Voprosy virusologii最新文献

{"title":"Nef HIV-1 (<i>Retroviridae: Orthoretrovirinae: Lentivirus: Human immunodeficiency virus-1</i>), multifunctional protein: features of genetic virus variants circulating in Russia.","authors":"A I Kuznetsova, A A Antonova, L A Protasova, A V Glyakina, K V Kim, I M Munchak, E N Mezhenskaya, E A Orlova-Morozova, A Y Pronin, A G Prilipov, O V Galzitskaya","doi":"10.36233/0507-4088-305","DOIUrl":"10.36233/0507-4088-305","url":null,"abstract":"<p><strong>Introduction: </strong>Nef provides high level of HIV-1 replication due to synergy of its multiple functions and is an important factor in the pathogenesis of HIV infection. Nef is considered as a target for development of therapeutic agents. Mutations of drug resistance to dolutegravir can occur in Nef protein. Natural amino acid substitutions in Nef protein have been associated with the degree of progression of HIV infection, development of neurodegenerative and cardiovascular diseases in patients. The aim of the study is to investigate Nef genetic diversity in HIV-1 variants circulating in Russia and in Moscow region.</p><p><strong>Materials and methods: </strong>Total 216 Nef sequences obtained from whole blood samples of patients and 77 sequences downloaded from the Los Alamos International Database were analyzed. Consensus sequences of Nef sub-subtype A6, subtype B, CRF02_AG, CRF63_02A6, CRF133_A6B, and the reference sequence NL4-3 were compared. Genetic diversity of Nef sub-subtype A6 (Nef-A6) in patients with different stages of the disease was assessed. The presence of dolutegravir-associated drug resistance mutations in the Nef protein in HIV-1 variants circulating in Russia was also investigated.</p><p><strong>Results: </strong>Differences in the spatial structures in consensus sequences of the studied HIV-1 variants were determined. It was shown that the conservatism of Nef-A6 in groups of patients with later stages of the disease was significantly higher. No mutations of drug resistance to dolutegravir were detected.</p><p><strong>Conclusion: </strong>The differences in Nef sequences of HIV-1 variants circulating in Russia could affect the functional properties of the protein and could be taken into account in creating Nef-based therapies in the future. Obtained results indicate that there is no risk of resistance to dolutegravir associated with - mutations in the Nef protein. It outlines possible directions for further research into the genetic diversity of Nef.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"70 6","pages":"518-535"},"PeriodicalIF":0.0,"publicationDate":"2025-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146031002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The immune response after vaccination in recipients against different subtypes of tick-borne encephalitis virus (<i>Flaviviridae</i>: <i>Orthoflavivirus</i>).","authors":"E A Orlova, A L Ivanova, V A Mishchenko, I P Bykov, I V Vyalykh, N L Fadeeva, V V Patlusova, M F Vorovitch, N M Kolyasnikova","doi":"10.36233/0507-4088-348","DOIUrl":"10.36233/0507-4088-348","url":null,"abstract":"<p><strong>Introduction: </strong>There are three antigenic subtypes of tick-borne encephalitis virus (TBEV): the European, Far Eastern and Siberian subtypes. The article discusses the topic of cross-protective neutralizing antibodies against different subtypes of TBEV. Objective ‒ the study of the immune response after vaccination against TBE in recipients immunized with Russian-made vaccines in relation to Siberian, Far Eastern, and European subtypes.</p><p><strong>Materials and methods: </strong>100 serum samples obtained from recipients vaccinated against TBE. ELISA reagent kit was used to detect IgG antibodies to TBEV. The neutralization reaction on cell culture was used to analyze the titer of neutralizing antibodies. The following TBEV strains were used: Sofyin; Vasilchenko; Absettarov; Ekb_1887_1.</p><p><strong>Results: </strong>A decrease in the levels of neutralizing antibodies against heterologous strains compared to the vaccine strain was observed: for the Siberian strains Ekb_1887_1 and the Vasilchenko, a decrease was of 3.9 and 2.4 times, respectively; for the European strain, a 4.9-fold decrease compared to vaccine strain was observed. In case when IgG antibody titers were below 1 : 500, the titers of antibodies to TBEV strains heterologous to the vaccine did not exceed the minimum detectable value of 1 : 10. For individuals with IgG antibody titers below 1 : 100, antibodies to Sofyin strain were not detected. Individuals with reduced titers of virus-specific antibodies more often had deviations from the recommended vaccination schedule.</p><p><strong>Conclusion: </strong>Given the widespread distribution and genetic variability of the Siberian subtype, as well as the limited cross-neutralization capabilities of existing vaccines, the task of developing a combined vaccine that includes antigens of several virus subtypes seems relevant.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"70 6","pages":"559-569"},"PeriodicalIF":0.0,"publicationDate":"2025-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146031051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR-Cas genome editing system in the diagnosis and therapy of infection caused by herpes simplex virus type 1 (<i>Orthoherpesviridae: Alphaherpesviridae: Simplexvirus: Simplexvirus humanalpha1</i>).","authors":"N A Demidova, R R Klimova, A A Kushch, D S Karpov","doi":"10.36233/0507-4088-307","DOIUrl":"10.36233/0507-4088-307","url":null,"abstract":"<p><p>Herpes simplex virus type 1 (HSV-1), newly named as <i>Simplexvirus humanalpha1</i> is one of the most common pathogens in the human population, which can cause severe disease, often with fatal outcomes. Diagnostic methods currently in use are specific and sensitive, but time-consuming, require expensive laboratory equipment and highly qualified personnel. Existing therapeutic agents have a number of significant drawbacks. To successfully treat and prevent the spread of the infection, new rapid, easy-to-use, and highly sensitive diagnostic tools and effective therapeutic agents are required. One approach to achieve this goal is CRISPR-based technology. This review analyzes information obtained from a literature search in the Scopus, Web of Science and MedLine databases on the topics «HSV-1, structure, distribution, life cycle», «new methods for molecular diagnosis of HSV-1-infection», «classification of CRISPR-Cas systems», «nucleic acid amplification methods», «CRISPR-Cas effector proteins», «application of CRISPR-Cas systems in molecular diagnostics of HSV-1-infection», «application of CRISPR-Cas systems in therapy of HSV-1-infection». New approaches of CRISPR using effector proteins Cas12 and Cas13 in the diagnosis of HSV-1 infections are reviewed. The article discusses the progress in the development of CRISPR-Cas-based therapies against HSV-1-infection <i>in vitro</i> and <i>in vivo</i>. CRISPR gene therapy <i>in vivo</i> has a great clinical potential, but its safety and efficacy require further investigation. An analysis of the available data suggests that CRISPR-based technologies offer promising prospects for expanding the arsenal of diagnostic tools and antiviral drugs in the context of current and future outbreaks of viral diseases.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"70 6","pages":"493-507"},"PeriodicalIF":0.0,"publicationDate":"2025-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146030947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence of Anti-Mpox Virus IgG Antibody and Awareness of Mpox Disease in Ibadan, Southwest Nigeria.","authors":"J Olayiwola, A Akagbosu, O Samson, A Alaba, B Aponjolosun, S Soyemi","doi":"10.36233/0507-4088-337","DOIUrl":"https://doi.org/10.36233/0507-4088-337","url":null,"abstract":"<p><strong>Purpose: </strong>Mpox cases were previously common in children; recent outbreaks of clade II have mostly affected young adults. Therefore, this study examines the knowledge, attitudes, and seroprevalence of the Mpox virus among consenting participants in Ibadan.</p><p><strong>Materials and methods: </strong>Eligible individuals were those who voluntarily participated in the study and met the inclusion criteria specified for the study. A cross-sectional survey was conducted involving 94 respondents, investigating socio-demographic factors, awareness levels, attitudes toward prevention, and infection rates. The anti-Mpox virus IgG antibody was detected quantitatively using the Enzyme-Linked Immunosorbent Assay (ELISA) technique. The data were then analyzed using the χ² test, while the antibody quantification was displayed with a Box and Whisker plot; statistical significance was determined at <i>p</i> < 0.05.</p><p><strong>Results: </strong>The majority of respondents were female (66.7%) and aged 58 years and above (20.0%). Most had tertiary (40.0%) and secondary education (34.4%). Awareness of the Mpox was moderate, with 61.1% having heard of the virus, primarily through news (20.0%) and healthcare workers (18.9%). However, knowledge gaps were evident: only 38.9% recognized symptoms, and 40.0% understood modes of transmission. Attitudes towards prevention were generally positive; 60.0% believed Mpox could be prevented, and 73.3% were willing to take a vaccine. Still, readiness to engage in screening was low; 81.1% had never been tested, and 58.9% were unaware of local test availability. Regarding seroprevalence, females showed a significantly higher infection rate (27.4%) than males (9.6%) (χ² = 3.854, <i>p</i> = 0.050). Age-wise, the highest infection rates occurred in those < 18 years (61.5%) and 53-57 years (66.6%) (χ² = 30.817, <i>p</i> = 0.000), indicating significant age-related differences.</p><p><strong>Conclusion: </strong>The findings highlight the need for targeted public health education, increased test access, and focused intervention strategies to improve Mpox virus prevention and control, especially among vulnerable age groups and under-informed populations.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"70 5","pages":"463-470"},"PeriodicalIF":0.0,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145606401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Method for determining infectious and hemagglutinating titer of influenza A/Mallard Pennsylvania/10218/84 (H5N2) virus by changing the microviscosity of the viral membrane after interaction with phospholipid modifiers using cholesterol free liposomes as an example].","authors":"N A Kontarov, I V Pogarskaya, E I Dolgova, E O Kontarova, V V Pomazanov, R R Gafarov, S G Mardanly, N V Yuminova","doi":"10.36233/0507-4088-326","DOIUrl":"https://doi.org/10.36233/0507-4088-326","url":null,"abstract":"<p><strong>Introduction: </strong>The antiviral action of a number of drugs is associated with their modification of the lipid membrane of viruses. One of the possible mechanisms of such modification of the viral membrane is the extraction of cholesterol from the membranes of virions.</p><p><strong>Objective of the study: </strong>A method has been developed for determining the infectious and hemagglutinating titer of avian influenza virus by changing the microviscosity of the viral membrane after incubation with phospholipid modifiers, using cholesterol-free liposomes consisting of phosphatidylcholine and phosphatidylethanolamine in a molar ratio of 1 : 2 for 48 hours as an example.</p><p><strong>Materials and methods: </strong>The extraction process was confirmed by two methods: gel filtration with radioactively labeled liposomes and virions, and by changing the polarization value of the fluorescent probe 1-anilinonaphthalene-8-sulfonate anion (8-ANS) in the viral membrane.</p><p><strong>Results: </strong>A correlation was found between the change in infectious and hemagglutinating titer and the microviscosity of the viral membrane.</p><p><strong>Conclusion: </strong>In this regard, it seems possible to use this dependence to determine the infectious and hemagglutinating activity of the influenza virus within one serotype in clinical laboratory diagnostics, using various fluorescent probes. It should be noted that not only liposomes of a certain composition can be used as lipophilic modifiers of the viral membrane, but also such compounds as ethylene glycol, erythritol, glycerol.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"70 5","pages":"471-476"},"PeriodicalIF":0.0,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145606255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Antibodies against VP3 protein of echovirus 30 (<i>Picornaviridae: Enterovirus: Enterovirus betacoxsackie</i>) neutralize virus <i>in vitro</i>].","authors":"D V Novikov, D A Melentev, V Y Talayev, N A Novikova, E V Mokhonova, A U Kashnikov, I E Zaichenko, M V Svetlova, E V Kurkova, O N Babaykina, V A Lapin, M I Tsyganova, D E Zaitsev, V V Novikov","doi":"10.36233/0507-4088-347","DOIUrl":"https://doi.org/10.36233/0507-4088-347","url":null,"abstract":"<p><strong>Introduction: </strong>The widespread occurrence of enterovirus echovirus 30 (E30) and cases of severe disease indicate the need for vaccine development. Epitopes for neutralizing antibodies and T-cell response have been found in VP3 proteins of some enteroviruses. However, the immunogenic properties of VP3 E30 have not been studied. The aim of this work is to characterize the immunogenic properties of VP3 E30 and testing the virus-neutralizing properties of antibodies against VP3 E30.</p><p><strong>Materials and methods: </strong>VP3_E30 and the chimeric protein S_N-VP3_E30, consisting of the S region of norovirus VP1 and VP3 E30 were expressed in <i>Escherichia</i> <i>coli</i>. S_N-VP3_E30 was used to form virus-like particles (VLPs). The effect on human dendritic cells (DCs) was assessed by flow cytometry to measure changes in HLA-DR, CCR7, CD80, CD83 and CD86 expression. BALB/c mice and guinea pig were used for immunization with S_N-VP3_E30. Antibody titers and avidity were determined by ELISA. The interaction of antibodies with VP3 E30 was studied by immunoelectron microscopy and virus neutralization in embryonal rhabdomyosarcoma (RD) cell culture.</p><p><strong>Results: </strong>Recombinant VP3 E30 caused incomplete DCs maturation, characterized by the understimulation of the CCR7 chemokine receptor expression. Inclusion of VP3 in the chimeric VLPs resulted in complete DCs maturation and a strong humoral immune response in laboratory animals. Antibodies against VP3 were characterized by high avidity, the ability to induce agglomeration of viral particles and neutralization of E30 in RD cell culture.</p><p><strong>Conclusion: </strong>The obtained results indicate that VP3 can be used as an antigen in the composition of a subunit vaccine against enterovirus E30.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"70 5","pages":"477-486"},"PeriodicalIF":0.0,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145606185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatitis B and C viruses seroprevalence and risk factors among health care workers (HCWs) in referral hospitals in Brazzaville, Republic of Congo.","authors":"J Malanda-Kiminou, F Got, G Malonga","doi":"10.36233/0507-4088-334","DOIUrl":"https://doi.org/10.36233/0507-4088-334","url":null,"abstract":"<p><strong>Introduction: </strong>Hepatitis B and C viruses cause chronic infections leading to liver cirrhosis and hepatocellular carcinoma. This study aimed to determine the seroprevalence of hepatitis B and C viruses and the potential risk factors which might influence the prevalence among health care workers (HCWs) in Brazzaville, Republic of Congo.</p><p><strong>Materials and methods: </strong>We conducted a cross-sectional study from June to November 2022 among HCWs from the Talangaï and Makélékélé referral hospitals, Brazzaville. 107 HCWs were included and serological screening was carried out using rapid screening tests followed by the ELISA technique, after completing a survey questionnaire.</p><p><strong>Results: </strong>The mean age was 36.56 ± 11.62 years with a female predominance of 1 : 0.36 sex ratio, laboratory technicians were the most represented socio-professional category. Seroprevalence of HBV was 7.48% and that of HCV was 3.74%. During their service, 40% have already been the victims of blood exposure accidents and have 7 times more risk of contracting HBV (odd ratio [OR] = 7.01 (95% Confidence interval [CI] 1.54-31.96); <i>p</i> = 0.01).</p><p><strong>Conclusion: </strong>These data show that hepatitis B and C viruses are still endemic among HCWs in Republic of Congo. We can conclude that the health care sector is a high-risk profession due to infection with hepatitis B and C viruses. It is therefore necessary to improve the health and safety conditions of HCWs, implement new strategies to reduce occupational exposure to blood and body fluids, and reduce viral contamination by hepatitis B and C.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"70 5","pages":"455-462"},"PeriodicalIF":0.0,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145606450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virus taxonomy and megataxonomy (Vira domain) - current status.","authors":"D K Lvov, V G Akimkin, A D Zaberezhny, S V Borisevich, S V Alkhovsky","doi":"10.36233/0507-4088-344","DOIUrl":"https://doi.org/10.36233/0507-4088-344","url":null,"abstract":"<p><p>For nearly 80 years since the discovery of the first virus by the Russian scientist D.I. Ivanovsky, it has been recognized that all organisms of Earth's biosphere serve as natural hosts for viruses. Viruses, grouped within the informal domain <i>Vira</i>, infect all three domains of cellular life: archaea - <i>Archaea, </i>bacteria - <i>Bacteria</i>, and eukaryotes - <i>Eucarya</i> (algae, fungi, protozoa, plants, invertebrates, and vertebrates). The formation of viral population gene pools through interactions with the gene pools of their hosts has taken place under changing environmental conditions over 3.5 billion years, giving rise to the vast diversity of the virosphere. The accumulation of data on the Earth's virosphere, facilitated by the advent of high-throughput sequencing technologies (NGS), has necessitated a reassessment of approaches to virus classification and, since 2018, has led to a reform of viral taxonomy through the introduction of higher taxonomic ranks (megataxonomy). As of September 2025, the International Committee on Taxonomy of Viruses (ICTV) recognizes 15 taxonomic ranks for viruses, the most significant being: realm - 7, kingdom - 11, phylum - 23, class - 49, order - 93, family - 368, genus - 3769, and species - 16,215. Ongoing advances in metagenomics, metatranscriptomics, and the global ecology of the virosphere will inevitably drive further changes in viral taxonomy and megataxonomy. These developments are of fundamental importance for understanding the evolution of the biosphere and of practical relevance for developing new strategies to strengthen biological security and to mitigate the consequences of epidemic emergencies associated with emerging and reemerging infections.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"70 5","pages":"401-416"},"PeriodicalIF":0.0,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145606417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Resistance and chemosensitivity restoration in human cytomegalovirus-infected tumor cells to doxorubicin through combined treatment with aqueous fullerene dC₆₀].","authors":"Y Y Chernoryzh, K I Yurlov, T V Grebennikova, S M Andreev, E I Lesnova, R A Simonov, N E Fedorova, A A Kushch","doi":"10.36233/05074088300","DOIUrl":"https://doi.org/10.36233/05074088300","url":null,"abstract":"<p><strong>Introduction: </strong>Human cytomegalovirus infection can induce tumor cell resistance to chemotherapeutic agents through modulation of apoptotic pathways. In the search for alternative approaches to overcome virus-associated drug resistance, the application of nanomaterials (aqueous fullerene dC₆₀) represents a promising strategy. The potential to overcome human cytomegalovirus mediated chemoresistance opens new avenues for developing combined therapeutic approaches in oncology. Aim - to evaluate the impact of human cytomegalovirus infection on the resistance of hepatocellular carcinoma and promyelocytic leukemia cells to doxorubicin, as well as the potential of aqueous fullerene dC₆₀ to restore chemosensitivity in monocytic leukemia cells.</p><p><strong>Materials and methods: </strong>Hepatocellular carcinoma cells (Huh 7.5), promyelocytic leukemia cells (HL-60), monocytic leukemia cells (THP-1), and HCMV AD169 were used. The experimental procedures included standard cell culture techniques, virological methods, immunocytochemistry, Western blotting, Real-Time Polymerase Chain Reaction, Quantitative Reverse Transcription Polymerase Chain Reaction and MTT assay.</p><p><strong>Results: </strong>Human cytomegalovirus infection reduced doxorubicin cytotoxicity by 30% in both hepatocellular carcinoma and promyelocytic leukemia cells. In monocytic leukemia cells, combined treatment with doxorubicin and dC₆₀ restored chemosensitivity to human cytomegalovirus infected cells, achieving 93% tumor cell death at half the standard doxorubicin concentration.</p><p><strong>Conclusion: </strong>Human cytomegalovirus infection induces doxorubicin resistance in both hematopoietic (promyelocytic leukemia, monocytic leukemia) and solid (hepatocellular carcinoma) tumor models. Importantly, combined treatment doxorubicin with aqueous fullerene dC₆₀ not only overcomes virus-mediated drug resistance in monocytic leukemia cells but also enhances cytotoxicity at reduced doxorubicin concentrations, offering prospects for developing less toxic combined therapeutic regimens.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"70 5","pages":"444-454"},"PeriodicalIF":0.0,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145606297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NGS amplification panel HCV-seq for sequencing hepatitis C virus RNA (<i>Flaviviridae</i>: <i>Hepacivirus</i>).","authors":"M D Chanyshev, A S Chernyshova, A G Glushchenko, A A Grishaeva, V V Makashova, Z B Ponezheva, K F Khafizov, V G Akimkin","doi":"10.36233/0507-4088-331","DOIUrl":"https://doi.org/10.36233/0507-4088-331","url":null,"abstract":"<p><strong>Introduction: </strong>Hepatitis C is a pressing global public health issue. The high variability of the hepatitis C virus (HCV) complicates its whole-genome sequencing; most studies sequence only specific regions of the genome. There is a need for a simple and reliable method for sequencing the whole genome of HCV.</p><p><strong>Objective: </strong>Development and validation of NGS panel for whole-genome sequencing of HCV.</p><p><strong>Materials and methods: </strong>This study presents NGS amplification panel for sequencing the genomes of HCV genotypes 1, 2, and 3. Depending on the genotype, a set of 79, 67, or 89 primers is used. These primers enable amplification of overlapping regions of the HCV genome.</p><p><strong>Results: </strong>The panel was tested on 153 HCV RNA samples isolated from blood plasma specimens (93/6/54 samples of genotypes 1/2/3, respectively). Shannon entropy analysis showed that genetic heterogeneity within the <i>E2</i> gene is significantly higher than in other parts of viral genome. The frequency of mutations associated with drug resistance was determined. Specifically, for genotype 1, the following mutation detection rates were observed in NS3: Y56F - 37.6%, V170I - 23.7%; in NS5a: R30Q - 8.6%, P58L/S/T - 6.5%, A92T - 4.3%; in NS5b: L159F - 45.2%, S556G/N - 33.3%.</p><p><strong>Conclusion: </strong>The current study describes a method for whole-genome sequencing of HCV genotypes 1, 2, and 3. The HCV sequencing panel shows great potential for use in scientific research and epidemiological monitoring.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"70 4","pages":"363-373"},"PeriodicalIF":0.0,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145378966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}