Voprosy virusologii最新文献

{"title":"Lyudmila V. Kolobukhina.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"69 4","pages":"392"},"PeriodicalIF":0.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142373065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Review of the collective monograph edited by Academician of the Russian Academy of Sciences V.I. Zlobin «Tick-borne encephalitis in the 21st century».","authors":"O P Zhirnov","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"69 4","pages":"390-391"},"PeriodicalIF":0.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142373068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The effect of sodium deoxyribonucleate with iron complex on the expression of surface markers of MT-4 cells infected with human immunodeficiency virus type 1 (HIV-1) (Retroviridae: <i>Primate lentivirus group</i>)].","authors":"D N Nosik, L B Kalnina, L M Selimova, E N Kaplina","doi":"10.36233/0507-4088-240","DOIUrl":"10.36233/0507-4088-240","url":null,"abstract":"<p><strong>Introduction: </strong>The persistence of immune dysfunction during therapy has serious consequences for the health of HIV-infected people. Therefore, an important direction is the search for drugs that can reduce the inflammatory potential of the immune system and serve as an additional component of antiviral therapy. Aim ‒ to study the effect of the immunomodulatory drug Sodium deoxyribonucleate with iron complex (DNA-Na-Fe) on the expression of activation markers in MT-4 cells infected with HIV-1.</p><p><strong>Materials and methods: </strong>Expression levels of CD4, CD28, CD38, CD62L and HLA-DR proteins on the plasma membrane were measured in cells. To assess viral activity, the p24 protein was quantified by ELISA.</p><p><strong>Results and discussion: </strong>The two cell variants with different replicative activity were analyzed. Control cells, cells with DNA-Na-Fe, infected cells and infected cells with DNA-Na-Fe were tested. Based on the results obtained, it can be concluded that antiviral activity of the drug in MT-4 cells infected with HIV-1 is associated with immunomodulatory activity that enhances the expression of membrane proteins CD4, CD28, CD38 and CD62L. Diversity in the effect of DNA-Na-Fe on the studied surface proteins expression in two cell lines indicates that they depend on the characteristics of the combined molecular biological processes occurring in cells. And the increased effects observed in a system with changes in replicative activity assumes its active participation in virus replication at the stages of virus penetration and budding.</p><p><strong>Conclusion: </strong>Studies have shown that DNA-Na-Fe has antiviral and immunomodulatory activity.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"69 4","pages":"309-319"},"PeriodicalIF":0.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142373061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First detection of influenza A virus subtypes H1N1 and H3N8 in the Antarctic region: King George Island, 2023.","authors":"O V Ohlopkova, A E Goncharov, B I Aslanov, A V Fadeev, Y N Davidyuk, A D Moshkin, K A Stolbunova, M A Stepanyuk, I A Sobolev, M A Tyumentseva, A I Tyumentsev, A M Shestopalov, V G Akimkin","doi":"10.36233/0507-4088-257","DOIUrl":"10.36233/0507-4088-257","url":null,"abstract":"<p><strong>Relevance: </strong>Influenza A virus is characterized by a segmented single-stranded RNA genome. Such organization of the virus genome determines the possibility of reassortment, which can lead to the emergence of new virus variants. The main natural reservoir of most influenza A virus subtypes are wild waterfowl. Seasonal migrations gather waterfowl from all major migration routes to nesting areas near the northern and southern polar circles. This makes intercontinental spread of influenza A viruses possible. Objective ‒ to conduct molecular genetic monitoring and study the phylogenetic relationships of influenza A virus variants circulating in Antarctica in 2023.</p><p><strong>Materials and methods: </strong>We studied 84 samples of biological material obtained from birds and marine mammals in April‒May 2023 in coastal areas of Antarctica. For 3 samples, sequencing was performed on the Miseq, Illumina platform and phylogenetic analysis of the obtained nucleotide sequences of the influenza A virus genomes was performed.</p><p><strong>Results: </strong>The circulation of avian influenza virus in the Antarctic region was confirmed. Heterogeneity of the pool of circulating variants of the influenza A virus (H3N8, H1N1) was revealed. Full-length genomes of the avian influenza virus were sequenced and posted in the GISAID database (EPI_ISL_19032103, 19174530, 19174467).</p><p><strong>Conclusion: </strong>The study of the genetic diversity of influenza A viruses circulating in the polar regions of the Earth and the identification of the conditions for the emergence of new genetic variants is a relevant task for the development of measures to prevent biological threats.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"69 4","pages":"377-389"},"PeriodicalIF":0.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142373064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection of high-risk papillomaviruses and vaccination status in normal cytology in Congo.","authors":"D F Ngombe Mouabata, A L Boumba, P J Iloukou Mayakia, N R Massengo, R P Takale, D Moukassa, M M Ennaji","doi":"10.36233/0507-4088-239","DOIUrl":"10.36233/0507-4088-239","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study was to identify the molecular prevalence of high-risk HPV infection and the distribution of genotypes present in normal cytology, as well as to determine the vaccination status of our study population.</p><p><strong>Methods: </strong>110 cervical samples were taken from individuals, and 1 ml of each sample was added to the Xpert HPV cartridge in the sample compartment before it was placed in the Cepheid GeneXpert system. Detection was performed simultaneously via amplification of the <i>E6</i> and <i>E7</i> genes in five fluorescent channels (HPV16, HPV18/45, HPV31/33/35/52/58, HPV51/59, and HPV39/56/66/68a).</p><p><strong>Results: </strong>36/110 (33%) of all samples tested were positive for HPV DNA. The predominant genotypes were HPV16 (12.7%) and other pooled HR-HPV types (8.2%). All women who received the Gardasil-9 vaccine (3.6%) had HPV, and infection was associated with travel outside Africa. 96.4% of the screened individuals had not received any HPV vaccine.</p><p><strong>Conclusion: </strong>Our research confirms a widespread HR-HPV infection in our population and extends the importance of studies on the molecular prevalence of HPV, particularly in women with normal cytology and apparent good health, in view of the cruel lack of public awareness of HPV infections.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"69 4","pages":"301-308"},"PeriodicalIF":0.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142373066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune reactivity of two biological models to vaccination with inactivated vaccine QazVac against coronavirus infection COVID-19.","authors":"B S Myrzakhmetova, G A Zhapparova, K B Bisenbayeva, A S Toytanova, M S Tuyskanova, K D Zhugunissov, L B Kutumbetov","doi":"10.36233/0507-4088-222","DOIUrl":"10.36233/0507-4088-222","url":null,"abstract":"<p><strong>Introduction: </strong>Specific prevention of a number of infectious diseases has been introduced into the vaccination schedule. The production of immunoprophylactic drugs, in order to establish standard properties, including safety and specific effectiveness, requires strict adherence to manufacturing regulations, and the reliability of the results obtained requires monitoring of these parameters. The specific effectiveness of vaccine preparations is standardized according to the indicators of stimulation of specific antibody response formed in the body of vaccinated model biological objects.</p><p><strong>Objective: </strong>Determination of the immune reactivity of white mice to vaccination with the QazVac vaccine to establish the possibility of using them as a biological model in assessing the immunogenicity of the vaccine instead of Syrian hamsters.</p><p><strong>Materials and methods: </strong>The immune reactivity of model animals was assessed by the seroconversion rate, dynamics of antibody titers to the SARS-CoV-2 virus formed in the body after vaccination with the test vaccine. In the case of seropositivity of animals before administration of vaccine or placebo, the level of immune reactivity was calculated by the difference in antibody titers between control and vaccinated animals or by the difference in antibody titers before and after immunization. Specific antibodies were detected and their titer was determined using a neutralization reaction.</p><p><strong>Results: </strong>The research results showed that the tested biological models had approximately the same immune reactivity to the administration of the QazVac vaccine, confirmed by the level and dynamics of antibody titers. When analyzing the fold increase in antibody titers in comparison to those of control animals, Syrian hamsters were more reactive compared to mice. But SPF white mice were standardized in their lack of the immune reactivity to SARS-CoV-2 virus before the immunization.</p><p><strong>Conclusion: </strong>The data obtained indicate that the immune reactivity of white mice to the administration of the QazVac vaccine in terms of the rate and dynamics of the formation of virus-neutralizing antibodies is approximately equivalent to the immune reactivity of Syrian hamsters. Before immunization with the vaccine, SPF white mice, in contrast to Syrian hamsters, do not have humoral immunity specific to the SARS-CoV-2 virus. The immune reactivity equivalent to that observed of Syrian hamsters and the absence of antibodies to the SARS-CoV-2 virus at a baseline indicate the superiority of the use of white mice in assessing the immunogenicity of vaccines against COVID-19 and/or obtaining specific factors of humoral immunity.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"69 3","pages":"219-230"},"PeriodicalIF":0.0,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141601936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrolytes, Zinc and Vitamin D₃ in COVID-19 Patients with Cardiovascular Complications.","authors":"A A AlKhuzaie, E A Jabbar, B J Albadry","doi":"10.36233/0507-4088-236","DOIUrl":"10.36233/0507-4088-236","url":null,"abstract":"<p><strong>Introduction: </strong>COVID-19 is strongly linked to cardiovascular disease, with direct myocardial injury and systemic inflammation as common mechanisms. Pre-existing or infection-induced cardiovascular disease worsens the outcomes for COVID-19 patients.</p><p><strong>Materials and methods: </strong>To estimate the serum electrolytes (Na⁺, K⁺, Ca⁺⁺, Zn) and vitamin D₃, the study depended on ichroma ii device for Vitamin D₃ and Chemistry Analyzer for electrolytes in patient samples.</p><p><strong>Results: </strong>A study was conducted on 192 individuals diagnosed with COVID-19, including 35 critical cases, 53 severe cases, 54 moderate cases, and 50 individuals in a control group. The age group with the highest prevalence of infection was between 50‒69 years, while the lowest prevalence was observed in those under 30 years. The study found significant decreases in calcium, potassium, sodium, zinc, and vitamin D₃ levels among COVID-19 patients compared to the control group. Zinc and vitamin D₃ levels showed a significant correlation with sex, with males experiencing a decline in zinc levels and females having lower vitamin D₃ levels. The concentration of calcium, sodium, and zinc showed a negative correlation with age, with older patients having the lowest levels. COVID-19 patients with chronic cardiac issues and high blood pressure exhibited the lowest levels of these markers. The severity of the disease also had a detrimental impact on electrolyte levels, zinc, and vitamin D₃, with critical cases showing the lowest levels. The complications such as heart failure were associated with lower levels of potassium, sodium, and zinc.</p><p><strong>Conclusion: </strong>In conclusion, the study revealed significant associations between COVID-19 and decreased electrolyte levels, zinc, and vitamin D₃. Sex and age were found to be correlated with these markers. Patients with chronic cardiac issues and high blood pressure exhibited the lowest levels of these markers. The severity of the disease was also linked to lower electrolyte levels, zinc, and vitamin D₃. Complications such as heart failure were associated with decreased levels of potassium, sodium, and zinc.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"69 3","pages":"266-276"},"PeriodicalIF":0.0,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141601935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Enzyme immunoassay system for serological diagnosis of hemorrhagic fever with renal syndrome based on inactivated purified Puumala virus (Hantaviridae: <i>Orthohantavirus</i>)].","authors":"A P Ivanov, T K Dzagurova, S S Kurashova, R D Teodorovich, T D Klebleeva, E A Tkachenko","doi":"10.36233/0507-4088-230","DOIUrl":"10.36233/0507-4088-230","url":null,"abstract":"<p><strong>Introduction: </strong>Hemorrhagic fever with renal syndrome (HFRS) is the most common zoonotic human viral disease in the Russian Federation. More than 98% of the HFRS cases are caused by Puumala orthohantavirus (PUU). Effective serological tests are required for laboratory diagnosis of HFRS.</p><p><strong>Objective: </strong>Construction of an enzyme immunoassay (ELISA) test system for detection of specific antibodies using standard antigen in the form of highly purified inactivated PUU virus as immunosorbent.</p><p><strong>Materials and methods: </strong>Preparation of PUU virus antigen, designing the ELISA for detection of specific antibodies, developing parameters of the ELISA system, parallel titration of HFRS patients sera by fluorescent antibody technique (FAT) and the new ELISA.</p><p><strong>Results and discussion: </strong>For the first time, ELISA based on purified inactivated PUU virus as standard antigen directly absorbed onto immunoplate was developed. Parallel titration of 50 samples from HFRS patients blood sera using FAT and the developed ELISA showed high sensitivity and specificity of this ELISA, with 100% concordance of testing results and significant level of correlation between the titers of specific antibodies in the two assays.</p><p><strong>Conclusion: </strong>The ELISA based on purified inactivated PUU virus as an immunosorbent can be effectively used for HFRS serological diagnosis and for mass seroepidemiological studies.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"69 3","pages":"285-289"},"PeriodicalIF":0.0,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141601932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coronaviruses (<i>Coronaviridae</i>) of bats in the northern Caucasus and south of western Siberia.","authors":"L N Yashina, A V Zhigalin, S A Abramov, E M Luchnikova, N A Smetannikova, T A Dupal, A V Krivopalov, E D Vdovina, K A Svirin, A A Gadzhiev, B S Malyshev","doi":"10.36233//0507-4088-233","DOIUrl":"10.36233//0507-4088-233","url":null,"abstract":"<p><strong>Introduction: </strong>Bats are natural reservoirs of coronaviruses (<i>Coronaviridae</i>), which have caused three outbreaks of human disease SARS, MERS and COVID-19 or SARS-2 over the past decade. The purpose of the work is to study the diversity of coronaviruses among bats inhabiting the foothills and mountainous areas of the Republics of Dagestan, Altai and the Kemerovo region.</p><p><strong>Materials and methods: </strong>Samples of bat oral swabs and feces were tested for the presence of coronavirus RNA by reverse transcription-polymerase chain reaction (RT-PCR).</p><p><strong>Results: </strong>It has been shown that the greater horseshoe bats (<i>Rhinolophus ferrumequinum</i>), inhabiting the Republic of Dagestan, are carriers of two different coronaviruses. One of the two coronaviruses is a member of the <i>Sarbecovius</i> subgenus of the <i>Betacoronavirus </i>genus, which includes the causative agents of SARS and COVID-19. The second coronavirus is assigned to the <i>Decacovirus</i> subgenus of the <i>Alphacoronavirus</i> genus and is most similar to viruses identified among <i>Rhinolophus</i> spp. from European and Middle Eastern countries. In the Altai Republic and Kemerovo region, coronaviruses belonging to the genus <i>Alphacoronavirus</i>, subgenus <i>Pedacovirus</i>, were found in the smooth-nosed bats: Ikonnikov`s bat (<i>Myotis ikonnikovi</i>) and the eastern bat (<i>Myotis petax</i>). The virus from the Altai Republic from <i>M. ikonnikovi </i>is close to viruses from Japan and Korea, as well as viruses from <i>Myotis</i> spp. from European countries. The virus from the Kemerovo region from <i>M. petax</i> groups with coronaviruses from <i>Myotis</i> spp. from Asian countries and is significantly different from coronaviruses previously discovered in the same natural host.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"69 3","pages":"255-265"},"PeriodicalIF":0.0,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141601933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Comparative analysis of the taxonomic classification criteria for a number of groups of pathogenic DNA and RNA viruses based on genomic data].","authors":"T E Sizikova, V N Lebedev, S V Borisevich","doi":"10.36233/0507-4088-238","DOIUrl":"10.36233/0507-4088-238","url":null,"abstract":"<p><p>The basis for criteria of the taxonomic classification of DNA and RNA viruses based on data of the genomic sequencing are viewed in this review. The genomic sequences of viruses, which have genome represented by double-stranded DNA (orthopoxviruses as example), positive-sense single-stranded RNA (alphaviruses and flaviviruses as example), non-segmented negative-sense single-stranded RNA (filoviruses as example), segmented negative-sense single-stranded RNA (arenaviruses and phleboviruses as example) are analyzed. The levels of genetic variability that determine the assignment of compared viruses to taxa of various orders are established for each group of viruses.</p>","PeriodicalId":23669,"journal":{"name":"Voprosy virusologii","volume":"69 3","pages":"203-218"},"PeriodicalIF":0.0,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141601931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}