Josiane Ito Eleodoro, Luiza Muraga, Rogério Luiz Vanot, Rafael Fagnani
{"title":"Identification and antimicrobial susceptibility of milk isolates from cows with subclinical mastitis in the northwest of Paraná State, Brazil.","authors":"Josiane Ito Eleodoro, Luiza Muraga, Rogério Luiz Vanot, Rafael Fagnani","doi":"10.12834/VetIt.2606.16215.1","DOIUrl":"https://doi.org/10.12834/VetIt.2606.16215.1","url":null,"abstract":"<p><p>Bovine mastitis is a multifactorial disease, caused mainly by bacteria, whose most traditional method of treatment is the use of antibiotics. However, incorrect administration of these drugs may lead to the emergence of resistant strains. The objective of this study was to identify the most common pathogens causing subclinical mastitis in the northwest State of Paraná, Brazil and establish their antimicrobial susceptibility profiles. A total of 507 foremilk samples were collected in 13 municipalities. Bacteria were identified and their profile of antimicrobial susceptibility were tested. The distribution of positive samples was not homogeneous among the cities. Streptococci were the most frequently genus observed (61%), followed by staphylococci (28%) and other genera (11%). Among streptococci and staphylococci, Streptococcus uberis and Staphylococcus aureus were the most frequently identified species, respectively. Overall, 40% of the isolates showed resistance to at least one of the antibiotics used in this study. A high proportion of cefoperazone resistant Staphylococcus spp. and bacitracin resistant Streptococcus were observed. The isolated microorganisms showed little resistance to enrofloxaxin. The results obtained show the importance of isolating the microorganism causing mastitis and testing its resistance to antibiotics before performing the treatment of the disease.</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138462907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Survey on husbandry and cheese manufacturing practices in small ruminants' farmhouse dairies in Central Italy.","authors":"Ziad Mezher, Evgeniya Titarenko, Valeria Morena, Gilberto Giangolini, Roberto Condoleo","doi":"10.12834/VetIt.2687.19358.2","DOIUrl":"https://doi.org/10.12834/VetIt.2687.19358.2","url":null,"abstract":"<p><p>Farmhouse cheeses made from raw ovine or caprine milk are very popular among the consumers not only in Italy but also overseas because of their unique organoleptic properties. These cheeses are usually manufactured, according to traditional methods, in small rudimental facilities adjacent to the farm where the achievement of satisfactory hygienic standards can be challenging. However, the lack of systematic data about farm management and the cheese manufacturing processes hampers the conduction of specific risk assessment studies. In order to fill the knowledge gaps, we collected relevant data, through a questionnaire - based survey, from 125 small ruminants' farmhouse dairies spread in Lazio. Results showed that 1.1% of registered farms process their own milk for the production of raw milk cheeses. Hand milking is still applied in almost half of them and most products are subject to a short ‑ to ‑ medium ripening period which might not be sufficient to reduce eventual pathogen load. Products are mainly sold directly to consumers on the farm premises. Our results suggest the need to support these artisan cheese producers in order to improve the production standards without altering the traditional cheesemaking practices. The reported data are also useful for specific risk assessment studies.</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140946168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pneumorrhachis in a cat: case study and a literature review.","authors":"Roman Aleksiewicz, Mikołaj Paściak","doi":"10.12834/VetIt.2961.19671.2","DOIUrl":"10.12834/VetIt.2961.19671.2","url":null,"abstract":"<p><p>A three‑year‑old domestic shorthair cat was referred for treatment of pelvic limb paralysis. Imaging examinations revealed air in the spinal canal (pneumorrhachis). A right‑sided hemilaminectomy was performed to decompress the spinal cord, which led to full resolution of the symptoms within four weeks after the procedure. To our knowledge, this is the first reported case of spontaneous pneumorrhachis in a cat. As there are no treatment standards, we would like to add to their creation. The available literature on pneumorrhachis has also been reviewed.</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140946167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Federica Cagnasso, Luisa Rambozzi, Barbara Miniscalco, Anna Rita Molinar Min, Franca Borella, Antonio Borrelli, Paola Gianella
{"title":"Strongyloides stercoralis infection and long-term follow-up in a privately-owned dog from north-west of Italy.","authors":"Federica Cagnasso, Luisa Rambozzi, Barbara Miniscalco, Anna Rita Molinar Min, Franca Borella, Antonio Borrelli, Paola Gianella","doi":"10.12834/VetIt.2969.19642.2","DOIUrl":"10.12834/VetIt.2969.19642.2","url":null,"abstract":"<p><p>Strongyloides stercoralis is a zoonotic parasite, infecting human, cats and dogs. The infection is typical of tropical and subtropical areas, while it is underestimated in temperate countries. In Europe, data from humans demonstrate a large variability of infection prevalence; while scarce literature is available for dogs, with most cases reported in kennel dogs from southern Italy. A privately owned 8-month-old French Bulldog was examined because of chronic cough, diarrhea, and poor body conditions. Radiography of the thorax revealed a diffuse bronchial pattern and abdominal ultrasound showed unspecific signs of inflammation. Cytological analysis of a rectal swab revealed the presence of larvae compatible with Strongyloides sp. A parasitic infection causing protein-losing enteropathy and bronchopneumonia was suspected and fenbendazole was administered with a poor clinical response. Fecal Baermann test revealed S. stercoralis rhabditiform larvae and thinshelled eggs. Clinical signs resolved progressively after two subcutaneous ivermectin administrations (off-label) given two weeks apart. Serial coprological controls scheduled in the following 6 months were negative for larvae and eggs. Fecal real-time PCR confirmed negative results 6 months after treatment. The authors describe for the first time a case of S. stercoralis infection in a privately-owned dog from north-west of Italy (Piedmont).</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141580954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bovine papillomavirus in Egypt: clinico-pathological features and molecular evolutionary analysis.","authors":"Sherin Rouby, Samar Ewies","doi":"10.12834/VetIt.3088.21173.2","DOIUrl":"https://doi.org/10.12834/VetIt.3088.21173.2","url":null,"abstract":"<p><p>Bovine papillomatosis is an infectious viral disease of cattle characterized by development of benign cutaneous warts. The present study describes bovine papillomavirus infection in cattle on clinco-pathological and molecular bases and compares the identified strains with the previously characterized papillomavirus isolates in Egypt either of bovine or equine origin. Out of sixty examined cattle, skin lesions were collected from eleven clinically diseased cattle exhibiting typical papillomatosis clinical signs and subjected to histopathological and molecular identification. Histological sections showed well-developed papillary projections of squamous epithelium associated with fibrovascular stroma. Type 1 bovine papillomavirus (BPV-1) was identified in the cutaneous lesions based on the results of L1 gene-based PCR using degenerated primer followed by DNA sequencing. Comparative sequence and evolutionary analysis revealed that papilloma sequences (OP777901, OP777902, OP777903) obtained in the current study are clustered along with MW018705.1, MG547343.1isolated from cattle in Egypt in 2017/2018 and MT502095.1.1, and MT502105.1 isolated from equine in Egypt in 2019. Results prove the circulation of BPV-1 in the areas under investigation and shed light on the role of multispecies grazing in Egypt as a risk factor for transmission of BPV-1 from cattle to horses.</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"First report of the hard tick Ixodes Ricinus on dogs in Lebanon.","authors":"Walid Darwiche","doi":"10.12834/VetIt.2743.18431.2","DOIUrl":"https://doi.org/10.12834/VetIt.2743.18431.2","url":null,"abstract":"<p><p>Ixodes ricinus is a hard tick that can infest a large array of hosts. It is mostly abundant in the cool, humid deciduous and coniferous woods and forests of Europe. All I. ricinus life stages are potential vectors of many diseases, most notably Lyme disease, which affects millions of people yearly. Lebanon, an Asian Mediterranean country with mild cool winters and hot dry summers, lacks many reports concerning ticks. A study on tick abundance and identification on dogs presented to veterinary facilities, over the course of the years 2020 and 2021 was done. Several species of ticks were identified, with the least abundant being I. ricinus. To the best of our knowledge, this is the first report of the tick species in the country. More studies on the distribution and abundance of I. ricinus in Lebanon should be made in order to properly estimate the pathogens it carries, and consequently implement the appropriate prophylactic measures concerning public health.</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141427749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Gastrointestinal and respiratory parasites in captive mammals at Rabat zoo, with the first record of Capillaria spp. in the Fennec fox (Vulpes zerda).","authors":"Yahya Taki, Maria Bourquia","doi":"10.12834/VetIt.2856.19877.2","DOIUrl":"https://doi.org/10.12834/VetIt.2856.19877.2","url":null,"abstract":"<p><p>The aim of the present study was to broaden the understanding of parasitism in captive wild mammals in Morocco. For this purpose, an investigation on gastrointestinal and respiratory parasites in African mammals from Rabat Zoo was carried out. A total of 47 fecal samples were collected from 30 species from November 2021 to March 2022 and examined macroscopically and microscopically. Parasites were detected in 21 species at a prevalence of 70%, with a parasite positivity rate of 89% in artiodactyls, 50% in perissodactyls, 67% in both carnivores and primates. No parasitic infection was detected in proboscideans. The most frequent infection was by nematodes with a percentage of 50%, followed by both protozoa and mixed infection with a prevalence of 10%. In conclusion, the results demonstrated that parasite prevalence remains high, even in enclosed spaces like zoos. Therefore, in order to ensure animal welfare and staff safety, sanitary measures should be implemented in such facilities, including routine diagnostic tests followed by appropriate treatment.</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140851432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aleena Kokab, Ali Shekh, Masood Rabbani, Wasim Shehzad, Muhammad Ilyas Riaz, Qurat Ul Ain
{"title":"Post-harvest biocontrol of Salmonella Enteritidis on Chicken breast meat and Shell eggs using multiphage cocktail.","authors":"Aleena Kokab, Ali Shekh, Masood Rabbani, Wasim Shehzad, Muhammad Ilyas Riaz, Qurat Ul Ain","doi":"10.12834/VetIt.2975.19320.1","DOIUrl":"https://doi.org/10.12834/VetIt.2975.19320.1","url":null,"abstract":"<p><p>The study aimed to evaluate the efficacy of a phage cocktail to reduce Salmonella Enteritidis contamination on perishable food items viz. chicken breast meat and shell eggs using different concentrations. Initially, four bacteriophages €P54, €P59, €P66, and €P72 were isolated from sewage water using Salmonella Enteritidis as a target strain. €P54 and €P66 were found to be Myoviruses while €P59 and €P72 belonged to the Siphoviridae family. A phage cocktail was applied at a concentration of 100 and 10,000 multiplicity of infection (MOI) after artificially contaminating both food items with Salmonella Enteritidis. Results showed that, phage cocktail significantly (p ≤ 0.05) reduced Salmonella Enteritidis count at both concentrations. However, the increased reduction was witnessed at 10,000 MOI. In comparison to untreated control, on chicken breast meat bacterial count was reduced to 1.94 and 3.17 Log10 cfu/g at 100 and 10,000 MOI respectively at 4oC. Similarly, on shell eggs, the bacterial count was reduced to 3.09 and 2.81 Log10 cfu/mL at 10,000 MOI at 4°C and 25°C respectively, while at 100 MOI there was less drop in bacterial count at both 4°C and 25°C. The results showed a better reduction at 4°C as compared to 25°C. Our data showed that the phage cocktail is an effective alternative and additional measure compared to conventional bacterial control methods for meat and eggs.</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141580953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"First molecular detection of shiga toxin-producing Escherichia coli in dogs from serbia: a potential threat to human health?","authors":"Vuk Vračar, Aleksandar Potkonjak, Ljubica Spasojević Kosić, Vesna Lalošević, Gordana Kozoderović","doi":"10.12834/VetIt.2569.18469.2","DOIUrl":"10.12834/VetIt.2569.18469.2","url":null,"abstract":"<p><p>Shiga toxin‑producing Escherichia coli (STEC) are considered one of the most significant E. coli pathotypes transmitted by food, causing life‑threatening conditions in children and elderly people. The aim of this study was to investigate the presence and determine the prevalence of STEC in dogs in Serbia by conventional PCR method, targeting three major virulence genes (stx1, stx2, and eae). The overall percentage of positive samples was 12.87% (13/101), with the stx2 gene, the more potent of the two toxins, found in all the positive samples. The finding of eae gene in combination with stx genes (8/13) within the same genetic pool implies the potential presence of enterohemorrhagic E. coli or the potential emergence of these strains, considering an efficient mechanism of horizontal transfer of three major virulence genes. Our results also highlight dogs' lifestyle as a risk factor for STEC colonisation. These E. coli strains, according to our results, are more likely to be found in dogs living outdoors than those kept in house. Due to significant prevalence of STEC in dogs determined in this research and due to close contact between dogs and humans, dogs could be considered a source of human infections.</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140852248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Michela Quaglia, Cipriano Foxi, Giuseppe Satta, Giantonella Puggioni, Robero Bechere, Matteo De Ascentis, Silvio Gerardo D'Alessio, Massimo Spedicato, Alessandra Leone, Maura Pisciella, Ottavio Portanti, Liana Teodori, Luigina Di Gialleonardo, Cesare Cammà, Giovanni Savini, Maria Goffredo
{"title":"Culicoides species responsible for the transmission of Epizootic Haemorrhagic Disease virus (EHDV) serotype 8 in Italy.","authors":"Michela Quaglia, Cipriano Foxi, Giuseppe Satta, Giantonella Puggioni, Robero Bechere, Matteo De Ascentis, Silvio Gerardo D'Alessio, Massimo Spedicato, Alessandra Leone, Maura Pisciella, Ottavio Portanti, Liana Teodori, Luigina Di Gialleonardo, Cesare Cammà, Giovanni Savini, Maria Goffredo","doi":"10.12834/VetIt.3347.22208.1","DOIUrl":"10.12834/VetIt.3347.22208.1","url":null,"abstract":"<p><p>Epizootic haemorrhagic disease (EHD) is a viral disease transmitted by Culicoides biting midges that affects wild and domestic ruminants. The causative agent, EHD virus (EHDV), belongs to the family Sedoreoviridae, genus Orbivirus. The virus has never been reported in Europe until October 2022, when the virus was for the first time detected in Sicily and Sardinia. After the first clinical cases, an intensive entomological field activity was carried out in five EHD affected farms located in Sardinia, with the aim of assessing the EHDV vector competence in European species of Culicoides. EHDV‑8 was detected in C. imicola, C. obsoletus/scoticus, C. newsteadi, C. pulicaris ss, and C. bysta. The first 4 species have also been demonstrated to be able to transmit bluetongue virus (BTV). According to these results, it is likely that EHDV‑8, sharing the same transmission patterns of BTV, can also spread to Europe.</p>","PeriodicalId":23550,"journal":{"name":"Veterinaria italiana","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41138067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}