Virchows Archiv. B, Cell pathology including molecular pathology最新文献_第3页

Repressed activity of peritoneal macrophages in methimazole-induced hypothyroid mice. 甲巯咪唑诱导甲状腺功能减退小鼠腹膜巨噬细胞活性的抑制。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02899252

W K Liu, K W Tsui, C C Wong

引用次数: 14

Current pathogenetic and molecular concepts in viral liver carcinogenesis. 目前病毒性肝癌的发病机制和分子概念。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02899246

P Schirmacher, C E Rogler, H P Dienes

引用次数: 41

A novel role in the removal of blood-borne foreign bodies for pulmonary capillaries in the guinea pig. 豚鼠肺毛细血管血源性异物清除中的新作用。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02899255

M Terada

{"title":"A novel role in the removal of blood-borne foreign bodies for pulmonary capillaries in the guinea pig.","authors":"M Terada","doi":"10.1007/BF02899255","DOIUrl":"https://doi.org/10.1007/BF02899255","url":null,"abstract":"In certain mammals, the lung plays an important role in removing blood-borne foreign bodies by means of the numerous macrophages disposed in pulmonary capillaries. The present ultrastructural study demonstrates that in the guinea pig, the lung also plays a significant role in this respect, but that in this species, foreign body elimination takes place by another, hitherto undescribed process. In the guinea pig, the pulmonary capillaries are characterized by numerous neutrophils that adhere to endothelial cells even under normal conditions. At 30 min after intravenous injection of glutaraldehyde-fixed erythrocytes, large numbers of these foreign bodies were found to be ingested by these neutrophils. At 6 h after injection, the erythrocyte-carrying neutrophils had disappeared from the vascular lumen, but endothelial cells had begun vigorously to engulf the fixed erythrocytes by extending membranous processes which completely enwrapped them. Since endothelial cells lack lysosomes, there was no morphologic evidence of erythrocyte digestion within their cytoplasm. It is evident that the erythrocytes then passed through the endothelium, since by 48 h after injection, most of them were incorporated and digested by macrophages proliferating in the alveolar interstitium. The increase in macrophages was confirmed by acid phosphatase histochemistry. These observations indicate that in the guinea pig, the lung plays an important role in clearing blood-borne foreign bodies by the sequential involvement of intracapillary neutrophils, capillary endothelial cells and interstitial macrophages.","PeriodicalId":23521,"journal":{"name":"Virchows Archiv. B, Cell pathology including molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02899255","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19089812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Heparins modulate extracellular matrix and protein synthesis of cultured rat mesangial cells. 肝素调节培养大鼠系膜细胞的细胞外基质和蛋白合成。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02899259

A Wolthuis, A Boes, J H Berden, J Grond

{"title":"Heparins modulate extracellular matrix and protein synthesis of cultured rat mesangial cells.","authors":"A Wolthuis, A Boes, J H Berden, J Grond","doi":"10.1007/BF02899259","DOIUrl":"https://doi.org/10.1007/BF02899259","url":null,"abstract":"Heparins blunt the development of glomerulosclerosis in several disease models in the rat and this protective effect may be related to suppression of glomerular cell proliferation. In this study the direct effect of heparins on another key event in glomerulosclerosis, extracellular matrix (ECM) deposition, was examined. Standard heparin (hep) and non-anticoagulant N-desulfated acetylated heparin (DSA-hep) significantly reduced the fibronectin content in the conditioned media of subconfluent, confluent, and supraconfluent rat glomerular mesangial cells (MCs) in culture, as assessed by a sandwich ELISA technique. Both heparins significantly increased the amount of cell-associated fibronectin in sparse and subconfluent MCs. DSA-hep, but not hep, increased the fibronectin content of ECM formed by confluent and supraconfluent MCs. Using 3H-proline pulse-labeling, Hep and DSA-hep were found to significantly decrease cell-associated collagen in subconfluent but not in confluent MCs. No effects were seen on newly synthesized collagen secreted into the culture medium. Neither hep nor DSA-hep affected total protein synthesis, studied by metabolic labeling with 35S-methionine. High resolution 2-D electrophoresis (molecular weight range, 120 to 10 Kd; isoelectric interval, 5.0 to 7.0) revealed one particular intracellular protein (molecular weight 54 Kd, pI 5.91) which was consistently overexpressed in hep. Both heparins affected an identical set of another 19 different intracellular MC proteins (over-/underexpression or shift to higher molecular weights). In conclusion, the present data demonstrate the profound direct metabolic effects of hep and DSA-hep.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":23521,"journal":{"name":"Virchows Archiv. B, Cell pathology including molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02899259","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19089816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Fetal rat pancreas transplantation in BB rats: immunohistochemical and functional evaluation. BB大鼠胎鼠胰腺移植:免疫组织化学和功能评价。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02915091

K B Yderstraede, H Starklint, D Steinbruchel, T W Jørgensen, C F Gotfredsen

{"title":"Fetal rat pancreas transplantation in BB rats: immunohistochemical and functional evaluation.","authors":"K B Yderstraede, H Starklint, D Steinbruchel, T W Jørgensen, C F Gotfredsen","doi":"10.1007/BF02915091","DOIUrl":"https://doi.org/10.1007/BF02915091","url":null,"abstract":"Spontaneously diabetic BB/Wor rats received either a syngeneic fetal pancreas transplant or adult islets. In the former, 4-8 fetal pancreases were transplanted, and in the latter, 3-5000 islets. Transplantation was performed by transferring a blood clot containing the pancreases or islets to the renal subcapsular space. Insulin therapy was undertaken postoperatively, except in one experiment with adult islets. Of the fetal pancreas transplanted BB rats, 52% became normoglycaemic, and 21% remained so throughout an observation period of 10 months. Nephrectomy caused a prompt return of diabetes. The histological appearance of the grafts transplanted to the diabetic animals closely resembled that of grafts transplanted to normal rats in a parallel series. For comparison a group of BB rats received a syngeneic transplant of isolated adult islets from WF rats or BBW rats. Following adult islet transplantation, 5 out of 6 animals became hyperglycaemic after a median of 20.5 days when no insulin was given post-transplantation. Four out of 5 animals became hyperglycaemic after a median of 23 days when supportive insulin therapy was administered after the transplantation. The results indicate that recurrent diabetes is not inevitable following syngeneic fetal pancreas transplantation to spontaneously diabetic BB rats. Recurrent diabetes was only occasionally associated with mononuclear cell infiltration. Transplanted tissue was well-preserved and vascularized; mega-islets were a constant finding.","PeriodicalId":23521,"journal":{"name":"Virchows Archiv. B, Cell pathology including molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02915091","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19385801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Smooth muscle differentiation in human myometrium and uterine leiomyoma. 人子宫肌层与子宫平滑肌瘤的平滑肌分化。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02915092

A Draeger, A H Graf, A Staudach, A J North, J V Small

引用次数: 13

Morphological characterization of a new human epithelioid sarcoma cell line, ES020488, in vitro and in vivo. 一种新的人上皮样肉瘤细胞系ES020488的体外和体内形态学特征

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02899265

H Sonobe, M Furihata, J Iwata, T Oka, Y Ohtsuki, S Hamasato, S Fujimoto

{"title":"Morphological characterization of a new human epithelioid sarcoma cell line, ES020488, in vitro and in vivo.","authors":"H Sonobe, M Furihata, J Iwata, T Oka, Y Ohtsuki, S Hamasato, S Fujimoto","doi":"10.1007/BF02899265","DOIUrl":"https://doi.org/10.1007/BF02899265","url":null,"abstract":"A new human epithelioid sarcoma cell line (ES020488) was established from a cutaneous metastasis in 26-year-old man, and was morphologically characterized in vitro and in vivo by comparison with the original tumor. The ES020488 cells showed a male karyotype ranging from 39 to 83 chromosomes, with various abnormalities but no specific pattern. The cells were round, polygonal or spindle-shaped with abundant cytoplasm and round nuclei containing prominent nucleoli; they proliferated in a sheet-like pattern. Tumors transplanted into nude mice revealed essentially the same features as the original tumor. Both in vitro and in vivo, the cells immunohistochemically expressed vimentin, cytokeratin, and EMA, but not desmin and S-100 protein. Ultrastructural study revealed irregular or round nuclei containing abundant euchromatin and prominent nucleoli, many intermediate filaments running irregularly or around the nucleus, and a number of filopodia-like processes. ES020488 cells were thus proven to retain and exhibit the unique morphological characteristics of an epithelioid sarcoma both in vitro and in vivo. These cells are possibly derived from synovioblastic mesenchyme.","PeriodicalId":23521,"journal":{"name":"Virchows Archiv. B, Cell pathology including molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02899265","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18689718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Relationship between quantity of silver stained nucleolar organizer region associated proteins (Ag-NORs) and growth rate suppression of breast cancer cell lines after interferon-gamma and 4-hydroxy-tamoxifen treatment. 银染色核仁组织区相关蛋白(Ag-NORs)数量与干扰素- γ和4-羟基他莫昔芬治疗后乳腺癌细胞株生长速率抑制的关系

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02899284

D Ofner, A Hittmair, C Marth, M Tötsch, G Daxenbichler, R Margreiter, W Böcker, K W Schmid

{"title":"Relationship between quantity of silver stained nucleolar organizer region associated proteins (Ag-NORs) and growth rate suppression of breast cancer cell lines after interferon-gamma and 4-hydroxy-tamoxifen treatment.","authors":"D Ofner, A Hittmair, C Marth, M Tötsch, G Daxenbichler, R Margreiter, W Böcker, K W Schmid","doi":"10.1007/BF02899284","DOIUrl":"https://doi.org/10.1007/BF02899284","url":null,"abstract":"The correlation between the silver-stained nucleolar organizer region associated proteins (Ag-NORs) and the growth rate suppression (GRS) of ten established breast cancer cell lines which were treated with 4-hydroxy-tamoxifen (OHT) and interferon-gamma (g-IFN), respectively, was investigated by means of automated image analysis. Previous studies have shown a statistically significant relationship between the Ag-NOR quantity and the population doubling time (PDT) of these cell clones. The results of the present study showed a highly significant correlation between the GRS and the Ag-NOR quantity in estrogen receptor (ER) positive tumour cell lines after OHT treatment (P < 0.001) whereas no strict correlation of these parameters could be demonstrated after g-IFN treatment in both ER positive and negative cell lines. Our results suggest a different behaviour of NOR-proteins in breast cancer cell lines if treated either with g-IFN or OHT, probably reflecting the different mechanism of cell suppression mediated by OHT and g-IFN. It is concluded that quantitative assessment of Ag-NORs is not as suitable for the determination of the GRS as it is for the determination of cell duplication rates obtained on untreated tumour cell lines.","PeriodicalId":23521,"journal":{"name":"Virchows Archiv. B, Cell pathology including molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02899284","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19093446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Apoptosis and cell desquamation in repair process of ischemic tubular necrosis. 缺血性小管坏死修复过程中的细胞凋亡和细胞脱屑。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02915110

A Shimizu, N Yamanaka

{"title":"Apoptosis and cell desquamation in repair process of ischemic tubular necrosis.","authors":"A Shimizu, N Yamanaka","doi":"10.1007/BF02915110","DOIUrl":"https://doi.org/10.1007/BF02915110","url":null,"abstract":"To elucidate the role of apoptosis and cell desquamation in the repair phase of acute tubular necrosis, morphological findings after 60 min ischaemia were investigated in rats. A morphometric analysis of the cell proliferation and of the epithelial cellularity of reconstructing tubules was performed. The kinetics of apoptosis and cell desquamation were also examined. Ischaemia and reperfusion injury resulted in widespread necrosis of tubules at day 1. Subsequently, a regenerative epithelial hyperplasia took place in the early stage. The most marked increase in cellularity in the damaged tubules was on day 6, when the tubules became lined by hyperplastic epithelial cells with papillary clusters. The number of papillary clusters decrease up to day 8, and during this period many desquamated cells from the clusters were observed in the tubular lumen. In the later stage, hyperplastic epithelial cells were reduced to their original cellularity and during this period the number of apoptotic cells obviously increased, while the damaged tubules were reconstructed. We conclude that epithelial overproduction occurs in the early phase after tubular necrosis, and excess hyperplastic epithelial cells regress during the repair process by cell desquamation and apoptosis, both of which are essential for the recovery of the original tubular structure.","PeriodicalId":23521,"journal":{"name":"Virchows Archiv. B, Cell pathology including molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02915110","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19231483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 138

Characterization of the inflammatory cell populations in normal colon and colonic carcinomas. 正常结肠癌和结肠癌中炎性细胞群的特征。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02915115

B F Banner, L Savas, S Baker, B A Woda

{"title":"Characterization of the inflammatory cell populations in normal colon and colonic carcinomas.","authors":"B F Banner, L Savas, S Baker, B A Woda","doi":"10.1007/BF02915115","DOIUrl":"https://doi.org/10.1007/BF02915115","url":null,"abstract":"Unlabelled: Little is known about the nature of the mucosa-associated immune system within the normal colon, or about the immune response to colon carcinoma. In this study inflammatory cells (ICs) in 14 normal colons and 14 carcinomas were characterized. Overall inflammation, lymphocytes, plasma cells, neutrophils, and eosinophils were graded in routine H & E sections. Frozen sections were stained by an immunoperoxidase technique using antibodies to the T cell associated antigens CD2, CD7, CD4, CD8, and T cell receptors alpha beta and gamma delta. B cells were identified with CD20, macrophages with CD68, and Class II antigen with anti-HLA DR. Each cell type was semiquantitatively graded in 10 high power fields (HPFs) in the lumenal half (LH) or basal half (BH) of the normal mucosae, and in epithelium or stroma of the carcinomas. In normal colons, ICs were more frequent in LH than in BH. Plasma cells, lymphocytes and monocytes predominated. Subtyping of lymphocytes showed that CD4+ TCR alpha beta + T lymphocytes were most numerous in the lamina propria. Lymphocytes within the epithelium were CD8+ T cells. Around carcinomas the overall grade of ICs was 1+ in the majority of cases. Plasma cells, CD4+ and CD8+ cells with the TCR alpha beta receptor, and macrophages were most frequent. Lymphoid aggregates of both T and B cells were frequent.Conclusions: 1. Normal colon contains a diffuse luminally oriented population of TCR alpha beta+ CD4+ T cells, plasma cells, macrophages and class II antigen-expressing cells in the lamina propria.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":23521,"journal":{"name":"Virchows Archiv. B, Cell pathology including molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02915115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19273959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32