World journal of stem cells最新文献

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Extended protective effects of three dimensional cultured human mesenchymal stromal cells in a neuroinflammation model. 三维培养人间充质间质细胞在神经炎症模型中的扩展保护作用。
IF 3.6 3区 医学
World journal of stem cells Pub Date : 2025-01-26 DOI: 10.4252/wjsc.v17.i1.101485
Ok-Hyeon Kim, Hana Kang, Eun Seo Chang, Younghyun Lim, Young-Jin Seo, Hyun Jung Lee
{"title":"Extended protective effects of three dimensional cultured human mesenchymal stromal cells in a neuroinflammation model.","authors":"Ok-Hyeon Kim, Hana Kang, Eun Seo Chang, Younghyun Lim, Young-Jin Seo, Hyun Jung Lee","doi":"10.4252/wjsc.v17.i1.101485","DOIUrl":"10.4252/wjsc.v17.i1.101485","url":null,"abstract":"<p><strong>Background: </strong>Human mesenchymal stromal cells (MSCs) possess regenerative potential due to pluripotency and paracrine functions. However, their stemness and immunomodulatory capabilities are sub-optimal in conventional two-dimensional (2D) culture.</p><p><strong>Aim: </strong>To enhance the efficiency and therapeutic efficacy of MSCs, an <i>in vivo</i>-like 3D culture condition was applied.</p><p><strong>Methods: </strong>MSCs were cultured on polystyrene (2D) or in a gellan gum-based 3D system. <i>In vitro</i>, prostaglandin-endoperoxide synthase 2, indoleamine-2,3-dioxygenase, heme oxygenase 1, and prostaglandin E synthase gene expression was quantified by quantitative real-time polymerase chain reaction. MSCs were incubated with lipopolysaccharide (LPS)-treated mouse splenocytes, and prostaglandin E2 and tumor necrosis factor-alpha levels were measured by enzyme linked immunosorbent assay. <i>In vivo</i>, LPS was injected into the lateral ventricle of mouse brain, and MSCs were administered intravenously the next day. Animals were sacrificed and analyzed on days 2 and 6.</p><p><strong>Results: </strong>Gellan gum polymer-based 3D culture significantly increased expression of octamer-binding transcription factor 4 and Nanog homeobox stemness markers in human MSCs compared to 2D culture. This 3D environment also heightened expression of cyclooxygenase-2 and heme-oxygenase 1, enzymes known for immunomodulatory functions, including production of prostaglandins and heme degradation, respectively. MSCs in 3D culture secreted more prostaglandin E2 and effectively suppressed tumor necrosis factor-alpha release from LPS-stimulated splenocytes and surpassed the efficiency of MSCs cultured in 2D. In a murine neuroinflammation model, intravenous injection of 3D-cultured MSCs significantly reduced ionized calcium-binding adaptor molecule 1 and glial fibrillary acidic protein expression, mitigating chronic inflammation more effectively than 2D-cultured MSCs.</p><p><strong>Conclusion: </strong>The microenvironment established in 3D culture serves as an <i>in vivo</i> mimetic, enhancing the immunomodulatory function of MSCs. This suggests that engineered MSCs hold significant promise a potent tool for cell therapy.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"17 1","pages":"101485"},"PeriodicalIF":3.6,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752454/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Innovative hydrogel delivery of bone marrow stromal cell-derived exosomes for enhanced bone healing. 创新水凝胶递送骨髓基质细胞衍生外泌体,增强骨愈合。
IF 3.6 3区 医学
World journal of stem cells Pub Date : 2024-12-26 DOI: 10.4252/wjsc.v16.i12.1106
Yue Ding, Fang Lin, Xiao-Ting Liang
{"title":"Innovative hydrogel delivery of bone marrow stromal cell-derived exosomes for enhanced bone healing.","authors":"Yue Ding, Fang Lin, Xiao-Ting Liang","doi":"10.4252/wjsc.v16.i12.1106","DOIUrl":"10.4252/wjsc.v16.i12.1106","url":null,"abstract":"<p><p>Bone regeneration is a multifaceted process involving the well-coordinated interaction of cellular functions such as the regulation of inflammation, the formation of new blood vessels, and the development of bone tissue. Bone regeneration is a multifaceted process involving the well-coordinated interplay of multiple cellular activities, such as inflammation control, blood vessel and bone tissue. Zhang <i>et al</i> developed a multifunctional hydrogel system embedded with bone marrow stromal cell-derived exosomes to address the challenges of large bone defects. This innovative approach demonstrated the dual-role capability of bone marrow stromal cell-derived exosomes in directing cell fate by significantly enhancing both angiogenesis and osteogenic differentiation <i>in vitro</i>. The hydrogel system effectively promoted the polarization of macrophages towards the anti-inflammatory M2 phenotype, fostering an environment that supports bone repair. The effectiveness of this hydrogel was validated in a murine fracture model, which promoted significant bone regeneration and functional vascularization. Despite compelling evidence, this study highlights areas for further investigation, including detailed descriptions of experimental procedures, control group selection, long-term outcomes, and the evaluation of inflammation status <i>in vivo</i>. Addressing these limitations will enhance the robustness and impact of the findings.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"16 12","pages":"1106-1109"},"PeriodicalIF":3.6,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preliminary study on the preparation of lyophilized acellular nerve scaffold complexes from rabbit sciatic nerves with human umbilical cord mesenchymal stem cells. 人脐带间充质干细胞制备兔坐骨神经冻干脱细胞神经支架复合物的初步研究。
IF 3.6 3区 医学
World journal of stem cells Pub Date : 2024-12-26 DOI: 10.4252/wjsc.v16.i12.1047
Chuang Qian, Shang-Yu Guo, Zheng Xu, Zhi-Qiang Zhang, Hao-Dong Li, Hao Li, Xiong-Sheng Chen
{"title":"Preliminary study on the preparation of lyophilized acellular nerve scaffold complexes from rabbit sciatic nerves with human umbilical cord mesenchymal stem cells.","authors":"Chuang Qian, Shang-Yu Guo, Zheng Xu, Zhi-Qiang Zhang, Hao-Dong Li, Hao Li, Xiong-Sheng Chen","doi":"10.4252/wjsc.v16.i12.1047","DOIUrl":"10.4252/wjsc.v16.i12.1047","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;The gold standard of care for patients with severe peripheral nerve injury is autologous nerve grafting; however, autologous nerve grafts are usually limited for patients because of the limited number of autologous nerve sources and the loss of neurosensory sensation in the donor area, whereas allogeneic or xenografts are even more limited by immune rejection. Tissue-engineered peripheral nerve scaffolds, with the morphology and structure of natural nerves and complex biological signals, hold the most promise as ideal peripheral nerve \"replacements\".&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Aim: &lt;/strong&gt;To prepare allogenic peripheral nerve scaffolds using a low-toxicity decellularization method, and use human umbilical cord mesenchymal stem cells (hUC-MSCs) as seed cells to cultivate scaffold-cell complexes for the repair of injured peripheral nerves.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;After obtaining sciatic nerves from New Zealand rabbits, an optimal acellular scaffold preparation scheme was established by mechanical separation, varying lyophilization cycles, and trypsin and DNase digestion at different times. The scaffolds were evaluated by hematoxylin and eosin (HE) and luxol fast blue (LFB) staining. The maximum load, durability, and elastic modulus of the acellular scaffolds were assessed using a universal material testing machine. The acellular scaffolds were implanted into the dorsal erector spinae muscle of SD rats and the scaffold degradation and systemic inflammatory reactions were observed at 3 days, 1 week, 3 weeks, and 6 weeks following surgery to determine the histocompatibility between xenografts. The effect of acellular scaffold extracts on fibroblast proliferation was assessed using an MTT assay to measure the cytotoxicity of the scaffold residual reagents. In addition, the umbilical cord from cesarean section fetuses was collected, and the Wharton's jelly (WJ) was separated into culture cells and confirm the osteogenic and adipogenic differentiation of mesenchymal stem cells (MSCs) and hUC-MSCs. The cultured cells were induced to differentiate into Schwann cells by the antioxidant-growth factor induction method, and the differentiated cells and the myelinogenic properties were identified.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;The experiments effectively decellularized the sciatic nerve of the New Zealand rabbits. After comparing the completed acellular scaffolds among the groups, the optimal decellularization preparation steps were established as follows: Mechanical separation of the epineurium, two cycles of lyophilization-rewarming, trypsin digestion for 5 hours, and DNase digestion for 10 hours. After HE staining, no residual nuclear components were evident on the scaffold, whereas the extracellular matrix remained intact. LFB staining showed a significant decrease in myelin sheath composition of the scaffold compared with that before preparation. Biomechanical testing revealed that the maximum tensile strength, elastic modulu","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"16 12","pages":"1047-1061"},"PeriodicalIF":3.6,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dual role of extracellular vesicles in neurodegenerative diseases. 细胞外囊泡在神经退行性疾病中的双重作用。
IF 3.6 3区 医学
World journal of stem cells Pub Date : 2024-12-26 DOI: 10.4252/wjsc.v16.i12.1002
Arianna Scuteri, Elisabetta Donzelli
{"title":"Dual role of extracellular vesicles in neurodegenerative diseases.","authors":"Arianna Scuteri, Elisabetta Donzelli","doi":"10.4252/wjsc.v16.i12.1002","DOIUrl":"10.4252/wjsc.v16.i12.1002","url":null,"abstract":"<p><p>Extracellular vesicles (EVs) are cell-to-cell interaction tools that are attracting increasing interest in the literature in two opposing areas. In addition to their role in physiological development, there is growing evidence of their involvement in healing and protective processes. However, EVs also mediate pathological conditions, particularly contributing to the progression of several chronic diseases, such as neurodegenerative diseases. On the other hand, EVs also form the core of a new therapeutic strategy for neuroprotection, which is based on the administration of EVs derived from a wide range of donor cells. In particular, the possibility of obtaining numerous EVs from stem cells of different origins, which is feasible for therapeutic aims, is now under investigation. In this review, we focused on neurodegenerative diseases, in which EVs could have a propagative detrimental effect or could also be exploited to deliver protective factors. This review explores the different hypotheses concerning the dual role of EVs, with the aim of shedding light on the following question: Can vesicles be used to fight vesicle-propagated diseases?</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"16 12","pages":"1002-1011"},"PeriodicalIF":3.6,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669982/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of autophagy in mesenchymal stem cells. 自噬在间充质干细胞中的应用。
IF 3.6 3区 医学
World journal of stem cells Pub Date : 2024-12-26 DOI: 10.4252/wjsc.v16.i12.990
Min Chai, Chun-Yan Zhang, Shuai Chen, Da-Hai Xu
{"title":"Application of autophagy in mesenchymal stem cells.","authors":"Min Chai, Chun-Yan Zhang, Shuai Chen, Da-Hai Xu","doi":"10.4252/wjsc.v16.i12.990","DOIUrl":"10.4252/wjsc.v16.i12.990","url":null,"abstract":"<p><p>In this editorial, we have taken an in-depth look at the article published by Wan <i>et al</i>. The study showed that preconditioning mesenchymal stem cells (MSCs) protected them against programmed cell death, and increased their survival rate and therapeutic potential. Autophagy, a type of programmed cell death, is a major intracellular degradation and recycling pathway that is crucial for maintaining cellular homeostasis, self-renewal, and pluripotency. We have explored the relationship between autophagy and MSCs to determine the role of autophagy in the therapeutic applications of MSCs.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"16 12","pages":"990-1001"},"PeriodicalIF":3.6,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669988/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bibliometrics of trends in global research on the roles of stem cells in myocardial fibrosis therapy. 干细胞在心肌纤维化治疗中的作用的全球研究趋势的文献计量学。
IF 3.6 3区 医学
World journal of stem cells Pub Date : 2024-12-26 DOI: 10.4252/wjsc.v16.i12.1086
Jing-Yi Ding, Tian-Tian Meng, Ruo-Lin Du, Xin-Bin Song, Yi-Xiang Li, Jing Gao, Ran Ji, Qing-Yong He
{"title":"Bibliometrics of trends in global research on the roles of stem cells in myocardial fibrosis therapy.","authors":"Jing-Yi Ding, Tian-Tian Meng, Ruo-Lin Du, Xin-Bin Song, Yi-Xiang Li, Jing Gao, Ran Ji, Qing-Yong He","doi":"10.4252/wjsc.v16.i12.1086","DOIUrl":"10.4252/wjsc.v16.i12.1086","url":null,"abstract":"<p><strong>Background: </strong>Myocardial fibrosis, a condition linked to several cardiovascular diseases, is associated with a poor prognosis. Stem cell therapy has emerged as a potential treatment option and the application of stem cell therapy has been studied extensively. However, a comprehensive bibliometric analysis of these studies has yet to be conducted.</p><p><strong>Aim: </strong>To map thematic trends, analyze research hotspots, and project future directions of stem cell-based myocardial fibrosis therapy.</p><p><strong>Methods: </strong>We conducted a bibliometric and visual analysis of studies in the Web of Science Core Collection using VOSviewer and Microsoft Excel. The dataset included 1510 articles published between 2001 and 2024. Countries, organizations, authors, references, keywords, and co-citation networks were examined to identify evolving research trends.</p><p><strong>Results: </strong>Our findings revealed a steady increase in the number of publications, with a projected increase to over 200 publications annually by 2030. Initial research focused on stem cell-based therapy, particularly for myocardial infarction and heart failure. More recently, there has been a shift toward cell-free therapy, involving extracellular vesicles, exosomes, and microRNAs. Key research topics include angiogenesis, inflammation, apoptosis, autophagy, and oxidative stress.</p><p><strong>Conclusion: </strong>This analysis highlights the evolution of stem cell therapies for myocardial fibrosis, with emerging interest in cell-free approaches. These results are expected to guide future scientific exploration and decision-making.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"16 12","pages":"1086-1105"},"PeriodicalIF":3.6,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669986/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mesenchymal stem cell therapy in atherosclerosis: A bibliometric and visual analysis. 动脉粥样硬化的间充质干细胞治疗:文献计量学和视觉分析。
IF 3.6 3区 医学
World journal of stem cells Pub Date : 2024-12-26 DOI: 10.4252/wjsc.v16.i12.1062
Xing Cheng, Ya-Ling Li, Heng Wang, Rui-Jing Zhang, Ke-Yi Fan, Xiao-Tong Qi, Guo-Ping Zheng, Hong-Lin Dong
{"title":"Mesenchymal stem cell therapy in atherosclerosis: A bibliometric and visual analysis.","authors":"Xing Cheng, Ya-Ling Li, Heng Wang, Rui-Jing Zhang, Ke-Yi Fan, Xiao-Tong Qi, Guo-Ping Zheng, Hong-Lin Dong","doi":"10.4252/wjsc.v16.i12.1062","DOIUrl":"10.4252/wjsc.v16.i12.1062","url":null,"abstract":"<p><strong>Background: </strong>Mesenchymal stem cells (MSCs) are capable of self-renewal and differentiation, and extensive studies have demonstrated their therapeutic potential in atherosclerosis (AS).</p><p><strong>Aim: </strong>To conduct a bibliometric analysis of studies on the use of MSC therapy for AS over the past two decades, assess key trends and provide insights for future research directions.</p><p><strong>Methods: </strong>We systematically searched the Web of Science Core Collection database for articles published between 1999 and 2023, yielding a total of 556 articles. Visual representation and bibliometric analysis of information and trends were facilitated using CiteSpace, the R package 'bibliometrix' and VOSviewer.</p><p><strong>Results: </strong>The analyzed articles were predominantly from 52 countries/regions, with prominent contributions from China and the United States. A cohort of 3057 authors contributed to these publications, with the works of Libby P distinguished by their influence and citation count. <i>Int J Mol Sci</i> has emerged as the journal with the highest publication volume, prominently disseminating influential papers and identifying citation outbreaks. Furthermore, our analysis identified current research hotspots within the field, focusing on vascular progenitor cells, inflammatory mechanisms, and extracellular vesicles. Emerging research frontiers, such as extracellular vesicles and oxidative stress, have been highlighted as areas of burgeoning interest. Finally, we offer perspectives on the status of research and future directions of MSC therapy in AS.</p><p><strong>Conclusion: </strong>This comprehensive analysis provides valuable insights for advancing scientific research on MSC therapy for AS. By elucidating pivotal trends and research directions, this study aimed to foster innovation and promote the progress of disciplines in this field, thereby contributing to advancing scientific knowledge and clinical practice.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"16 12","pages":"1062-1085"},"PeriodicalIF":3.6,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preliminary evidence of renal function improvement in chronic progressive kidney disease using autologous CD34+ cell therapy: A clinical trial. 使用自体CD34+细胞治疗慢性进行性肾病肾功能改善的初步证据:一项临床试验
IF 3.6 3区 医学
World journal of stem cells Pub Date : 2024-12-26 DOI: 10.4252/wjsc.v16.i12.1012
Takayasu Ohtake, Tsutomu Sato, Toshitaka Tsukiyama, Suguru Muraoka, Ayaka Mitomo, Haruka Maruyama, Mizuki Yamano, Yasuhiro Mochida, Kunihiro Ishioka, Machiko Oka, Hidekazu Moriya, Sumi Hidaka, Haruchika Masuda, Takayuki Asahara, Shuzo Kobayashi
{"title":"Preliminary evidence of renal function improvement in chronic progressive kidney disease using autologous CD34<sup>+</sup> cell therapy: A clinical trial.","authors":"Takayasu Ohtake, Tsutomu Sato, Toshitaka Tsukiyama, Suguru Muraoka, Ayaka Mitomo, Haruka Maruyama, Mizuki Yamano, Yasuhiro Mochida, Kunihiro Ishioka, Machiko Oka, Hidekazu Moriya, Sumi Hidaka, Haruchika Masuda, Takayuki Asahara, Shuzo Kobayashi","doi":"10.4252/wjsc.v16.i12.1012","DOIUrl":"10.4252/wjsc.v16.i12.1012","url":null,"abstract":"<p><strong>Background: </strong>To date, no specific treatment has been established to reverse progressive chronic kidney disease (CKD).</p><p><strong>Aim: </strong>To evaluate the safety and efficacy of autologous CD34<sup>+</sup> cell transplantation in CKD patients who exhibited a progressive decline in renal function.</p><p><strong>Methods: </strong>The estimated glomerular filtration rate (eGFR) at the beginning of the study was 15.0-28.0 mL/minute/1.73 m<sup>2</sup>. After five days of treatment with the granulocyte colony-stimulating factor, mononuclear cells were harvested and CD34<sup>+</sup> cells were magnetically collected. CD34<sup>+</sup> cells were directly injected into the bilateral renal arteries twice (at 0 and 3 months), and their safety and efficacy were evaluated for 6 months.</p><p><strong>Results: </strong>Four patients were enrolled and completed the study. Three of four patients showed improvement in eGFR slope (eGFR slope > 0 mL/minute/1.73 m<sup>2</sup>), with the monthly slope of eGFR (delta eGFR) changing from -1.36 ± 1.1 (pretreatment) to +0.22 ± 0.71 (at 6 months) mL/minute/1.73 m<sup>2</sup>/month (<i>P</i> = 0.135) after cell therapy. Additionally, intrarenal resistive index (<i>P</i> = 0.004) and shear wave velocity (<i>P</i> = 0.04) were significantly improved after cell therapy. One patient experienced transient fever after cell therapy, and experienced bone pain during granulocyte colony-stimulating factor administration. However, no severe adverse events were reported.</p><p><strong>Conclusion: </strong>In conclusion, our findings suggest that repetitive peripheral blood-derived autologous CD34<sup>+</sup> cell transplantation into the renal arteries is safe, feasible, and may be effective for patients with progressive CKD. However, a large-scale clinical trial is warranted to validate the efficacy of repetitive regenerative cell therapy using autologous CD34<sup>+</sup> cells in patients with progressive CKD.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"16 12","pages":"1012-1021"},"PeriodicalIF":3.6,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Interplay and therapeutic implications of colorectal cancer stem cells, tumor microenvironment, and gut microbiota. 结直肠癌干细胞、肿瘤微环境和肠道微生物群的相互作用及其治疗意义。
IF 3.6 3区 医学
World journal of stem cells Pub Date : 2024-12-26 DOI: 10.4252/wjsc.v16.i12.1110
Hui Zhang, Bo-Tao Xu, Di-Ping Luo, Tie-Fei He
{"title":"Interplay and therapeutic implications of colorectal cancer stem cells, tumor microenvironment, and gut microbiota.","authors":"Hui Zhang, Bo-Tao Xu, Di-Ping Luo, Tie-Fei He","doi":"10.4252/wjsc.v16.i12.1110","DOIUrl":"10.4252/wjsc.v16.i12.1110","url":null,"abstract":"<p><p>This article discusses the interplay between colorectal cancer (CRC) stem cells, tumor microenvironment (TME), and gut microbiota, emphasizing their dynamic roles in cancer progression and treatment resistance. It highlights the adaptability of CRC stem cells, the bidirectional influence of TME, and the multifaceted impact of gut microbiota on CRC. The manuscript proposes innovative therapeutic strategies focusing on these interactions, advocating for a shift towards personalized and ecosystem-targeted treatments in CRC. The conclusion underscores the importance of continued research in these areas for developing effective, personalized therapies.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"16 12","pages":"1110-1114"},"PeriodicalIF":3.6,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exosomes derived from microRNA-540-3p overexpressing mesenchymal stem cells promote immune tolerance via the CD74/nuclear factor-kappaB pathway in cardiac allograft. 过表达microRNA-540-3p的间充质干细胞衍生的外泌体通过CD74/核因子- κ b途径促进同种异体心脏移植的免疫耐受。
IF 3.6 3区 医学
World journal of stem cells Pub Date : 2024-12-26 DOI: 10.4252/wjsc.v16.i12.1022
Ji-Gang He, Xin-Xin Wu, Si Li, Dan Yan, Gao-Peng Xiao, Fu-Gang Mao
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