Efficacy and safety of umbilical cord-derived mesenchymal stem cell-conditioned media for preventing and treating skin aging.

IF 3.6 3区医学 Q3 CELL & TISSUE ENGINEERING

World journal of stem cells Pub Date : 2025-09-26 DOI:10.4252/wjsc.v17.i9.108049

Hyunjun Ahn, Ho-Seong Han, Kye-Ho Lee

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引用次数: 0

Abstract

Background: Research has been increasingly conducted on the connection between mesenchymal stem cell (MSC)-conditioned medium (MSC-CM) and aging. However, most studies have focused on adipose-derived MSC-CM (ADMSC-CM), resulting in a research bias. We hypothesized that umbilical cord-derived MSCs, being younger than adipose-derived MSCs, would be more suitable for overcoming aging-related processes.

Aim: To assess the efficacy and safety of umbilical cord-derived MSC-CM (UCMSC-CM) for preventing and treating skin aging.

Methods: In vitro and in vivo studies were conducted to compare UCMSC-CM with ADMSC-CM, the most studied active aging-preventive conditioned medium to date. Additionally, the most effective delivery method of UCMSC-CM for aged skin was identified.

Results: UCMSC-CM had a higher content of effective factors, stimulated higher proliferation of fibroblasts, and strongly inhibited melanin production in B16F1 cells. In aged mice, UCMSC-CM application increased skin thickness, the number of Ki-67-positive cells, and the area of collagen deposition. UCMSC-CM was more effective than ADMSC-CM in preventing and treating skin aging. Additionally, a safety evaluation of UCMSC-CM performed in various animal models indicated that it was safe even when used directly on the skin.

Conclusion: UCMSC-CM is effective and safe for preventing and treating skin aging.

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脐带源性间充质干细胞条件培养基预防和治疗皮肤老化的有效性和安全性。

背景：关于间充质干细胞(MSC)-conditioned medium, MSC- cm)与衰老之间关系的研究越来越多。然而，大多数研究都集中在脂肪来源的MSC-CM （ADMSC-CM）上，导致研究偏差。我们假设脐带来源的间充质干细胞比脂肪来源的间充质干细胞更年轻，更适合克服衰老相关过程。目的：评价脐带源性间充质干细胞（UCMSC-CM）预防和治疗皮肤衰老的疗效和安全性。方法：采用体外和体内研究方法，比较UCMSC-CM与迄今为止研究最多的活性抗衰老条件培养基ADMSC-CM。此外，还确定了UCMSC-CM对衰老皮肤最有效的给药方法。结果：UCMSC-CM有效因子含量较高，能促进成纤维细胞增殖，对B16F1细胞黑色素生成有较强的抑制作用。在老年小鼠中，应用UCMSC-CM可增加皮肤厚度、ki -67阳性细胞数量和胶原沉积面积。UCMSC-CM在预防和治疗皮肤衰老方面优于ADMSC-CM。此外，在各种动物模型中对UCMSC-CM进行的安全性评估表明，即使直接在皮肤上使用，它也是安全的。结论：UCMSC-CM对预防和治疗皮肤老化具有安全、有效的作用。

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来源期刊

World journal of stem cells Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

7.80

自引率

4.90%

发文量

750

期刊介绍： The World Journal of Stem Cells (WJSC) is a leading academic journal devoted to reporting the latest, cutting-edge research progress and findings of basic research and clinical practice in the field of stem cells. It was launched on December 31, 2009 and is published monthly (12 issues annually) by BPG, the world''s leading professional clinical medical journal publishing company.