Virchows Archiv. B, Cell pathology including molecular pathology最新文献_第2页

Tenascin in inflammatory conditions and neoplasms of the urinary bladder. 膀胱炎症和肿瘤中的腱素。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02899274

O Tiitta, T Wahlström, I Virtanen, V E Gould

{"title":"Tenascin in inflammatory conditions and neoplasms of the urinary bladder.","authors":"O Tiitta, T Wahlström, I Virtanen, V E Gould","doi":"10.1007/BF02899274","DOIUrl":"https://doi.org/10.1007/BF02899274","url":null,"abstract":"Tenascin (Tn) is an extracellular matrix (ECM) glycoprotein strongly and widely expressed during embryogenesis. Tn is decreased in normal adult tissues but is reexpressed in numerous inflammatory, reparative and neoplastic processes. We immunostained samples of fetal and normal adult bladders and samples of bladder tissue from patients with chronic cystitis, detrusor hypertrophy, malakoplakia and transitional cell carcinomas (TCC) of all grades, with a monoclonal antibody (mAb) to Tn 143DB7. Sections of flat in situ carcinomas were also studied. In fetal bladders, strong and ragged Tn reactions were noted at the epithelial-stromal interface; in normal adult bladders, the reaction was delicate and less extensive. In chronic cystitis, Tn reactivity was enhanced particularly around prominent capillary blood vessels. In flat in situ carcinomas, Tn staining was stronger and more extensive than in normal mucosa but was often less extensive than in some examples of cystitis. In TCC I and II, Tn immunoreactivity was strong and predominated in the pericapillary stroma of the papillae; in infiltrating TCC II, comparatively limited Tn staining was noted. In deeply infiltrating grade III TCC with abundant stroma, Tn reaction was invariably strong and extensive, particularly around advancing tumor nests. The strongest Tn reactions were noted in invasive, high-grade TCC with abundant stroma. We conclude that in inflammatory-reactive processes, and in in situ carcinomas as well as in TCC, the extent and intensity of the Tn reaction correlates with the severity of the inflammatory infiltrate and with the extent of the stromal remodelling.","PeriodicalId":23521,"journal":{"name":"Virchows Archiv. B, Cell pathology including molecular pathology","volume":"63 5","pages":"283-7"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02899274","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18688735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 36

p53 gene mutations in gastric adenomas. 胃腺瘤中P53基因突变。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02899260

H Tohdo, H Yokozaki, K Haruma, G Kajiyama, E Tahara

引用次数: 63

Correlation of the expression of double-stranded RNA-dependent protein kinase (p68) with differentiation in head and neck squamous cell carcinoma. 双链rna依赖蛋白激酶(p68)表达与头颈部鳞状细胞癌分化的相关性

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02899275

G K Haines, G D Ghadge, S Becker, M Kies, H Pelzer, B Thimmappaya, J A Radosevich

{"title":"Correlation of the expression of double-stranded RNA-dependent protein kinase (p68) with differentiation in head and neck squamous cell carcinoma.","authors":"G K Haines, G D Ghadge, S Becker, M Kies, H Pelzer, B Thimmappaya, J A Radosevich","doi":"10.1007/BF02899275","DOIUrl":"https://doi.org/10.1007/BF02899275","url":null,"abstract":"p68 is an inducible protein kinase which is believed to be an important factor in the regulation of both viral and cellular protein synthesis. We have produced a monoclonal antibody (TJ4C4) which specifically detects p68, and which can be used to detect this antigen in formalin-fixed, paraffin-embedded tissues. Because p68 plays an important role in cellular protein synthesis, we hypothesized that it may correlate with normal and neoplastic cellular differentiation. One hundred and seventy-seven head and neck squamous cell carcinoma specimens, representing 82 patients, were studied. The relative amount, frequency, and distribution of p68 expression were determined by microscopic evaluation of ABC immunoperoxidase-stained specimens. A spectrum of immunoreactivity was detected in 156 of 177 tumors, as well as within the normal squamous epithelium. Normal, actively proliferating cells, such as the basal layer of squamous epithelium, expressed comparatively little p68. Increased p68 expression was noted to parallel the morphologic features of cellular differentiation. In neoplastic tissue, p68 expression also increased with the degree of cellular differentiation. These data demonstrate that the expression of p68 parallels the degree of cellular differentiation in squamous cell carcinoma of the head and neck region, as well as within normal squamous mucosa. Therefore, p68 may provide an objective biologic measure of cellular differentiation which does not depend on morphologic features.","PeriodicalId":23521,"journal":{"name":"Virchows Archiv. B, Cell pathology including molecular pathology","volume":"63 5","pages":"289-95"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02899275","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19092820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Proliferating cell nuclear antigen in normal urothelium and urothelial lesions of the urinary bladder: a quantitative assessment using a true color image analysis system. 增殖细胞核抗原在正常尿路上皮和尿路上皮病变膀胱:定量评估使用真彩色图像分析系统。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02915106

R Knuechel, M Burgau, J Rueschoff, F Hofstaedter

{"title":"Proliferating cell nuclear antigen in normal urothelium and urothelial lesions of the urinary bladder: a quantitative assessment using a true color image analysis system.","authors":"R Knuechel, M Burgau, J Rueschoff, F Hofstaedter","doi":"10.1007/BF02915106","DOIUrl":"https://doi.org/10.1007/BF02915106","url":null,"abstract":"To evaluate proliferating cell nuclear antigen (PCNA) staining for assessing proliferative activity in routine pathology specimens of urinary bladder, the bladder carcinoma cell line J82 and a total of 122 specimens of normal bladder and urothelial lesions were stained with the antibody clone PC10 against proliferating cell nuclear antigen. In in vitro plateau cultures the proportion of PCNA-positive cells exceeded that of Ki-67-positive cells, and only very few cells were negative. In formalin-fixed tissues, the PCNA staining pattern, which should be confined to replicon units in the nucleus, was optimized by 1 h postfixation in an organic solvent (methacarn). Sections showed positive nuclear staining confined to basal and some suprabasal cells in normal urothelium and grade 1 dysplasias, but more generalized nuclear staining in all other neoplastic lesions. In addition, stromal cells adjacent to invasive tumors showed nuclear positivity in some instances. Using quantitative true color image analysis of sections counterstained with hemalum, the degree of brown staining of the PCNA reaction product is contrasted with the blue staining of the nuclear area. With this method low contrast specific staining not appreciated optically can be reliably detected. Image analysis data confirmed observations made on noncounterstained sections and showed significant differences between grade 1 and 2 dysplasias as well as between grade 1 dysplasia and all grades of papillary tumor. Furthermore, a significant difference in PCNA staining indices was found between grade 1 and 3 bladder carcinomas. The results indicate that PCNA staining using the PC10 antibody is not confined to the proliferative fraction of neoplastic urothelium.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":23521,"journal":{"name":"Virchows Archiv. B, Cell pathology including molecular pathology","volume":"64 3","pages":"137-44"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02915106","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18899374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

GM-CSF-induced granulation tissue formation: relationships between macrophage and myofibroblast accumulation. gm - csf诱导的肉芽组织形成:巨噬细胞与肌成纤维细胞积累之间的关系。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02899267

S Vyalov, A Desmoulière, G Gabbiani

{"title":"GM-CSF-induced granulation tissue formation: relationships between macrophage and myofibroblast accumulation.","authors":"S Vyalov, A Desmoulière, G Gabbiani","doi":"10.1007/BF02899267","DOIUrl":"https://doi.org/10.1007/BF02899267","url":null,"abstract":"We have studied the formation of granulation tissue around osmotic minipumps delivering granulocyte macrophage-colony stimulating factor (GM-CSF) chronologically in the rat using electron microscopy and immunohistochemistry at the light and electron microscopic levels, with specific antibodies against alpha-smooth muscle (SM) actin and rat macrophages. At 2 and 3 days after pump implantation, GM-CSF application produced an extensive inflammatory reaction characterized by edema and the accumulation of polymorphonuclear cells and macrophages. Gradually, polymorphonuclear cells decreased in number and macrophages became arranged in large clusters. The expression of alpha-SM actin in fibroblastic cells of the granulation tissue started from the 4th day after pump implantation and progressed up to the 7th day. Double immunofluorescence staining showed macrophage clusters in relation to alpha-SM actin-rich fibroblastic cells. Electron microscopic examination confirmed that the fibroblasts containing alpha-SM actin-positive stress fibers were found initially in close proximity to clustered macrophages. The delivery of platelet-derived growth factor (PDGF) and tumor necrosis factor-alpha (TNF-alpha) by the osmotic minipump induced an accumulation of macrophages, but in a much smaller number compared with those seen after GM-CSF application; these macrophages were never assembled in clusters and, furthermore, TNF-alpha and PDGF did not stimulate alpha-SM actin expression in fibroblastic cells. Our results suggest that after GM-CSF administration, the cluster-like accumulation of macrophages plays an important role in stimulating alpha-SM actin expression in myofibroblasts. Our results may be relevant to the understanding of the processes leading to granulation tissue formation in this and other experimental models.","PeriodicalId":23521,"journal":{"name":"Virchows Archiv. B, Cell pathology including molecular pathology","volume":"63 4","pages":"231-9"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02899267","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19091906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 110

An immunohistochemical study of human pituicytes demonstrating frequent expression of MHC class II antigens and macrophage markers. 人垂体细胞的免疫组织化学研究显示MHC II类抗原和巨噬细胞标记物的频繁表达。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02915138

A Sasaki, A Ogawa, J Hirato, Y Nakazato

{"title":"An immunohistochemical study of human pituicytes demonstrating frequent expression of MHC class II antigens and macrophage markers.","authors":"A Sasaki, A Ogawa, J Hirato, Y Nakazato","doi":"10.1007/BF02915138","DOIUrl":"https://doi.org/10.1007/BF02915138","url":null,"abstract":"Using a panel of antibodies (Abs) and a lectin, normal human adult pituicytes were studied in neurohypophyses obtained from 29 patients at autopsy. The pituicytes reacted frequently with Abs against major histocompatibility complex (MHC) class II antigens (Ags), macrophage markers (KP.1, PG.M1, LN-5), an anti-vimentin Ab and a biotinylated lectin Ricinus communis agglutinin (RCA-1). The number of pituicytes immunostained for these reagents varied, with the notable exception of vimentin. MHC class II Abs (LN-3, CR3.43)-positive pituicytes were numerous in approximately half. Microscopically, MHC class II Ag was found in pituicytes of various shapes, and were identified in macrophage-typed pituicytes by electron microscopic immunohistochemistry. Glial fibrillary acidic protein was found in only a small number of pituicytes and was absent in cells labeled with MHC class II Abs or macrophage markers. The results indicate that the immunophenotype of human pituicytes is distinct from other glial cells of the central nervous system, with a considerable number of cells expressing MHC class II Ags and macrophage markers.","PeriodicalId":23521,"journal":{"name":"Virchows Archiv. B, Cell pathology including molecular pathology","volume":"64 6","pages":"379-86"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02915138","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19139651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Identification of p53 gene mutations in gastrointestinal and pancreatic carcinoids by nonradioisotopic SSCA. 非放射性同位素SSCA鉴定胃肠道和胰腺癌中p53基因突变。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02915125

D R Lohmann, A Funk, H P Niedermeyer, S Häupel, H Höfler

引用次数: 69

Characterization of the inflammatory cell populations in normal colon and colonic carcinomas. 正常结肠癌和结肠癌中炎性细胞群的特征。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02915115

B F Banner, L Savas, S Baker, B A Woda

{"title":"Characterization of the inflammatory cell populations in normal colon and colonic carcinomas.","authors":"B F Banner, L Savas, S Baker, B A Woda","doi":"10.1007/BF02915115","DOIUrl":"https://doi.org/10.1007/BF02915115","url":null,"abstract":"Unlabelled: Little is known about the nature of the mucosa-associated immune system within the normal colon, or about the immune response to colon carcinoma. In this study inflammatory cells (ICs) in 14 normal colons and 14 carcinomas were characterized. Overall inflammation, lymphocytes, plasma cells, neutrophils, and eosinophils were graded in routine H & E sections. Frozen sections were stained by an immunoperoxidase technique using antibodies to the T cell associated antigens CD2, CD7, CD4, CD8, and T cell receptors alpha beta and gamma delta. B cells were identified with CD20, macrophages with CD68, and Class II antigen with anti-HLA DR. Each cell type was semiquantitatively graded in 10 high power fields (HPFs) in the lumenal half (LH) or basal half (BH) of the normal mucosae, and in epithelium or stroma of the carcinomas. In normal colons, ICs were more frequent in LH than in BH. Plasma cells, lymphocytes and monocytes predominated. Subtyping of lymphocytes showed that CD4+ TCR alpha beta + T lymphocytes were most numerous in the lamina propria. Lymphocytes within the epithelium were CD8+ T cells. Around carcinomas the overall grade of ICs was 1+ in the majority of cases. Plasma cells, CD4+ and CD8+ cells with the TCR alpha beta receptor, and macrophages were most frequent. Lymphoid aggregates of both T and B cells were frequent.Conclusions: 1. Normal colon contains a diffuse luminally oriented population of TCR alpha beta+ CD4+ T cells, plasma cells, macrophages and class II antigen-expressing cells in the lamina propria.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":23521,"journal":{"name":"Virchows Archiv. B, Cell pathology including molecular pathology","volume":"64 4","pages":"213-20"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02915115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19273959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Differential expression of alpha 1-antichymotrypsin in the aged human brain. α 1-抗凝乳胰蛋白酶在老年人脑中的差异表达。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02915116

K Ishiguro, M Shoji, H Yamaguchi, E Matsubara, M Ikeda, T Kawarabayashi, Y Harigaya, K Okamoto, S Hirai

{"title":"Differential expression of alpha 1-antichymotrypsin in the aged human brain.","authors":"K Ishiguro, M Shoji, H Yamaguchi, E Matsubara, M Ikeda, T Kawarabayashi, Y Harigaya, K Okamoto, S Hirai","doi":"10.1007/BF02915116","DOIUrl":"https://doi.org/10.1007/BF02915116","url":null,"abstract":"The localization of alpha 1-antichymotrypsin (ACT) mRNA and ACT immunoreactivity (ACT-IR) were examined in 12 brains obtained at post-mortem from elderly patients, four of whom had Alzheimer's disease. A biotinylated oligonucleotide probe was used for in situ hybridization histochemistry and the relationship between the expression of both ACT mRNA and ACT-IR and the extent of beta protein or tau deposition were investigated. The extent of beta-plaques, tau-tangles, and ACT-IR were rated from (-) to (++). In brains without plaques and tangles, there were no detectable ACT mRNA signals in the gray matter, and those in the white matter were weak; in these brains, ACT-IR was generally weak. The brains with beta-plaques but no tangles showed weak ACT mRNA expression in astrocytes of both the gray and white matter; they also showed weak ACT-IR in the astrocytes. In the brains from patients with Alzheimer's disease with both plaques and tangles, ACT mRNA was expressed intensely in a majority of the astrocytes in the white and gray matter. Some senile plaques-associated astrocytes expressed ACT mRNA and ACT-IR was strong in the white matter astrocytes. ACT-IR and ACT mRNA expression in astrocytes was correlated with the extent of beta and tau depositions.","PeriodicalId":23521,"journal":{"name":"Virchows Archiv. B, Cell pathology including molecular pathology","volume":"64 4","pages":"221-7"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02915116","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19273960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Protective effect of silymarin on rat liver injury induced by ischemia. 水飞蓟素对大鼠缺血肝损伤的保护作用。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02915120

C G Wu, R A Chamuleau, K S Bosch, W M Frederiks

{"title":"Protective effect of silymarin on rat liver injury induced by ischemia.","authors":"C G Wu, R A Chamuleau, K S Bosch, W M Frederiks","doi":"10.1007/BF02915120","DOIUrl":"https://doi.org/10.1007/BF02915120","url":null,"abstract":"The effect of silymarin on liver cell damage induced by ischemia was studied in rats fasted for 24 h. In the first series of experiments in vitro ischemia was induced by storing tissue blocks in closed vials at 37 degrees C for 15, 30, 45 and 60 min. Cell injury was detected by the cytophotometrical measurement of glycogen phosphorylase activity in unfixed cryostat sections demonstrated by a modified histochemical procedure. In the second series of experiments in vivo ischemia was provoked by clamping the afferent vessels to the median and left lateral lobes of the liver for 60 min, followed by removal of the clamp and reperfusion. The extent of cell damage was determined by measuring the ALAT and ASAT activities in serum at 1, 3, 6 and 24 h after ischemia and by quantifying the extent of necrosis in the liver after 24 h reperfusion by measuring the unstained areas in cryostat sections incubated for lactate dehydrogenase activity. Silymarin (100 mg/kg b.w.) was administered intravenously at 5 min before both the induction of ischemia and the restoration of blood flow (in vivo ischemia) and at 1 h and at 5 min before sacrifice (in vitro ischemia). Controls received an equal amount of saline. The serum amino-transferase activities after 24 h reperfusion were significantly reduced in the silymarin-treated group (n = 10); ALAT 293 +/- 193 U/L, ASAT 343 +/- 229 U/L compared with the control group (n = 7): ALAT 1238 +/- 743 U/L, ASAT 948 +/- 541 U/L (p < 0.03), and the extent of necrosis decreased from 25.6 +/- 16.0% ( n = 7) to 7.8 +/- 8.3% (n = 10) (p < 0.01) after treatment with silymarin.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":23521,"journal":{"name":"Virchows Archiv. B, Cell pathology including molecular pathology","volume":"64 5","pages":"259-63"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02915120","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19273964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 42