World journal of stem cells最新文献

{"title":"Exosomal miR-203 from bone marrow stem cells targets the SOCS3/NF-κB pathway to regulate neuroinflammation in temporal lobe epilepsy.","authors":"Wei Wang, Jian Yin","doi":"10.4252/wjsc.v17.i2.101395","DOIUrl":"10.4252/wjsc.v17.i2.101395","url":null,"abstract":"<p><strong>Background: </strong>Epilepsy is a prevalent chronic neurological disorder affecting 50 million individuals globally, with temporal lobe epilepsy (TLE) being the most common form. Despite advances in antiepileptic drug development, over 30% of patients suffer from drug-resistant epilepsy, which can lead to severe cognitive impairments and adverse psychosocial outcomes.</p><p><strong>Aim: </strong>To explore the role of bone marrow mesenchymal stem cell (BMSC)-derived exosomal miR-203 in the regulation of neuroinflammation in a mouse model of epilepsy, providing a theoretical basis for the development of targeted microRNA delivery therapies for drug-resistant epilepsy.</p><p><strong>Methods: </strong>Adult male C57BL/6 mice were divided into a control group and a TLE model of 30 mice each, and the TLE model group was established by injecting kainic acid. BMSCs were isolated from the mice, and exosomes were purified using ultracentrifugation. Exosomal miR-203 was identified and characterized using high-throughput sequencing and quantitative reverse-transcription polymerase chain reaction. The uptake of exosomes by hippocampal neurons and the subsequent effects on neuroinflammatory markers were assessed using <i>in vitro</i> cell culture models.</p><p><strong>Results: </strong>Exosomal miR-203 exhibited a significant upregulation in BMSCs derived from epileptic mice. <i>In vitro</i> investigations demonstrated the efficient internalization of these exosomes by hippocampal neurons, resulting in downregulation of suppressor of cytokine signaling 3 expression and activation of the nuclear factor kappaB pathway, ultimately leading to enhanced secretion of pro-inflammatory cytokines.</p><p><strong>Conclusion: </strong>Our study identifies exosomal miR-203 as a key regulator of neuroinflammation in a mouse model of epilepsy. The findings suggest that targeting miR-203 may offer a novel therapeutic strategy for epilepsy by modulating the suppression of cytokine signaling 3/nuclear factor kappaB pathway, thus providing a potential avenue for the development of cell-free therapeutics.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"17 2","pages":"101395"},"PeriodicalIF":3.6,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perspectives on Schwann-like cells derived from bone marrow-mesenchymal stem cells: Advancing peripheral nerve injury therapies.","authors":"Lucas Vinícius de Oliveira Ferreira, Rogério Martins Amorim","doi":"10.4252/wjsc.v17.i2.102702","DOIUrl":"10.4252/wjsc.v17.i2.102702","url":null,"abstract":"<p><p>Peripheral nerve injuries are clinical conditions that often result in functional deficits, compromising patient quality of life. Given the relevance of these injuries, new treatment strategies are constantly being investigated. Although mesenchymal stem cells already demonstrate therapeutic potential due to their paracrine action, the transdifferentiation of these cells into Schwann-like cells (SLCs) represents a significant advancement in nerve injury therapy. Recent studies indicate that SLCs can mimic the functions of Schwann cells, with promising results in animal models. However, challenges remain, such as the diversity of transdifferentiation protocols and the scalability of these therapies for clinical applications. A recent study by Zou <i>et al</i> provided a comprehensive overview of the role of bone marrow-derived mesenchymal stem cells in the treatment of peripheral nerve injuries. Therefore, we would like to discuss and explore the use of SLCs derived from bone marrow-derived mesenchymal stem cells in more detail as a promising alternative in the field of nerve regeneration.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"17 2","pages":"102702"},"PeriodicalIF":3.6,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asthma and stem cell therapy.","authors":"Qiong-Hua Chen, Jing-Yang Zheng, Da-Chun Wang","doi":"10.4252/wjsc.v17.i2.103599","DOIUrl":"10.4252/wjsc.v17.i2.103599","url":null,"abstract":"<p><p>The global incidence of asthma, a leading respiratory disorder affecting more than 235 million people, has dramatically increased in recent years. Characterized by chronic airway inflammation and an imbalanced response to airborne irritants, this chronic condition is associated with elevated levels of inflammatory factors and symptoms such as dyspnea, cough, wheezing, and chest tightness. Conventional asthma therapies, such as corticosteroids, long-acting β-agonists, and anti-inflammatory agents, often evoke diverse adverse reactions and fail to reduce symptoms and hospitalization rates over the long term effectively. These limitations have prompted researchers to explore innovative therapeutic strategies, including stem cell-related interventions, offering hope to those afflicted with this incurable disease. In this review, we describe the characteristics of stem cells and critically assess the potential and challenges of stem cell-based therapies to improve disease management and treatment outcomes for asthma and other diseases.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"17 2","pages":"103599"},"PeriodicalIF":3.6,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885939/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fetal mice dermal mesenchymal stem cells promote wound healing by inducing M2 type macrophage polarization.","authors":"Zhen-Yu Xia, Yi Wang, Nian Shi, Mei-Qi Lu, Yun-Xiang Deng, Yong-Jun Qi, Xing-Lei Wang, Jie Zhao, Du-Yin Jiang","doi":"10.4252/wjsc.v17.i2.101030","DOIUrl":"10.4252/wjsc.v17.i2.101030","url":null,"abstract":"<p><strong>Background: </strong>Mesenchymal stem cells, found in various tissues, possess significant healing and immunomodulatory properties, influencing macrophage polarization, which is essential for wound repair. However, chronic wounds present significant therapeutic challenges, requiring novel strategies to improve healing outcomes.</p><p><strong>Aim: </strong>To investigate the potential of fetal dermal mesenchymal stem cells (FDMSCs) in enhancing wound healing through modulation of macrophage polarization, specifically by promoting the M2 phenotype to address inflammatory responses in chronic wounds.</p><p><strong>Methods: </strong>FDMSCs were isolated from BalB/C mice and co-cultured with RAW264.7 macrophages to assess their effects on macrophage polarization. Flow cytometry, quantitative reverse transcriptase polymerase chain reaction, and histological analyses were employed to evaluate shifts in macrophage phenotype and wound healing in a mouse model. Statistical analysis was performed using GraphPad Prism.</p><p><strong>Results: </strong>FDMSCs induced macrophage polarization from the M1 to M2 phenotype, as demonstrated by a reduction in pro-inflammatory markers (inducible nitric oxide synthase, interleukin-6) and an increase in anti-inflammatory markers [mannose receptor (CD206), arginase-1] in co-cultured RAW264.7 macrophages. These shifts were confirmed by flow cytometry. In an acute skin wound model, FDMSC-treated mice exhibited faster wound healing, enhanced collagen deposition, and improved vascular regeneration compared to controls. Significantly higher expression of arginase-1 further indicated an enriched M2 macrophage environment.</p><p><strong>Conclusion: </strong>FDMSCs effectively modulate macrophage polarization from M1 to M2, reduce inflammation, and enhance tissue repair, demonstrating their potential as an immunomodulatory strategy in wound healing. These findings highlight the promising therapeutic application of FDMSCs in managing chronic wounds.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"17 2","pages":"101030"},"PeriodicalIF":3.6,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"WDR36 inhibits the osteogenic differentiation and migration of periodontal ligament stem cells.","authors":"Yi Wang, Feng-Qiu Zhang, Zhi-Peng Fan, Xin-Ling Zhu, Wan-Hao Yan, Xiu-Li Zhang","doi":"10.4252/wjsc.v17.i2.99132","DOIUrl":"10.4252/wjsc.v17.i2.99132","url":null,"abstract":"<p><strong>Background: </strong>Periodontitis is an inflammatory disease caused by the host's immune response and various interactions between pathogens, which lead to the loss of connective tissue and bone. In recent years, mesenchymal stem cell (SC) transplantation technology has become a research hotspot, which can form periodontal ligament, cementum, and alveolar bone through proliferation and differentiation.</p><p><strong>Aim: </strong>To elucidate the regulatory effects of WD repeat-containing protein 36 (WDR36) on the senescence, migration, and osteogenic differentiation of periodontal ligament SCs (PDLSCs).</p><p><strong>Methods: </strong>The migration and chemotaxis of PDLSCs were detected by the scratch-wound migration test and transwell chemotaxis test. Alkaline phosphatase (ALP) activity, Alizarin red staining, calcium content, and real-time reverse transcription polymerase chain reaction (RT-qPCR) of key transcription factors were used to detect the osteogenic differentiation function of PDLSCs. Cell senescence was determined by senescence-associated β-galactosidase staining.</p><p><strong>Results: </strong>The 24-hour and 48-hour scratch-wound migration test and 48-hour transwell chemotaxis test showed that overexpression of WDR36 inhibited the migration/chemotaxis of PDLSCs. Simultaneously, WDR36 depletion promoted the migration/chemotaxis of PDLSCs. The results of ALP activity, Alizarin red staining, calcium content, and RT-qPCR showed that overexpression of WDR36 inhibited the osteogenic differentiation of PDLSCs, and WDR36 depletion promoted the osteogenic differentiation of PDLSCs. Senescence-associated β-galactosidase staining showed that 0.1 μg/mL icariin (ICA) and overexpression of WDR36 inhibited the senescence of PDLSCs, and WDR36 depletion promoted the osteogenic differentiation of PDLSCs.</p><p><strong>Conclusion: </strong>WDR36 inhibits the migration and chemotaxis, osteogenic differentiation, and senescence of PDLSCs; 0.1 μg/mL ICA inhibits the senescence of PDLSCs. Therefore, WDR36 might serve as a target for periodontal tissue regeneration and the treatment of periodontitis.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"17 2","pages":"99132"},"PeriodicalIF":3.6,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of adipose-derived stem cells exosomes cross-linked chitosan-αβ-glycerophosphate thermosensitive hydrogel on deep burn wounds.","authors":"Lei Xu, Dan Liu, Hai-Long Yun, Wei Zhang, Li Ren, Wen-Wen Li, Chuan Han","doi":"10.4252/wjsc.v17.i2.102091","DOIUrl":"10.4252/wjsc.v17.i2.102091","url":null,"abstract":"<p><strong>Background: </strong>Burn wound management is challenging, and while mesenchymal stem cell-derived exosomes show therapeutic potential, optimal delivery methods are unclear.</p><p><strong>Aim: </strong>To study chitosan (CS)-αβ-glycerophosphate (CS-αβ-GP) hydrogel crosslinked with adipose-derived stem cell exosomes (ASC-Exos) for healing deep burn injuries.</p><p><strong>Methods: </strong>Rats with deep burn injuries were divided into the CS + ASCs-Exos group, the ASCs-Exos group, the CS group, and the control group. The healing rates on days 4, 7, and 14 after treatment were analyzed using ImageJ software. On day 14, the tissues were stained with hematoxylin and eosin staining, Masson's trichrome staining, and immunohistochemical analysis to evaluate tumor necrosis factor α, interleukin-6 (IL-6), IL-1α, IL-10, transforming growth factor β, and epidermal growth factor. The mRNA levels of IL-1α, CD86, C-C motif chemokine ligand 22, and CD163 were evaluated through quantitative polymerase chain reaction.</p><p><strong>Results: </strong>The CS + ASC-Exos group exhibited enhanced healing, reduced lymphocyte infiltration, blood vessels, and muscle fiber distribution. Increased IL-10, transforming growth factor β, and epidermal growth factor and decreased tumor necrosis factor α, IL-1α, and IL-6 expression were observed. Quantitative polymerase chain reaction revealed reduced IL-1α and CD86 and increased C-C motif chemokine ligand 22 and CD163 expression. Protein analysis showed downregulation of phosphorylated inhibitor of kappa Balpha and P65 in the nuclear factor κB (NF-κB) pathway. ASC-Exos crosslinked with CS-αβ-GP hydrogel demonstrates superior effects in anti-inflammation, wound healing promotion, and promotion of M1 macrophage transformation to M2 macrophage by blocking the NF-κB pathway compared to ASC-Exos alone.</p><p><strong>Conclusion: </strong>Our research demonstrates that the ASC-Exos cross-linked CS-αβ-GP hydrogel represents an advanced therapeutic approach for treating deep burn wounds. It has anti-inflammatory effects, promotes wound healing, and facilitates the transition of M1 macrophages to M2 macrophages by blocking the NF-κB pathway.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"17 2","pages":"102091"},"PeriodicalIF":3.6,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of macrophages on the osteogenic differentiation of adipose tissue-derived stem cells in two-dimensional and three-dimensional cocultures.","authors":"He-Ao Zhang, Bo-Yu Zhang, Hong-Bo Tang","doi":"10.4252/wjsc.v17.i2.99326","DOIUrl":"10.4252/wjsc.v17.i2.99326","url":null,"abstract":"<p><strong>Background: </strong>Fracture is one of the most pervasive injuries in the musculoskeletal system, and there is a complex interaction between macrophages and adipose tissue-derived stem cells (ADSCs) in fracture healing. However, two-dimensional (2D) coculture of macrophages and ADSCs can not accurately mimic the <i>in vivo</i> cell microenvironment.</p><p><strong>Aim: </strong>To establish both 2D and 3D osteogenic coculture models to investigate the interaction between macrophages and ADSCs.</p><p><strong>Methods: </strong>After obtaining ADSCs from surgery and inducing differentiation of the THP1 cell line, we established 2D and 3D osteogenic coculture models. To assess the level of osteogenic differentiation, we used alizarin red staining and measured the relative expression levels of osteogenic differentiation markers osteocalcin, Runt-related transcription factor 2, and alkaline phosphatase through polymerase chain reaction. Verification was conducted by analyzing the expression changes of N-cadherin and the activation of the Wnt/β-catenin signaling pathway using western blotting.</p><p><strong>Results: </strong>In this study, it was discovered that macrophages in 3D culture inhibited osteogenic differentiation of ADSCs, contrary to the effect in 2D culture. This observation confirmed the significance of intricate intercellular connections in the 3D culture environment. Additionally, the 3D culture group exhibited significantly higher N-cadherin expression and showed reduced β-catenin and Wnt1 protein levels compared to the 2D culture group.</p><p><strong>Conclusion: </strong>Macrophages promoted ADSC osteogenic differentiation in 2D culture conditions but inhibited it in 3D culture. The 3D culture environment might inhibit the Wnt/β-catenin signaling pathway by upregulating N-cadherin expression, ultimately hindering the osteogenic differentiation of ADSCs. By investigating the process of osteogenesis in ADSCs, this study provides novel ideas for exploring 3D osteogenesis in ADSCs, fracture repair, and other bone trauma repair.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"17 2","pages":"99326"},"PeriodicalIF":3.6,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nicotinamide adenine dinucleotide rejuvenates septic bone marrow mesenchymal stem cells.","authors":"Xin Xia, Kun Zhou, Lin-Ying An, Min Zhao, Bin-Le Tian, Jin-Yan Zhao, Zhi-Gang Zhou, Yin Tong","doi":"10.4252/wjsc.v17.i2.96893","DOIUrl":"10.4252/wjsc.v17.i2.96893","url":null,"abstract":"<p><strong>Background: </strong>Sepsis is a severe illness characterized by systemic and multiorgan reactive responses and damage. However, the impact of sepsis on the bone marrow, particularly on bone marrow mesenchymal stem cells (BMSCs), is less reported. BMSCs are critical stromal cells in the bone marrow microenvironment that maintain bone stability and hematopoietic homeostasis; however, the impairment caused by sepsis remains unknown.</p><p><strong>Aim: </strong>To investigate the effects of sepsis on BMSCs and the underlying mechanisms.</p><p><strong>Methods: </strong>BMSCs were obtained from healthy donors and patients with sepsis. We compared the self-renewal capacity, differentiation potential, and hematopoietic supportive ability <i>in vitro</i>. Senescence of septic BMSCs was assessed using β-galactosidase staining, senescence-associated secretory phenotype, intracellular reactive oxygen species levels, and the expression of P16 and P21. Finally, the changes in septic BMSCs after nicotinamide adenine dinucleotide (NAD) treatment were evaluated.</p><p><strong>Results: </strong>Septic BMSCs showed decreased proliferation and self-renewal, bias towards adipogenic differentiation, and weakened osteogenic differentiation. Additionally, hematopoietic supportive capacity declines in sepsis. The levels of aging markers were significantly higher in the septic BMSCs. After NAD treatment, the proliferation capacity of septic BMSCs showed a recovery trend, with increased osteogenic and hematopoietic supportive capacities. Sepsis resulted in decreased expression of sirtuin 3 (SIRT3) in BMSCs, whereas NAD treatment restored SIRT3 expression, enhanced superoxide dismutase enzyme activity, reduced intracellular reactive oxygen species levels, maintained mitochondrial stability and function, and ultimately rejuvenated septic BMSCs.</p><p><strong>Conclusion: </strong>Sepsis accelerates the aging of BMSCs, as evidenced by a decline in self-renewal and osteogenic capabilities, as well as weakened hematopoietic support functions. These deficiencies can be effectively reversed <i>via</i> the NAD/SIRT3/superoxide dismutase pathway.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"17 2","pages":"96893"},"PeriodicalIF":3.6,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induced pluripotent stem cell-derived mesenchymal stem cells for modeling and treating metabolic associated fatty liver disease and metabolic associated steatohepatitis: Challenges and opportunities.","authors":"Bárbara Silva, José Bragança","doi":"10.4252/wjsc.v17.i2.99331","DOIUrl":"10.4252/wjsc.v17.i2.99331","url":null,"abstract":"<p><p>The potential of induced pluripotent stem cells (iPSCs) for modeling and treating metabolic associated fatty liver disease (MAFLD) and metabolic associated steatohepatitis (MASH) is emerging. MAFLD is a growing global health concern, currently with limited treatment options. While primary mesenchymal stem cells hold promise, iPSCs offer a versatile alternative due to their ability to differentiate into various cell types, including iPSC-derived mesenchymal stem cells. However, challenges remain, including optimizing differentiation protocols, ensuring cell safety, and addressing potential tumorigenicity risks. In addition, iPSCs offer the possibility to generate complex cellular models, including three-dimensional organoid models, which are closer representations of the human disease than animal models. Those models would also be valuable for drug discovery and personalized medicine approaches. Overall, iPSCs and their derivatives offer new perspectives for advancing MAFLD/MASH research and developing novel therapeutic strategies. Further research is needed to overcome current limitations and translate this potential into effective clinical applications.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"17 2","pages":"99331"},"PeriodicalIF":3.6,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of miR-214-5p and miR-21-5p from hypoxic endometrial exosomes on human umbilical cord mesenchymal stem cell function.","authors":"Jin-Wei Zhang","doi":"10.4252/wjsc.v17.i2.102404","DOIUrl":"10.4252/wjsc.v17.i2.102404","url":null,"abstract":"<p><p>Exosomes derived from hypoxic endometrial epithelial cells are pivotal in cellular communication and tissue repair, offering new perspectives on reproductive health. This manuscript highlights the study by Zhang <i>et al</i>, which investigates the effects of miR-214-5p and miR-21-5p in hypoxic cell-derived exosomes on human umbilical cord mesenchymal stem cells. The study reveals that low levels of these microRNAs activate the signal transducer and activator of transcription 3 signaling pathway, enhancing human umbilical cord mesenchymal stem cell migration and differentiation. These findings provide novel insights into therapeutic strategies for improving endometrial health and addressing infertility linked to thin endometrium.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"17 2","pages":"102404"},"PeriodicalIF":3.6,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}