Proliferating cell nuclear antigen in normal urothelium and urothelial lesions of the urinary bladder: a quantitative assessment using a true color image analysis system.

R Knuechel, M Burgau, J Rueschoff, F Hofstaedter
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引用次数: 5

Abstract

To evaluate proliferating cell nuclear antigen (PCNA) staining for assessing proliferative activity in routine pathology specimens of urinary bladder, the bladder carcinoma cell line J82 and a total of 122 specimens of normal bladder and urothelial lesions were stained with the antibody clone PC10 against proliferating cell nuclear antigen. In in vitro plateau cultures the proportion of PCNA-positive cells exceeded that of Ki-67-positive cells, and only very few cells were negative. In formalin-fixed tissues, the PCNA staining pattern, which should be confined to replicon units in the nucleus, was optimized by 1 h postfixation in an organic solvent (methacarn). Sections showed positive nuclear staining confined to basal and some suprabasal cells in normal urothelium and grade 1 dysplasias, but more generalized nuclear staining in all other neoplastic lesions. In addition, stromal cells adjacent to invasive tumors showed nuclear positivity in some instances. Using quantitative true color image analysis of sections counterstained with hemalum, the degree of brown staining of the PCNA reaction product is contrasted with the blue staining of the nuclear area. With this method low contrast specific staining not appreciated optically can be reliably detected. Image analysis data confirmed observations made on noncounterstained sections and showed significant differences between grade 1 and 2 dysplasias as well as between grade 1 dysplasia and all grades of papillary tumor. Furthermore, a significant difference in PCNA staining indices was found between grade 1 and 3 bladder carcinomas. The results indicate that PCNA staining using the PC10 antibody is not confined to the proliferative fraction of neoplastic urothelium.(ABSTRACT TRUNCATED AT 250 WORDS)

增殖细胞核抗原在正常尿路上皮和尿路上皮病变膀胱:定量评估使用真彩色图像分析系统。
为了评价增殖细胞核抗原(PCNA)染色在膀胱常规病理标本中的增殖活性,我们用增殖细胞核抗原克隆PC10对膀胱癌细胞株J82和122例正常膀胱和尿路上皮病变标本进行染色。在体外平台培养中,pcna阳性细胞的比例超过了ki -67阳性细胞,只有极少数细胞呈阴性。在福尔马林固定的组织中,PCNA的染色模式应该局限于细胞核内的复制子单元,通过在有机溶剂(甲基萘)中固定1小时来优化。切片显示阳性核染色局限于正常尿路上皮和1级发育不良的基底细胞和一些基底上细胞,但在所有其他肿瘤病变中均有更广泛的核染色。此外,侵袭性肿瘤附近的间质细胞在某些情况下显示核阳性。用定量的真彩色图像分析与血红素反染的切片,将PCNA反应产物的棕色染色程度与核区蓝色染色程度进行对比。用这种方法可以可靠地检测到不受光学欣赏的低对比度特异性染色。图像分析数据证实了未反染色切片的观察结果,并显示1级和2级发育不良之间以及1级发育不良与所有级别乳头状瘤之间存在显著差异。此外,PCNA染色指数在1级和3级膀胱癌之间存在显著差异。结果表明,使用PC10抗体的PCNA染色并不局限于肿瘤尿路上皮的增殖部分。(摘要删节250字)
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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