Veterinary Parasitology: X最新文献

{"title":"Trichinella spiralis - New method for sample preparation and objective detection of specific antigens using a chemiluminescence immunoassay.","authors":"Jana Braasch, Stefanie Ostermann, Monika Mackiewicz, Catherine Bardot, Caroline Pagneux, Viola Borchardt-Lohölter, Erik Lattwein","doi":"10.1016/j.vpoa.2020.100033","DOIUrl":"https://doi.org/10.1016/j.vpoa.2020.100033","url":null,"abstract":"<p><p>The parasitic roundworm <i>Trichinella spiralis</i> is most commonly transmitted to humans through consumption of raw or undercooked meat of infected pigs or game. To prevent human infection, slaughterhouses perform meat safety surveillance using the gold standard \"Magnetic Stirrer Method\". We introduce a fast and objective method using automated detection of specific <i>Trichinella spiralis</i> antigens by a newly developed immunoassay based on chemiluminescence (ChLIA). Panel A comprised muscle tissue samples from non-infected pigs (n = 37). Panel B comprised muscle tissue samples from non-infected pigs spiked with different amounts of <i>Trichinella</i> larvae without collagen capsules (n = 56). Panel C contained muscle tissue samples from experimentally infected pigs including <i>Trichinella</i> larvae encapsulated in collagen (n = 32). Each sample was shredded with PBS buffer in a knife mill, destroying <i>Trichinella</i> larvae. Following centrifugation, the supernatant (muscle tissue extract containing released excretory and secretory <i>Trichinella spiralis</i> antigens) was used for <i>Trichinella-</i>specific antigen detection by the new Trichinella ChLIA. The overall accuracy of the Trichinella ChLIA was 97.6 %. The specificity of the Trichinella ChLIA was 100 % (panel A). The sensitivity in samples from experimentally infected pigs was 100 % representing a detection limit of 0.01 larvae per gram. Cross-reactivity with parasites other than <i>Trichinella spp</i>. was not observed. This new meat inspection method for the detection of <i>Trichinella spiralis</i> antigens presents high specificity and high sensitivity, especially in truly infected samples. In contrast to the gold standard, this new approach to meat safety surveillance does not require longsome digestion or microscopy by trained personnel.</p>","PeriodicalId":23537,"journal":{"name":"Veterinary Parasitology: X","volume":"4 ","pages":"100033"},"PeriodicalIF":0.0,"publicationDate":"2020-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7711141/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141890240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potentials of using milk performance data and FAMACHA score as indicators for Targeted Selective Treatment in Lacaune dairy sheep in Switzerland.","authors":"Katharina Schwarz, Beat Bapst, Mirjam Holinger, Susann Thüer, Inga Schleip, Steffen Werne","doi":"10.1016/j.vpoa.2020.100030","DOIUrl":"10.1016/j.vpoa.2020.100030","url":null,"abstract":"<p><p>Targeted Selective Treatment (TST) is one approach to slow down the development of anthelmintic resistance. Its success is closely linked to the correct identification of animals in need of treatment. In dairy goats it has been proposed to use milk yield as TST indicator and to focus treatments on high yielding dairy goats. In dairy sheep the relationship between milk performance and infection with gastrointestinal nematodes (GIN) is not well known. The aim of this study was to investigate the relationship between milk yield and GIN infection in dairy sheep and based on this, to evaluate milk performance data as a potential TST indicator. Overall 1159 Lacaune ewes of 15 dairy sheep farms in Switzerland were included in the study. The ewes were phenotyped once between August and December 2019, when they were at least 70 days in milk (DIM). Individual faecal samples were taken from every ewe to determine the nematode egg concentration per gram faeces (EPG). In addition, the clinical parameters FAMACHA score and packed cell volume (PCV) were measured. Linear mixed models were used to analyse the effects of the collected parameters on EPG. EPG increased significantly with increasing test day milk yields (P = 0.002), indicating high yielding ewes to be less resistant to GIN infections than low yielding ewes. The effect was most pronounced in earlier lactation but remained within a moderate range. Overall, our results indicated the potential of using milk yield data of rather early lactation as TST indicator in dairy sheep. On farms with predominantly <i>H. contortus</i> the combination with FAMACHA might improve the correct identification of highly infected ewes, as FAMACHA was correlated with EPG (r = 0.37, P < 0.001).</p>","PeriodicalId":23537,"journal":{"name":"Veterinary Parasitology: X","volume":"4 ","pages":"100030"},"PeriodicalIF":0.0,"publicationDate":"2020-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7494794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140319282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-home assessment of flea control and dermatologic lesions in dogs provided by lotilaner (Credelio^®) and spinosad (Comfortis^®) in west central Florida.","authors":"Michael W Dryden, Michael S Canfield, Brian H Herrin, Cara Bocon, Todd S Bress, Anna Hickert, Todd M Kollasch, Letitia Phan, Anthony J Rumschlag, William G Ryan, Bridgette Sampeck, Nicole Smith, Vicki Smith, Stanislaw A Warcholek","doi":"10.1016/j.vpoa.2019.100009","DOIUrl":"https://doi.org/10.1016/j.vpoa.2019.100009","url":null,"abstract":"<p><p>Post-launch field investigations of recently-approved flea control products establish an efficacy baseline and in subsequent years can detect any efficacy decline suggestive of emerging resistance. As part of a continuing program of yearly assessment of flea control products in west central Florida, this study, using client-owned dogs, investigated the efficacy of lotilaner and spinosad in controlling fleas and in alleviating dermatologic signs likely associated with flea infestations. Forty-four qualifying households were randomized to either a lotilaner (Credelio^®) (minimum dose rate 20 mg/kg) or a spinosad (Comfortis^®) (30 mg/kg) group, with 33 and 36 dogs in each group, respectively. On Days 0 and 28 (±2) all dogs in each household were treated with the allocated product according to label directions, and all household cats received spinetoram (Cheristin^®). On Day 0 and at weekly intervals through Day 56 (±2), on-animal and premises flea burdens were enumerated, a veterinary dermatologist scored integumental changes using canine atopic dermatitis extent and severity index (CADESI)-4 and flea allergy dermatitis (FAD) scales, and owners scored pruritus using the validated canine pruritus severity scale (CPSS). At study entry geometric mean flea counts were 33.2 and 29.9 in the lotilaner and spinosad groups, respectively. For both groups, reductions in flea counts were > 99% at the first post-treatment assessment (Week 1), and 100% from Week 6 through the final assessment (Week 8) when all study dogs were flea-free. For both groups, at each timepoint, flea counts on dogs and in traps were significantly reduced compared to the initial assessment (p < 0.001), as were improvements in median CADESI-4, FAD and CPSS scores (p ≤ 0.001). At Week 4, the geometric mean flea count on dogs in the lotilaner group (0.1) was significantly lower than that of dogs in the spinosad group (0.6) (p = 0.027), significantly fewer dogs in the lotilaner group were found to have fleas (p = 0.034), and mean owner-rated pruritus scores were significantly lower (p = 0.025). Under field conditions favoring heavy flea challenge, two consecutive monthly treatments of dogs with either lotilaner or spinosad produced a 100% reduction in canine flea infestations and dramatic improvements in dermatologic lesions and pruritus, based on scoring by a veterinary dermatologist and by dog owners. Household flea burdens were driven to extinction in all but one home in each treatment group.</p>","PeriodicalId":23537,"journal":{"name":"Veterinary Parasitology: X","volume":"1 ","pages":"100009"},"PeriodicalIF":0.0,"publicationDate":"2019-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7458372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141861073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of an injectable toltrazuril - gleptoferron (Forceris^®) to control coccidiosis (<i>Cystoisospora suis</i>) in comparison with iron supplemented piglets without anticoccidial treatment.","authors":"Lysanne Hiob, Ivette Holzhausen, Daniel Sperling, Gaëlle Pagny, Laurianne Meppiel, Naomi Isaka, Arwid Daugschies","doi":"10.1016/j.vpoa.2019.100002","DOIUrl":"https://doi.org/10.1016/j.vpoa.2019.100002","url":null,"abstract":"<p><p>The efficacy and safety of an injectable combination product containing toltrazuril and gleptoferron (Forceris^® - CEVA) for the control of coccidiosis due to <i>Cystoisospora suis</i> was investigated in neonatal piglets. The study was carried out on five European commercial pig farms in France, Germany and Spain and 122 litters consisting of 1508 piglets were selected and randomly allocated to one of two treatment groups. The first group received a single intramuscular injection per piglet of the test product, containing 45 mg toltrazuril and 200 mg iron and this was compared with a control group, which was administered a single intramuscular treatment of iron at 200 mg per piglet only. Body weights, faecal scores and oocysts counts, recorded as oocysts per gram of faeces, (OPG) were observed for 21 days. Only 1138 piglets were actually exposed to coccidiosis and data sets of these animals were selected for statistical analysis. The efficacy of the test product in the control of coccidiosis was shown in higher body weight gains, a lower percentage of animals with diarrhoea, fewer samples with positive oocysts counts as well as a lower excretion peak and a reduced area under the curve of OPG from study day (SD) 4 - SD 21. The combination product of toltrazuril and gleptoferron provided an effective alternative approach to current conventional separate treatment for the prevention of iron deficiency anaemia and coccidiosis in neonatal piglets. It reduced the numbers of potentially stressful interventions and work time.</p>","PeriodicalId":23537,"journal":{"name":"Veterinary Parasitology: X","volume":"1 ","pages":"100002"},"PeriodicalIF":0.0,"publicationDate":"2019-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7458377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140319281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative assessment of faecal diagnostics for detection and predictive modelling of endemic <i>Fasciola hepatica</i> infection in sheep and cattle on Australian farms.","authors":"S D George, A J George, P F Rolfe, D L Emery","doi":"10.1016/j.vpoa.2018.100001","DOIUrl":"10.1016/j.vpoa.2018.100001","url":null,"abstract":"<p><p>The diagnosis, monitoring and flukicide efficacy testing of fasciolosis on-farm is reliant on non-terminal methods. The coproantigen ELISA (cELISA) has been recommended for diagnosis of fasciolosis and associated flukicide efficacy testing as an alternative to fluke egg counts for monitoring parasitism. Recently experimental multi-age infections have suggested that the reliability of efficacy results can be improved by a second cELISA testing at 6 weeks post-treatment (wpt) in addition to the generally accepted 1 wpt. A field study was conducted to determine the suitability of faecal fluke egg counts (FFEC) and cELISA as diagnostic, drug efficacy testing and epidemiological tools on Australian sheep and cattle farms. Faecal samples from sheep and/or cattle on three endemic farms were taken at monthly intervals for 12 months and examined by both methods. Normal farm management was maintained during the study period and opportunistic efficacy testing, in line with each farm's normal flukicide management was undertaken. Additionally, the suitability of the Ollerenshaw Index as a predictive model for fasciolosis under Australian conditions was examined. While both diagnostics demonstrated their value in the farm environment, the current data demonstrate a distinct and significant increase in diagnostic sensitivity for epidemiological studies by using the two tests in parallel. The agreement between the two diagnostics was found to be higher in cattle, despite the poor sensitivity of FFEC in this species. Similar levels of agreement between the two tests were demonstrated at both sheep properties, regardless of the marked difference in the intensity of <i>F. hepatica</i> challenge. Linear regression models demonstrated the results of the two diagnostics utilized in parallel were explained substantially (R² = 0.91) as were series data (R² = 0.88) when the respective models were fitted. In contrast, the fitted models for FFEC (R² = 0.54) and cELISA (R² = 0.58) were poor explanations for test outcomes. The outcomes of these models support previous findings that suggest that the two diagnostic tests are best utilized together, particularly in parallel. The application of the Ollerenshaw Index to Australian conditions requires further investigation.</p>","PeriodicalId":23537,"journal":{"name":"Veterinary Parasitology: X","volume":"1 ","pages":"100001"},"PeriodicalIF":0.0,"publicationDate":"2019-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7458369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140319352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}