World Journal of Traditional Chinese Medicine最新文献

{"title":"Study on the mechanism and molecular docking verification of buyang huanwu decoction in treating diabetic foot","authors":"Da-Yuan Zhong, Lan-Hua Li, Huan Li, Ruofei Ma, Yi-Hui Deng","doi":"10.4103/2311-8571.370108","DOIUrl":"https://doi.org/10.4103/2311-8571.370108","url":null,"abstract":"Objective: The objective of this study was to investigate the molecular mechanism of Buyang Huanwu decoction (BYHWD) in the treatment of diabetic foot (DF). Methods: The TCMSP, BATMAN, PubChem, PharmMapper, UniProt, GeneCards, Webgestalt, and Kobas databases were used to obtain the structures, targets, main biological functions, and pathways of the active ingredients of BYHWD, and the results were visualized using Cytoscape3.6.1, Ledock, and PyMol software. Results: A total of 82 active components of BYHWD and 193 targets related to BYHWD were identified, and 5295 genes related to DF were identified using the GeneCards database, including 65 key targets of BYHWD in the treatment of DF. GO and KEGG enrichment analyses of the 65 targets for BYHWD treatment of DF showed that 47 GO items were involved in the treatment. It was mainly involved in biological processes, such as biological regulation, metabolism, and stress response. It is primarily involved in protein binding, ion binding, nucleotide binding, and other molecular functions. It is mainly involved in membrane encapsulation, membrane lumen closure, and other biological components and involved in the VEGF, TNF, RAS, RAP1, PI3K-AKT, MAPK, and IL-17 signaling pathways. Most targets were enriched in the PI3K-AKT and MAPK signaling pathways. Molecular docking results showed that the 59 key active components of BYHWD had strong binding activity with 64 key DF targets. Conclusion: The therapeutic effect of BYHWD on DF is based on the pharmacological effects of multiple targets and pathways.","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":"9 1","pages":"178 - 190"},"PeriodicalIF":4.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47898168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of acupuncture at Tianshu (ST25) and Shangjuxu (ST37) on the intestinal mucosa and metabolites of local skin tissues at Tianshu (ST25) in 2,4,6-Trinitrobenzene sulfonic acid and ethanol-induced rats","authors":"Jing-Ying Zhou, Yi-Tian Lai, Ling Ren, Lei Lan, Guo-shan Zhang, Mi Liu","doi":"10.4103/2311-8571.372729","DOIUrl":"https://doi.org/10.4103/2311-8571.372729","url":null,"abstract":"Objective: To observe the effect of acupuncture on local skin tissue metabolites of Tianshu (ST25) in rats with Crohn's disease (CD) using metabolomics and to explore the mechanism of acupuncture treatment metabolites and pathway on local skin tissue of Tianshu (ST25). Materials and Methods: Thirty-eight Sprague − Dawley rats were randomly assigned to a normal group (n = 14) and a CD modeling group (n = 24). Rats in the CD modeling group were administered with 2,4,6-trinitrobenzene sulfonic acid and ethanol enema, in addition to the normal group. Four normal rats and four CD modeling rats were selected at random after model identification was established. Furthermore, CD modeling group rats were randomly assigned to two groups of 10 rats: the model group and acupuncture group. Rats in the acupuncture group underwent acupuncture of Tianshu (ST25) and Shangjuxu (ST37) for 7 days. After the intervention, the colon tissue was collected from each group of rats and the pathological changes were observed through hematoxylin and eosin (HE) staining. The local skin tissues at Tianshu (ST25) of the rats in each group were taken, and the proton nuclear magnetic resonance technique was used to detect the metabonomics of the local skin tissues of Tianshu (ST25) in rats. Results: Compared with the normal group, the following were observed in the model group after HE staining: irregular colon morphology and other pathological changes such as intestinal mucosal hyperemia, edema, ulcers, polyps, and inflammatory cell infiltration. In the acupuncture group, colon tissue structure was relatively complete and layered, the colon gland structure was restored, and inflammatory cell infiltration was significantly improved. The choline, glycerin, glycine, guanidoacetic acid, and proline levels were significantly higher in the model group than in the normal group (P < 0.05 or P < 0.01). Compared with the model group, the acupuncture group had significantly increased contents of alanine, leucine, L-phenylalanine, and tyrosine in the skin (P < 0.05 or P < 0.01). Valine, leucine, and L-isoleucine biosynthesis and L-phenylalanine metabolism were the main metabolic pathways involved in the changes in the local skin tissues of the rats in each group, biosynthesis of tyrosine, and tryptophan. Conclusion: The change in the metabolites in the local area of Tianshu (ST25) may be related to intestinal disease. The acupuncture of Tianshu (ST25) can improve intestinal inflammatory reaction in rats with CD, and this finding may be related to the regulation of amino acid metabolites and their pathways in the local acupoint tissues of Tianshu (ST25) by acupuncture.","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":"9 1","pages":"131 - 140"},"PeriodicalIF":4.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41425477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traditional chinese medicine synonymous term conversion: A bidirectional encoder representations from transformers-based model for converting synonymous terms in traditional chinese medicine","authors":"Lu Zhou, Chaohong Wu, Xi-Ting Wang, Shuangqiao Liu, Yizhuo Zhang, Yue-Meng Sun, Jian Cui, Caiyan Li, Hui-Min Yuan, Yan Sun, Feng-jie Zheng, Feng-qin Xu, Yuhang Li","doi":"10.4103/2311-8571.378171","DOIUrl":"https://doi.org/10.4103/2311-8571.378171","url":null,"abstract":"Background: The medical records of traditional Chinese medicine (TCM) contain numerous synonymous terms with different descriptions, which is not conducive to computer-aided data mining of TCM. However, there is a lack of models available to normalize synonymous TCM terms. Therefore, construction of a synonymous term conversion (STC) model for normalizing synonymous TCM terms is necessary. Methods: Based on the neural networks of bidirectional encoder representations from transformers (BERT), four types of TCM STC models were designed: Models based on BERT and text classification, text sequence generation, named entity recognition, and text matching. The superior STC model was selected on the basis of its performance in converting synonymous terms. Moreover, three misjudgment inspection methods for the conversion results of the STC model based on inconsistency were proposed to find incorrect term conversion: Neuron random deactivation, output comparison of multiple isomorphic models, and output comparison of multiple heterogeneous models (OCMH). Results: The classification-based STC model outperformed the other STC task models. It achieved F1 scores of 0.91, 0.91, and 0.83 for performing symptoms, patterns, and treatments STC tasks, respectively. The OCMH method showed the best performance in misjudgment inspection, with wrong detection rates of 0.80, 0.84, and 0.90 in the term conversion results for symptoms, patterns, and treatments, respectively. Conclusion: The TCM STC model based on classification achieved superior performance in converting synonymous terms for symptoms, patterns, and treatments. The misjudgment inspection method based on OCMH showed superior performance in identifying incorrect outputs.","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":"9 1","pages":"224 - 233"},"PeriodicalIF":4.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45728451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of herb-partitioned moxibustion on structure and functional prediction of gut microbiota in rats with irritable bowel syndrome with diarrhea","authors":"Xia Liu, Jianan Cao, Tao Liu, H. Zhong, Mi Liu, X. Chang, Qiong Liu","doi":"10.4103/2311-8571.373586","DOIUrl":"https://doi.org/10.4103/2311-8571.373586","url":null,"abstract":"Objective: The objective of this study was to observe the effect of herb-partitioned moxibustion (HPM) on the gut microbiota of rats with diarrhea-predominant irritable bowel syndrome (IBS-D). Materials and Methods: A total of 48 male rats were randomly divided into a normal control group and an irritable bowel syndrome (IBS) model group. Using acetic acid irrigation and constraint stress, an IBS-D rat model was developed. After the model was made, the IBS rats were divided into IBS, HPM group, and pinaverium bromide (PB) group. The HPM received HPM for 20 min every day, while the PB was given gastric perfusion once a day for 14 days. After modeling and treatment, the abdominal withdrawal reflex, fecal character score, and fecal water content of rats were scored, and a 16S rRNA sequencing analysis was performed on the gut microbiota. Results: After treatment, the fecal character score and fecal water content in the HPM increased significantly, while visceral sensitivity decreased. Investigation of 16S rDNA sequencing revealed that α-diversity was reduced in the IBS, and HPM could increase the diversity of flora. The flora structure of IBS-D rats changed. HPM can increase the abundance of probiotics such as Akkermansia and reduce the abundance of opportunistic pathogens such as Bacteroides and Prevotella. Functional prediction analysis showed that the HPM was mainly related to the bacillary secret system, tricarboxylic acid cycle, and other pathways. Conclusion: HPM can regulate the gut microbiota of rats with IBS-D.","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":"9 1","pages":"141 - 149"},"PeriodicalIF":4.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45505025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using network pharmacology and molecular docking tools to investigate the potential mechanism of ephedra-gypsum in the treatment of respiratory diseases","authors":"Can Huang, Ling Yuan, Yang Niu, Yating Yang, Yi-Fan Yang, Yi Nan, Hong-Li Dou, J. Japhet","doi":"10.4103/2311-8571.378172","DOIUrl":"https://doi.org/10.4103/2311-8571.378172","url":null,"abstract":"Objective: The objective of this study was to investigate the potential mechanisms of ephedra-gypsum in the treatment of respiratory diseases (RDs) using network pharmacology and molecular docking techniques. Materials and Methods: The TCMSP and UniProt databases were used to mine the active components and targets of ephedra-gypsum, and the targets of RD were screened using the Online Mendelian Inheritance in Man (OMIM) and GeneCards databases. The protein-protein interaction network graph was created using the drug-disease intersection targets in the STRING database. The network diagram was analyzed using Cytoscape 3.9.1's topology function. The gene ontology (GO) and KEGG enrichment analyses were performed using the DAVID platform. Molecular docking bioactivity validation of the main active components and core targets was performed using AutoDock and PyMOL software. Results: Twenty-four compounds were screened, and 113 drug-disease targets overlapped. In total, 358 biological processes, 67 molecular functions, 38 cellular components of GO, and 139 pathways were identified. Molecular docking analysis demonstrated the strong binding ability of tumor protein 53 (TP53)-luteolin. Conclusion: The core components of ephedra-gypsum, such as quercetin, luteolin, kaempferol, and CaSO4·2H2O, act on key targets, such as tumor necrosis factor (TNF), interleukin-6 (IL-6), TP53, and IL-1 β through cytokine-mediated signaling pathways, inflammatory responses, cell proliferation, and apoptosis. This could be useful for the treatment of RD.","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":"9 1","pages":"150 - 159"},"PeriodicalIF":4.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48659726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of electroacupuncture on NLRP3 inflammasome and morphology of uterine in rats with primary dysmenorrhea based on meridian acupoint viscera correlation theory","authors":"Shao-hua Wang, Hanyu Yuan, Juan Li, Sian Pan, Xiao Xue, Zenghui Yue, Yu Liu","doi":"10.4103/2311-8571.378174","DOIUrl":"https://doi.org/10.4103/2311-8571.378174","url":null,"abstract":"Objective: To explore the relationship between acupoints and the uterus in primary dysmenorrhea (PDM) and observe the effects of electroacupuncture (EA) on NLRP3 inflammasome and morphology of uterine tissues in rats. Materials and Methods: Rats were divided into four groups: Control, PDM, EA, and nonacupoint EA (sham) groups. Writhing reactions in the rats were carefully monitored. Prostaglandin E2 (PGE2) and prostaglandin F2α (PGF2α) levels in the rat serum were measured using the enzyme-linked immunosorbent assay. To assess the damage, the histomorphology of the rat uterus was examined using the optical and electron microscopy. Western blotting was performed to determine the nucleotide-binding oligomerization domain carboxy-terminal leucine-rich repeat domain and amino-terminal pyrin domain-containing protein 3 (NLRP3), apoptosis-associated speck-like protein containing a caspase recruitment domain(ASC), and cysteinyl aspartate specific proteinase-1(caspase-1), interleukin-1β (IL-1β), and IL-18. Results: Compared with the control group, the PDM group exhibited significant increases in both writhing times and scores, with a high pathological score of the uterine tissue. Serum PGF2α levels were significantly elevated, whereas PGE2 levels were significantly reduced. In addition, the expression levels of NLRP3, ASC, caspase-1, IL-1β, and IL-18 significantly increased (P < 0.001). The EA group showed opposite effects from the PDM group (all P < 0.05). The sham group exhibited significantly higher writhing time, serum PGF2α levels, and expression levels of NLRP3, ASC, caspase-1, IL-1β, and IL-18 than the EA group. In contrast, serum PGE2 levels in the sham group were significantly lower than those in the EA group (all P < 0.05). Conclusions: NLRP3 inflammasome activation in PDM triggers pathological inflammation. Sanyinjiao(SP6) and Guanyuan(CV4) had specific effects on PDM. EA of SP6 and CV4 may treat PDM by inhibiting inflammation and protecting the morphological structure of the uterus.","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":"9 1","pages":"123 - 130"},"PeriodicalIF":4.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46697669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isobaric tag for relative and absolute quantitation-based proteomics for investigating the effect of Guasha on lumbar disc herniation in rats","authors":"Min Yang, Guitao Xu","doi":"10.4103/2311-8571.370107","DOIUrl":"https://doi.org/10.4103/2311-8571.370107","url":null,"abstract":"Objective: The objective of the study is to examine the possible mechanism by which Guasha (scraping therapy) affects the expression profiles of proteins in a lumbar disc herniation (LDH) rat model using isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomics. Methods: Thirty-six rats were used in this study. LDH rats were subjected to noncompressive LDH surgeries. Rats were randomly divided into the model and Guasha groups. Guasha was applied on alternate days for a total of nine times (three courses). At the end of each course, six rats were randomly selected from each group and their blood samples were collected. iTRAQ labeling was used to examine the mechanism of action of Guasha against LDH. The molecular functions, cellular components, and biological processes were analyzed using gene ontology analysis. The Ingenuity Pathway Analysis database was used to identify canonical pathways involving these proteins. Results: Compared to the model group, 198, 182, and 170 proteins were identified as differentially expressed at the three respective Guasha treatment courses. Pathways, including focal adhesion kinase signaling, acute-phase response signaling, and the LXR/RXR activation pathway, were closely related to the effects of Guasha in LDH rats. Furthermore, Rac1, Orm1, and Hpx were validated by western blotting, and the results were consistent with the protein expression levels observed using the iTRAQ method. Conclusion: Guasha could not only regulate the pathological changes related to LDH, but also achieve therapeutic effects by stimulating physiological changes. Our results offer a better understanding of the effects of Guasha on LDH.","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":"9 1","pages":"160 - 166"},"PeriodicalIF":4.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43687673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discussion on relationship between meridians and viscera of decreased ovarian reserve from the perspective of “preventive treatment of disease” by acupuncture","authors":"Sian Pan, Zhimiao Murong, Yi-Lin Zhu, Jia Song, Xiao-rong Chang, Yu Liu, Zenghui Yue","doi":"10.4103/2311-8571.378173","DOIUrl":"https://doi.org/10.4103/2311-8571.378173","url":null,"abstract":"Based on extensive historical data, clinical experience, and modern scientific research, we discuss the relationship between meridians and viscera of decreased ovarian reserve (OR) from the perspective of “preventive treatment of disease” by acupuncture. It is believed that the endogenous protection mechanism of acupuncture in “preventive treatment of disease” lies in harmonizing Yin and Yang and dredging meridians; stimulating righteousness and rejecting the evil outside; preventing disease; co-cultivating body and spirit, and regulating emotions. The incidence of OR dysfunction is closely related to the uterus-related meridians and viscera. The mechanism of acupuncture treatment of OR dysfunction may be related to reproductive endocrine hormone levels, ovarian tissue morphology, immune system, granulosa cell apoptosis, and related signal transduction pathways. The clinical effect of acupuncture treatment of diminished OR is reflected in improving menstruation and ovulation; improving OR function, promoting pregnancy; improving local ovarian microenvironment; improving negative emotions, improving quality of life, and thus affecting pregnancy outcomes. This article summarizes the mechanism of action and clinical effect of acupuncture in the treatment of decreased OR function based on recent findings, analyzes the advantages and disadvantages of acupuncture in the treatment of disease, and provides the rationale for the clinical application of acupuncture in the treatment of decreased OR function.","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":"9 1","pages":"111 - 122"},"PeriodicalIF":4.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47900641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network Pharmacology and Molecular Docking Analysis of the Mechanisms of Combined Radix Bupleuri (Chai-Hu) and Radix Paeoniae Alba (Bai-Shao) Treatment in the Prevention and Treatment of Alzheimer’s Disease","authors":"Zhao-Han Huang, Yuan Fang, Qi Yu, Tong Wang","doi":"10.4103/2311-8571.372730","DOIUrl":"https://doi.org/10.4103/2311-8571.372730","url":null,"abstract":"Abstract Objective: Radix Bupleuri and Radix Paeoniae Alba are referred to as Chai-Hu and Bai-Shao, respectively, in Chinese. We used molecular docking and network pharmacological approaches to explore the active components of this Chinese herb combination which is commonly used for treating Alzheimer’s disease (AD), the Chai-Hu and Bai-Shao pair (CBP). Materials and Methods: The active compounds and action targets of Chai-Hu and Bai-Shao were identified in the Traditional Chinese Medicine Systems Pharmacology database, and the Therapeutic Target Database, DrugBank, GenCards, DisGeNET, and Online Mendelian Inheritance in Man databases were used to identify therapeutic targets in patients with AD. To identify a common target of CBP in the treatment of AD, we used the Metascape tool to perform Gene Ontology function enrichment and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. In addition, CBP-AD protein–protein interaction (PPI) and herb-component-target networks were created using Cytoscape 3.8.2. Molecular docking was verified using AutoDock tools. Results: We identified 20 active compounds of CBP, 202 active targets, 1,323 disease targets, and 117 active compound disease intersection targets; the PPI network showed that the top eight key targets were AKT1, interleukin (IL) 6, JUN, MAPK1, tumor necrosis factor, TP53, vascular endothelial growth factor, and epidermal growth factor. These act mainly on the fluid shear stress, atherosclerosis, IL-17, hypoxia-inducible factor-1, and nonalcoholic fatty liver disease pathways, among others, which control immune inflammation, cell proliferation, and apoptosis. Based on molecular docking results, the active components of CBP bind well to its key targets. Conclusion: Our results indicated that CBP was effective for treating AD by reducing the expression of inflammatory factors, promoting cell proliferation and differentiation, and preventing nerve cell death and anti-neuronal cell apoptosis.","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134952758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Inflammatory Effect of Dendrobium huoshanense Polysaccharides on Carrageenan-Induced Air Pouch Synovitis in Mice","authors":"Hai-Jun Xu, Yang Yang, Ya-Qi Dong, Kai-Min Niu, Tian-Yu Li, Hui Deng, Jing-Wen Hao, Wassie Teketay","doi":"10.4103/2311-8571.372728","DOIUrl":"https://doi.org/10.4103/2311-8571.372728","url":null,"abstract":"Abstract Objective: The purpose was to observe the anti-inflammatory effects of Dendrobium huoshanense polysaccharides (DHPs) on carrageenan-induced sterile air pouch synovitis. Materials and Methods: A total of 30 Institute of Cancer Research (ICR) mice were randomly and equally assigned to the control, carrageenan-induced air pouch synovitis model (model), and carrageenan-induced air pouch synovitis model + DHP (200 mg/kg of body weight) (model + DHP) groups. Mice in the model + DHP group were intragastrically administered 200 mg/kg BW of DHP solution daily for 10 days. Mice in the control and model groups were intragastrically administered the same amount of distilled water. Two hours after intragastric administration on day 10, 1 mL of a 1% carrageenan solution in a sterile 0.9% saline solution was injected into the air pouch of mice in the model and model + DHP groups. Six hours later, the mice were sacrificed and 4 mL of ice-cold sterile 0.9% saline solution was injected into the air pouch to fully wash its inner wall. The lavage fluid was collected to observe the color and turbidity of the lavage fluid, as well as the appearance of the backside of the air pouch. The exudate volume, total number of leukocytes, protein content, levels of malondialdehyde (MDA), interleukin-1β (IL-1β), and tumor necrosis factor-alpha (TNF-α), and total superoxide dismutase (T-SOD) activity of the lavage fluid were analyzed. Results: The results showed that pretreatment with DHPs reduced the carrageenan-induced exudate volume ( P < 0.01), total leukocytes ( P < 0.05), and protein content ( P < 0.01) in the air pouch lavage fluid. Furthermore, mice in the model + DHP group had significantly higher ( P < 0.01) T-SOD activity and lower MDA content ( P < 0.05), IL-1β ( P < 0.05), and TNF-α ( P < 0.01) levels in the air pouch lavage fluid compared with the model group. Conclusion: It is concluded that DHPs partially alleviated carrageenan-induced sterile inflammation, and its mechanism may be related to reducing exudation and scavenging oxygen-free radicals, inhibiting lipid peroxidation, and reducing the level of the proinflammatory factors, such as IL-1β and TNF-α.","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":"365 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134952759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}