Wellcome Open Research最新文献

{"title":"Recruitment strategies used to enrol healthy volunteers in the first pneumococcal human infection study in Africa: Lessons from Blantyre, Malawi.","authors":"Edna Nsomba, Anthony E Chirwa, Clara Ngoliwa, Vitumbiko Nkhoma, Pemphero Liwonde, Edward Mangani, Modesta Reuben, Lorensio Chimgoneko, Lumbani Makhaza, Evaristar Kudowa, Marc Y R Henrion, Neema Toto, Stephen B Gordon, Dingase Dula","doi":"10.12688/wellcomeopenres.19271.2","DOIUrl":"10.12688/wellcomeopenres.19271.2","url":null,"abstract":"<p><strong>Background: </strong>Human Infection Studies (HIS) also known as Controlled Human Infection Models (CHIM) are a relatively new concept in African countries to clinicians, scientists, and communities alike. We have introduced HIS/CHIM studies to Malawi during the last four years by developing an experimental human pneumococcal carriage model. This CHIM was used to test the efficacy of a licensed 13-valent Pneumococcal Conjugate Vaccine (PCV13) against experimental nasal pneumococcal carriage. Traditional and digital recruitment strategies into this novel trial were explored.</p><p><strong>Objectives: </strong>To describe various methods of recruitment in this first CHIM study in Malawi.</p><p><strong>Methods: </strong>The clinical trial within the context of which these data were recorded was registered with the Pan African Clinical Trials Registry (REF: PACTR202008503507113) on 03 August 2020. The project was conducted at the Malawi Liverpool Wellcome Programme (MLW) in Blantyre, Malawi between April 2021, and September 2022. Source populations were college students and community members within Blantyre. Recruitment strategies included sharing study information in written or visual form, community sensitization meetings, snowball contacts (word of mouth from previous volunteers), branded clothing and participating in radio and television programs.</p><p><strong>Results: </strong>299 volunteers attended screening clinic, of whom 278 were recruited. Sixty-six recruited volunteers (23.7%) were college students and 212 (76.3%) were from the community. Snowball word-of-mouth contacting was the most successful recruitment strategy, with 201 (72.3%) participants recruited using this method. 195 (70.1%) were men of whom 149 (76.4%) joined the study through snowballing.</p><p><strong>Conclusion or recommendation: </strong>Using a variety of recruitment strategies led to successful recruitment in this novel controlled human infection study. Most participants were recruited through snowballing.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"9 ","pages":"216"},"PeriodicalIF":0.0,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11971628/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the Dotted Footman moth, <i>Pelosia muscerda</i> (Hufnagel, 1767).","authors":"Chris Fletcher, David C Lees","doi":"10.12688/wellcomeopenres.23766.1","DOIUrl":"10.12688/wellcomeopenres.23766.1","url":null,"abstract":"<p><p>We present a genome assembly from a male <i>Pelosia muscerda</i> (Dotted Footman; Arthropoda; Insecta; Lepidoptera; Erebidae). The genome sequence has a total length of 500.49 megabases. Most of the assembly (99.98%) is scaffolded into 30 chromosomal pseudomolecules, including the Z sex chromosome. The mitochondrial genome has also been assembled and is 15.42 kilobases in length.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"106"},"PeriodicalIF":0.0,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897693/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A chromosomal reference genome sequence for the northern house mosquito, <i>Culex pipiens</i> form <i>pipiens</i>, Linnaeus, 1758.","authors":"Jenny C Hesson, Yuki Haba, Carolyn S McBride, Edel Sheerin, Thomas C Mathers, Michael Paulini, Damon-Lee B Pointon, James W Torrance, Cibin Sadasivan Baby, Jonathan M D Wood, Shane A McCarthy, Mara K N Lawniczak, Alex Makunin","doi":"10.12688/wellcomeopenres.23767.1","DOIUrl":"10.12688/wellcomeopenres.23767.1","url":null,"abstract":"<p><p>We present a genome assembly from an individual female <i>Culex pipiens</i> sensu stricto (the northern house mosquito; Arthropoda; Insecta; Diptera; Culicidae), from a wild population in Sweden. The genome sequence is 533 megabases in span. Most of the assembly is scaffolded into three chromosomal pseudomolecules. The complete mitochondrial genome was also assembled and is 15.6 kilobases in length.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"107"},"PeriodicalIF":0.0,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11923538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the Large Birch Bell moth, Large Birch Roller, <i>Epinotia brunnichana</i> (Linnaeus, 1767).","authors":"Douglas Boyes, Finley Hutchinson, Liam M Crowley","doi":"10.12688/wellcomeopenres.23749.1","DOIUrl":"10.12688/wellcomeopenres.23749.1","url":null,"abstract":"<p><p>We present a genome assembly from a female <i>Epinotia brunnichana</i> (Large Birch Bell, Large Birch Roller; Arthropoda; Insecta; Lepidoptera; Tortricidae). The genome sequence has a total length of 943.10 megabases. Most of the assembly (99.68%) is scaffolded into 29 chromosomal pseudomolecules, including the W and Z sex chromosomes. The mitochondrial genome has also been assembled and is 15.7 kilobases in length. Gene annotation of this assembly on Ensembl identified 12,003 protein-coding genes.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"101"},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11959263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the click beetle, <i>Ampedus sanguinolentus sanguinolentus</i> (Schrank, 1776).","authors":"Duncan Sivell, Dmitry Telnov, Michael F Geiser, Maxwell V L Barclay","doi":"10.12688/wellcomeopenres.23712.1","DOIUrl":"10.12688/wellcomeopenres.23712.1","url":null,"abstract":"<p><p>We present a genome assembly from a female specimen of <i>Ampedus sanguinolentus sanguinolentus</i> (click beetle; Arthropoda; Insecta; Coleoptera; Elateridae). The assembly contains two haplotypes with total lengths of 1,574.76 megabases and 1,572.87 megabases. Most of haplotype 1 (97.13%) is scaffolded into 10 chromosomal pseudomolecules, while haplotype 2 is a scaffold-level assembly. The mitochondrial genome has also been assembled and is 15.99 kilobases in length.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"96"},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Data note on the Avian Contact Study: a questionnaire resource for avian influenza public health planning.","authors":"Amy C Thomas, Suzanne Gokool, Genevieve Clapp, Harry Whitlow, Carmel McGrath, Peter Moore, Maud Helwegen, Mariam Logunleko, Irene Bueno, Mick Bailey, Sarah Masterton, Jo Taylor-Egbeyemi, Ian Brown, Richard Puleston, Riinu Pae, Louise E Smith, Sarah Lambton, Ellen Brooks-Pollock","doi":"10.12688/wellcomeopenres.23064.2","DOIUrl":"10.12688/wellcomeopenres.23064.2","url":null,"abstract":"<p><p>The Avian Contact Study is a cross-sectional questionnaire of poultry workers and other individuals who have contact with domestic and/or wild birds in the UK. The questionnaire was launched online in May 2024 and in-person responses were gathered at the British Pig and Poultry Fair in Birmingham on 15 and 16 May 2024. This Data Note documents questionnaire development, content and delivery methods. Data collected include information on demographics, seasonal influenza vaccination, avian influenza exposure, contact with birds and people, and awareness of avian influenza. This Data Note provides insights into the first data release collected up to 31 July 2024 for 225 respondents. Data have been released as a University of Bristol held dataset available upon request. The Avian Contact Study provides a pilot resource for research into avian influenza from a zoonotic perspective.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"9 ","pages":"604"},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acceptability trial of local Indonesian snack (SISTIK) enriched with chicken liver and eggshell powder as a potential food to increase micronutrient intakes among women of reproductive age.","authors":"Yenni Zuhairini, Aghnia Husnayiani Suryanto, Qorinah Estiningtyas Sakilah Adnani, Mohammad Brachim Anshari, Haidar Rizqi, Annisha Fathonah, Afini Dwi Purnamasari, Afiyah Hadiyanti Pangasih, Ayunda Jihadillah, Dina Novtyana Puspita, Dimas Erlangga Luftimas, Sofa Rahmannia, Umi Fahmida, Rosalind Gibson, Aly Diana","doi":"10.12688/wellcomeopenres.20292.2","DOIUrl":"https://doi.org/10.12688/wellcomeopenres.20292.2","url":null,"abstract":"<p><strong>Background: </strong>Chronic micronutrient deficiencies in Indonesian women and young children contribute to poor foetal and infant growth. To address this, we formulated micronutrient-enriched crackers (MEC) incorporating nutrient-rich chicken liver, rich in iron, zinc, vitamin A, and B vitamins, along with powdered eggshells as a calcium source. Known locally as ' <i>sistik</i>,' MEC may provide a sustainable solution to improve micronutrient intakes. This study evaluated MEC acceptability among non-pregnant women of reproductive age to ensure safety and feasibility before extending future research to pregnant and lactating populations.</p><p><strong>Methods: </strong>A single-blinded, two-phase acceptability trial involved 81 non-pregnant women aged 19-35 years in Ujung Berung Sub-district, Bandung City, Indonesia. In Phase 1, participants sampled both MEC and standard wheat crackers (SWC) in a test feeding session and rated each product using a 7-point cued facial response scale, evaluating colour, smell, flavour, and texture. In Phase 2, participants were randomly assigned to receive a 14-day home supply (75 g/day) of either MEC (n=41) or SWC (n=40). Adherence was determined by weighing unconsumed products. Data were analysed using mixed linear model for liking scores and a t-test for adherence.</p><p><strong>Results: </strong>MEC received favourable ratings, with no significant differences compared to SWC in liking scores for colour (MEC 4.5±1.2 vs. SWC 5.4±0.9), smell (MEC 4.9±1.5 vs. SWC 5.6±0.9), flavour (MEC 4.9±1.4 vs. SWC 5.6±0.9), and texture (MEC 5.4±1.0 vs. SWC 5.7±0.8); p-value > 0.05. Average daily consumption over 14 days was comparable between groups (MEC: 50.8±23.0 g vs. SWC: 51.0±20.0 g; mean difference: -0.2 g; 95% CI: -6.5 to 6.1; p-value = 0.802).</p><p><strong>Conclusions: </strong>MEC demonstrates reasonable acceptability and feasibility as a daily snack, supporting its potential as a dietary intervention to improve women's micronutrient intake and reduce infant stunting, especially among those women who found it appealing.</p><p><strong>Registration: </strong>ClinicalTrials.gov ( NCT04564222, 25 ^th September 2020).</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"9 ","pages":"550"},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the Tropical Bluetail Damselfly, <i>Ischnura senegalensis </i>(Rambur, 1842).","authors":"Beatriz Willink","doi":"10.12688/wellcomeopenres.23747.1","DOIUrl":"https://doi.org/10.12688/wellcomeopenres.23747.1","url":null,"abstract":"<p><p>We present a genome assembly from a specimen of <i>Ischnura senegalensis</i> (Tropical Bluetail; Arthropoda; Insecta; Odonata; Coenagrionidae). The assembly contains two haplotypes with total lengths of 1,599.82 megabases and 1,602.78 megabases. Most of haplotype 1 (96.41%) is scaffolded into 14 chromosomal pseudomolecules, including the X sex chromosome, which haplotype 2 is a scaffold-level assembly. The mitochondrial genome has also been assembled and is 18.11 kilobases in length.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"104"},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12033981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144041089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of an orbweaving spider, <i>Gibbaranea gibbosa</i> (Walckenaer, 1802).","authors":"Liam M Crowley, Craig S Wilding","doi":"10.12688/wellcomeopenres.23751.1","DOIUrl":"10.12688/wellcomeopenres.23751.1","url":null,"abstract":"<p><p>We present a genome assembly from a specimen of <i>Gibbaranea gibbosa</i> (orbweaving spider; Arthropoda; Arachnida; Araneae; Araneidae). The genome sequence has a total length of 2,816.88 megabases. Most of the assembly (98.61%) is scaffolded into 13 chromosomal pseudomolecules, including the X ₁ and X ₂ sex chromosomes. The mitochondrial genome has also been assembled and is 14.1 kilobases in length.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"97"},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11953616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the Coppice Mining Bee, <i>Andrena helvola</i> (Linnaeus, 1758).","authors":"Steven Falk, Joseph Monks","doi":"10.12688/wellcomeopenres.23746.1","DOIUrl":"10.12688/wellcomeopenres.23746.1","url":null,"abstract":"<p><p>We present a genome assembly from a female <i>Andrena helvola</i> (Coppice Mining Bee; Arthropoda; Insecta; Hymenoptera; Andrenidae). The genome sequence has a total length of 442.47 megabases. Most of the assembly (91.93%) is scaffolded into 7 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 16.61 kilobases in length.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"102"},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897692/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}