC stages I-II (OR 0.38; 95%CI: 0.24-0.61), and adherence to follow-up recommendations (OR 0.30; 95%CI: 0.20-0.46) were independently associated with lower risk for all-cause death at 5 years.</p><p><strong>Conclusion: </strong>CT scan had the highest diagnostic yield. Adherence to follow-up recommendations was low and decreased during follow-up. Younger age at diagnosis, stage, and follow-up adherence were associated with lower 5-year mortality.</p>","PeriodicalId":23778,"journal":{"name":"World Journal of Gastroenterology","volume":"31 12","pages":"100155"},"PeriodicalIF":4.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11962849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of hepatic pathology during azoxymethane-induced acute liver failure.","authors":"Shadikchhya Maya Bhattarai, Ashwin Jhawer, Gabriel Frampton, Eleonora Troyanovskaya, Sharon DeMorrow, Matthew McMillin","doi":"10.3748/wjg.v31.i12.103952","DOIUrl":"10.3748/wjg.v31.i12.103952","url":null,"abstract":"<p><strong>Background: </strong>Acute liver failure (ALF) is a loss of liver function due to a severe hepatic insult. Studies utilizing the azoxymethane (AOM) mouse model of ALF, which also generates hepatic encephalopathy, have primarily focused on development of neurological deficits. However, the molecular processes that generate liver damage have not been fully characterized. Therefore, a more comprehensive characterization of the hepatic consequences of AOM toxicity is needed to better understand this disease model.</p><p><strong>Aim: </strong>To identify molecular pathology contributing to hepatic injury during the progression of AOM-induced ALF.</p><p><strong>Methods: </strong>C57BL/6 mice were injected with AOM to produce ALF and hepatic encephalopathy. Tissue was collected at defined stages of neurological decline up to coma. Liver injury, CYP2E1 expression, oxidative stress, inflammation, apoptosis, necroptosis, and hepatocellular senescence were assessed.</p><p><strong>Results: </strong>Increased hepatic necrosis and exacerbated liver injury were observed after AOM injection as mice progressed towards coma. CYP2E1 expression decreased in AOM-treated mice as liver injury progressed. Malondialdehyde, myeloperoxidase and other measures of oxidative stress were significantly increased during AOM-induced ALF. Hepatic CCL2 and tumor necrosis factor α expression increased as AOM-induced liver injury progressed. Mixed lineage kinase domain-like protein phosphorylation was increased early during the progression of AOM-induced liver injury. Measures of apoptosis and cellular senescence all increased as the time course of AOM progressed.</p><p><strong>Conclusion: </strong>These data support that necrosis, oxidative stress, inflammation, apoptosis, and senescence were elevated in AOM-treated mice, with inflammation being the earliest significant change.</p>","PeriodicalId":23778,"journal":{"name":"World Journal of Gastroenterology","volume":"31 12","pages":"103952"},"PeriodicalIF":4.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11962848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decreased gene expression of interleukin 2 receptor subunit γ (CD132) in tissues of patients with Crohn's disease.","authors":"Juan Carlos Andreu-Ballester, Carolina Hurtado-Marcos, Carlos García-Ballesteros, Jaime Pérez-Griera, Fernando Izquierdo, Dolores Ollero, Ana Jiménez, Rafael Gil-Borrás, Antonio Llombart-Cussac, Francisca López-Chuliá, Carmen Cuéllar","doi":"10.3748/wjg.v31.i12.97120","DOIUrl":"10.3748/wjg.v31.i12.97120","url":null,"abstract":"<p><strong>Background: </strong>A deficiency of γδ T cells has been described in Crohn's disease (CD).</p><p><strong>Aim: </strong>To analyze the gene expression of interleukin 7 (IL-7) and its receptors in the tissues of patients with CD.</p><p><strong>Methods: </strong>We studied the peripheral blood of 80 patients with CD, comparing them with a group of 80 healthy subjects. The number and apoptosis of αβ and γδ T cells in peripheral blood and the proportion of αβ and γδ T cells in the intestinal tissues of patients with CD (<i>n</i> = 25) were studied. The gene and protein expression of IL-7, IL-2 receptor subunit γ [cluster of differentiation 132 (CD132)], receptor α (CD127), and caspase-3 in tissues was analyzed by quantitative PCR. Serum IL-7 levels were also analyzed.</p><p><strong>Results: </strong>In patients with CD, a decreased number of γδ T cells and an increase in the apoptosis of CD56+ αβ and γδ T cells in peripheral blood was observed (<i>P</i> < 0.0001 and <i>P</i> < 0.01) respectively, and there was an inverse correlation among T subsets and their apoptosis. In addition, IL-7 gene expression and IL-7 protein in the tissues of these patients were increased. The titers of caspase-3 in tissues were low <i>vs</i> control group (<i>P</i> > 0.01). The percentage of CD8+γδ T cells decreased in tissues (<i>P</i> < 0.01), and was directly related to IL-7 levels in peripheral blood. The expression of IL-2 receptor subunit γ (CD132) was greatly decreased in the tissues of patients with CD (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>There may be a cause-effect relationship between the lower gene expression of the IL-2 receptor subunit γ (CD132) in tissues of patients with CD and γδ T cells immunodeficiency.</p>","PeriodicalId":23778,"journal":{"name":"World Journal of Gastroenterology","volume":"31 12","pages":"97120"},"PeriodicalIF":4.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11962853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}