World Journal of Traditional Chinese Medicine最新文献

{"title":"Rapid Discovery, Guided Isolation, and Mechanism Study of Anti-neuroinflammatory Constituents from Glycosmis craibii var. glabra Targeting 5-LOX","authors":"Hongwei Chen, M. Ding, Shizhong Chen, Wenfu Tan, Y. S. Lin, Shuo Yuan, Li Li, Xinnuo Tong, Kewu Zeng, Pengfei Tu, Yong Jiang","doi":"10.4103/wjtcm.wjtcm_23_25","DOIUrl":"https://doi.org/10.4103/wjtcm.wjtcm_23_25","url":null,"abstract":"Abstract Objective: In this study, we aimed to screen and identify potent 5-lipoxygenase (5-LOX) inhibitors in Glycosmis craibii var. glabra extract and elucidate the mechanisms underlying their inhibitory effects. Materials and Methods: A 95% ethanol extract of G. craibii var. glabra was analyzed using an online ultra-performance liquid chromatography–photodiode array–5-LOX–fluorescence detector (UPLC–PDA–5-LOX–FLD) system. Guided by liquid chromatography–diode array detection/mass spectrometry (LC-DAD/MS) analysis, major peaks were isolated using various chromatographic techniques, and their structures were determined using high-resolution electrospray ionization mass spectrometry (HRESIMS), one-dimensional/two-dimensional nuclear magnetic resonance (1D/2D NMR), and electronic circular dichroism (ECD) data. Molecular docking, surface plasmon resonance (SPR) assays, and enzyme activity assays were performed to evaluate the binding capacity and inhibitory effects of the isolated compounds on 5-LOX. The anti-neuroinflammatory activities and mechanisms of action of these compounds were further examined in lipopolysaccharide (LPS)-stimulated BV-2 cells. Results: Eight main peaks were detected using the online UPLC–PDA–5-LOX–FLD system: one coumarin ( 1 ), one quinoline ( 2 ), three quinolones ( 3, 5, 8 ), and three flavonoids ( 4, 6, 7 ). Among these, compounds 4 and 5 were newly characterized, while compounds 5 and 6 exhibited the strongest binding affinity for 5-LOX. Mechanistic investigations revealed that compounds 5 and 6 suppressed TNF-α and IL-6 expression in LPS-stimulated BV-2 cells in a concentration-dependent manner by modulating the 5-LOX/CysLT 1 R pathway. Conclusions: This study successfully employed an online UPLC–PDA–5-LOX–FLD system to identify two major anti-inflammatory components of G. craibii var. glabra that target 5-LOX and exert their effects by regulating the 5-LOX/CysLT 1 R pathway.","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147381646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kaempferol Inhibits Inflammation via Regulating the Pi3k/Akt/Nuclear Factor Kappa B Pathway and Promotes Autophagy by Regulating the Pi3k/Akt/Mammalian Target of Rapamycin Pathway to Improve Podocyte Injury Induced By Antibody in Lupus Nephritis","authors":"Jian-bo Song, Liqin Zhao, Yuan-Ping Li, Hong-ping Wen","doi":"10.4103/wjtcm.wjtcm_48_25","DOIUrl":"https://doi.org/10.4103/wjtcm.wjtcm_48_25","url":null,"abstract":"Abstract Objective: This study aimed to explore the protective effects of kaempferol against podocyte injury in lupus nephritis (LN) and its underlying mechanism. Materials and Methods: Human podocytes were co-cultured with immunoglobulin G (IgG) from patients with LN (IgG-LN) to simulate podocyte injury in LN. Levels of oxidative stress and inflammatory factors were measured using the different kits. The expression of podocyte injury marker proteins was detected using immunofluorescence and western blotting. Then, we explored the interactions of kaempferol with PI3K, Akt, and nuclear factor kappa B (NF-κB) by molecular docking. Furthermore, PI3K agonists (740 Y-P) and inhibitors (LY294002) were used to explore the mechanism by which kaempferol alleviates IgG-LN-induced podocyte injury. Autophagy was measured by western blotting and Monodansylcadaverine staining. Results: The results indicated that kaempferol could protect podocyte injury induced by IgG-LN in a dose-dependent manner. Kaempferol significantly reduced oxidative stress, increased the activity of antioxidant enzymes, and reduced the levels of inflammatory factors in IgG-LN-treated podocytes. Kaempferol could bind to the active sites of PI3K, Akt, and NF-κB with high affinity, especially to NF-κB. LY294002 enhanced the ameliorative effect of kaempferol against IgG-LN-induced podocyte injury, whereas 740 Y-P weakened this protective effect. Compared to the model group, kaempferol significantly reduced the p-mTOR/mammalian target of rapamycin (mTOR) ratio and activated autophagy. Conclusions: Kaempferol can inhibit inflammation and oxidative stress through the regulation of PI3K/Akt/NF-κB pathway and promote autophagy by regulating the PI3K/Akt/mTOR pathway to improve IgG-LN induced podocyte injury.","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147907531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network Pharmacology and Experimental Validation-based Approach to Explore the Effect and Mechanism of Scutellaria baicalensis-Rhodiola rosea Herbal Pair against Ischemic Stroke","authors":"Shuxian Yang, Shusen Liu, Chen-xiao ZHI, Biao Wei, Jinjun Cheng, Zhigang Zhao, Ming-Hua Bai, B. Wang, Bin Zhu","doi":"10.4103/wjtcm.wjtcm_55_25","DOIUrl":"https://doi.org/10.4103/wjtcm.wjtcm_55_25","url":null,"abstract":"Abstract Objective: An integrated approach combining network pharmacology and experimental verification was used to elucidate the effects and mechanisms of the Scutellaria baicalensis (Huangqin [HQ]) and Rhodiola rosea (Hongjingtian [HJT]) herbal pair in treating ischemic stroke (IS). Materials and Methods: A compound–disease network for the HQ-HJT herbal pair and IS was constructed for the network pharmacology analysis. In addition, the efficacy and targets of the HQ-HJT herbal pair were verified using a classical in vitro IS model and molecular docking methods. Results: A total of 82 overlapping targets were identified between the HQ-HJT herbal pair and IS. Kaempferol was the most active compound in the compound–disease network. The protein-protein interaction network analysis showed that Interleukin-6, tumor necrosis factor-α (TNF-α), and HIF-1α were core targets. Kyoto Encyclopedia of Genes and Genomes analysis identified that the HQ-HJT herbal pair exerts therapeutic effects on IS through the TNF signaling and other related pathways. Our experimental analyses confirmed that the key components (kaempferol, salidroside, and baicalein) had neuroprotective effects in the oxygen-glucose deprivation/reoxygenation injury model. Notably, kaempferol suppressed inflammatory responses and showed strong binding affinity to six key targets within the HIF-1α/NLRP3/Caspase-1 signaling axis. Conclusions: HQ-HJT may exert an anti-IS effect by inhibiting the inflammatory cascade. Kaempferol may serve as a key bioactive compound in the HQ-HJT pair.","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147922858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Qingfei Liyan Decoction Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Synergistically Inhibiting the Cyclic GMP-AMP Synthase-Stimulator of Interferon Gene Pathway and Inflammasomes","authors":"Si-Min Chen, Qing Yao, Zhijie Ma, Ruilin Wang, Yan Wang, Xin-Ru Wen, Xianling Wang, Chengwei Li, Cui-hong Zheng, Xiaohe Xiao, Xiaoyan Zhan, Zhaofang Bai","doi":"10.4103/wjtcm.wjtcm_40_25","DOIUrl":"https://doi.org/10.4103/wjtcm.wjtcm_40_25","url":null,"abstract":"Objective: Acute lung injury (ALI) is a life-threatening respiratory disorder and a major concern in modern medical research. Clinical research has shown that Qingfei Liyan Decoction (QFLYT), a formula based on Yin Qiao San, is used to treat lung-associated inflammatory diseases. However, the mechanism through which QFLYT exerts its therapeutic effects remains unclear. The purpose of this study was to evaluate the effects of QFLYT on lipopolysaccharide (LPS)-induced ALI and investigate the underlying molecular mechanisms. Materials and Methods: We established an ALI model by administering LPS drops into the trachea of mice. Bronchoalveolar lavage fluid (BALF) and lung tissue were collected to determine inflammatory indices. The effects of QFLYT and its single herbs on the cyclic GMP-AMP synthase-stimulator of interferon gene (cGAS-STING) pathway and inflammasomes were studied in vitro . Relevant proteins and factors were detected using the enzyme linked immunosorbent assay, real-time quantitative polymerase chain reaction, and Western blotting. Results: QFLYT reduced the secretion of inflammatory factors in BALF and lung tissue, and attenuated pathological damage to the latter. In addition, QFLYT inhibited multiple stimuli-induced cGAS-STING pathway and inflammasomes activation in vitro . Further investigation revealed that the herbs in QFLYT had no combined effects on the activation of the two pathways at their formulated doses. However, Forsythiae fructus, Lonicerae japonicae flos, and Licorice inhibited the activation of both pathways at higher concentrations. Conclusions: Our findings show that QFLYT ameliorates LPS-induced ALI by inhibiting the cGAS-STING pathway and inflammasomes.","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":"11 4","pages":"488-503"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147886077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating the Anti-Atherosclerotic Mechanisms of Sanzi San: An Integrated Approach Using Serum Pharmacochemistry, Network Pharmacology, and Pharmacokinetics","authors":"Huiwen Zhang, Jianye Wang, Yanyan Liu, Jing-Min Tian, Jinbong Park, Huanyun Wang, Yingqi Xu","doi":"10.4103/wjtcm.wjtcm_49_25","DOIUrl":"https://doi.org/10.4103/wjtcm.wjtcm_49_25","url":null,"abstract":"Abstract Background: Atherosclerosis (AS) serves as the primary pathological foundation for numerous cardiovascular diseases, such as thromboembolic conditions, coronary artery disease, and ischemic cardiac-cerebral vascular disorders. Sanzi San, a traditional Chinese medicinal formulation with a long-standing clinical reputation for efficacy against AS, has not yet had its pharmacological mechanisms fully elucidated. Objective: Our objective is to investigate the impact of Sanzi San on AS in ApoE −/− mice models and elucidate the pharmacological mechanism via an integrated approach of serum pharmacochemistry, network pharmacology, and pharmacokinetic analysis. Materials and Methods: The chemical constituents in Sanzi San and serum were identified using high-performance liquid chromatography-quadrupole/Orbitrap high-resolution mass spectrometry (HPLC-Q/Orbitrap HRMS). A network pharmacology approach, including protein–protein interaction (PPI) and gene ontology enrichment analyses, was then used to investigate the connection between these components and their anti-atherosclerotic activity. Finally, ultra-HPLC-tandem mass spectrometry was used to determine the formula’s pharmacokinetic parameters following oral administration. Results: Through a comparison of serum samples from control and treated groups, 41 prototype compounds originating from Sanzi San were identified. These constituents were classified into three major groups – terpenes, iridoids, and phenolic acids – and were regarded as the potential bioactive components due to their absorption into the bloodstream. Network pharmacology analysis indicated that Sanzi San directly influences blood vessels by regulating their diameter to achieve therapeutic effects. Furthermore, Sanzi San demonstrated therapeutic potential by specifically targeting natural killer (NK) cells within plaque tissue and adventitial cells in the aorta. In vivo validation in ApoE −/− mice showed that Sanzi San improved aortic lesions and regulated body weight. Pharmacokinetic studies also revealed that seven major components of Sanzi San were present in plasma, confirming its pharmacological effects on AS. Conclusion: This study provides evidence of Sanzi San’s clinical efficacy in treating AS. The integrated strategy combining serum pharmacochemistry, network pharmacology, and pharmacokinetics offers a powerful approach for uncovering the key pharmacological mechanisms of herbal formulations.","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yinchen Wuling Powder Exerts Therapeutic Effects on Jaundice by Regulating Mitochondrial Biogenesis via the AMP-activated Protein kinase/Peroxisome Proliferator-activated Receptor Gamma Coactivator 1-alpha/Transcription Factor A, Mitochondrial Signaling Pathway","authors":"Yingrong Lao, Binglian Zhong, Chunyu He, Yang Liu, Xuemei Yang, Tiantian Hu, A Li, Weiguang Chen, Qin Liu, Hai-Tao Sun, Bin Wen, Weicong Chen, Songqi He","doi":"10.4103/wjtcm.wjtcm_36_25","DOIUrl":"https://doi.org/10.4103/wjtcm.wjtcm_36_25","url":null,"abstract":"Abstract Objective: Jaundice, a prevalent clinical condition associated with liver dysfunction, is characterized by elevated bilirubin levels and can lead to severe complications if untreated. Traditional Chinese medicine (TCM) has been utilized in the treatment of jaundice for centuries, with Yinchen Wuling powder (YCWLP) being one of the most notable herbal formulations. This study aimed to investigate the therapeutic effects of YCWLP in jaundice, focusing on its regulation of mitochondrial energy metabolism via the AMP-activated protein kinase (AMPK)/peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α)/transcription factor A, mitochondrial (TFAM) signaling pathway. Materials and Methods: An α-naphthyl isothiocyanate (ANIT)-induced jaundice mouse model was established. The mice were subsequently administered YCWLP orally. The impact of YCWLP on bilirubin metabolism was assessed by analyzing the levels of serum biochemical markers. Moreover, transmission electron microscopy was employed to observe mitochondria morphology. Nontargeted metabolomics of liver tissues, network pharmacology, quantitative real-time polymerase chain reaction, and western blot analyses were conducted on the molecular targets of YCWLP. In addition, a hyperbilirubinemia cell model was established using bilirubin/probenecid-treated HepG2 cells. Finally, these model cells were treated with YCWLP to further evaluate its regulatory effect on bilirubin metabolism. Results: Network pharmacology and metabolomics analyses indicated that the AMPK/PGC-1α/TFAM pathway’s plays a critical role in the onset and progression of jaundice by modulating mitochondrial function and energy metabolism. YCWLP administration significantly activated AMPK, which sequentially upregulated the expression of PGC-1α and TFAM. These alterations were associated with reduced reactive oxygen species production, restoration of mitochondrial membrane potential, as well as a significant decrease in intracellular total and indirect bilirubin levels. Finally, YCWLP exerted anti-jaundice effects through the enhancement of mitochondrial energy metabolism, mediated by the activation of the AMPK/PGC-1α/TFAM signaling pathway. This mechanism not only lowered bilirubin levels but also improved overall hepatic function. Conclusions: Our findings collectively highlight the potential of YCWLP as a therapeutic agent for the treatment of jaundice and related hepatic disorders, providing a scientific reference for its use in TCM.","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147905151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Huangqi Guizhi Wuwu Decoction Alleviates Oxaliplatin-Induced Peripheral Neuropathy by Reducing the Abundance of Akkermansia Muciniphila","authors":"Xi Chen, Zhengwei Zhang, Y Wang, Peng Cao, Zhen Zhu, Yang Yang","doi":"10.4103/wjtcm.wjtcm_97_24","DOIUrl":"https://doi.org/10.4103/wjtcm.wjtcm_97_24","url":null,"abstract":"Objective: Oxaliplatin-induced peripheral neuropathy (OIPN) limits chemotherapy doses and impacts the patient quality of life, with no available effective preventions or treatments. The Huangqi Guizhi Wuwu decoction (HGWD), a traditional Chinese medicine remedy, has been used to treat OIPN. Previous research showed that HGWD eases OIPN symptoms by regulating gut bacteria, but the exact bacteria involved remain unknown. Methods: We used a mouse model of OIPN to study HGWD’s effects. Fresh mouse fecal samples were 16S rRNA sequencing to identify the key bacteria. Akkermansia muciniphila (AKK) was introduced into the OIPN model before HGWD administration. We evaluated mechanical allodynia, cold hyperalgesia, and intestinal mucosa integrity at set intervals. A pseudo-sterile murine model was established through antibiotic intervention, followed by the transplantation of AKK. This model was utilized to assess the effects of HGWD on nociception and the integrity of the intestinal mucosa. Results: 16S rRNA sequencing showed that oxaliplatin treatment raised AKK levels, while HGWD treatment lowered them, reducing OIPN symptoms. An OIPN model with AKK transplantation revealed that higher AKK levels counteracted HGWD’s benefits, highlighting the importance of regulating AKK levels. Histopathological analysis revealed that HGWD enhanced intestinal mucosal health by decreasing AKK levels. Moreover, antibiotic intervention experiments demonstrated that HGWD mitigated OIPN by reducing the AKK levels and ameliorating intestinal mucosal damage. Conclusions: HGWD mitigates OIPN by decreasing the abundance of AKK and ameliorating its effects on intestinal mucosa.","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":"11 3","pages":"351-361"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147333339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anoectochilus Roxburghii Extract Ameliorates Acute Lung Injury in Mice by Suppressing the Cyclic-GMP-AMP Synthase Stimulator of Interferon Genes Pathway","authors":"Rui-Qin Ren, Yulu Yan, Chun-Qing Huang, Jun Wu, Cong-Qi Shen, Pingping Zheng, Y. -H. Liu, Ting-Ming Shen","doi":"10.4103/wjtcm.wjtcm_11_25","DOIUrl":"https://doi.org/10.4103/wjtcm.wjtcm_11_25","url":null,"abstract":"Background: Acute lung injury (ALI) is a severe respiratory disorder characterized by systemic immune dysregulation, where hyperactivation of the cGAS-STING pathway contributes to disease progression. Traditional Chinese medicine (TCM) represents a rich source of bioactive compounds that may offer novel therapeutic strategies for targeting this pathway. Objective: This study aimed to evaluate the potential of Anoectochilus roxburghii extract (ARE) as an inhibitor of the cGAS-STING pathway and assess its efficacy in alleviating ALI in experimental models. Methods: The inhibitory effects of ARE on cGAS-STING signaling were examined in bone marrow-derived macrophages (BMDMs) and THP-1 cells. An LPS-induced ALI mouse model was employed to investigate the anti-inflammatory properties of ARE. The therapeutic outcomes of high-dose ARE were compared with those of the STING inhibitor C-176. Results: ARE effectively suppressed cGAS-STING pathway activation without disrupting STING oligomerization. In the ALI model, ARE significantly mitigated lung inflammation, demonstrating comparable efficacy to C-176. Conclusion: These findings suggest that ARE is a promising natural candidate for treating ALI by targeting the cGAS-STING pathway. Its therapeutic potential may extend to other inflammatory diseases associated with cGAS-STING dysregulation.","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":"11 3","pages":"342-350"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147888505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Classification and Identification of Chemical Components of Biejiajian Pill Using Ultra-High-Performanc Liquid Chromatography Coupled with Hybrid Quadrupole-Orbitrap Mass Spectrometry","authors":"Bing-Zhao Du, Rong Zhu, Bo-Han Niu, Jiayao Yuan, Qian Bi, Shuxun Yan, Mingyi Shao, Yu Fu","doi":"10.4103/wjtcm.wjtcm_18_25","DOIUrl":"https://doi.org/10.4103/wjtcm.wjtcm_18_25","url":null,"abstract":"Objective: The objective of this study was to establish a systematic method for rapidly classifying and recognizing the chemical constituents of Biejiajian pill (BJJP) for safety in clinical applications. Materials and Methods: Ultra-high-performance liquid chromatography coupled with hybrid quadrupole-Orbitrap mass spectrometry (MS) was used for chemical profiling and identification of BJJP components. The chromatographic effluents were detected by MS in positive and negative ion modes. Results: A total of 217 compounds were structurally identified by deducing the mass fragmentation patterns and comparing with reference compounds and data reported in the literature, including 84 flavonoids, 49 phenolic constituents, 30 terpenoids, 24 triterpenoids, 7 alkaloids, 6 anthraquinones, and 17 other compounds. Conclusions: The established method can rapidly identify and analyze the chemical components of BJJP, laying the foundation for further analysis of active compounds, quality control, and metabolite fingerprinting.","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":"11 4","pages":"592-609"},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147880070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An In vivo Metabolic Reaction-Based Mass Difference Screening Strategy for Comprehensive Metabolite Profiling of the Huangqi-Danshen Herb Pair Using Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole-Time-of-Flight Mass Spectrometry","authors":"Chenxi Liu, Xinlu Li, Chaoyong Ma, Meng Ding, Ping Li, Hua Yang","doi":"10.4103/wjtcm.wjtcm_4_25","DOIUrl":"https://doi.org/10.4103/wjtcm.wjtcm_4_25","url":null,"abstract":"Objective: The absorbed prototypes and metabolites of traditional Chinese medicines in vivo are crucial for identifying their biological functions. However, comprehensive profiling of constituents in vivo is difficult owing to endogenous interference and low metabolite abundance. Therefore, a systematic strategy was proposed for screening and profiling exogenous components in biological matrices based on the mass differences between prototypes and the products of their metabolic reactions. Materials and Methods: Two hundred and forty-seven previously reported metabolic reactions were collected from the relevant literature on the Huangqi-Danshen (HD) herb pair, and a metabolic reaction-based mass difference database was created. The preprocessed data from experimental MS 1 based on ultra-high-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry were automatically calculated mass differences by R programming and matched with the database to screen potential constituents. Finally, the components were annotated across the MS 2 data. Results: A total of 164 components were annotated in the plasma samples from the rats after oral administration of HD, of which 20 were confirmed using the reference standards. The major metabolic pathways include methylation, dehydration, cysteine S-binding, glucuronidation, sulfation, and multistep reactions. Conclusions: This study revealed the metabolic characteristics and biotransformation regulation of HD and laid the foundation for elucidating its therapeutic effects. The proposed approach provides advantageous resources for evaluating the metabolites in the complex systems.","PeriodicalId":23692,"journal":{"name":"World Journal of Traditional Chinese Medicine","volume":"11 2","pages":"202-218"},"PeriodicalIF":0.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147879795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}