Virchows Archiv最新文献_第2页

Cancer-associated fibroblast marker signatures and stromal composition in usual interstitial pneumonia-associated lung adenocarcinoma: an analysis using a proteomic-immunohistochemical approach. 通常间质性肺炎相关肺腺癌中癌症相关成纤维细胞标志物特征和基质组成：使用蛋白质组免疫组织化学方法的分析

IF 3.1 3区医学

Virchows Archiv Pub Date : 2025-09-09 DOI: 10.1007/s00428-025-04254-8

Chihiro Inoue, Yasuhiro Miki, Tetsuya Fukuda, Yoshinori Okada, Hironobu Sasano, Takashi Suzuki

{"title":"Cancer-associated fibroblast marker signatures and stromal composition in usual interstitial pneumonia-associated lung adenocarcinoma: an analysis using a proteomic-immunohistochemical approach.","authors":"Chihiro Inoue, Yasuhiro Miki, Tetsuya Fukuda, Yoshinori Okada, Hironobu Sasano, Takashi Suzuki","doi":"10.1007/s00428-025-04254-8","DOIUrl":"https://doi.org/10.1007/s00428-025-04254-8","url":null,"abstract":"Lung adenocarcinoma (LUAD) associated with usual interstitial pneumonia (UIP) harbours distinct features compared to lung adenocarcinoma without UIP. Therefore, we aimed to characterise the tumour microenvironment of LUAD with UIP by focusing on cancer-associated fibroblasts (CAFs) and stromal composition. Immunohistochemistry was performed on 32 LUAD samples (16 each with and without UIP) to evaluate CAF marker expression and lymphocyte infiltration. Proteomic analysis of laser-microdissected stromal regions and reanalysis of publicly available single-cell RNA sequencing data were subsequently performed. LUAD with UIP had higher levels of podoplanin and collagen triple helix repeat containing 1 (CTHRC1) immunoreactivity, among the CAF markers examined in the fibrous stroma, increased inflammatory cell infiltration, and higher CD8 + and Foxp3 + lymphocyte counts than LUAD without UIP. The proteomic analysis revealed elevated aggrecan (ACAN) and hyaluronan and proteoglycan link protein 1 (HAPLN1) levels in the stroma of LUAD patients with UIP tissues, which was subsequently confirmed by immunohistochemistry. Single-cell RNA sequencing also supported ACAN and HAPLN1 expression in a subset of CAFs based on public data. The tumour microenvironment of LUAD in patients with UIP harbours distinct characteristics, including altered CAF markers and increased inflammatory cell infiltration in stromal components. Novel stromal proteins such as ACAN and HAPLN1 may play a role in the pathogenesis of LUAD with UIP.","PeriodicalId":23514,"journal":{"name":"Virchows Archiv","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BRAF positive Langerhans cell sarcoma arising from CALR positive myeloproliferative neoplasm: evidence of a clonal progenitor. 由CALR阳性骨髓增生性肿瘤引起的BRAF阳性朗格汉斯细胞肉瘤：克隆祖细胞的证据

IF 3.1 3区医学

Virchows Archiv Pub Date : 2025-09-08 DOI: 10.1007/s00428-025-04258-4

Zohre Sadeghian, Kirill A Lyapichev, Chieh Lin Fu, Gulrukh Botiralieva, Maria Julia Diacovo, Amir Behdad

引用次数: 0

Thymus-like phenotype in benign lymphoepithelial neoplasms of salivary glands: clinicopathological and molecular characterization and reappraisal of relationship to non-sebaceous lymphadenoma. 涎腺良性淋巴上皮肿瘤的胸腺样表型：临床病理和分子特征以及与非皮脂腺淋巴结瘤关系的重新评估。

IF 3.1 3区医学

Virchows Archiv Pub Date : 2025-09-06 DOI: 10.1007/s00428-025-04234-y

Abbas Agaimy, Jan Laco, Christoph Schubart, Robert Stoehr, Lars Tögel, Stephan Ihrler

{"title":"Thymus-like phenotype in benign lymphoepithelial neoplasms of salivary glands: clinicopathological and molecular characterization and reappraisal of relationship to non-sebaceous lymphadenoma.","authors":"Abbas Agaimy, Jan Laco, Christoph Schubart, Robert Stoehr, Lars Tögel, Stephan Ihrler","doi":"10.1007/s00428-025-04234-y","DOIUrl":"https://doi.org/10.1007/s00428-025-04234-y","url":null,"abstract":"Benign lymphoepithelial tumors of salivary glands had been restricted to sebaceous and non-sebaceous (NSLA) lymphadenomas. However, salivary neoplasms recapitulating carcinoma showing thymus-like elements (CASTLE) have been the subject of recent case reports. We reviewed clinicopathological, immunohistochemical, and molecular findings in 20 salivary gland tumors with thymus-like phenotype (18 histologically benign and two with malignant component). Original diagnoses were NSLA (n = 11) and unclassified thymus-like lymphoepithelial neoplasms (n = 9). Patients were 13 males and 7 females aged 28 to 83 years (median, 61). All tumors originated in the parotid with a median tumor size of 2.7 cm. A cystic component was noted in eight cases (40%). Histologically, the tumors were composed of large squamoid cells with indistinct cell borders, forming large irregular branching and anastomosing aggregates within lymphoid stroma with Hassall corpuscle-like structures and intraepithelial sprinkling of lymphocytes. All tumors were diffusely positive for p63/p40 and CK5/CK14. CD5 and CD117 were expressed in 13/20 (65%) and 15/19 (79%) cases, respectively. The malignant component in two cases showed lower CD5/CD117 expression. Targeted DNA sequencing revealed pathogenic/likely pathogenic CYLD mutations in 4/7 cases (57%). One case each had a mutation in TAF1 + WISP3, DNMT3A, and BCOR. Targeted RNA sequencing revealed a YAP1::MAML2 fusion in 1/7 cases. This is the first systematic study addressing the concept of thymus-like phenotype in benign lymphoepithelial salivary gland tumors, showing that the majority of NSLAs (65%) belong to this poorly characterized category. Frequent CYLD mutations in these histologically distinct tumors represent a novel addition to the spectrum of CYLD-mutated salivary neoplasms.","PeriodicalId":23514,"journal":{"name":"Virchows Archiv","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: The absence of FOXA2 and HNF1β expression in vasculogenic mesenchymal lesions of germ cell origin suggests an evolutionary pathway similar to that of sarcomatoid yolk sac tumor. 更正：FOXA2和HNF1β在生殖细胞来源的血管源性间充质病变中表达缺失，提示其进化途径与类肉瘤卵黄囊瘤相似。

IF 3.1 3区医学

Virchows Archiv Pub Date : 2025-09-05 DOI: 10.1007/s00428-025-04261-9

Costantino Ricci, Luisa Di Sciascio, Francesca Ambrosi, Marco Grillini, Veronica Mollica, Francesco Massari, Michelangelo Fiorentino, Antonio De Leo, Muhammad T Idrees, Thomas M Ulbright, Andres Martin Acosta

引用次数: 0

Implementation of IBD-DCA for ulcerative colitis: a single-institution experience. 溃疡性结肠炎IBD-DCA的实施：单一机构经验。

IF 3.1 3区医学

Virchows Archiv Pub Date : 2025-09-04 DOI: 10.1007/s00428-025-04239-7

David Egong, Michéal Hanly, Erinn McGrath, Maura B Cotter, Garret Cullen, Juliette Sheridan, Glen Doherty, Kieran Sheahan

{"title":"Implementation of IBD-DCA for ulcerative colitis: a single-institution experience.","authors":"David Egong, Michéal Hanly, Erinn McGrath, Maura B Cotter, Garret Cullen, Juliette Sheridan, Glen Doherty, Kieran Sheahan","doi":"10.1007/s00428-025-04239-7","DOIUrl":"https://doi.org/10.1007/s00428-025-04239-7","url":null,"abstract":"Ulcerative colitis (UC) is a clinicopathologically challenging diagnosis that greatly impacts patients' quality of life. The recently developed Inflammatory Bowel Disease-Distribution, Chronicity, Activity (IBD-DCA) scoring system offers histopathologists a consistent template to report colonic biopsies from UC patients in a standardised manner. We reviewed the rate of uptake of the IBD-DCA system within our department since its inception in 2022, and statistically correlated our histological findings with the corresponding UC Endoscopic Index of Severity (UCEIS). Of the 449 cases of IBD biopsies reported, 291 were established UC cases. The uptake of the IBD-DCA reporting format reached beyond 95% across all biopsy sites, with noted variation in applying the scoring system to rectal biopsies. Kendall's tau correlation of the 116 UC cases reported in the first six months with their UCEIS revealed a moderate positive correlation between histological activity and all components of the UCEIS (all τ > 0.260, p < 0.05). Possible explanations for histological-endoscopic discrepancies include non-representative endoscopic sampling, differences in detection threshold, and the pan-colonic nature of the UCEIS. We call for the application of the IBD-DCA in future clinical trials to predict possible relapses and guide treatment dosages.","PeriodicalId":23514,"journal":{"name":"Virchows Archiv","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the metastatic potential of isolated tumour cells and clusters-cords patterns of ISUP Grade 5 acinar adenocarcinoma of the prostate: a comprehensive morphological analysis. 探索ISUP 5级前列腺腺泡癌分离肿瘤细胞的转移潜力和团索模式：全面的形态学分析。

IF 3.1 3区医学

Virchows Archiv Pub Date : 2025-09-04 DOI: 10.1007/s00428-025-04253-9

Marina Valeri, Miriam Cieri, Matilde Pittarello, Vincenzo Belsito, Alessandra Bressan, Alessia Cimadamore, Grazia M Elefante, Vittorio Fasulo, Giovanni Lughezzani, Nicolò M Buffi, Rodolfo Hurle, Luigi M Terracciano, Piergiuseppe Colombo

{"title":"Exploring the metastatic potential of isolated tumour cells and clusters-cords patterns of ISUP Grade 5 acinar adenocarcinoma of the prostate: a comprehensive morphological analysis.","authors":"Marina Valeri, Miriam Cieri, Matilde Pittarello, Vincenzo Belsito, Alessandra Bressan, Alessia Cimadamore, Grazia M Elefante, Vittorio Fasulo, Giovanni Lughezzani, Nicolò M Buffi, Rodolfo Hurle, Luigi M Terracciano, Piergiuseppe Colombo","doi":"10.1007/s00428-025-04253-9","DOIUrl":"https://doi.org/10.1007/s00428-025-04253-9","url":null,"abstract":"Gleason pattern 5 (GP5) prostatic adenocarcinoma (PC) includes distinct morphologies: undifferentiated solid pattern (US), solid and cribriform with necrosis (CN), clusters and cords (CC), and isolated single tumour cells (ISTC). The role of these patterns in the metastatic setting is still poorly understood. We conducted a case-control retrospective histological characterization of two cohorts of ISUP Grade Group 5 PC, one with nodal metastases (N1) and one without (N0), comparing GP5 sub-patterns distribution, from robot-assisted radical prostatectomies with extended lymphadenectomy diagnosed between January 2013 and February 2023. A series of PC distant metastases was also retrieved and analyzed. The different GPs and their percentage were determined in lymph nodes, primary tumours, and distant metastases. A total of 88 PC N1, 70 PC N0, and 51 distant metastases were identified. Among the N1 cohort, GP5 was documented in 28/88 nodal metastases (32%), with US and CN documented in 16/88 (18%) and 9/88 (10%) cases, respectively. Overall, 79/88 patients had ISTC/CC in primary PC, but only 11 cases (14%) developed ISTC/CC in corresponding nodal metastases (p < 0.00001). In contrast, cribriform (CR) pattern was present in 75/88 lymph nodes (87%). In distant metastases, US predominated (27/51, 53%), while ISTC/CC were detected only in 6/51 cases (12%) and as a secondary pattern. Our results might suggest the existence of an intrinsic different metastatic potential of the ISTC/CC pattern, compared with CR or US, or of extrinsic environmental factors preventing their development in metastatic settings. These findings may support reconsideration of ISTC/CC as part of GP5.","PeriodicalId":23514,"journal":{"name":"Virchows Archiv","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

S100-positive stroma in salivary gland basal cell adenoma: recent progress and future perspectives. 涎腺基底细胞腺瘤中s100阳性基质的研究进展及展望

IF 3.1 3区医学

Virchows Archiv Pub Date : 2025-09-03 DOI: 10.1007/s00428-025-04240-0

Eiichi Sasaki, Katsuhiro Masago, Yasuko Fujita

引用次数: 0

Synchronous mucinous metaplasia and neoplasia of the ovarium and fallopian tube with STK11 and KRAS mutations: a case report. 伴有STK11和KRAS突变的卵巢和输卵管同步粘液化生和肿瘤：1例报告。

IF 3.1 3区医学

Virchows Archiv Pub Date : 2025-09-03 DOI: 10.1007/s00428-025-04236-w

Miroslava Flídrová, Eva Krkavcová, Nikola Hájková, Kristýna Němejcová, Pavel Dundr, Michaela Kendall Bártů

{"title":"Synchronous mucinous metaplasia and neoplasia of the ovarium and fallopian tube with STK11 and KRAS mutations: a case report.","authors":"Miroslava Flídrová, Eva Krkavcová, Nikola Hájková, Kristýna Němejcová, Pavel Dundr, Michaela Kendall Bártů","doi":"10.1007/s00428-025-04236-w","DOIUrl":"10.1007/s00428-025-04236-w","url":null,"abstract":"Synchronous mucinous metaplasia and neoplasia of the female genital tract (SMMN-FGT) is a rare disorder defined as mucinous lesions affecting at least two sites in the female genital tract. We report a case of SMMN-FGT in a Caucasian 65-year-old patient with a right adnexal mass. The patient underwent radical surgery and histological examination showed mucinous ovarian carcinoma combined with mucinous metaplasia of the fallopian tube. The carcinomatous infiltration also affected the left ovary, peritoneum, and omentum. Molecular analysis revealed a shared STK11 mutation in both the ovarian carcinoma and tubal metaplasia, and other mutations (including KRAS) that differed between these tissues. The patient received adjuvant chemotherapy combined with bevacizumab. As there is limited experience with SMMN-FGT, standard diagnostic and treatment protocols have not yet been established. Although association with Peutz-Jeghers syndrome (PJS) was described, our patient had no clinical signs of PJS and the detected STK11 mutation was likely somatic.","PeriodicalId":23514,"journal":{"name":"Virchows Archiv","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Skin manifestations uncover underlying Follicular helper T-cell lymphoma, angioimmunoblastic-type in an immunosuppressed patient. 免疫抑制患者的皮肤表现揭示了潜在的滤泡辅助t细胞淋巴瘤，血管免疫母细胞型。

IF 3.1 3区医学

Virchows Archiv Pub Date : 2025-09-03 DOI: 10.1007/s00428-025-04244-w

Marie-Laure García, Rebeca Manso, Juan Torre Castro, Lucía Pavía Pascual, Sergio Ramos Cillán, Daniel Morillo, Juan Carlos Caballero Hernaez, Fina Climent, Luis Requena, Socorro María Rodríguez Pinilla, Francisco Javier Díaz de la Pinta

{"title":"Skin manifestations uncover underlying Follicular helper T-cell lymphoma, angioimmunoblastic-type in an immunosuppressed patient.","authors":"Marie-Laure García, Rebeca Manso, Juan Torre Castro, Lucía Pavía Pascual, Sergio Ramos Cillán, Daniel Morillo, Juan Carlos Caballero Hernaez, Fina Climent, Luis Requena, Socorro María Rodríguez Pinilla, Francisco Javier Díaz de la Pinta","doi":"10.1007/s00428-025-04244-w","DOIUrl":"10.1007/s00428-025-04244-w","url":null,"abstract":"In the context of immunosuppression, most lymphoproliferative disorders are of B-cell origin and associated with Epstein-Barr virus (EBV). Follicular Helper T-cell Lymphoma, Angioimmunoblastic type (AITL) is the most frequently encountered T-cell lymphoma subtype. We present a challenging AITL case, initially misinterpreted as a polymorphic EBV + B-cell lymphoma, in a 62-year-old woman with a history of rheumatoid arthritis treated with multiple immunosuppressive agents. Over several years, she developed varied cutaneous manifestations with distinct histopathological infiltration patterns, prompting a diagnostic reassessment. Molecular studies demonstrated T-cell clonality, and RHOA and TET2 mutations, supporting the diagnosis of AITL with associated EBV + B-cell expansion. Notably, the presence of epidermotropic EBV + B-cell infiltrates masked the underlying T-cell neoplasm. This case underscores the importance of integrating histopathological, clinical, and molecular data, and highlights the diagnostic and biological complexity of AITL, particularly when it arises in the setting of immune dysregulation and exhibits features overlapping with EBV-driven B-cell proliferations.","PeriodicalId":23514,"journal":{"name":"Virchows Archiv","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel KIT mutation, D816_N819delinsll, in a patient with systemic mastocytosis: a case report. 新型KIT突变，D816_N819delinsll，在系统性肥大细胞增多症患者：一个病例报告。

IF 3.1 3区医学

Virchows Archiv Pub Date : 2025-09-02 DOI: 10.1007/s00428-025-04237-9

Hazem A Juratli, Hanna Wassmer, Darius Juskevicius, Ilaria Alborelli, Karin Hartmann, Alexandar Tzankov

引用次数: 0