Tran Thi Van Anh, Ha Thanh Hoa, Pham Quoc Tuan, Ngo Thi Sau, Doan Thi Mai Huong, Nguyen Van Thang, Tran Van Thao, Nguyen Thi Thanh Hai, Dao Viet Hung
{"title":"Evaluation of Protein Kinase C Inhibition of some Benzofuro[3,2-d]pyrimidine Derivatives","authors":"Tran Thi Van Anh, Ha Thanh Hoa, Pham Quoc Tuan, Ngo Thi Sau, Doan Thi Mai Huong, Nguyen Van Thang, Tran Van Thao, Nguyen Thi Thanh Hai, Dao Viet Hung","doi":"10.25073/2588-1132/vnumps.4493","DOIUrl":"https://doi.org/10.25073/2588-1132/vnumps.4493","url":null,"abstract":"Abstract: Treatment of fungal infections is very difficult because of the limited therapeutic arsenal and the emergence of resistance to the two major antifungal classes: azoles and echinocandins. Consequently, finding new targets and new therapeutic strategies is a priority. The protein kinase Pkc1 of Candida albicans (CaPkc1), one of the key proteins involved in MAPK pathways, is described as a regulator of cell wall integrity during growth, morphogenesis, and response to cell wall stress. Therefore, the discovery of CaPkc1 inhibitory molecules can open up new and promising prospects in the development of new antifungal drugs that inhibit CaPkc1. In this study, we evaluated the CaPkc1 inhibitory activity of three benzofuro[3,2-d]pyrimidine derivatives at three different concentrations of 50 µM, 100 µM, and 150 µM. A concentration of 100 μM was optimal for CaPkc1 inhibitory activity with all three benzofuro[3,2-d]pyrimidine derivatives. \u0000Keywords: Benzofuro[3,2-d]pyrimidine, CaPkc1, Candida albicans, antifungal activity. \u0000 \u0000 ","PeriodicalId":23520,"journal":{"name":"VNU Journal of Science: Medical and Pharmaceutical Sciences","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80326577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dinh Dieu Hong, Hoang Trung Kien, Nguyen Thi Minh Huyen, Nguyen Yen Nhi, Dinh Thai Bao
{"title":"Clinical Signs of Temporomandibular Joint Dysfunction in Students of a University in Hanoi","authors":"Dinh Dieu Hong, Hoang Trung Kien, Nguyen Thi Minh Huyen, Nguyen Yen Nhi, Dinh Thai Bao","doi":"10.25073/2588-1132/vnumps.4451","DOIUrl":"https://doi.org/10.25073/2588-1132/vnumps.4451","url":null,"abstract":"Abstract: Temporomandibular joint dysfunction (TMD) is a common disease, especially increasing in young people, and affects the quality of life. Clinical signs of TMDs are various and complex. Diagnosis of symptoms and prognosis is essential. Therefore, we conducted a cross-sectional descriptive study on 61 patients aged 19-23 who participated voluntarily from February to June 2022 at the Odonto-Stomatology clinic at the University of Medicine and Pharmacy, Vietnam National University. The results showed that the proportion of women with symptoms was higher than that of men. For the clinical symptoms, percentages of the limited range of mandibular movements and mouth gap deviation were quite high (77.0% and 68.9%, respectively). Joint sounds and arthralgia took 31.1% and 24.6%, while myalgia accounted for only 11.5%. In the joint, the pain was detected mainly on the outer surface of the joint (24.6%). In both genders, the masseter muscle was the most painful muscle group. Clinical symptoms are very important in diagnosis, treatment, and prognosis. \u0000Keywords: Clinical signs, temporomandibular joint dysfunction, students. \u0000 \u0000 ","PeriodicalId":23520,"journal":{"name":"VNU Journal of Science: Medical and Pharmaceutical Sciences","volume":"10 10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84009540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nguyen Nhu Dua, Doan Thi Hong Hoa, Nguyen Tuan Son, Nguyen Le Hoa
{"title":"The Clinical Characteristics of Acute Otitis Media at Several Hospitals in Hanoi from 2019 to 2020","authors":"Nguyen Nhu Dua, Doan Thi Hong Hoa, Nguyen Tuan Son, Nguyen Le Hoa","doi":"10.25073/2588-1132/vnumps.4373","DOIUrl":"https://doi.org/10.25073/2588-1132/vnumps.4373","url":null,"abstract":"Acute otitis media (AOM) is an infection and accumulation of fluid in the middle ear. The disease usually begins suddenly in a short duration and manifests with the symptoms of ear pain, tinnitus, ear discharge, or hearing loss. Clinical characteristics of acute otitis media in children at several hospitals in Hanoi were described from 2019 to 2020. A cross-sectional descriptive study included 40 patients under 16 years old, diagnosed with AOM, and treated at the Department of Otolaryngology at several hospitals in Hanoi from September 2019 to April 2020. The results indicated that the clinical characteristics of AOM included mild fever (37.5%), moderate fever (20%), high fever (22.5%), unilateral otalgia (57.5%), bilateral otalgia (7.5%), no ear discharge (77.5%), and ear discharge (22.5%). In addition, symptoms accompanied by cough, excessive crying, and digestive disorders accounted for 82.5%, 65%, and 30%, respectively. The main symptoms of AOM are fever and otalgia, accompanied by some co-symptoms such as cough, excessive crying, and digestive disorders. \u0000 ","PeriodicalId":23520,"journal":{"name":"VNU Journal of Science: Medical and Pharmaceutical Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86139836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Le Lan Phuong, Tran Le Huyen Linh, Le Thi Thanh Nhan, Bui Phuong Thao, Tran Thi Huyen Trang, Nguyen Van Hung, Nguyen Thi Van Anh, Trinh Hong Thai
{"title":"The Large-scale Deletions and Copy Numbers of Mitochondrial DNA in Gout Patients Treated with Allopurinol","authors":"Le Lan Phuong, Tran Le Huyen Linh, Le Thi Thanh Nhan, Bui Phuong Thao, Tran Thi Huyen Trang, Nguyen Van Hung, Nguyen Thi Van Anh, Trinh Hong Thai","doi":"10.25073/2588-1132/vnumps.4397","DOIUrl":"https://doi.org/10.25073/2588-1132/vnumps.4397","url":null,"abstract":"Allopurinol is now the first-line treatment for managing gout and its increasing usage has brought more gout patients at risk of developing allopurinol-induced hypersensitive reactions. Up to now, our attempts to identify the biomarkers for hypersensitivity to allopurinol are still finite. Besides, the association between drug-induced hypersensitive reactions and some mitochondrial DNA (mtDNA) alterations has been proved but remains unclear in gout patients. In this study, 41 blood samples of gout patients prescribed with allopurinol including 22 hypersensitive and 29 tolerant ones were used to analyze their mtDNA large-scale deletions through PCR and determine the mtDNA deletion level and the copy number by quantitative PCR. The results indicated that the mtDNA large-scale deletions and mtDNA copy number of the hypersensitive group were lower than that of the tolerant one (p < 0.05). However, no association was found between the mtDNA deletion level and the appearance of hypersensitive reactions in gout patients. In addition, the study showed that the mtDNA deletion level and copy number were negatively correlated with the copy numbers of mitochondrial DNA in gout patients (R = -0.3495; p = 0.0119) and allopurinol hypersensitive group (R = -0.6744; p = 0.0005). Thus, a decrease in mtDNA copy number might serve as a potential biomarker for further investigation to assess the risk of hypersensitive reactions to allopurinol in gout patients. \u0000 ","PeriodicalId":23520,"journal":{"name":"VNU Journal of Science: Medical and Pharmaceutical Sciences","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86351065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Role of Ultrasound in the Diagnosis of Gallstones and Assessment of Gallbladder Function","authors":"Doan Van Ngoc, Hoang Dinh Au","doi":"10.25073/2588-1132/vnumps.4357","DOIUrl":"https://doi.org/10.25073/2588-1132/vnumps.4357","url":null,"abstract":"A study of 46 patients diagnosed with gallstones by ultrasound, with indications for assessment of gallbladder function to consider percutaneous gallstone lithotripsy at several hospitals in Hanoi from August 2019 to March 2020. Result: mean age 41.76 ± 16.11, common age 30-50. Gallstones are more common in women than in men, with a male/female ratio of 1:1.1. Average BMI accounted for 76.9%; 45.7% of cases had one stone; gravel with size >10 mm accounted for the majority of 76.1%; 89% of stones were mobile, 95.7% of stones were hyperechoic with shadow. Gallbladder contractility index ≥ 40% accounted for 95.7%. The average gallbladder contractility index was 64.2 ± 16.1%, this index in men was 64.6 ± 15.5%, and in women, it was 63.7 ± 16.9%. 95.7% of cases still had gallbladder contractile function. Conclusion: Ultrasound plays an important role and has many advantages in diagnosing gallstones and evaluating gallbladder function before percutaneous gallstone lithotripsy. \u0000 ","PeriodicalId":23520,"journal":{"name":"VNU Journal of Science: Medical and Pharmaceutical Sciences","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90044092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Anti-peptic Ulcer of the N-hexane Fraction from Sanchezia nobilis Hook.F. Leaves and its Isolated Compounds","authors":"Bui Thi Xuan, Tran Minh Ngoc, Tran Thi Thanh Hoa","doi":"10.25073/2588-1132/vnumps.4424","DOIUrl":"https://doi.org/10.25073/2588-1132/vnumps.4424","url":null,"abstract":"The n-hexane fraction of the Sanchezia nobilis Hook.f. leaves was evaluated for its effect on the gastric ulcer with pyloric ligation (Shay) rat model, analgesia effect on hot plate model and pain threshold meter, and inhibitory effect on Helicobacter pylori (H.P). The data showed a significant reduction in ulcer score, gastric volume, and total acidity and tended to reduce free acidity and increase gastric pH. In addition, the n-hexane fraction had a central analgesic effect and H.P inhibition. Chemical investigation of the n-hexane fraction resulted in the isolation of the four compounds (1 – 4). By using NMR and ESI-MS spectra, these compounds were identified chemical structures (3β, 5α, 22E)-stigmasta-7,22-dien-3-ol (1), coccinic acid (2), 7-hydroxy-6-methoxy coumarin (3), and betulinic acid (4). All compounds were reported for the first time from the Sanchezia genus. \u0000 \u0000 \u0000 ","PeriodicalId":23520,"journal":{"name":"VNU Journal of Science: Medical and Pharmaceutical Sciences","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75974142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nguyen Thi Hai Yen, Nguyen Thi Thanh Thuy, Nguyen Thi Thanh Hai, Nguyen Cong Bang
{"title":"An Initiative Research of the Physicochemical Properties and Fatty Acid Composition of the Crescentia Cujete L. Seed Oil","authors":"Nguyen Thi Hai Yen, Nguyen Thi Thanh Thuy, Nguyen Thi Thanh Hai, Nguyen Cong Bang","doi":"10.25073/2588-1132/vnumps.4376","DOIUrl":"https://doi.org/10.25073/2588-1132/vnumps.4376","url":null,"abstract":"Objectives: To establish the quality standard of the Crescentia cujete L. seed oil. Material and methods: The Crescentia cujete L. seed oil was extracted by Soxhlet system with n-hexane solvent; the acid, peroxide, and iodine values were determined by the methods described in the Vietnamese pharmacopoeia V; GC/MS method was used to determine the composition and the major components of the seed oil. Results and conclusion: The chemical composition and characteristics of Crescentia cujete L. seed oil have been determined. The result showed that seeds have a high oil content (16.44%). The acid, peroxide, and iodine values were 4.02, 9.6, and 92, respectively. A total of 7 fatty acids were identified in the Crescentia cujete L. seed oil (palmitic acid C16:0 – 19.56%, acid stearic C18:0 – 3.85%, nonadecanoic acid C19:0 – 1.20%, eicosanoic acid C20:0 – 0.54%, octadecadienoic acid C18:2 – 73.75%, palmitoleic acid C16:1 – 0.31%, 11-eicosenoic acid C19:1 – 0.15%). These results will be further used in the development of regulatory documents (draft monograph for the Pharmacopoeia) on the Crescentia cujete L. oil. \u0000 ","PeriodicalId":23520,"journal":{"name":"VNU Journal of Science: Medical and Pharmaceutical Sciences","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88273309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Preparation and Valuation of Capsules Containing Quercetin Phytosome for Hepatoprotective Activity","authors":"Vu Thi Thu Giang, P. Huệ, Nguyen Hong Trang","doi":"10.25073/2588-1132/vnumps.4443","DOIUrl":"https://doi.org/10.25073/2588-1132/vnumps.4443","url":null,"abstract":"Quercetin is a well-known flavonoid with different biological effects such as anti-inflammatory, antioxidant, etc. However, this active ingredient was not applied broadly in clinical treatment due to the limitation of solubility, stability, and low oral bioavailability. To overcome this limitation, phytosome quercetin was prepared to enhance quercetin's physicochemical properties and permeability. In the study, prepared phytosome complexes were applied in capsules and evaluated hepatoprotective activity. The study found the optimal formula of capsules containing quercetin phytosome with the active ingredient content of 50 mg that dissolved over 90% of quercetin after 45 minutes. The results of the evaluation of the hepatoprotective activity of the prepared capsules in mice also initially demonstrated that the quercetin phytosome capsules had superior hepatoprotection compared to those containing free quercetin. \u0000 \u0000 ","PeriodicalId":23520,"journal":{"name":"VNU Journal of Science: Medical and Pharmaceutical Sciences","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80516074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tran Thi Van Anh, Dao Viet Hung, Pham Quoc Tuan, Ha Thanh Hoa, Nguyen Thi Minh Diep, Nguyen Van Thang, Ngo Thi Sau, Trinh Thi Duyen, Nguyen Thi Thanh Hai, N. Van Khanh
{"title":"Preparation of Berberine Chloride Nanoparticles by Ball Milling Method","authors":"Tran Thi Van Anh, Dao Viet Hung, Pham Quoc Tuan, Ha Thanh Hoa, Nguyen Thi Minh Diep, Nguyen Van Thang, Ngo Thi Sau, Trinh Thi Duyen, Nguyen Thi Thanh Hai, N. Van Khanh","doi":"10.25073/2588-1132/vnumps.4437","DOIUrl":"https://doi.org/10.25073/2588-1132/vnumps.4437","url":null,"abstract":"Berberine is a quaternary benzylisoquinoline alkaloid that has been used for the local treatment of gastrointestinal tract diseases. Recently, several pharmacological effects of berberine have been reported, which include anti-hypertension, antitumor, anti-hypercholesterolemia, and neuroprotective effects. However, berberine has exhibited poor aqueous solubility and low oral bioavailability. To overcome these limitations, this study aims to develop and investigate nano berberine. Nano berberine fabricated by ball milling method is a simple process and easily applied in the production scale. The characterization of nano berberine was evaluated, including appearance, particle size, potential zeta, dissolution in vitro in a phosphate buffer media (pH 6.8), differential scanning calorimetry (DSC) analysis, fourier transformed infrared spectroscopy (FTIR), and X-ray diffraction analysis (XRD). Results from this study showed that spray-dried nano berberine powder was almost uniform in size, particle size average of 570.7 nm, and potential zeta of -28.6 mV. The dissolution in vitro study revealed that nano berberine was about 2 times more soluble than raw berberine for 60 minutes. No incompatibility between drug and excipients can be observed through FTIR and DSC analysis. XRD analysis suggested that raw berberine and nano berberine might exist in a crystal form. Thus, findings from this study indicate that the physicochemical properties of spray-dried nano berberine may increase the bioavailability of oral berberine. \u0000 ","PeriodicalId":23520,"journal":{"name":"VNU Journal of Science: Medical and Pharmaceutical Sciences","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77757456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. V. Trung, Nguyen Thu Hang, Duong Hong Anh, Pham Hung Viet
{"title":"Evaluation of the Hepatoprotective Activity of the Wood of Heliciopsis lobata (Merr.) Sleumer","authors":"B. V. Trung, Nguyen Thu Hang, Duong Hong Anh, Pham Hung Viet","doi":"10.25073/2588-1132/vnumps.4448","DOIUrl":"https://doi.org/10.25073/2588-1132/vnumps.4448","url":null,"abstract":"Heliciopsis lobata (Merr.) Sleumer is a popular herbal material in the North-West territories of Vietnam. The wood of this plant is still used for the treatment of some liver diseases, although there is no scientific evidence for evaluating these activities and the safety of this herbal material. Our study focused on accessing acute toxicity and hepatoprotective activity of water extract from the wood of Heliciopsis lobata. Among experimental groups of mice induced by high dosage of paracetamol, mice were treated with extract at dosages of 0.56 g/kg, 1.15 g/kg and 2.30 g/kg could be against liver damage with different levels. Water extract at dosages of 0.56 g/kg and 1.15 g/kg could maintain the original situation of livers; significantly reduced the increase of ALT and AST enzymes at levels of about 80% and 87% respectively; significantly reduced 70% the increase of AST, and against most of the peroxidation process in liver tissue. A dosage of 2.30 g/kg expressed lower hepatoprotective activities in comparison with the former dosages and which may increase liver toxicity when used with high dosages of paracetamol. The highest experimental dosage at 32.60 g/kg did not show acute toxicity by independent oral administration. It could be concluded that water extract of the wood of Heliciopsis lobata exposed hepatoprotective ability with dosage from 0.56 g/kg to 1.15 g/kg, equivalent to dosages from 60 g to 120 g for humans per day. \u0000Keywords: Hepatoprotective, ALT, AST, TBARs, Heliciopsis lobata, acute toxicity. \u0000 \u0000 \u0000 ","PeriodicalId":23520,"journal":{"name":"VNU Journal of Science: Medical and Pharmaceutical Sciences","volume":"93 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81479519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}