M. V. Maslova, I. Shamshin, E. Grosheva, A. S. Ilyichev
{"title":"Molecular and genetic basis of tomato resistance to major fungal diseases","authors":"M. V. Maslova, I. Shamshin, E. Grosheva, A. S. Ilyichev","doi":"10.18619/2072-9146-2023-6-28-39","DOIUrl":"https://doi.org/10.18619/2072-9146-2023-6-28-39","url":null,"abstract":"This review presents an analysis of the literature, which indicates the popularity of breeding methods in the fight against oomycete and fungal diseases of tomato: late blight (pathogen − Phytophthora infestans (Mont.) de Bary), fusarium wilt (pathogen − Fusarium oxysporum f. sp. lycopersici (Sacc.) Snyder and Hansen), early blight or alternariosis (pathogens − representatives of the genus Alternaria), cladosporiosis (pathogen−Cladosporium fulvum Cook.). Theoretical and practical achievements, the latest developments in genetics, molecular biology, plant physiology, microbiology are considered. Special attention is paid to modern information about the diversity of tomato pathogens, their morphological, genetic, physiological features, including racial composition; the presence of pathogen resistance genes in the gene pool of the culture under study, their mapping and the possibility of introduction into the genome from other sources; about the developed markers of target genes and loci of quantitative traits; the degree of associations between a molecular marker and a target gene; about the features of inheritance of the studied trait, as well as the possibility of pyramiding R-genes and QTL in one genotype. To accelerate and improve the efficiency of tomato breeding for resistance to pathogens, these issues are of great importance. Their study will strengthen the integration of modern biotechnology with the traditional breeding process, which is carried out by classical methods.","PeriodicalId":23570,"journal":{"name":"Vegetable crops of Russia","volume":"54 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138604966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The use of electrophoretic analysis to determine the polymorphism of vegetable bean varieties","authors":"E. V. Pankrutskaya, V. Skorina","doi":"10.18619/2072-9146-2023-6-40-46","DOIUrl":"https://doi.org/10.18619/2072-9146-2023-6-40-46","url":null,"abstract":"Relevance. In Belarus, no full-fledged studies have previously been carried out to identify species diversity, to identify forms of vegetable beans that have similar genetic diversity. The purpose of the research is to determine the number and ratio of biotypes, to identify the level of genetic polymorphism in vegetable bean varieties by electrophoresis of storage proteins.Materials and Methods. The research was carried out at the BSAA in 2021–2022 on soddy-podzolic medium loamy soil. The experience was based on generally accepted methods and guidelines. The objects of research were 42 varieties of vegetable beans (34 - bush form) and (8 - climbing) of Belarusian and Russian selection. Analysis of seed storage proteins in vegetable bean varieties and identification of spectra were carried out according to the methods. To assess the differentiating positions (zones) of the spectrum, identify protein components, and estimate the molecular masses of proteins, standard marker solutions of proteins “Thermo Scientific” – Unstained Protein Ladder (range 5–112 kDa, number of identified proteins – 11) were used.Results. Differences between genotypes were revealed in internal genetic diversity and elements of the protein spectrum of globulins. It has been established that vegetable bean varieties are characterized by hidden genetic variability and a certain level of polymorphism with a different number of biotypes in the structure of the varietal population. In 60% of the analyzed forms, there is a direct relationship between the manifestation of rare, selection-significant components and the degree of complexity of the total component composition of the protein spectrum. This criterion has practical significance and can be used in breeding work with the vegetable bean crop.","PeriodicalId":23570,"journal":{"name":"Vegetable crops of Russia","volume":"56 20","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138604996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fasciation in cucurbits","authors":"I. B. Korottseva","doi":"10.18619/2072-9146-2023-6-17-21","DOIUrl":"https://doi.org/10.18619/2072-9146-2023-6-17-21","url":null,"abstract":"The article presents a literature review on fasciation in pumpkin crops. In almost all pumpkin crops, you can find such a phenomenon as fasciation. However, it is more common in cucumbers and pumpkins than in melons and watermelons. Fasciation (or crystallization) is a change that can occur in the morphology of plant organs and usually includes expansion of the apical meristem of the shoot, flattening of the stem and changes in the arrangement of leaves. In pumpkin crops, the fasciated organs are often whips, flowers and fruits. A number of authors divide fasciations into inherited and non-inherited. The first are caused by internal reasons. The second is the effect of external factors, such as insect damage, injury, weather conditions. According to literature data, fasciation is caused by 1-2 recessive genes with incomplete manifestation and variable expression, which depends on a number of factors, including environmental conditions. The opp gene may have a pleiotropic effect on fasciation and leaf arrangement. In Federal Scientific Vegetable Center in spring film greenhouses, among the selection and collection samples of cucumber, fasciated plants ranged from 0 to 1.9%, depending on the genotype of the studied samples and the year of research (growing conditions). According to literature data, fasciation is more often observed in short-fruited, but it can also be detected in long-fruited forms of cucumber. Fused fruits are more likely to appear on cucumber hybrids with a large number of ovaries in the node. It should be noted, that highly fasciated plants are usually less productive, and most fasciated fruits are often classified as non-standard and rejected. Regular culling by breeders of fasciated samples and plants, within individual samples, will allow to create varieties less prone to fasciation. And compliance with the recommended varietal agrotechnics, improvement of the ecological situation will help to significantly reduce the number of fasciated plants and fruits and improve the marketability of products.","PeriodicalId":23570,"journal":{"name":"Vegetable crops of Russia","volume":"47 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138605300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. B. Korottseva, S. Belov, M. E. Sletova, N. Sakara, I. A. Vanyushkina, T. S. Tarasova, N. A. Sinichenko
{"title":"Evaluation of promising cucumber lines of the parthenocarpic type for resistance to false powdery mildew in open ground conditions of the Moscow region","authors":"I. B. Korottseva, S. Belov, M. E. Sletova, N. Sakara, I. A. Vanyushkina, T. S. Tarasova, N. A. Sinichenko","doi":"10.18619/2072-9146-2023-6-5-10","DOIUrl":"https://doi.org/10.18619/2072-9146-2023-6-5-10","url":null,"abstract":"","PeriodicalId":23570,"journal":{"name":"Vegetable crops of Russia","volume":"48 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138605359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correlations between some quantitative traits of cucumber F1 parthenocarpic hybrids with a smooth fruit type grown in plastic greenhouses","authors":"N. Khomchenko, V. N. Shevkunov","doi":"10.18619/2072-9146-2023-6-22-27","DOIUrl":"https://doi.org/10.18619/2072-9146-2023-6-22-27","url":null,"abstract":"","PeriodicalId":23570,"journal":{"name":"Vegetable crops of Russia","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138605259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Н. Зеленков, Анатолий Александрович Кособрюхов, Борис Михайлович Новиков, Людмила Николаевна Путилина, М. И. Иванова, П. А. Верник, С. В. Гаврилов, V. V. Latushkin, V. N. Zelenkov, A. Kosobryukhov, V. Novikov, L. Putilina, M. Ivanova, P. Vernik, S. V. Gavrilov
{"title":"Influence of photons of the near-ultraviolet radiation on the growth and development of sugar beet (Beta vulgaris L. ssp. vulgaris var. saccharifera Alef.) in a closed agrobiotechnosystem","authors":"Н. Зеленков, Анатолий Александрович Кособрюхов, Борис Михайлович Новиков, Людмила Николаевна Путилина, М. И. Иванова, П. А. Верник, С. В. Гаврилов, V. V. Latushkin, V. N. Zelenkov, A. Kosobryukhov, V. Novikov, L. Putilina, M. Ivanova, P. Vernik, S. V. Gavrilov","doi":"10.18619/2072-9146-2023-4-49-57","DOIUrl":"https://doi.org/10.18619/2072-9146-2023-4-49-57","url":null,"abstract":"Relevance and methodology. In order to determine the effect of near-ultraviolet radiation with a wavelength of 380 nm on the growth and development of a sugar beet hybrid plant, Smena was grown for 82 days under LED lighting with phytolamps and under conditions of increased UV-A intensity of the light range (an increase in the UV/PPFD ratio (0.027) compared with the control (0.0075) while maintaining the ratio of the remaining sites spectrum). The study was carried out on the basis of the digital software package \"Synergotron\" with a controlled internal environment.Results. An increase in the share of UV-A in the illumination spectrum leads to a significant change in the biometric indicators of plants – the aboveground biomass increases by 2.2 times compared to the control, and the mass of the underground part (root crops), on the contrary, decreases by 86.9%. At the same time, the share of root crops in the total biomass of plants decreases from 60% in the control to 30%. The morphological structure of the leaf apparatus changes: the proportion of petioles increases significantly compared to leaf blades (64.8% of petioles in aboveground biomass, whereas in the control 30%). Probably, an increase in the share of UV-A in the spectrum can favorably affect the cultivation of leaf forms of beets and other root crops. UV-A radiation leads to a change in the chemical composition of root crops, in particular, a decrease in the accumulation of dry substances (by 1.58%) and a decrease in sugar content (by 1.8%). An increase in the proportion of UV-A in the irradiation spectrum changes the parameters of chlorophyll fluorescence and contributes to an increase in the maximum quantum yield of Fv/Fm, non-photosynthetic quenching of NPQ fluorescence and a decrease in the real quantum yield of photosynthesis Y(II), as well as the electron transport rate (ETR).","PeriodicalId":23570,"journal":{"name":"Vegetable crops of Russia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77230462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comprehensive assessment of the parameters of adaptive capacity and ecological stability of genotypes for winter garlic breeding","authors":"V. Skorina, Vit. V. Skoryna","doi":"10.18619/2072-9146-2023-4-58-61","DOIUrl":"https://doi.org/10.18619/2072-9146-2023-4-58-61","url":null,"abstract":"Relevance. Considering the regional peculiarities of the climate of Belarus in the cultivation of garlic, it is relevant to create varieties with high yields, environmental stability, winter hardiness and product quality in changing environmental conditions. The aim of the research is a comprehensive assessment of the genotypes of winter garlic according to the parameters of adaptability and the selection of the most stable samples among them.Materials and methods. The research was carried out on the experimental field of the Department of Fruit and Vegetable Growing of the Belarusian State Agricultural Academy, Gorki, Mogilev region during 2018-2022. The objects were collectible samples of winter garlic of various ecological and geographical origin. Field and laboratory experiments were carried out using generally accepted methods and guidelines. Meteorological conditions during the years of research differed both in temperature indicators and the amount of precipitation, which contributed to an objective assessment of the studied samples according to a complex of economically useful signs and the determination of parameters of adaptive capacity and environmental stability.Results. As a result a comprehensive assessment of the parameters of adaptive capacity and environmental stability of genotypes was given. The results of the data obtained when studying the reaction of genotypes to the medium showed that 44% (11) of the 25 genotypes were unstable with a positive reaction to the medium, and 56% (14) were stable. Studies have revealed diversity among genotypes in terms of the main parameters of adaptability, including SCGi. The analysis of the data obtained makes it possible to determine the common properties of genotypes with a high level of the SCGi parameter or the differences between them and other issues of the specificity of genotypes with a different combination of adaptability and stability parameters. The specificity consists in a combination of maximum values of the level of parameters Xi, OASi, CACi, with significant responsiveness to improving environmental conditions (bi) and average relative stability. A genotype with a low value of the SCGi parameter can be highly stable and serve as a source of this property. In the opposite case, it can be used as a parent form to transmit to the offspring the properties of productivity in combination with stability.","PeriodicalId":23570,"journal":{"name":"Vegetable crops of Russia","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73069634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Э. Лазько, Э. У. Тайшибаева, E. Varivoda, V. Lazko, Elvira U. Taishibaeva
{"title":"Ecological testing of melon varieties bred at the Bykovskaya Experimental Station","authors":"Э. Лазько, Э. У. Тайшибаева, E. Varivoda, V. Lazko, Elvira U. Taishibaeva","doi":"10.18619/2072-9146-2023-4-82-86","DOIUrl":"https://doi.org/10.18619/2072-9146-2023-4-82-86","url":null,"abstract":"Relevance and methodology. Currently, due to changing weather conditions, the cultivation of melons and gourds is shifting to areas that were not previously engaged in melon growing. Therefore, the testing of available varieties in various environmental conditions is relevant. The purpose of this work is to evaluate melon varieties bred at the Bykovskaya melon breeding experimental station in various soil and climatic conditions. Three varieties of melon were used as the object of research. The tests were carried out in the conditions of the Volgograd region, the Krasnodar region and the Republic of Kazakhstan. During the research, the melon varieties were evaluated according to the following indicators: yield, duration of the growing season, dry matter content.Results. As a result of the research, it was revealed that the variety Harmonia was the most stable in terms of the duration of the growing season (75-79 days). A consistently high content of dry matter in all areas of the study was in the varieties Comet and Idyll from 12.0 to 15.0%. All varieties had a good tasting score. The average fruit weight of all melon varieties grown in Krasnodar and the Volgograd region was at the same level. The high average weight in the conditions of Kazakhstan was distinguished by the Idyll variety - 4.3 kg. The melon varieties Harmoniya and Idyllia have a consistently high yield in all three zones. The variety Kometa had the highest yield in the conditions of Kazakhstan - 16.3 t/ha. Thus, melon varieties that have been tested in different regions of cultivation have high quality indicators and yields and are suitable for growing marketable products in the regions of the study.","PeriodicalId":23570,"journal":{"name":"Vegetable crops of Russia","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90871949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Zhemchuzhina, S. A. Elizarova, M. I. Kiseleva, D. A. Zakharov, I. Sardarova
{"title":"Experimental substantiation of the method for assessing the phytotoxicity of micromycetes on wheat seedlings","authors":"N. Zhemchuzhina, S. A. Elizarova, M. I. Kiseleva, D. A. Zakharov, I. Sardarova","doi":"10.18619/2072-9146-2023-4-97-106","DOIUrl":"https://doi.org/10.18619/2072-9146-2023-4-97-106","url":null,"abstract":"Relevance. Currently, the relevance of studying to identify the ability of many micromycetes to synthesize phytotoxic substances that reduce seed germination and lead to huge losses in the yield of strategic agricultural crops is increasing. To solve the problem, the development of new methodological approaches is required, which is significant for immunologists and phytopathologists.The purpose of this work is to determine the phytotoxic activity of micromycete strains from the genera Fusarium, Alternaria, Bipolaris, parasitizing on all agricultural crops cultivated in Russia.Materials and methods. The strains, the methods of their cultivation, the bioassay method were used, and the statistical processing of the results obtained was carried out. A modification of some methodological approaches for assessing the phytotoxicity of micromycetes has been carried out. The selection of representative concentrations of culture fluid filtrates (FCF) of strains for phytotoxicity evaluation was carried out.Results. A correlation was established for all variants of the experiment between two independent parameters: the development parameters of wheat seedlings and the concentration of cultural liquid filtrates. New results have been obtained on the study of the phytotoxic activity of 70 strains of hemibiotrophic micromycetes and the optimal concentrations of fungal FAs for classifying fungal strains into toxicity groups have been established. The most variable toxicity values were found when using a 40% solution of FCL. It was shown that, under these conditions, fungal strains can be divided according to the degree of toxicity into significantly different groups. This circumstance is an important reason for including strains of micromycetes with certain toxicity properties in the State Collection of Phytopathogenic Microorganisms. Fungal strains are necessary and appropriate for use in breeding to create resistant and tolerant varieties to phytopathogens from the genera Fusarium, Alternaria, Bipolaris.Conclusion. New methodological approaches have confirmed that the most variable toxicity values were found with the use of FCF in a ratio of 2:3. This concentration of FCF strains are divided into 4 groups with characteristic differences in the degree of toxicity, which is an important reason for including such strains of micromycetes in the State Collection of Phytopathogenic Microorganisms and will be further used in breeding to create resistant and tolerant varieties of agricultural crops.","PeriodicalId":23570,"journal":{"name":"Vegetable crops of Russia","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85932865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Use of water-soluble microfertilizers for growing meteor table variety watermelon","authors":"E. Galichkina, S. M. Nadezhdkin, D. A. Makarenkov","doi":"10.18619/2072-9146-2023-4-87-91","DOIUrl":"https://doi.org/10.18619/2072-9146-2023-4-87-91","url":null,"abstract":"Relevance. In connection with the introduction of various types of chelated microfertilizers into production, it is necessary to develop new elements of varietal technology for the cultivation of melons and gourds in order to obtain stable yields in the arid conditions of the Volgograd Trans-Volga region. These studies will be relevant in this period of time.Material and technique. The object of research is watermelon, variety Meteor. We studied the types and methods of using chelate microfertilizers: Helaton Extra, Chelate B, Chelate Fe. Fertilizers were used for soaking seeds before sowing and for foliar treatment of plants during the growing season.Results. During the research period of 2019-2021, new forms of microfertilizers were studied. As a result of the research, new preparations for different methods of processing had a positive effect on the growth and development of table watermelon plants. The evaluation of the results obtained showed a positive effect of new microfertilizers on the germination energy and seed germination. The maximum growth of lashes was recorded when seeds were soaked before sowing: Helaton Extra preparation and foliar feeding of plants during the growing season with microfertilizer boron chelate. The best results of increasing the leaf surface were obtained in variants with the use of Helaton Extra microfertilizer for soaking seeds and treating plants on vegetative organs. Accordingly, the highest yield was obtained when using Helaton Extra fertilizer for seed treatment before sowing and spraying plants during the growing season. The output of marketable products was noted at a fairly high level. The average weight of the fetus in all studied variants ranged from 5.9 kg to 7.8 kg. The length of the growing season in all variants increased slightly. Comparative analysis of growth processes and productivity of watermelon variety Meteor when using new forms of water-soluble fertilizers for seed soaking and foliar treatment of plants showed greater efficiency from their use.","PeriodicalId":23570,"journal":{"name":"Vegetable crops of Russia","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75494402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}