Tuberculosis最新文献

{"title":"The first probable case with tuberculous meningitis from the Hun period of the Carpathian Basin – How diagnostics development can contribute to increase knowledge and understanding of the spatio-temporal distribution of tuberculosis in the past","authors":"Olga Spekker ,&nbsp;Luca Kis ,&nbsp;Nikoletta Lukács ,&nbsp;Eszter Patyi ,&nbsp;Balázs Tihanyi","doi":"10.1016/j.tube.2023.102372","DOIUrl":"https://doi.org/10.1016/j.tube.2023.102372","url":null,"abstract":"<div><p><span>The aim of our paper is to demonstrate and discuss in detail the endocranial bony changes suggestive of tuberculous meningitis (TBM) that were recorded in an adult female's (</span><strong>SPF15</strong><span>) skeleton. The bone remains were uncovered from a solitary grave from the Hun period (5th-century-CE) archaeological site of Solt–Polya-fok (Bács-Kiskun county, Hungary). During the macromorphological examination of the very incomplete and poorly preserved skeleton of </span><strong>SPF15</strong>, the inner surface of the skull displayed abnormally pronounced digital impressions (APDIs) and granular impressions (GIs). Recently, it was confirmed that endocranial GIs can be considered as specific signs of TBM; and thus, they are sufficient enough on their own to make a definitive diagnosis of the disease in the palaeopathological practice. On the other hand, APDIs are not specific to TBM but can be tuberculous in origin; their concomitant presence with GIs in <strong>SPF15</strong> makes their tuberculous origin very likely. Based on the above, it seems that the adult female from the 5th-century-CE archaeological site of Solt–Polya-fok suffered from TBM. <strong>SPF15</strong> is the first reported probable case with tuberculosis (TB) from the Hun period of the present-day territory of Hungary, who gives us invaluable information about the spatio-temporal distribution of the disease in the past. Furthermore, it highlights the paramount importance of diagnostics development, especially the identification and refinement of diagnostic criteria, as without the application of APDIs and GIs, the diagnosis of TB could not have been established in <strong>SPF15</strong>.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"143 ","pages":"Article 102372"},"PeriodicalIF":3.2,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138438175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Re-examination of the Subalyuk Neanderthal remains uncovers signs of probable TB infection (Subalyuk Cave, Hungary)","authors":"György Pálfi ,&nbsp;Erika Molnár ,&nbsp;Zsolt Bereczki ,&nbsp;Hélène Coqueugniot ,&nbsp;Olivier Dutour ,&nbsp;Anne-marie Tillier ,&nbsp;Wilfried Rosendahl ,&nbsp;Antal Sklánitz ,&nbsp;Zsolt Mester ,&nbsp;Mihály Gasparik ,&nbsp;Frank Maixner ,&nbsp;Albert Zink ,&nbsp;David E. Minnikin ,&nbsp;Ildikó Pap","doi":"10.1016/j.tube.2023.102419","DOIUrl":"https://doi.org/10.1016/j.tube.2023.102419","url":null,"abstract":"<div><p><span>In 1932, skeletal remains of two Neanderthal individuals, a young adult female and a 3–4–year-old child, were discovered in Subalyuk Cave in Northern Hungary [1,2]. Results of the anthropological examination were published some years after this important discovery. Methodological progress encouraged re-examination of the material during the last few years. Radiocarbon dating revealed a chronological age of 39,732–39,076 cal. BP for the adult female and 36,117–35,387 cal. BP for the child [3]. Morphological paleopathological studies of these Neanderthal remains uncovered distinct evidence of skeletal infections. Alterations of the adult individual's </span><span><em>sacrum</em></span> suggest probable early-stage <span><em>sacroiliitis</em></span><span>, while several vertebral bodies indicate superficial osseous remodelling of infectious origin. Traces of pathological lesions were observed on the endocranial surface of the child's skull, reflecting a reaction of meningeal tissues, a consequence of a probable TB-related meningeal infectious process. Results of recent paleomicrobiological examinations – lipid biomarker and aDNA studies – support the morphological diagnosis of probable TB infections [4].</span></p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"143 ","pages":"Article 102419"},"PeriodicalIF":3.2,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138437699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Professor David Minnikin Memorial Lecture: An era of the mycobacterial cell wall lipid biomarkers","authors":"Oona Y-C Lee ,&nbsp;Houdini H.T. Wu ,&nbsp;Gurdyal S. Besra","doi":"10.1016/j.tube.2023.102415","DOIUrl":"https://doi.org/10.1016/j.tube.2023.102415","url":null,"abstract":"<div><p>This paper is dedicated to the memory of Professor David Ernest Minnikin (1939–2021). David was one of the key scientists who pioneered the field of <em>Mycobacterium tuberculosis</em> cell envelope research for over half a century. From the classification, identification, and extraction of the unusual lipids of the mycobacterial cell wall, to exploiting them as characteristic lipid biomarkers for sensitive detection, his ideas enlightened a whole world of possibilities within the tuberculosis (TB) field. In addition, his definition of the intricate models now forms a key milestone in our understanding of the <em>M</em>. <em>tuberculosis</em> cell envelope and has resolved many unanswered questions on the evolution of <em>M. tuberculosis</em>.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"143 ","pages":"Article 102415"},"PeriodicalIF":3.2,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1472979223001130/pdfft?md5=8477bf506bcb078239d8a0633f6931fb&pid=1-s2.0-S1472979223001130-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138437782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hit or miss - A metagenomic evaluation of intra-bone variability of host pathogen load in tuberculosis-infected human remains","authors":"Heidi Y. Jäger ,&nbsp;Daniel Atz Zanotelli ,&nbsp;Frank Maixner ,&nbsp;Nicole Nicklisch ,&nbsp;Kurt W. Alt ,&nbsp;Harald Meller ,&nbsp;Ildikó Pap ,&nbsp;Ildikó Szikossy ,&nbsp;György Pálfi ,&nbsp;Albert R. Zink","doi":"10.1016/j.tube.2023.102392","DOIUrl":"https://doi.org/10.1016/j.tube.2023.102392","url":null,"abstract":"<div><p><span><span>Many sampling protocols have been established to successfully retrieve human DNA from archaeological remains, however the systematic detection of ancient pathogens remains challenging. Here, we present a first assessment of the intra-bone variability of </span>metagenomic composition in human skeletal remains and its effect on the sampling success for </span><span><em>Mycobacterium tuberculosis</em></span><span> (MTB) and human endogenous DNA. For this purpose, four bone samples from published peer-reviewed studies with PCR-based evidence for ancient MTB DNA were selected. Two bone samples of a Neolithic individual from Halberstadt, Germany and two ribs of two 18th-century Hungarian church mummies were sampled at multiple locations for equal amounts, followed by DNA extraction and library construction. Shotgun sequencing data was generated for taxonomic profiling as well as quantitative and qualitative evaluation of MTB and human endogenous DNA. Despite low variance in microbial diversity within and across samples, intra-bone variability of up to 36.45- and 62.88-fold for authentic ancient MTB and human reads, respectively, was detected. This study demonstrates the variable sampling success for MTB and human endogenous DNA within single skeletal samples despite relatively consistent microbial composition and highlights how a multisampling approach can facilitate the detection of hotspots with highly concentrated pathogen and human endogenous DNA.</span></p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"143 ","pages":"Article 102392"},"PeriodicalIF":3.2,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138438165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards a better understanding of the long-lasting evolutionary history of Mycobacterium tuberculosis","authors":"Gaetan Senelle ,&nbsp;Christophe Guyeux ,&nbsp;Guislaine Refrégier ,&nbsp;Christophe Sola","doi":"10.1016/j.tube.2023.102374","DOIUrl":"https://doi.org/10.1016/j.tube.2023.102374","url":null,"abstract":"<div><p>The daily increasing sequencing of <span><em>Mycobacterium tuberculosis</em></span><span> has made it possible to establish an advanced phylogeny of this bacterium. It currently includes 9 lineages mainly affecting humans, completed by animal lineages, which form the </span><em>Mycobacterium tuberculosis</em><span> complex. Inherited from various historical approaches, this phylogeny is now based on Single Nucleotide Polymorphisms (SNPs), of which updates are frequently proposed. We present here evidence that the task needs refinements: some lineages have currently suboptimal defining SNPs, and many sublineages still need to be named and characterized. These findings are based on a new tool specifically designed to index the entire existing sequencing data. In this article, we focus on lineages 4.5, 4.7, 6 and 7. We take the opportunity to present some evidence that TB-annotator shows strong relevance, identifying well supported sublineages, as well as good global agreement with previous findings.</span></p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"143 ","pages":"Article 102374"},"PeriodicalIF":3.2,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138438174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamics of tuberculosis infection in various populations during the 19th and 20th century: The impact of conservative and pharmaceutical treatments","authors":"K.L. Holloway-Kew ,&nbsp;M. Henneberg","doi":"10.1016/j.tube.2023.102389","DOIUrl":"https://doi.org/10.1016/j.tube.2023.102389","url":null,"abstract":"<div><p>Humans and <em>Mycobacterium tuberculosis</em> have co-evolved together for thousands of years. Many individuals are infected with the bacterium, but few show signs and symptoms of tuberculosis (TB). Pharmacotherapy to treat those who develop disease is useful, but drug resistance and non-adherence significantly impact the efficacy of these treatments. Prior to the introduction of antibiotic therapies, public health strategies were used to reduce TB mortality. This work shows how these strategies were able to reduce TB mortality in 19th and 20th century populations, compared with antibiotic treatments.</p><p>Previously published mortality data from historical records for several populations (Switzerland, Germany, England and Wales, Scotland, USA, Japan, Brazil and South Africa) were used. Curvilinear regression was used to examine the reduction in mortality before and after the introduction of antibiotic treatments (1946).</p><p>A strong decline in TB mortality was already occurring in Switzerland, Germany, England and Wales, Scotland and the USA prior to the introduction of antibiotic treatment. This occurred following many public health interventions including improved sanitation, compulsory reporting of TB cases, diagnostic techniques and sanatoria treatments. Following the introduction of antibiotics, mortality rates declined further, however, this had a smaller effect than the previously employed strategies. In Japan, Brazil and South Africa, reductions in mortality rates were largely driven by antibiotic treatments that caused rapid decline of mortality, with a smaller contribution from public health strategies.</p><p>For the development of active disease, immune status is important. Individuals infected with the bacterium are more likely to develop signs and symptoms if their immune function is reduced. Effective strategies against TB can therefore include enhancing immune function of the population by improving nutrition, as well as reducing transmission by improving living conditions and public health. This has been effective in the past. Improving immunity may be an important strategy against drug resistant TB.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"143 ","pages":"Article 102389"},"PeriodicalIF":3.2,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1472979223000872/pdfft?md5=3b4bd5aa6b8baf075326140a377e579e&pid=1-s2.0-S1472979223000872-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138437678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aims and Scope, Copyright, Publication information, Orders and Claims, Advertising information, Author inquiries, Permissions, Funding body, Permanence of paper, Impressum (German titles only) and GFA link in double column","authors":"","doi":"10.1016/S1472-9792(23)00161-0","DOIUrl":"https://doi.org/10.1016/S1472-9792(23)00161-0","url":null,"abstract":"","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"143 ","pages":"Article 102449"},"PeriodicalIF":3.2,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138437737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"\"Tuberculosis on the spot\" – Discussion of a probable sternal tuberculosis from a late medieval cemetery from Hungary","authors":"Csilla Libor ,&nbsp;Tamás Hajdu ,&nbsp;Tamás Szeniczey ,&nbsp;Loránd O. Kovács ,&nbsp;László Kunos ,&nbsp;Orsolya Mateovics-László","doi":"10.1016/j.tube.2023.102410","DOIUrl":"https://doi.org/10.1016/j.tube.2023.102410","url":null,"abstract":"<div><p><span><span>Investigations of non-adult remains are particularly suitable for finding epidemic periods in past populations. This study presents a probable unique example of osseous manifestation of tuberculosis on a child's skeletal remains from medieval Hungary. Between 2009 and 2011 the Field Service for Cultural Heritage excavated the exceptional cemetery of Perkáta – Nyúli-dűlő in Hungary, with around 5000+ graves. The analysed skeleton<span><span> (SNR 948) was located in the medieval (10–16th century) part of the cemetery. Besides the standard macroscopic pathological observation, we also performed radiographic analysis. The remains of the child (13-14 year-old) showed numerous skeletal lesions: the ribs have proliferative lesions (dense nodules) on the visceral surface of the shaft, lytic lesions with rounded edges occurred on the thoracic and </span>lumbar </span></span>vertebral bodies<span>, and on the facies auricularis of the left ilium we can see pitting and new bone formation. What makes this pathological case exceptional is the significant change in the </span></span>manubrium<span>. It shows extensive osteolytic lesions<span><span>, probably due to tuberculous osteomyelitis, which is a unique phenomenon in an archaeological context. This rare type of extra-spinal tuberculous osteomyelitis appears in less than 1% of cases with </span>skeletal TB, and even less in case of children, according to modern medical literature. Although some cases of slight lesions on the manubrium have been described from an archaeological context, no such cases showing advanced lesions have been published so far. In the future, biomolecular analyses should be conducted as well, in order to confirm the presence of TB in this individual.</span></span></p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"143 ","pages":"Article 102410"},"PeriodicalIF":3.2,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138437826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activity induced worsening of a tuberculous process in the vertebral column of a Late Bronze Age female from Liushui, Xinjiang (China)?","authors":"Michael Schultz ,&nbsp;Jeannine Missbach-Guentner ,&nbsp;Mayke Wagner ,&nbsp;Pavel Tarasov ,&nbsp;Julia Gresky ,&nbsp;Tyede H. Schmidt-Schultz","doi":"10.1016/j.tube.2023.102370","DOIUrl":"https://doi.org/10.1016/j.tube.2023.102370","url":null,"abstract":"<div><p><span><span><span>The partial skeleton of a 22-24-year old female from Liushui, Southern Silk Road, Xinjiang (China) was analyzed using morphological and biochemical methods. The most striking finding in this individual of a Late Bronze Age mounted nomadic population was the complete ossification of the caudal </span>vertebral column<span> including parts of the ligaments of this region due to chronic tuberculosis (Pott's disease). The morphological diagnosis is definitely confirmed by the results of the proteomic analysis. The </span></span>bacterial protein Ag85 and, for the first time in archaeological skeletal remains, also ESAT-6 was detected, which are typical for </span><span><em>Mycobacterium tuberculosis</em></span><span><span>. Extremely intense physical stress aggravated the pathological kyphosis primarily caused by the tuberculous process and promoted dislocation of the caudal thoracic versus the </span>lumbar vertebrae. The fate of this young female suffering from tuberculosis and the consequences of this extreme physical stress characterize the harsh living conditions of typical prehistoric population of mounted nomadic pastoralists.</span></p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"143 ","pages":"Article 102370"},"PeriodicalIF":3.2,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138437662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tuberculosis in mummies – New findings, perspectives and limitations","authors":"Albert Zink ,&nbsp;Frank Maixner ,&nbsp;Heidi Yoko Jäger ,&nbsp;Ildikó Szikossy ,&nbsp;György Pálfi ,&nbsp;Ildikó Pap","doi":"10.1016/j.tube.2023.102371","DOIUrl":"https://doi.org/10.1016/j.tube.2023.102371","url":null,"abstract":"<div><p><span>The molecular analysis of ancient pathogen DNA represents a unique opportunity for the study of infectious diseases in ancient human remains. Among other diseases, paleogenetic studies have been successful in detecting tuberculous DNA in ancient human remains. In the beginning of ancient DNA (aDNA) studies, the presence of tuberculosis (TB) DNA was assessed using a PCR-based assay targeting specific regions of the </span><span><em>Mycobacterium tuberculosis</em></span> (MTB) complex, such as the repetitive element IS6110. The advent of high-throughput sequencing has enabled the reconstruction of full ancient TB genomes in the field of paleomicrobiology. However, despite the numerous paleopathological and PCR-based studies on the presence of tuberculosis in historic human remains, full genome wide reconstructions are still limited to well-preserved specimens with low environmental contamination and connected with extensive screening efforts. This has led to some controversies regarding the evolutionary history of its causative agent <em>Mycobacterium tuberculosis.</em><span> In this context, mummies have been shown to be a good source for the detection of MTB complex DNA due to a low exposure to environmental influences and the overall good state of preservation of hard and soft tissues in the human remains. Here, we present the major findings on the presence of TB infections in the 18th century naturally mummified human remains from Vác, Hungary and the current status of the detection of MTB complex DNA in mummified human remains. The future perspectives of detecting tuberculosis in mummies will be discussed in the light of methodological aspects, as well as ethical and curational challenges.</span></p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"143 ","pages":"Article 102371"},"PeriodicalIF":3.2,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138437697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}