World journal of stem cells最新文献

{"title":"Stromal vascular fraction: Mechanisms and application in reproductive disorders.","authors":"Zhi-Han Liu, Qi-Qi Xie, Jia-Lyu Huang","doi":"10.4252/wjsc.v17.i1.101097","DOIUrl":"10.4252/wjsc.v17.i1.101097","url":null,"abstract":"<p><p>Stromal vascular fraction (SVF) is a complex mixture derived from adipose tissue, consisting of a variety of cells. Due to its potential for tissue repair, immunomodulation, and support of angiogenesis, SVF represents a promising frontier in regenerative medicine and offers potential therapy for a range of disease conditions. In this article, we delve into the mechanisms through which SVF exerts its effects and explore its potential applications in treating both male and female reproductive disorders, including erectile dysfunction, testicular injury, stress urinary incontinence and intrauterine adhesion.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"17 1","pages":"101097"},"PeriodicalIF":3.6,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin regulation and the function of the periodontal ligament: Future perspective and challenges.","authors":"Andrea Scribante","doi":"10.4252/wjsc.v17.i1.101347","DOIUrl":"10.4252/wjsc.v17.i1.101347","url":null,"abstract":"<p><p>The present article reviews the emerging role of melatonin (MT) and the Hippo-Yes-associated protein signaling pathway in periodontal regeneration, highlighting their potential to delay the aging process of periodontal ligament stem cells (PDLSCs). Oxidative stress and cellular senescence are major obstacles in regenerative therapies, especially in an aging population. MT, a potent antioxidant, restores the morphology, proliferation, and osteogenic differentiation potential of PDLSCs under oxidative stress conditions. Recent research highlights how MT enhances PDLSC stemness by upregulating Yes-associated protein expression, offering a promising therapeutic strategy to antagonize tissue degeneration. In addition, the article discusses the growing interest in probiotics as a complementary approach to improve oral microbiota and support tissue regeneration. The integration of MT with traditional and novel therapeutic approaches may pave the way for innovative preventive or active treatments in periodontology, aimed at reducing oxidative stress. Future research needs to focus on translating these findings into clinical applications and promoting a deeper understanding of periodontal regeneration and cellular aging.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"17 1","pages":"101347"},"PeriodicalIF":3.6,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immortalization of epidural fat-derived mesenchymal stem cells: <i>In vitro</i> characterization and adipocyte differentiation potential.","authors":"Seoung-Woo Lee, Young-Ju Lim, Hee-Yeon Kim, Wansoo Kim, Wook-Tae Park, Min-Jung Ma, Junho Lee, Min-Soo Seo, Young In Kim, Sangbum Park, Seong-Kyoon Choi, Gun Woo Lee","doi":"10.4252/wjsc.v17.i1.98777","DOIUrl":"10.4252/wjsc.v17.i1.98777","url":null,"abstract":"<p><strong>Background: </strong>Mesenchymal stem cells (MSCs) are promising candidates for regenerative therapy due to their self-renewal capability, multilineage differentiation potential, and immunomodulatory effects. The molecular characteristics of MSCs are influenced by their location. Recently, epidural fat (EF) and EF-derived MSCs (EF-MSCs) have garnered attention due to their potential benefits to the spinal microenvironment and their high expression of neural SC markers. However, their clinical applications are limited due to cell senescence and limited accessibility of EF. Although many studies have attempted to establish an immortalized, stable SC line, the characteristics of immortalized EF-MSCs remain to be clarified.</p><p><strong>Aim: </strong>To establish and analyze stable immortalized EF-MSCs.</p><p><strong>Methods: </strong>The phenotypes of EF-MSCs were analyzed using optical microscopy. Cell immortalization was performed using lentiviral vectors. The biomolecular characteristics of the cells were analyzed by immunoblotting, quantitative PCR, and proteomics.</p><p><strong>Results: </strong>The immortalized EF-MSCs demonstrated a significantly extended lifespan compared to the control group, with well-preserved adipogenic potential and SC surface marker expression. Introduction of human telomerase reverse transcriptase genes markedly increased the lifespan of EF-MSCs. Proteomics analysis revealed substantial increase in the expression of DNA replication pathway components in immortalized EF-MSCs.</p><p><strong>Conclusion: </strong>Immortalized EF-MSCs exhibited significantly enhanced proliferative capacity, retained adipogenic potential, and upregulated the expression of DNA replication pathway components.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"17 1","pages":"98777"},"PeriodicalIF":3.6,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microvesicles derived from mesenchymal stem cells: A promising therapeutic strategy for acute respiratory distress syndrome-related pulmonary fibrosis?","authors":"Zhao Zhang, Xin-Yun Shan, Ce Liang, Lan Zhao, Xiao-Qian Shan","doi":"10.4252/wjsc.v17.i1.101036","DOIUrl":"10.4252/wjsc.v17.i1.101036","url":null,"abstract":"<p><p>Pulmonary fibrosis significantly contributes to the pathogenesis of acute respiratory distress syndrome (ARDS), markedly increasing patient mortality. Despite the established anti-fibrotic effects of mesenchymal stem cells (MSCs), numerous challenges hinder their clinical application. A recent study demonstrated that microvesicles (MVs) from MSCs (MSC-MVs) could attenuate ARDS-related pulmonary fibrosis and enhance lung function <i>via</i> hepatocyte growth factor mRNA transcription. This discovery presents a promising strategy for managing ARDS-associated pulmonary fibrosis. This article initially examines the safety and efficacy of MSCs from both basic science and clinical perspectives, subsequently exploring the potential and obstacles of employing MSC-MVs as a novel therapeutic approach. Additionally, it provides perspectives on future research into the application of MSC-MVs in ARDS-associated pulmonary fibrosis.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"17 1","pages":"101036"},"PeriodicalIF":3.6,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752460/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective activity of adipose-derived stem cell extracellular vesicles in ischemia and/or reperfusion.","authors":"Alexander E Berezin","doi":"10.4252/wjsc.v17.i1.102280","DOIUrl":"10.4252/wjsc.v17.i1.102280","url":null,"abstract":"<p><p>Increasing evidence of the significant clinical value of protection against ischemia/reperfusion injury has contributed to the realization of the independent importance of this approach in improving prognosis and reducing cardiovascular mortality. Extracellular vesicles (EVs) derived by adipose mesenchymal stem cells may mediate the paracrine effects of stem cells and provide regenerative and anti-inflammatory properties, which are enhanced by γ-aminobutyric acid. The protective effects on cardiac myocytes may result from the EV embarked by miR-21-5p, which is a target for thioredoxin-interacting protein, regulating the formation of thioredoxin-interacting protein-thioredoxin complexes and subsequently enhancing the antioxidant activity of thioredoxin. It has been found that γ-aminobutyric acid governs myocardial bioenergetics through suppressing inflammation and supporting mitochondrial structure. Finally, stem cell-based cell-free therapy based on adipose-derived stem cell EVs is considered a promising approach to individualized management of ischemia-induced cardiomyopathy.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"17 1","pages":"102280"},"PeriodicalIF":3.6,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752461/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in human umbilical cord mesenchymal stem cells-derived extracellular vesicles and biomaterial assemblies for endometrial injury treatment.","authors":"Wan-Yu Zhang, Han-Bi Wang, Cheng-Yan Deng","doi":"10.4252/wjsc.v17.i1.97905","DOIUrl":"10.4252/wjsc.v17.i1.97905","url":null,"abstract":"<p><p>Endometrial injury caused by repeated uterine procedures, infections, inflammation, or uterine artery dysfunction can deplete endometrial stem/progenitor cells and impair regeneration, thereby diminishing endometrial receptivity and evidently lowering the live birth, clinical pregnancy, and embryo implantation rates. Currently, safe and effective clinical treatment methods or gene-targeted therapies are unavailable, especially for severe endometrial injury. Umbilical cord mesenchymal stem cells and their extracellular vesicles are characterized by their simple collection, rapid proliferation, low immunogenicity, and tumorigenicity, along with their involvement in regulating angiogenesis, immune response, cell apoptosis and proliferation, inflammatory response, and fibrosis, Therefore, these cells and vesicles hold broad potential for application in endometrial repair. This article reviewed recent research on human umbilical cord mesenchymal stem cells as well as their extracellular vesicles in repairing endometrial injury.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"17 1","pages":"97905"},"PeriodicalIF":3.6,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone marrow mesenchymal stem cells promote uterine healing by activating the PI3K/AKT pathway and modulating inflammation in rat models.","authors":"Jing Yang, Jun Yuan, Yan-Qing Wen, Li Wu, Jiu-Jiang Liao, Hong-Bo Qi","doi":"10.4252/wjsc.v17.i1.98349","DOIUrl":"10.4252/wjsc.v17.i1.98349","url":null,"abstract":"<p><strong>Background: </strong>Uterine injury can cause uterine scarring, leading to a series of complications that threaten women's health. Uterine healing is a complex process, and there are currently no effective treatments. Although our previous studies have shown that bone marrow mesenchymal stem cells (BMSCs) promote uterine damage repair, the underlying mechanisms remain unclear. However, exploring the specific regulatory roles of BMSCs in uterine injury treatment is crucial for further understanding their functions and enhancing therapeutic efficacy.</p><p><strong>Aim: </strong>To investigate the underlying mechanism by which BMSCs promote the process of uterine healing.</p><p><strong>Methods: </strong>In <i>in vivo</i> experiments, we established a model of full-thickness uterine injury and injected BMSCs into the uterine wound. Transcriptome sequencing was performed to determine the enrichment of differentially expressed genes at the wound site. In <i>in vitro</i> experiments, we isolated rat uterine smooth muscle cells (USMCs) and cocultured them with BMSCs to observe the interaction between BMSCs and USMCs in the microenvironment.</p><p><strong>Results: </strong>We found that the differentially expressed genes were mainly related to cell growth, tissue repair, and angiogenesis, while the phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) pathway was highly enriched. Quantitative reverse-transcription polymerase chain reaction was used to validate differentially expressed genes, and the results demonstrated that BMSCs can upregulate genes related to regeneration and downregulate genes related to inflammation. Coculturing BMSCs promoted the migration and proliferation of USMCs, and the USMC microenvironment promoted the myogenic differentiation of BMSCs. Finally, we validated the PI3K/AKT pathway in tissues and cells and showed that BMSCs activate the PI3K/AKT pathway to promote the regeneration of uterine smooth muscle both <i>in vivo</i> and <i>in vitro</i>.</p><p><strong>Conclusion: </strong>BMSCs upregulated uterine wound regeneration and anti-inflammatory factors and enhanced uterine smooth muscle proliferation through the PI3K/AKT pathway both <i>in vivo</i> and <i>in vitro</i>.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"17 1","pages":"98349"},"PeriodicalIF":3.6,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bridging bioengineering and nanotechnology: Bone marrow derived mesenchymal stem cell-exosome solutions for peripheral nerve injury.","authors":"Jun Chang, Xiu-Mei Yin, Man Zhang, Jian-Wei Liu, Lan Zhao","doi":"10.4252/wjsc.v17.i1.101161","DOIUrl":"10.4252/wjsc.v17.i1.101161","url":null,"abstract":"<p><p>Peripheral nerve injury (PNI) is a common disease that is difficult to nerve regeneration with current therapies. Fortunately, Zou <i>et al</i> demonstrated the role and mechanism of bone marrow derived mesenchymal stem cells (BMSCs) in promoting nerve regeneration, revealing broad prospects for BMSCs transplantation in alleviating PNI. We confirmed the fact that BMSCs significantly alleviate PNI, but there are shortcomings such as low cell survival rate and immune rejection, which limit the wide application of BMSCs. BMSCs-derived exosomes (Exos) are considered as a promising cell-free nanomedicine for PNI, avoiding the ethical issues of BMSCs. Exos in combination with bioengineering therapeutics (including extracellular matrix, hydrogel) brings new hope for PNI, provides a favorable microenvironment for neurological restoration and a therapeutic strategy with a favorable safety profile, significantly increases expression of neurotrophic factors, promotes axonal and myelin regeneration, and demonstrates a strong potential to enhance neurogenesis. Therefore, engineered Exos exhibit better properties, such as stronger targeting and more beneficial components. This article briefly describes the role of nanotechnology and bioengineering therapies for BMSCs in PNI, proposes clinical application prospects and challenges of nanotechnology and bioengineering BMSCs-derived Exos in PNI to improve the efficacy of BMSCs in the treatment of PNI.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"17 1","pages":"101161"},"PeriodicalIF":3.6,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extended protective effects of three dimensional cultured human mesenchymal stromal cells in a neuroinflammation model.","authors":"Ok-Hyeon Kim, Hana Kang, Eun Seo Chang, Younghyun Lim, Young-Jin Seo, Hyun Jung Lee","doi":"10.4252/wjsc.v17.i1.101485","DOIUrl":"10.4252/wjsc.v17.i1.101485","url":null,"abstract":"<p><strong>Background: </strong>Human mesenchymal stromal cells (MSCs) possess regenerative potential due to pluripotency and paracrine functions. However, their stemness and immunomodulatory capabilities are sub-optimal in conventional two-dimensional (2D) culture.</p><p><strong>Aim: </strong>To enhance the efficiency and therapeutic efficacy of MSCs, an <i>in vivo</i>-like 3D culture condition was applied.</p><p><strong>Methods: </strong>MSCs were cultured on polystyrene (2D) or in a gellan gum-based 3D system. <i>In vitro</i>, prostaglandin-endoperoxide synthase 2, indoleamine-2,3-dioxygenase, heme oxygenase 1, and prostaglandin E synthase gene expression was quantified by quantitative real-time polymerase chain reaction. MSCs were incubated with lipopolysaccharide (LPS)-treated mouse splenocytes, and prostaglandin E2 and tumor necrosis factor-alpha levels were measured by enzyme linked immunosorbent assay. <i>In vivo</i>, LPS was injected into the lateral ventricle of mouse brain, and MSCs were administered intravenously the next day. Animals were sacrificed and analyzed on days 2 and 6.</p><p><strong>Results: </strong>Gellan gum polymer-based 3D culture significantly increased expression of octamer-binding transcription factor 4 and Nanog homeobox stemness markers in human MSCs compared to 2D culture. This 3D environment also heightened expression of cyclooxygenase-2 and heme-oxygenase 1, enzymes known for immunomodulatory functions, including production of prostaglandins and heme degradation, respectively. MSCs in 3D culture secreted more prostaglandin E2 and effectively suppressed tumor necrosis factor-alpha release from LPS-stimulated splenocytes and surpassed the efficiency of MSCs cultured in 2D. In a murine neuroinflammation model, intravenous injection of 3D-cultured MSCs significantly reduced ionized calcium-binding adaptor molecule 1 and glial fibrillary acidic protein expression, mitigating chronic inflammation more effectively than 2D-cultured MSCs.</p><p><strong>Conclusion: </strong>The microenvironment established in 3D culture serves as an <i>in vivo</i> mimetic, enhancing the immunomodulatory function of MSCs. This suggests that engineered MSCs hold significant promise a potent tool for cell therapy.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"17 1","pages":"101485"},"PeriodicalIF":3.6,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752454/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative hydrogel delivery of bone marrow stromal cell-derived exosomes for enhanced bone healing.","authors":"Yue Ding, Fang Lin, Xiao-Ting Liang","doi":"10.4252/wjsc.v16.i12.1106","DOIUrl":"10.4252/wjsc.v16.i12.1106","url":null,"abstract":"<p><p>Bone regeneration is a multifaceted process involving the well-coordinated interaction of cellular functions such as the regulation of inflammation, the formation of new blood vessels, and the development of bone tissue. Bone regeneration is a multifaceted process involving the well-coordinated interplay of multiple cellular activities, such as inflammation control, blood vessel and bone tissue. Zhang <i>et al</i> developed a multifunctional hydrogel system embedded with bone marrow stromal cell-derived exosomes to address the challenges of large bone defects. This innovative approach demonstrated the dual-role capability of bone marrow stromal cell-derived exosomes in directing cell fate by significantly enhancing both angiogenesis and osteogenic differentiation <i>in vitro</i>. The hydrogel system effectively promoted the polarization of macrophages towards the anti-inflammatory M2 phenotype, fostering an environment that supports bone repair. The effectiveness of this hydrogel was validated in a murine fracture model, which promoted significant bone regeneration and functional vascularization. Despite compelling evidence, this study highlights areas for further investigation, including detailed descriptions of experimental procedures, control group selection, long-term outcomes, and the evaluation of inflammation status <i>in vivo</i>. Addressing these limitations will enhance the robustness and impact of the findings.</p>","PeriodicalId":23775,"journal":{"name":"World journal of stem cells","volume":"16 12","pages":"1106-1109"},"PeriodicalIF":3.6,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}