Upsala journal of medical sciences最新文献

{"title":"Overview of approved COVID-19 vaccines in the EU, recommendations for use in Sweden and vaccine uptake over time: Report from the Swedish Medical Products Agency and the Public Health Agency of Sweden.","authors":"Björn Zethelius, Johanna Rubin, Nicklas Pihlström, Ulla Wändel Liminga, Helena Back, Bernice Aronsson, Anders Tegnell, Ulrika Marking, Jonas F Ludvigsson, Sören Andersson, Rickard Ljung","doi":"10.48101/ujms.v130.12937","DOIUrl":"10.48101/ujms.v130.12937","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this review is to describe the regulatory background of the COVID-19 vaccines, the national recommendations for use issued and vaccine uptake in Sweden. It includes an overview of licensing and relevant safety aspects identified by the European Medicines Agency (EMA) and the national vaccination plan issued by the Public Health Agency (PHA) of Sweden.</p><p><strong>Materials and methods: </strong>Information on dates of licensing and safety aspects of importance identified by EMA published on its website, was compiled and presented in a chronological order. National recommendations on COVID-19-vaccination and vaccinations-data on uptake and coverage using the national-vaccine-register are presented.</p><p><strong>Results: </strong>COVID-19 vaccines development, assessments using rolling review and licensing of the covid-19 vaccines was done in 2020 during less than a year. Large-scale production was implemented. Monthly safety reviews performed by the EMA identified risk for thrombosis with thrombocytopenia syndrome with adenoviral vaccines and myocarditis for mRNA vaccines which led to restrictions in national recommendations for specified groups.National vaccinations were launched in a phased manner during 2021. Persons of high age, risk groups and nursing home personnel were prioritised during primary vaccinations and for initial boosters. In the Swedish population, 85% recieved at least on vaccine dose from the age of 12. At least two doses were recieved by 81% from age 18 and 95% from age 80.</p><p><strong>Conclusion: </strong>Recommendations for national use adhered to relevant adverse drug reactions identified. The vaccine coverage was high. Timelines presented should be considered in follow-up studies of COVID-19-vaccines to manage possible selection bias and confounding.</p>","PeriodicalId":23458,"journal":{"name":"Upsala journal of medical sciences","volume":"69 ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12581643/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145446079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contraceptive choices and satisfaction: a cross-sectional analysis of sociodemographic influences.","authors":"Maria Åkesson, Jan Brynhildsen, Tanja Tydén, Niklas Envall, Helena Backman, Yvonne Skogsdal","doi":"10.48101/ujms.v130.12656","DOIUrl":"10.48101/ujms.v130.12656","url":null,"abstract":"<p><strong>Introduction: </strong>Investigating factors associated with contraceptive satisfaction is important to create a basis for tailored contraceptive counseling. In this study, we aimed to explore how sociodemographic characteristics affected women's level of satisfaction and choice of different contraceptive methods, using data collected during a randomized controlled trial (RCT) in the region Örebro County, Sweden.</p><p><strong>Methods: </strong>This cross-sectional study utilized data from a previously conducted RCT. Eligible participants were women aged 20-40 years who sought contraceptive counseling. All women who participated in the RCT and completed a follow-up questionnaire were included in the analysis.</p><p><strong>Results: </strong>Between February 2015 and March 2016, 1,946, participants were enrolled in the trial, with 1,198 (61.6%) completing the 2-month follow-up questionnaire. Overall, 81.3% of women reported being 'very satisfied' or 'satisfied' with their contraceptive method. Participants aged 27-40 years used long-acting reversible contraception (LARC) to a higher extent compared with those aged 20-26 years (adjusted odds ratio [aOR] 1.92, 95% confidence interval [CI] 1.44-2.56). Older age was associated with lower satisfaction (aOR 0.55, 95% CI 0.33-0.94).Participants with a body mass index (BMI) ≥ 25 more often used LARC (aOR 1.68, 95% CI 1.24-2.28) but were also more likely to report no use of contraceptives at all (aOR 1.56, 95% CI 1.01-2.43) compared with BMI < 25. The level of satisfaction tended to decrease with increasing BMI. Country of birth and educational level were not associated with satisfaction.</p><p><strong>Conclusions: </strong>The use of LARC was more common among women with BMI ≥ 25 and older women. While BMI, education, and place of birth did not affect satisfaction, women aged 27-40 reported lower satisfaction. These findings contrast with prior studies and highlight the complex sociodemographic influences on contraception experiences.</p>","PeriodicalId":23458,"journal":{"name":"Upsala journal of medical sciences","volume":"130 ","pages":"12656"},"PeriodicalIF":1.6,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12320922/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144785379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Avoidable injuries after intervention for abdominal aortic aneurysm: An analysis of negligence claims over 15 years in Sweden.","authors":"David Bergqvist, Pelle Gustafson, Larsolof Hafström","doi":"10.48101/ujms.v130.12171","DOIUrl":"10.48101/ujms.v130.12171","url":null,"abstract":"<p><strong>Background: </strong>Patients, who are subjected to a patient's injury, are legally allowed a compensation for their suffering.</p><p><strong>Aim: </strong>The negligence claims after surgical treatment of abdominal aortic aneurysms (AAA) registered in the National Swedish patient insurance company (Landstingens Ömsesidiga Försäkringsbolag [LÖF]) between 2006 and 2020 were analyzed. More than 95% of negligence claims are covered by LÖF. Special emphasis on avoidable or unavoidable injuries was made.</p><p><strong>Material: </strong>In 15 years 17,000 abdominal aortic interventions were recorded in the Swedish vascular register (SWEDVASC), where vascular interventions in the whole country of Sweden are registered. A total of 151 negligence claims (0.9%) were reported to the insurance company. Available clinical information in the company's file of the claims was analyzed. The SWEDVASC data on AAA treatment were accessible.</p><p><strong>Results: </strong>The number of Endovascular repair (EVAR) increased significantly, but the total number of interventions decreased. There were less claims after EVAR (0.7%) compared to open surgery (1.1%). There was an increase in avoidable injuries that were economically compensated (<i>p</i> = 0.02). Spinal cord ischemia and intestinal ischemia were dominating causes for claims.</p><p><strong>Conclusion: </strong>The increase in the number of <i>avoidable</i> injuries should have an impact on how to train and support colleagues under education and efforts to diminish the injuries are essential. To develop methods to diminish the risk for <i>non-avoidable</i> complications is important.</p>","PeriodicalId":23458,"journal":{"name":"Upsala journal of medical sciences","volume":"130 ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12320923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144785378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eating patterns in relation to anthropometrics and blood pressure among adults with overweight and obesity - a cross-sectional study.","authors":"Elin Siurua, Kjell-Åke Alle, Mari Bergenholtz, Lena Lendahls, Sara Holmberg","doi":"10.48101/ujms.v130.12227","DOIUrl":"10.48101/ujms.v130.12227","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to describe eating patterns among individuals with overweight and obesity and to investigate associations between eating patterns and anthropometric measures, including body mass index (BMI) and waist circumference, and blood pressure.</p><p><strong>Methods: </strong>This study enrolled a cohort of adults with overweight or obesity (<i>n</i> = 176) participating in a clinical trial focused on weight reduction. Self-reported eating patterns were assessed as part of the trial's baseline survey. Trained study nurses conducted measurements of anthropometric indicators and blood pressure. To examine associations, statistical analyses included the application of the Mann-Whitney U-test, Fisher's exact test, the chi-squared test, and linear regression models as appropriate.</p><p><strong>Results: </strong>The median age of the participants was 55 years (interquartile range [IQR] 12), 79% were female, and the median BMI was 33 kg/m² (IQR 5). The predominant eating pattern identified was characterized by five meals per day, including breakfast, two prepared meals, and two snacks. Among older participants (≥ 55 years), 51% reported eating two prepared meals per day as compared to 75% among the younger (<i>P</i> <> 0.05). A higher percentage of older participants reported consuming more than one snack per day (82% vs. 68%, <i>P</i> = 0.04). Additionally, older participants were more likely to rate their eating habits as 'good' compared to their younger counterparts (64% vs. 52%, <i>P</i> = 0.03). Women reported a higher number of eating occasions than men (> 3/day: 93% vs. 78%, <i>P</i> = 0.01) and a higher frequency of snacks (> 1 snack/day: 79% vs. 61%, <i>P</i> = 0.03). No significant associations between the number of eating occasions or number of snacks and BMI, waist circumference, or blood pressure (systolic and/or diastolic) were found in regression models when age and sex were considered.</p><p><strong>Conclusions: </strong>Varying eating patterns were observed among adults with overweight and obesity according to age and sex. No association between eating patterns and anthropometric measures or blood pressure independent of age and sex was found.</p>","PeriodicalId":23458,"journal":{"name":"Upsala journal of medical sciences","volume":"130 ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12320924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144785380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ARDS severity in COVID-19: a case-control study of laboratory biomarkers and IL-10 SNP analysis.","authors":"Shukur Wasman Smail, Niaz Albarzinji, Karim Jalal Karim, Rebaz Hamza Salih, Christer Janson","doi":"10.48101/ujms.v130.11515","DOIUrl":"10.48101/ujms.v130.11515","url":null,"abstract":"<p><strong>Background: </strong>Acute respiratory distress syndrome (ARDS), which is often observed in severe cases of coronavirus disease 2019 (COVID-19), is known to be a major contributor to higher mortality rates. This study assesses how hematological parameters, inflammatory biomarkers, cytokines, and the -1,082 A/G polymorphism are associated with ARDS severity in COVID-19 patients.</p><p><strong>Methods: </strong>Following exclusions, a 6-month prospective case-control study included 82 healthy controls (HCs) and 158 COVID-19 patients with varying severities of ARDS (mild: 73, moderate: 53, and severe: 32). Blood samples were collected at admission, and laboratory biomarkers were assessed using various methods. Statistical analyses included one-way analysis of variance with Tukey's test for group comparisons, Pearson correlation, and receiver operating characteristic curve for analyzing independent associations with COVID-19 severity. Multiple linear regression and chi-square tests were used to evaluate quantitative outcomes and categorical associations, respectively.</p><p><strong>Results: </strong>Severe ARDS patients exhibited higher C-reactive protein (CRP) levels compared to HCs. Compared to HCs, patients with moderate and severe ARDS had higher neutrophil to lymphocyte ratio (NLR), neutrophil counts, tumor necrosis factor-alpha, and interleukin-10 (IL-10), as well as lower lymphocyte counts and reduced partial pressure of oxygen/fraction of inspired oxygen (PaO₂/FiO₂) ratio. IL-10, body mass index, CRP, and NLR were associated with reduced PaO₂/FiO₂ ratio. IL-10 and CRP had the highest area under curve values toward ARDS severity. COVID-19 patients possessing the -1,082 A/G single nucleotide IL-10 GG and GA genotypes and the G allele presented with less severe ARDS.</p><p><strong>Conclusion: </strong>Hematological indices (neutrophil count and NLR), CRP, and serum IL-10 hold promise in monitoring ARDS severity in COVID-19 patients. In addition, COVID-19 patients with GG and AG genotypes and the G allele of the IL-10 gene's-1,082 A/G polymorphism experience less severe ARDS. This highlights the potential protective role of IL-10 genetic variation in modulating the severity of inflammatory responses during severe acute respiratory syndrome-coronavirus-2 infection and may serve as a useful genetic marker for risk stratification in clinical settings.</p>","PeriodicalId":23458,"journal":{"name":"Upsala journal of medical sciences","volume":"130 ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12320925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144785377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toll-like receptor 4 (TLR-4) polymorphisms and asthma risk in rural and urban settings: findings from the UK biobank.","authors":"Marta A Kisiel, Mathias Rask-Andersen, Åsa Johansson, Weronica E Ek, Anna Rask-Andersen","doi":"10.48101/ujms.v130.12243","DOIUrl":"10.48101/ujms.v130.12243","url":null,"abstract":"<p><strong>Introduction and aim: </strong>The risk of asthma and its phenotypes may be modified by gene-environmental interactions. The previous studies on the interactions between genetic variations in the toll like 4 (TLR4)<i>,</i> the main receptor for bacterial endotoxin, and asthma were contradictory as they were underpowered and did not consider different asthma phenotypes. The main aim of this study was to identify interactions between two single nucleotide polymorphisms (SNPs) within the <i>TLR4</i> gene, Asp299Gly and Thr399Ile, and residential area (urban or rural) in females and males with asthma and different asthma phenotypes.</p><p><strong>Method: </strong>This study was performed on 38,332 asthmatics and 322,852 non-asthma (both British Caucasians) subjects from the UK Biobank database. Asthma was also divided into phenotypes, such as asthma with/without allergy and early/late onset asthma. The residential area was based on the population area density and classified as urban or rural living. Multivariate regression models adjusted for age, body mass index, and smoking status were used to analyze interactions between the SNPs, residential area in asthma, and asthma phenotypes. The association between asthma and residential area or the SNPs was also determined.</p><p><strong>Result: </strong>There were no significant associations between the SNPs and asthma risk (for Asp299Gly: OR (95% CI): 1.00 (0.97-1.02), for Thr399Ile: 0.99 (0.96-1.02) or between the SNPs and asthma phenotypes in either sex or combined cohorts. The effects of the SNPs were not modified by residential area population density in either sex with asthma or across asthma phenotypes. Asthma and its phenotypes were not associated with the SNPs or residential area.</p><p><strong>Conclusions: </strong>Our study found no statistically significant association between <i>TLR4</i> polymorphisms and asthma, regardless of sex or residential area. Further studies are needed to clarify the functional impact of TLR4 variation in asthma pathophysiology.</p>","PeriodicalId":23458,"journal":{"name":"Upsala journal of medical sciences","volume":"130 ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12320926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144785381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations of lower values of peak oxygen uptake and handgrip strength with a smaller liver volume.","authors":"Muhammad Naeem, Marcello Ricardo Paulista Markus, Martin Bahls, Mohammed Mousa, Marcus Dörr, Jens-Peter Kühn, Robin Bülow, Stephan B Felix, Giovanni Targher, Beate Stubbe, Ralf Ewert, Henry Völzke, Till Ittermann","doi":"10.48101/ujms.v130.11924","DOIUrl":"10.48101/ujms.v130.11924","url":null,"abstract":"<p><strong>Background and aims: </strong>The associations between physical fitness markers and liver volume in the general population are unclear. We investigated the associations of peak oxygen uptake (VO_2peak)and handgrip strength with liver volume in a general population sample.</p><p><strong>Methods and results: </strong>Data were taken from 1,531 German adults (51.3% women), aged 20 to 88 years, from two cohorts of the population-based Study of Health in Pomerania (SHIP-START-2 and SHIP-TREND-0). We analysed cross-sectional associations of VO_2peak and handgrip strength with liver volume derived from magnetic resonance imaging (MRI) by using multivariable linear regression models. These models were adjusted for age, sex, body fat mass, pre-existing type 2 diabetes, daily alcohol consumption, smoking status, and use of hypoglycaemic or antihypertensive medications. We observed significant associations of lower VO_2peak and handgrip strength with a smaller liver volume in the whole population, as well as in both men and women. In the whole population, a 1 L/min lower VO_2peak was associated with a 0.15 cm³ (95% confidence interval [CI]: 0.11 to 0.19; <i>P</i> < 0.0001) smaller liver volume for both sexes together. Similarly, a 1 kg lower handgrip strength was associated with a 7.05 cm³ (95% CI: 4.87 to 9.23; <i>P</i> < 0.001) smaller liver volume in the whole population.</p><p><strong>Conclusion: </strong>Our results derived from a large community-based sample showed that lower values of VO_2peak and handgrip strength were associated with a smaller liver volume. These results might explain the possible negative effects of sedentary lifestyle on liver volume - the sedentary liver.</p>","PeriodicalId":23458,"journal":{"name":"Upsala journal of medical sciences","volume":"130 ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12138976/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144235362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cost-effectiveness analysis of transcatheter aortic valve implantation versus surgical aortic valve replacement in patients with severe aortic stenosis at low risk of surgical mortality in Sweden.","authors":"Konrad Nilsson, Stefan James, Oskar Angerås, Jenny Backes, Henrik Bjursten, Pascal Candolfi, Mattias Götberg, Henrik Hagström, Chiara Malmberg, Niels Erik Nielsen, Archita Sarmah, Magnus Settergren, Tom Bromilow","doi":"10.48101/ujms.v130.10741","DOIUrl":"10.48101/ujms.v130.10741","url":null,"abstract":"<p><strong>Background: </strong>Transcatheter aortic valve implantation (TAVI) has shown similar or improved clinical outcomes compared with surgical aortic valve replacement (SAVR) in patients with symptomatic severe aortic stenosis at low risk for surgical mortality. This cost-utility analysis compared TAVI with SAPIEN 3 versus SAVR in symptomatic severe aortic stenosis patients at low risk of surgical mortality from the perspective of the Swedish healthcare system.</p><p><strong>Methods: </strong>A published, two-stage, Markov-based cost-utility model that captured clinical outcomes from the <i>Swedish Web-system for Enhancement and Development of Evidence-based care in Heart disease Evaluated according to Recommended Therapies</i> (SWEDEHEART) registry (2018-2020) was adapted from the perspective of the Swedish healthcare system using local general population mortality, utility and costs data. The model had a lifetime horizon. Model outputs included changes in direct healthcare costs and health-related quality of life from using TAVI as compared with SAVR.</p><p><strong>Results: </strong>TAVI with SAPIEN 3 resulted in lifetime costs per patient of 484,142 SEK Swedish krona (SEK) and lifetime quality-adjusted life years (QALYs) per patient of 7.16, whilst SAVR resulted in lifetime costs and QALYs per patient of 457,625 SEK and 6.81 QALYs, respectively. Compared with SAVR, TAVI offered an incremental improvement of +0.35 QALY per patient at an increased cost of +26,517 SEK per patient over a lifetime horizon, resulting in an incremental cost-effectiveness ratio of 76,532 SEK per QALY gained.</p><p><strong>Conclusion: </strong>TAVI with SAPIEN 3 is a cost-effective option versus SAVR for patients with symptomatic severe aortic stenosis at low risk for surgical mortality treated in the Swedish healthcare setting. These findings may inform policy decisions in Sweden for the management of this patient group.</p>","PeriodicalId":23458,"journal":{"name":"Upsala journal of medical sciences","volume":"130 ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12045074/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144053337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CORRIGENDUM.","authors":"","doi":"10.48101/ujms.v130.12186","DOIUrl":"https://doi.org/10.48101/ujms.v130.12186","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.48101/ujms.v129.10741.].</p>","PeriodicalId":23458,"journal":{"name":"Upsala journal of medical sciences","volume":"130 ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12045073/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144048454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elevated levels of aquaporin-4-containing extracellular vesicles in cerebrospinal fluid of patients with bipolar disorder.","authors":"Lennart Wetterberg, Fariborz Mobarrez, Rolf Nybom, Håkan Wallén, Aurimantas Pelanis, Dietrich von Rosen, Mikael Landén","doi":"10.48101/ujms.v130.12006","DOIUrl":"10.48101/ujms.v130.12006","url":null,"abstract":"<p><strong>Objectives: </strong>To examine a hypothetical dysfunction of the brain water channels in bipolar disorder by analyzing aquaporin-4 (AQP4) exposing extracellular vesicles (EVs) in cerebrospinal fluid (CSF) from individuals with bipolar disorder types 1 and 2, and healthy controls.</p><p><strong>Methods: </strong>We analyzed exposure of AQP4 EVs to three different epitopes - the N- and C-terminals, and the epitope containing amino acids 273-291 - in CSF by flow cytometry in 24 individuals with bipolar disorder (type 1, <i>n</i> = 20; type 2, <i>n</i> = 4) and in 14 healthy controls.</p><p><strong>Results: </strong>We observed significantly higher levels of EVs expressing AQP4 in the CSF from individuals with bipolar disorder compared with healthy controls. Specifically, the mean ± SD concentration of AQP4 + EVs per μl CSF for the N-terminal epitope was 346 ± 22 in patients with bipolar disorder type 1, 386 ± 78 in those with bipolar disorder type 2, compared with 39 ± 6.9 in the healthy control group (<i>P</i> < 0.0001). For AQP4+ EVs targeting the C-terminal epitope, the corresponding values were 350 ± 22 for bipolar disorder type 1, 374 ± 46 for bipolar disorder type 2, and 36 ± 6.3 for healthy controls. Similarly, EVs expressing AQP4+ epitopes containing amino acids 273-291 showed concentrations of 344 ± 17 in bipolar disorder type 1, 398 ± 63 in bipolar disorder type 2, and 38 ± 6.4 in the control group (<i>P</i> < 0.0001).</p><p><strong>Conclusion: </strong>Our findings revealed significantly more EVs expressing the three AQP4 epitopes in patients with bipolar disorder compared with healthy controls. This suggests a dysregulated expression of AQP4, implicating a potential disruption in brain water homeostasis as a contributing pathogenic mechanism in bipolar disorder.</p>","PeriodicalId":23458,"journal":{"name":"Upsala journal of medical sciences","volume":"130 ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12292035/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}