Virchows Archiv. B, Cell pathology including molecular pathology最新文献_第2页

Proliferating cell nuclear antigen in normal urothelium and urothelial lesions of the urinary bladder: a quantitative assessment using a true color image analysis system. 增殖细胞核抗原在正常尿路上皮和尿路上皮病变膀胱:定量评估使用真彩色图像分析系统。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02915106

R Knuechel, M Burgau, J Rueschoff, F Hofstaedter

{"title":"Proliferating cell nuclear antigen in normal urothelium and urothelial lesions of the urinary bladder: a quantitative assessment using a true color image analysis system.","authors":"R Knuechel, M Burgau, J Rueschoff, F Hofstaedter","doi":"10.1007/BF02915106","DOIUrl":"https://doi.org/10.1007/BF02915106","url":null,"abstract":"To evaluate proliferating cell nuclear antigen (PCNA) staining for assessing proliferative activity in routine pathology specimens of urinary bladder, the bladder carcinoma cell line J82 and a total of 122 specimens of normal bladder and urothelial lesions were stained with the antibody clone PC10 against proliferating cell nuclear antigen. In in vitro plateau cultures the proportion of PCNA-positive cells exceeded that of Ki-67-positive cells, and only very few cells were negative. In formalin-fixed tissues, the PCNA staining pattern, which should be confined to replicon units in the nucleus, was optimized by 1 h postfixation in an organic solvent (methacarn). Sections showed positive nuclear staining confined to basal and some suprabasal cells in normal urothelium and grade 1 dysplasias, but more generalized nuclear staining in all other neoplastic lesions. In addition, stromal cells adjacent to invasive tumors showed nuclear positivity in some instances. Using quantitative true color image analysis of sections counterstained with hemalum, the degree of brown staining of the PCNA reaction product is contrasted with the blue staining of the nuclear area. With this method low contrast specific staining not appreciated optically can be reliably detected. Image analysis data confirmed observations made on noncounterstained sections and showed significant differences between grade 1 and 2 dysplasias as well as between grade 1 dysplasia and all grades of papillary tumor. Furthermore, a significant difference in PCNA staining indices was found between grade 1 and 3 bladder carcinomas. The results indicate that PCNA staining using the PC10 antibody is not confined to the proliferative fraction of neoplastic urothelium.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":23521,"journal":{"name":"Virchows Archiv. B, Cell pathology including molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02915106","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18899374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

GM-CSF-induced granulation tissue formation: relationships between macrophage and myofibroblast accumulation. gm - csf诱导的肉芽组织形成:巨噬细胞与肌成纤维细胞积累之间的关系。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02899267

S Vyalov, A Desmoulière, G Gabbiani

{"title":"GM-CSF-induced granulation tissue formation: relationships between macrophage and myofibroblast accumulation.","authors":"S Vyalov, A Desmoulière, G Gabbiani","doi":"10.1007/BF02899267","DOIUrl":"https://doi.org/10.1007/BF02899267","url":null,"abstract":"We have studied the formation of granulation tissue around osmotic minipumps delivering granulocyte macrophage-colony stimulating factor (GM-CSF) chronologically in the rat using electron microscopy and immunohistochemistry at the light and electron microscopic levels, with specific antibodies against alpha-smooth muscle (SM) actin and rat macrophages. At 2 and 3 days after pump implantation, GM-CSF application produced an extensive inflammatory reaction characterized by edema and the accumulation of polymorphonuclear cells and macrophages. Gradually, polymorphonuclear cells decreased in number and macrophages became arranged in large clusters. The expression of alpha-SM actin in fibroblastic cells of the granulation tissue started from the 4th day after pump implantation and progressed up to the 7th day. Double immunofluorescence staining showed macrophage clusters in relation to alpha-SM actin-rich fibroblastic cells. Electron microscopic examination confirmed that the fibroblasts containing alpha-SM actin-positive stress fibers were found initially in close proximity to clustered macrophages. The delivery of platelet-derived growth factor (PDGF) and tumor necrosis factor-alpha (TNF-alpha) by the osmotic minipump induced an accumulation of macrophages, but in a much smaller number compared with those seen after GM-CSF application; these macrophages were never assembled in clusters and, furthermore, TNF-alpha and PDGF did not stimulate alpha-SM actin expression in fibroblastic cells. Our results suggest that after GM-CSF administration, the cluster-like accumulation of macrophages plays an important role in stimulating alpha-SM actin expression in myofibroblasts. Our results may be relevant to the understanding of the processes leading to granulation tissue formation in this and other experimental models.","PeriodicalId":23521,"journal":{"name":"Virchows Archiv. B, Cell pathology including molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02899267","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19091906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 110

An immunohistochemical study of human pituicytes demonstrating frequent expression of MHC class II antigens and macrophage markers. 人垂体细胞的免疫组织化学研究显示MHC II类抗原和巨噬细胞标记物的频繁表达。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02915138

A Sasaki, A Ogawa, J Hirato, Y Nakazato

{"title":"An immunohistochemical study of human pituicytes demonstrating frequent expression of MHC class II antigens and macrophage markers.","authors":"A Sasaki, A Ogawa, J Hirato, Y Nakazato","doi":"10.1007/BF02915138","DOIUrl":"https://doi.org/10.1007/BF02915138","url":null,"abstract":"Using a panel of antibodies (Abs) and a lectin, normal human adult pituicytes were studied in neurohypophyses obtained from 29 patients at autopsy. The pituicytes reacted frequently with Abs against major histocompatibility complex (MHC) class II antigens (Ags), macrophage markers (KP.1, PG.M1, LN-5), an anti-vimentin Ab and a biotinylated lectin Ricinus communis agglutinin (RCA-1). The number of pituicytes immunostained for these reagents varied, with the notable exception of vimentin. MHC class II Abs (LN-3, CR3.43)-positive pituicytes were numerous in approximately half. Microscopically, MHC class II Ag was found in pituicytes of various shapes, and were identified in macrophage-typed pituicytes by electron microscopic immunohistochemistry. Glial fibrillary acidic protein was found in only a small number of pituicytes and was absent in cells labeled with MHC class II Abs or macrophage markers. The results indicate that the immunophenotype of human pituicytes is distinct from other glial cells of the central nervous system, with a considerable number of cells expressing MHC class II Ags and macrophage markers.","PeriodicalId":23521,"journal":{"name":"Virchows Archiv. B, Cell pathology including molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02915138","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19139651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Identification of p53 gene mutations in gastrointestinal and pancreatic carcinoids by nonradioisotopic SSCA. 非放射性同位素SSCA鉴定胃肠道和胰腺癌中p53基因突变。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02915125

D R Lohmann, A Funk, H P Niedermeyer, S Häupel, H Höfler

引用次数: 69

Tenascin in inflammatory conditions and neoplasms of the urinary bladder. 膀胱炎症和肿瘤中的腱素。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02899274

O Tiitta, T Wahlström, I Virtanen, V E Gould

{"title":"Tenascin in inflammatory conditions and neoplasms of the urinary bladder.","authors":"O Tiitta, T Wahlström, I Virtanen, V E Gould","doi":"10.1007/BF02899274","DOIUrl":"https://doi.org/10.1007/BF02899274","url":null,"abstract":"Tenascin (Tn) is an extracellular matrix (ECM) glycoprotein strongly and widely expressed during embryogenesis. Tn is decreased in normal adult tissues but is reexpressed in numerous inflammatory, reparative and neoplastic processes. We immunostained samples of fetal and normal adult bladders and samples of bladder tissue from patients with chronic cystitis, detrusor hypertrophy, malakoplakia and transitional cell carcinomas (TCC) of all grades, with a monoclonal antibody (mAb) to Tn 143DB7. Sections of flat in situ carcinomas were also studied. In fetal bladders, strong and ragged Tn reactions were noted at the epithelial-stromal interface; in normal adult bladders, the reaction was delicate and less extensive. In chronic cystitis, Tn reactivity was enhanced particularly around prominent capillary blood vessels. In flat in situ carcinomas, Tn staining was stronger and more extensive than in normal mucosa but was often less extensive than in some examples of cystitis. In TCC I and II, Tn immunoreactivity was strong and predominated in the pericapillary stroma of the papillae; in infiltrating TCC II, comparatively limited Tn staining was noted. In deeply infiltrating grade III TCC with abundant stroma, Tn reaction was invariably strong and extensive, particularly around advancing tumor nests. The strongest Tn reactions were noted in invasive, high-grade TCC with abundant stroma. We conclude that in inflammatory-reactive processes, and in in situ carcinomas as well as in TCC, the extent and intensity of the Tn reaction correlates with the severity of the inflammatory infiltrate and with the extent of the stromal remodelling.","PeriodicalId":23521,"journal":{"name":"Virchows Archiv. B, Cell pathology including molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02899274","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18688735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 36

p53 gene mutations in gastric adenomas. 胃腺瘤中P53基因突变。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02899260

H Tohdo, H Yokozaki, K Haruma, G Kajiyama, E Tahara

引用次数: 63

Correlation of the expression of double-stranded RNA-dependent protein kinase (p68) with differentiation in head and neck squamous cell carcinoma. 双链rna依赖蛋白激酶(p68)表达与头颈部鳞状细胞癌分化的相关性

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02899275

G K Haines, G D Ghadge, S Becker, M Kies, H Pelzer, B Thimmappaya, J A Radosevich

{"title":"Correlation of the expression of double-stranded RNA-dependent protein kinase (p68) with differentiation in head and neck squamous cell carcinoma.","authors":"G K Haines, G D Ghadge, S Becker, M Kies, H Pelzer, B Thimmappaya, J A Radosevich","doi":"10.1007/BF02899275","DOIUrl":"https://doi.org/10.1007/BF02899275","url":null,"abstract":"p68 is an inducible protein kinase which is believed to be an important factor in the regulation of both viral and cellular protein synthesis. We have produced a monoclonal antibody (TJ4C4) which specifically detects p68, and which can be used to detect this antigen in formalin-fixed, paraffin-embedded tissues. Because p68 plays an important role in cellular protein synthesis, we hypothesized that it may correlate with normal and neoplastic cellular differentiation. One hundred and seventy-seven head and neck squamous cell carcinoma specimens, representing 82 patients, were studied. The relative amount, frequency, and distribution of p68 expression were determined by microscopic evaluation of ABC immunoperoxidase-stained specimens. A spectrum of immunoreactivity was detected in 156 of 177 tumors, as well as within the normal squamous epithelium. Normal, actively proliferating cells, such as the basal layer of squamous epithelium, expressed comparatively little p68. Increased p68 expression was noted to parallel the morphologic features of cellular differentiation. In neoplastic tissue, p68 expression also increased with the degree of cellular differentiation. These data demonstrate that the expression of p68 parallels the degree of cellular differentiation in squamous cell carcinoma of the head and neck region, as well as within normal squamous mucosa. Therefore, p68 may provide an objective biologic measure of cellular differentiation which does not depend on morphologic features.","PeriodicalId":23521,"journal":{"name":"Virchows Archiv. B, Cell pathology including molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02899275","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19092820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

The impact of aging on enzyme proteins of rat liver peroxisomes: quantitative analysis by immunoblotting and immunoelectron microscopy. 衰老对大鼠肝脏过氧化物酶体酶蛋白的影响:免疫印迹和免疫电镜定量分析。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02899254

K Beier, A Völkl, H D Fahimi

{"title":"The impact of aging on enzyme proteins of rat liver peroxisomes: quantitative analysis by immunoblotting and immunoelectron microscopy.","authors":"K Beier, A Völkl, H D Fahimi","doi":"10.1007/BF02899254","DOIUrl":"https://doi.org/10.1007/BF02899254","url":null,"abstract":"The alterations of hepatic peroxisomes and their enzymes during aging were investigated in male rats. Peroxisomes in the livers of young (2 months) and old (39 months) male Wistar rats were analyzed by morphometry and quantitative immunocytochemistry, as well as by immunoblotting of highly purified peroxisomal fractions. Immunoblots showed that catalase and acyl-CoA oxidase were decreased in peroxisomes of old animals but the trifunctional enzyme, thiolase, and urate oxidase were increased. The morphometrical analysis revealed a heterogeneous distribution of peroxisomes in the liver lobule of the old animals, with a significant elevation of peroxisomal volume density in pericentral over periportal hepatocytes, in contrast to the uniform pattern in the young rats. Furthermore, age-related lobular gradients were also observed by quantitative immunocytochemistry in the peroxisomal concentrations of trifunctional enzyme (central > portal) and, inversely, for catalase (portal > central). Whereas acyl-CoA oxidase was diminished across the liver lobule, the enzyme 3-ketoacyl-CoA thiolase was elevated. These observations show that peroxisomes are significantly altered in aged animals and suggest that these alterations may contribute to the disturbance of lipid metabolism in aged animals. Moreover, the diminution in catalase and the elevation of urate oxidase could contribute to the oxidative stress which is considered to be of fundamental importance in the aging process.","PeriodicalId":23521,"journal":{"name":"Virchows Archiv. B, Cell pathology including molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02899254","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19089200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 52

Characterization of four new gastric cancer cell lines. 四种新型胃癌细胞系的鉴定。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02899281

H P Vollmers, K Stulle, J Dämmrich, M Pfaff, T Papadopoulos, C Betz, K Saal, H K Müller-Hermelink

引用次数: 32

Tumor necrosis factor alpha (TNF alpha) and transforming growth factor beta 1 (TGF beta 1) stimulate fibronectin synthesis and the transdifferentiation of fat-storing cells in the rat liver into myofibroblasts. 肿瘤坏死因子α (TNF α)和转化生长因子β 1 (TGF β 1)刺激大鼠肝脏中脂肪储存细胞的纤维连接蛋白合成和向肌成纤维细胞的转分化。

Virchows Archiv. B, Cell pathology including molecular pathology Pub Date : 1993-01-01 DOI: 10.1007/BF02899251

M G Bachem, K M Sell, R Melchior, J Kropf, T Eller, A M Gressner

{"title":"Tumor necrosis factor alpha (TNF alpha) and transforming growth factor beta 1 (TGF beta 1) stimulate fibronectin synthesis and the transdifferentiation of fat-storing cells in the rat liver into myofibroblasts.","authors":"M G Bachem, K M Sell, R Melchior, J Kropf, T Eller, A M Gressner","doi":"10.1007/BF02899251","DOIUrl":"https://doi.org/10.1007/BF02899251","url":null,"abstract":"Transforming growth factor-beta (TGF beta 1) and tumor necrosis factor alpha (TNF alpha) stimulate the transdifferentiation of fat-storing cells (FSC) in the rat liver into highly active and \"synthetic\" myofibroblast-like cells (MFBIC). This activation has been documented by differential-interference contrast and light microscopy using morphologic criteria (a reduction in the number and size of fat droplets, cell flattening and the development of long cytoplasmic extensions), by the loss of retinyl-palmitate (measured by HPLC) and by the enhanced expression of iso-alpha smooth muscle actin (demonstrated by immunofluorescence microscopy). Furthermore, while cell growth measured by the cell count and DNA content is slightly inhibited by TGF beta 1 (0.81 of the control), the combination of TGF beta 1 with TNF alpha stimulates cell proliferation to 1.44 times of the control. In addition the combination of TGF beta and TNF alpha potentiated the stimulatory effect on fibronectin synthesis (TGF beta alone: 1.4 times control; TNF alpha alone: 2.2 times control; TGF beta plus TNF alpha: 4.7 times control). The total protein synthesis was not altered by TGF beta or TNF alpha. In summary the results obtained identify TGF beta and TNF alpha as mediators stimulating key events in liver fibrogenesis (i.e. FSC proliferation, FSC transdifferentiation into MFBIC, and fibronectin synthesis).","PeriodicalId":23521,"journal":{"name":"Virchows Archiv. B, Cell pathology including molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02899251","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19088018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 122