Voprosy meditsinskoi khimii最新文献_第4页

[Levels of autoantibodies to glutamate receptors and immunological blood parameters in patients with epilepsy]. [癫痫患者谷氨酸受体自身抗体水平和免疫血液参数]。

Voprosy meditsinskoi khimii Pub Date : 2002-07-01

V G Bazarova, O K Granstrem, S A Dambinova

引用次数: 0

[Correction of lipid metabolism in rabbits with experimental cholelithiasis]. [对实验性胆石症家兔脂质代谢的纠正]。

Voprosy meditsinskoi khimii Pub Date : 2002-07-01

Iu A Bogdarin, D V Kozlov

引用次数: 0

[Mitochondrial diseases--a new branch of the modern medicine]. [线粒体疾病——现代医学的一个新分支]。

Voprosy meditsinskoi khimii Pub Date : 2002-07-01

P P Zagoskin, E M Khvatova

{"title":"[Mitochondrial diseases--a new branch of the modern medicine].","authors":"P P Zagoskin, E M Khvatova","doi":"","DOIUrl":"","url":null,"abstract":"The review highlights current aspects of a large group of diseases the main pathogenetic element of which is an inherited or acquired disturbance of gene expression of nuclear or mitochondrial genome encoding mitochondrial proteins. The recent data on mutant genetic loci specific to the most wide spread mitochondrial diseases are considered. The steps of pathogenesis, include the mutations of nuclear or mitochondrial genes, disturbances of mitochondrial protein synthesis, dissipation of proton membrane potential, opening of a permeability transition pore, releasing of procaspases, cytochrome c, and other proapoptotic molecules, and finally chromatin fragmentation and apoptotic cell death. We discuss the possible reasons of polysymptomatic character and different variants of mitochondrial disease manifestations on the basis of the phenomenon of mitochondrial DNA heteroplasmy and metabolic compensation of the genetic defects. Modern biochemical methods of a mitochondrial disease diagnostics: (PCR-amplification, polarographic research of mitochondrial respiration and oxidative phosphorylation, analysis and monitoring of metabolites in biological liquids) are characterized. The basic principles and perspectives of the treatment of mitochondrial diseases, (gene therapy, correction of metabolic disorders, application of antioxidants and neuropeptides) are described.","PeriodicalId":23535,"journal":{"name":"Voprosy meditsinskoi khimii","volume":"48 4","pages":"321-36"},"PeriodicalIF":0.0,"publicationDate":"2002-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22177333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Evaluation of preservation and the collagen structure state in the mummy tissues]. [木乃伊组织中胶原蛋白结构状态及保存评价]。

Voprosy meditsinskoi khimii Pub Date : 2002-07-01

Iu A Romakov, V A Bykov, G A Rebrova, V K Vasilevskiĭ, So San Ho, L B Rebrov

引用次数: 0

[All-Russian Conference "Problems of Medicinal Enzymology. Modern Technologies in Laboratory Diagnosis in the New Century". Moscow. May, 2002. Abstracts]. 全俄会议“药物酶学问题”新世纪实验室诊断的现代技术”。莫斯科。2002年5月。摘要]。

Voprosy meditsinskoi khimii Pub Date : 2002-07-01

引用次数: 0

[Genetic heterogeneity of cytochrome CYP51 in clinical strains of Mycobacterium tuberculosis]. [临床结核分枝杆菌菌株细胞色素CYP51的遗传异质性]。

Voprosy meditsinskoi khimii Pub Date : 2002-07-01

A S Shavkunov, V N Lazarev, L N Chernausova, A V Kuz'min, V M Govorun

{"title":"[Genetic heterogeneity of cytochrome CYP51 in clinical strains of Mycobacterium tuberculosis].","authors":"A S Shavkunov, V N Lazarev, L N Chernausova, A V Kuz'min, V M Govorun","doi":"","DOIUrl":"","url":null,"abstract":"Information on the complete genome sequences of a number of organisms available recently offers essentially new opportunities for the development of new, highly effective antimicrobial compounds. In particular, the search for new effective antituberculosis drugs remains an important problem, due to the recent increase of number of patients suffering with tuberculosis. In this respect considerable attention is paid to the cyp51-like gene Rv0764c encoding sterol-14 alpha-demethylase belonging to the cytochrome P450 superfamily, which has been discovered by computer analysis of the Mycobacterium tuberculosis genome sequence. We have screened 64 clinical isolates of M. tuberculosis for functionally relevant mutations in the coding sequence of the gene encoding Cyp 51-demethylase by single-strand conformation polymorphism analysis (SSCP) and sequencing of PCR-amplified gene fragments. Structural analysis of the gene in the isolates revealed no mutations leading to amino acid substitutions in the corresponding protein. 10 isolates had a silent nucleotide substitution 114 GCT-->GCC. Computer analysis of cyp51 sequence of the CDC1551 strain also revealed a similar nucleotide substitution, which has not been mentioned previously. The data obtained demonstrate that the sequence of the gene is highly conserved, supporting the advisability of M. tuberculosis Cyp51 protein to be considered as a molecular target for new antitubercular drugs. The SNP found in the gene coding sequence may be employed in the studies of M. tuberculosis population genetics.","PeriodicalId":23535,"journal":{"name":"Voprosy meditsinskoi khimii","volume":"48 4","pages":"361-7"},"PeriodicalIF":0.0,"publicationDate":"2002-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22177146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Myeloperoxidase in the human placenta at preterm labor]. [早产儿人胎盘中的髓过氧化物酶]。

Voprosy meditsinskoi khimii Pub Date : 2002-07-01

V M Prokopenko, G M Aleshina, E V Frolova, V V Anan'eva, V N Kokriakov, A V Arutiunian

引用次数: 0

[Regulation of ceruloplasmin activity in oncogynecological diseases]. [妇科肿瘤中铜蓝蛋白活性的调控]。

Voprosy meditsinskoi khimii Pub Date : 2002-07-01

Iu N Gusarova, E V Stepanova, E O Landesman, O V Koroleva, T P Vavilova, O V Makarov, V N Kosetskiĭ

引用次数: 0

[A drop of mink complement kills a mouse]. [一滴水貂补剂杀死一只老鼠]。

Voprosy meditsinskoi khimii Pub Date : 2002-07-01

Z P Belkin, L V Kozlov, S S Andina, A A Mishin, V A Guzova, V L D'iakov

引用次数: 0

[Effect of pyruvate, threonine, and phosphoethanolamine on acetaldehyde metabolism in rats with toxic liver injury]. [丙酮酸、苏氨酸和磷酸乙醇胺对中毒性肝损伤大鼠乙醛代谢的影响]。

Voprosy meditsinskoi khimii Pub Date : 2002-05-01

P S Pron'ko, V I Satanovskaia, B I Gorenshteĭn, A B Kuz'mich, T N Pyzhik

{"title":"[Effect of pyruvate, threonine, and phosphoethanolamine on acetaldehyde metabolism in rats with toxic liver injury].","authors":"P S Pron'ko, V I Satanovskaia, B I Gorenshteĭn, A B Kuz'mich, T N Pyzhik","doi":"","DOIUrl":"","url":null,"abstract":"Pyruvate dehydrogenase, threonine aldolase and phosphoethanolamine lyase can produce acetaldehyde during normal metabolism. We studied the effect of loading with the substrates of these enzymes (pyruvate, 500 mg/kg, i.p., threonine 500 mg/kg, i.p., and phosphoethanolamine, 230 mg/kg, i.p.) on the blood concentrations of endogenous acetaldehyde and ethanol and the activities of enzymes producing and oxidizing acetaldehyde in the liver of normal rats and rats with liver injury provoked by chronic carbon tetrachloride (CCl4) treatment (0.2 ml i.p. per rat, 2 times a week during 4 weeks). Blood was collected before the treatment and then 30 min and 1 h following the administration of the substrates to intact and CCl4-treated rats. Endogenous acetaldehyde and ethanol were determined by headspace GC. The CCl4 treatment resulted in decreased liver alcohol dehydrogenase and aldehyde dehydrogenase activities and a significant elevation of liver endogenous ehtanol and a clear tendency to enhance blood acetaldehyde levels. Pyruvate increased blood endogenous acetaldehyde in CCl4-treated animals and endogenous ethanol--in the control group of animals. Threonine elevated endogenous acetaldehyde in normal rats. Phosphoethanolamine increased endogenous ethanol in the intact and CCl4 groups. At the same time, in CCl4-treated rats pyruvate administration increased the liver pyruvate dehydrogenase, threonine decreased threonine aldolase, whereas phosphoethanolamine decreased phosphoethanolamine lyase. Thus, the CCl4 effect on blood endogenous acetaldehyde and ethanol may be mediated through decreased liver ALDH and ADH activities. Liver injury promotes the accumulation of acetaldehyde, derived from physiological sources, including the degration of pyruvate and threonine by decreased acetaldehyde oxidation.","PeriodicalId":23535,"journal":{"name":"Voprosy meditsinskoi khimii","volume":"48 3","pages":"278-85"},"PeriodicalIF":0.0,"publicationDate":"2002-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22000712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0