Yakup Yildirim, Ali Küçük, Mehmet Kale, Sibel Hasircioğlu, Kamil Atli, Hasbi Sait Saltik
{"title":"Evaluating the Efficiency of Various Treatment Methods in Cattle Cutaneous Papillomatosis.","authors":"Yakup Yildirim, Ali Küçük, Mehmet Kale, Sibel Hasircioğlu, Kamil Atli, Hasbi Sait Saltik","doi":"10.12834/VetIt.3138.21450.2","DOIUrl":"https://doi.org/10.12834/VetIt.3138.21450.2","url":null,"abstract":"<p><p>In this study, we compared the effectiveness of various methods used in the treatment of cattle with cutaneous papillomatosis. Ivermectin, Tarantula cubensis extract, levamisole, autovaccine, and a combination of T. cubensis extract + levamisole were administered to the animals. The animals were divided into six equal groups. Animals in the control group (n = 10) did not receive any treatment. The animals in the experimental group were administered Ivermectin [three times a week, n = 10, subcutaneous, (SC)], Tarantula cubensis extract (twice a week, n = 10, SC), autologous vaccine (three times at 10-day intervals, n = 10, SC), levamisole [twice at one-week intervals, n = 10, intramuscular (IM)], and levamisole + Tarantula cubensis extract (concurrently). All animals used in the study were monitored for three months at an interval of 15 days. No regression was detected in the papillomas of the control group animals, but recovery was recorded in animals treated with ivermectin at a rate of 70% (7/10), while it was 60% (6/10) in those treated with T. cubensis extract, 100% (10/10) in those treated with autovaccine, 50% (5/10) in those treated with levamisole, and 90% (9/10) in those treated with the combination of T. cubensis extract + levamisole. Significant differences were found between the control group and all treatment groups. Recovery mostly occurred within 45-60 days (P < 0.05). The five treatment modalities applied for the treatment of bovine cutaneous papillomatosis were statistically evaluated and all methods of treatment were effective at different rates. The most precise and effective treatment method was the autovaccine one.</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"59 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140959908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sapna Prajapati, Gururaj Kumaresan, Manish Kumar, Dimple Andani, Anil Kumar Mishra, Ashok Kumar, R V S Pawaiya, Nitika Sharma, Anu Rahal, Dinesh Kumar Sharma
{"title":"Occurrence of enteric viruses causing clinical diarrhea in small ruminants in northern Indian plains: a reverse transcription PCR based molecular study.","authors":"Sapna Prajapati, Gururaj Kumaresan, Manish Kumar, Dimple Andani, Anil Kumar Mishra, Ashok Kumar, R V S Pawaiya, Nitika Sharma, Anu Rahal, Dinesh Kumar Sharma","doi":"10.12834/VetIt.3005.20299.2","DOIUrl":"https://doi.org/10.12834/VetIt.3005.20299.2","url":null,"abstract":"<p><p>Fowlpox virus (FPV) infects chickens and turkeys giving rise to pock lesions on various body parts like combs, wattles, legs, shanks, eyes, mouth, etc. The birds, affected with FPV, also show anemia and a ruffled appearance which are clinical symptoms of reticuloendotheliosis. Interestingly, the field strains of FPV are integrated with the provirus of reticuloendotheliosis virus (REV). Due to this integration, the infected birds, upon replication of FPV, give rise to free REV virions, causing severe immunosuppression and anemia. Pox scabs, collected from the infected birds, not only show positive PCR results upon performing FPV-specific 4b core protein gene PCR but also show positive results for the PCR of REV-specific env gene and FPV-REV 5'LTR junction. Homogenized suspension of the pock lesions, upon inoculating to the chorio-allantoic membrane (CAM) of 10-day-old specific pathogen-free embryonated chicken eggs, produces characteristic pock lesions in serial passages. However, the lesions also harbor REV mRNA or free virion, which can be identified by performing REV-specific env gene PCR using REV RNA from FPV-infected CAMs. The study suggests successful replication and availability of REV mRNA and free virion alongside the FPV, although the CAM is an ill-suited medium for any retroviral (like REV) growth and replication.</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"59 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140959922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Richard Adeleke, Tobi Olanipekun, John Abiola, Adegboyega Aluko, Waidi Sule, Daniel Oluwayelu
{"title":"Serological Investigation of Japanese Encephalitis Virus Infection in Commercially Reared Pigs, Southwestern Nigeria.","authors":"Richard Adeleke, Tobi Olanipekun, John Abiola, Adegboyega Aluko, Waidi Sule, Daniel Oluwayelu","doi":"10.12834/VetIt.3021.20221.2","DOIUrl":"https://doi.org/10.12834/VetIt.3021.20221.2","url":null,"abstract":"<p><p>Japanese encephalitis virus (JEV) is a zoonotic arbovirus that causes abortion, stillbirth, and congenital defects in pigs, and epidemic encephalitis in humans. Currently, there is scarcity of information on JEV infection in pigs in Nigeria. Since the Culex tritaeniorhynchus vector of JEV is present in Nigeria and considering recent anecdotal reports of abortions and birth of weak piglets in some pig farms in southwestern Nigeria, there is a need for studies on the presence of the virus and its true burden among pig populations in the country. Serum samples (n=368) obtained from farm-reared pigs in four States of southwestern Nigeria were screened for JEV-specific IgG antibodies using a commercial ELISA kit. An overall JEV seropositivity of 35.1% (95% CI: 30.18 - 39.93%) was obtained, with detectable antibodies in pigs of all age groups, breeds, sex, and locations. Our results suggest natural exposure of these unvaccinated intensively reared pigs to JEV circulating silently in the swine population with significant association of the seropositivity with location (state/community in which the pig farms exist) and breed of the pigs studied. This first report of detection of anti-JEV antibodies in pigs in Nigeria indicates that JEV circulated among these pigs and underscores the need for active surveillance for JEV in humans, pigs, and mosquitoes to provide valuable epidemiological data for the design of effective control strategies against the virus, thus forestalling potential future outbreaks of the infection.</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"59 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140959925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Canine Distemper Virus (CDV) in Grey Wolf (Canis lupus) in Fars Province of Iran.","authors":"Ehsan Saeidi, Foozhan Kheradmand","doi":"10.12834/VetIt.3067.20864.2","DOIUrl":"10.12834/VetIt.3067.20864.2","url":null,"abstract":"<p><p>Emerging and re-emerging viral diseases shared between wildlife and domestic animals are continually spreading to new geographic locations, influenced by human activities and environmental change. Canine distemper (CD) is probably one of the best examples of a disease that has been proved to be capable of compromising the conservation of several wild carnivore species. In this article, we describe a case report of CD in a grey wolf (Canis lupus) in Iran. A grey wolf was found in Fars Province close to Bamou national park. Clinical signs were characterized by neurologic signs, muscle twitching, hyperkeratosis of the footpads and nose and keratoconjunctivitis sicca. After the death of the animal, samples were taken from different organs and sent to collaborator laboratory of Fars Provincial Office of Veterinary Organization. RT-PCR assays confirmed canine distemper virus in the grey wolf. This is the first documented report of canine distemper virus in wild species from Fars Province of Iran.</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"59 4","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141200466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Josiane Ito Eleodoro, Luiza Muraga, Rogério Luiz Vanot, Rafael Fagnani
{"title":"Identification and antimicrobial susceptibility of milk isolates from cows with subclinical mastitis in the northwest of Paraná State, Brazil.","authors":"Josiane Ito Eleodoro, Luiza Muraga, Rogério Luiz Vanot, Rafael Fagnani","doi":"10.12834/VetIt.2606.16215.1","DOIUrl":"https://doi.org/10.12834/VetIt.2606.16215.1","url":null,"abstract":"<p><p>Bovine mastitis is a multifactorial disease, caused mainly by bacteria, whose most traditional method of treatment is the use of antibiotics. However, incorrect administration of these drugs may lead to the emergence of resistant strains. The objective of this study was to identify the most common pathogens causing subclinical mastitis in the northwest State of Paraná, Brazil and establish their antimicrobial susceptibility profiles. A total of 507 foremilk samples were collected in 13 municipalities. Bacteria were identified and their profile of antimicrobial susceptibility were tested. The distribution of positive samples was not homogeneous among the cities. Streptococci were the most frequently genus observed (61%), followed by staphylococci (28%) and other genera (11%). Among streptococci and staphylococci, Streptococcus uberis and Staphylococcus aureus were the most frequently identified species, respectively. Overall, 40% of the isolates showed resistance to at least one of the antibiotics used in this study. A high proportion of cefoperazone resistant Staphylococcus spp. and bacitracin resistant Streptococcus were observed. The isolated microorganisms showed little resistance to enrofloxaxin. The results obtained show the importance of isolating the microorganism causing mastitis and testing its resistance to antibiotics before performing the treatment of the disease.</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138462907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Survey on husbandry and cheese manufacturing practices in small ruminants' farmhouse dairies in Central Italy.","authors":"Ziad Mezher, Evgeniya Titarenko, Valeria Morena, Gilberto Giangolini, Roberto Condoleo","doi":"10.12834/VetIt.2687.19358.2","DOIUrl":"https://doi.org/10.12834/VetIt.2687.19358.2","url":null,"abstract":"<p><p>Farmhouse cheeses made from raw ovine or caprine milk are very popular among the consumers not only in Italy but also overseas because of their unique organoleptic properties. These cheeses are usually manufactured, according to traditional methods, in small rudimental facilities adjacent to the farm where the achievement of satisfactory hygienic standards can be challenging. However, the lack of systematic data about farm management and the cheese manufacturing processes hampers the conduction of specific risk assessment studies. In order to fill the knowledge gaps, we collected relevant data, through a questionnaire - based survey, from 125 small ruminants' farmhouse dairies spread in Lazio. Results showed that 1.1% of registered farms process their own milk for the production of raw milk cheeses. Hand milking is still applied in almost half of them and most products are subject to a short ‑ to ‑ medium ripening period which might not be sufficient to reduce eventual pathogen load. Products are mainly sold directly to consumers on the farm premises. Our results suggest the need to support these artisan cheese producers in order to improve the production standards without altering the traditional cheesemaking practices. The reported data are also useful for specific risk assessment studies.</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"59 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140946168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pneumorrhachis in a cat: case study and a literature review.","authors":"Roman Aleksiewicz, Mikołaj Paściak","doi":"10.12834/VetIt.2961.19671.2","DOIUrl":"10.12834/VetIt.2961.19671.2","url":null,"abstract":"<p><p>A three‑year‑old domestic shorthair cat was referred for treatment of pelvic limb paralysis. Imaging examinations revealed air in the spinal canal (pneumorrhachis). A right‑sided hemilaminectomy was performed to decompress the spinal cord, which led to full resolution of the symptoms within four weeks after the procedure. To our knowledge, this is the first reported case of spontaneous pneumorrhachis in a cat. As there are no treatment standards, we would like to add to their creation. The available literature on pneumorrhachis has also been reviewed.</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"59 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140946167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bovine papillomavirus in Egypt: clinico-pathological features and molecular evolutionary analysis.","authors":"Sherin Rouby, Samar Ewies","doi":"10.12834/VetIt.3088.21173.2","DOIUrl":"https://doi.org/10.12834/VetIt.3088.21173.2","url":null,"abstract":"<p><p>Bovine papillomatosis is an infectious viral disease of cattle characterized by development of benign cutaneous warts. The present study describes bovine papillomavirus infection in cattle on clinco-pathological and molecular bases and compares the identified strains with the previously characterized papillomavirus isolates in Egypt either of bovine or equine origin. Out of sixty examined cattle, skin lesions were collected from eleven clinically diseased cattle exhibiting typical papillomatosis clinical signs and subjected to histopathological and molecular identification. Histological sections showed well-developed papillary projections of squamous epithelium associated with fibrovascular stroma. Type 1 bovine papillomavirus (BPV-1) was identified in the cutaneous lesions based on the results of L1 gene-based PCR using degenerated primer followed by DNA sequencing. Comparative sequence and evolutionary analysis revealed that papilloma sequences (OP777901, OP777902, OP777903) obtained in the current study are clustered along with MW018705.1, MG547343.1isolated from cattle in Egypt in 2017/2018 and MT502095.1.1, and MT502105.1 isolated from equine in Egypt in 2019. Results prove the circulation of BPV-1 in the areas under investigation and shed light on the role of multispecies grazing in Egypt as a risk factor for transmission of BPV-1 from cattle to horses.</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"59 3","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Federica Cagnasso, Luisa Rambozzi, Barbara Miniscalco, Anna Rita Molinar Min, Franca Borella, Antonio Borrelli, Paola Gianella
{"title":"Strongyloides stercoralis infection and long-term follow-up in a privately-owned dog from north-west of Italy.","authors":"Federica Cagnasso, Luisa Rambozzi, Barbara Miniscalco, Anna Rita Molinar Min, Franca Borella, Antonio Borrelli, Paola Gianella","doi":"10.12834/VetIt.2969.19642.2","DOIUrl":"10.12834/VetIt.2969.19642.2","url":null,"abstract":"<p><p>Strongyloides stercoralis is a zoonotic parasite, infecting human, cats and dogs. The infection is typical of tropical and subtropical areas, while it is underestimated in temperate countries. In Europe, data from humans demonstrate a large variability of infection prevalence; while scarce literature is available for dogs, with most cases reported in kennel dogs from southern Italy. A privately owned 8-month-old French Bulldog was examined because of chronic cough, diarrhea, and poor body conditions. Radiography of the thorax revealed a diffuse bronchial pattern and abdominal ultrasound showed unspecific signs of inflammation. Cytological analysis of a rectal swab revealed the presence of larvae compatible with Strongyloides sp. A parasitic infection causing protein-losing enteropathy and bronchopneumonia was suspected and fenbendazole was administered with a poor clinical response. Fecal Baermann test revealed S. stercoralis rhabditiform larvae and thinshelled eggs. Clinical signs resolved progressively after two subcutaneous ivermectin administrations (off-label) given two weeks apart. Serial coprological controls scheduled in the following 6 months were negative for larvae and eggs. Fecal real-time PCR confirmed negative results 6 months after treatment. The authors describe for the first time a case of S. stercoralis infection in a privately-owned dog from north-west of Italy (Piedmont).</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"59 3","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141580954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Gastrointestinal and respiratory parasites in captive mammals at Rabat zoo, with the first record of Capillaria spp. in the Fennec fox (Vulpes zerda).","authors":"Yahya Taki, Maria Bourquia","doi":"10.12834/VetIt.2856.19877.2","DOIUrl":"https://doi.org/10.12834/VetIt.2856.19877.2","url":null,"abstract":"<p><p>The aim of the present study was to broaden the understanding of parasitism in captive wild mammals in Morocco. For this purpose, an investigation on gastrointestinal and respiratory parasites in African mammals from Rabat Zoo was carried out. A total of 47 fecal samples were collected from 30 species from November 2021 to March 2022 and examined macroscopically and microscopically. Parasites were detected in 21 species at a prevalence of 70%, with a parasite positivity rate of 89% in artiodactyls, 50% in perissodactyls, 67% in both carnivores and primates. No parasitic infection was detected in proboscideans. The most frequent infection was by nematodes with a percentage of 50%, followed by both protozoa and mixed infection with a prevalence of 10%. In conclusion, the results demonstrated that parasite prevalence remains high, even in enclosed spaces like zoos. Therefore, in order to ensure animal welfare and staff safety, sanitary measures should be implemented in such facilities, including routine diagnostic tests followed by appropriate treatment.</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":"59 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140851432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}