Tropical animal health and production最新文献

{"title":"Effect of probiotic supplementation on productive performance and epithelial intestinal integrity of broiler chickens exposed to heat stress.","authors":"Ana Cecilia Hernández-Coronado, Miguel Cervantes, Fernanda González, Alan Valle, Nestor Arce, Nydia Vásquez, Hugo Bernal, Adriana Morales","doi":"10.1007/s11250-025-04488-3","DOIUrl":"https://doi.org/10.1007/s11250-025-04488-3","url":null,"abstract":"<p><p>Heat stress (HS) impacts performance and intestinal homeostasis of broiler chickens. Probiotic represents an alternative to counteract those negative effects. This study evaluated the performance and intestinal integrity of HS chickens supplemented with a B. subtilis based probiotic in two 35-d periods. Period 1 was conducted under thermoneutral conditions (TN; 25.7 ± 1.7 °C) using 150 one-day old chickens (Ross-308) randomly assigned to two dietary treatments: TN birds fed a standard diet without (TN-S) or added with 0.05% probiotic (TN-P). Period 2 was conducted under HS (29.3 ± 2.6 °C) with 120 one-day old chickens fed the standard diet without (HS-S) or with 0.05% probiotic (HS-P). On day 35, ten birds per treatment were sacrificed and jejunum was collected. HS and probiotic supplementation reduced feed intake from d-1 to d-35 (P < 0.01). Feed conversion from day 1 to 21 was better in HS compared to TN chickens, but the opposite occurred from d-1 to d-35 (P < 0.01). Overall, from d-1 to d-35, body weight and daily gain were lower in HS than TN chickens (P < 0.01), but these variables were higher in HS-P than HS-S chickens (P < 0.05). HS chickens reduced villi height, and crypt depth, and increased villi height:crypt depth ratio compared to TN chickens (P < 0.01), but probiotic supplementation increased them (P < 0.01). Probiotic supplementation increased claudin-5 expression during TN and HS periods (P < 0.05), and TJP-1 during TN period (P < 0.05), but decreased occludin expression during HS (P < 0.05). In general, Bacillus subtilis supplementation positively impacts performance and epithelium integrity of the small intestine of HS broiler chickens.</p>","PeriodicalId":23329,"journal":{"name":"Tropical animal health and production","volume":"57 5","pages":"235"},"PeriodicalIF":1.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic parameters estimate of milk yield and composition of Bhadawari buffalo in India.","authors":"Badri Prasad Kushwaha, Sultan Singh, Krishna Kunwar Singh, Deepak Upadhyay, Pooja Tamboli, Tirtha Kumar Datta, Sylvia Lalhmingmawii, Indrajit Gayari, Ajoy Mandal","doi":"10.1007/s11250-025-04491-8","DOIUrl":"https://doi.org/10.1007/s11250-025-04491-8","url":null,"abstract":"<p><p>The objective of this research was to determine the genetic parameters of the test day milk yield and milk constituents (fat, Solid-not-fat, total solid, protein, and lactose) in Bhadawari buffaloes maintained at the ICAR-Indian Grassland and Fodder Research Institute in Jhansi, Uttar Pradesh, India. Data on 2593 lactation records from 93 Bhadawari buffaloes, descended from 16 sires and 43 females were analyzed which covered a period of eight-years (2014 to 2021). In estimating the genetic parameters of all the traits under consideration, the Bayesian approach and Restricted Maximum Likelihood method were employed. The heritability estimates for test day milk yield (MY), fat (F), solid-not-fat (SNF), total solids (TS), protein (P), and lactose percentage of milk ranged between 0.05-0.06, 0.09-0.11, 0.12-0.13, 0.12-0.14, 0.01-0.08 and 0.06-0.07, respectively. For MY (0.05-0.06), F (0.12), SNF (0.08-0.09), TS (0.13), P (0.03-0.18), and lactose (0.06) in both approaches, a low proportion of variations linked to the permanent environmental effect (c² effect) of animals were detected. Repeatability measures for all the traits under study were low to moderate in nature, which ranged from 0.10 to 0.27. The genetic and phenotypic correlations between various traits analyzed exhibited a vast range of magnitudes where a low to high positive genetic correlations were observed for all except between milk yield and fat as well as milk yield and TS. The phenotypic correlations were also positive but low for all the traits under consideration except for milk yield with fat, SNF and TS.</p>","PeriodicalId":23329,"journal":{"name":"Tropical animal health and production","volume":"57 5","pages":"238"},"PeriodicalIF":1.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dietary interaction of sodium bentonite and litter treated with aluminum sulfate on growth performance, litter quality and serum biochemical indices of Nigerian Noiler birds.","authors":"Celestine Ezenwosu, Anselm E Onyimonyi, Charles O Osita, Nnenna W Anizoba, Chekwube M Ugwu, Romanus C Ezeokonkwo","doi":"10.1007/s11250-025-04482-9","DOIUrl":"https://doi.org/10.1007/s11250-025-04482-9","url":null,"abstract":"<p><p>A 6-weeks study was designed to determine the effect of dietary interaction of sodium bentonite and litter treated with aluminum sulfate on growth performance, litter quality and serum biochemical indices of Nigerian Noiler birds. A total of 180 mixed sex (2 weeks old) Nigerian Noiler birds were allotted to six treatment groups in a 3 × 2 factorial arrangements in a completely randomized design with 5 replicates of 6 birds each and were fed ad libitum. Sodium bentonite was used at 3 levels (0, 15 and 30 g/kg), while aluminum sulfate was used at 2 levels (0 and 400 g/3 kg litter). Treatments include: T1 = 0 g NaB /kg + 0 g alum/3 kg litter, T2 = 15 g NaB /kg + 0 g alum /3 kg litter, T3 = 30 g NaB /kg + 0 g alum/3 kg litter, T4 = 0 g NaB /kg + 400 g alum /3 kg litter, T5 = 15 g NaB /kg + 400 g alum/3 kg litter and T6 = 30 g NaB /kg diet + 400 g alum/3 kg litter. Results showed that the interaction of dietary sodium bentonite and litter treated with aluminum sulfate caused significant effect (p < 0.05) on the values of feed intake, ADFI, WG, ADWG, feed cost per kg gain, litter NH₃, moisture, pH, TVC, salmonella, serum cholesterol, LDL and triglyceride, ALT, AST and bilirubin. Birds fed sodium bentonite and on litter treated with aluminum sulfate performed better (p < 0.05) than the control group. Litter moisture and pH were lower (p < 0.05) in treated litter than the control. Litter ammonia gas of 28.66 observed in control litter was higher (p < 0.05) than the 18.50, 17.00, 16.66, 12.50 and 13.65 recorded in T2-T6. TVC value of 0.83 × 10⁷ and salmonella value of 1.53 × 10⁷ obtained in control litter were the highest (p < 0.05) across the treatments. Serum cholesterol, LDL and triglyceride were higher (p < 0.05) in treated groups, while the serum liver enzymes and bilirubin values were also lower (p < 0.05) in treated groups compared to control. In summary, the study observed that dietary sodium bentonite and litter treated with aluminum sulfate improved the litter quality without any damaging impact on the growth performance and serum biochemical parameters of the birds. It was concluded that 15 g NaB /kg + 400 g alum/3 kg litter can be applied by the poultry farmers to enhance the performance, litter quality and health of avian species.</p>","PeriodicalId":23329,"journal":{"name":"Tropical animal health and production","volume":"57 5","pages":"237"},"PeriodicalIF":1.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence of Neospora spp. in horses reared in rural and urban areas in southern Brazil.","authors":"Milena Carol Sbrussi Granella, Rubens Peres Mendes, Mariana da Silva Casa, Gianlucca Simão Nadal Ribeiro, Luis Antônio Sangioni, Fernanda Silveira Flores Vogel, Patrícia Braünig, Paulo Eduardo Ferian, Fabiano Zanini Salbego, David Germano Gonçalves Schwarz, Joandes Henrique Fonteque","doi":"10.1007/s11250-025-04496-3","DOIUrl":"https://doi.org/10.1007/s11250-025-04496-3","url":null,"abstract":"<p><p>Protozoan Neospora spp. is known to negatively affect horses reared in rural and urban environments, being studied for causing abortion, neonatal mortality and central nervous system disorders in horses. This study aimed to verify the occurrence of anti-Neospora spp. antibodies in horses bred in rural and urban areas from southern Brazil and the risk factors associated with infection. The study consisted of 400 horses, which 241 were bred in rural areas and 159 in urban areas. The detection of serum anti-Neospora spp. antibodies was determined using Immunofluorescence Antibody Test (IFAT). The epidemiological questionnaire was applied to owners to determine the factors associated with the infection. The total seropositivity of the animals was 11% (43/400, 95% CI = 7,71-13,78%), distributed into 10% (24/241, 95% CI = 6,18-13,74%) in the rural and 12% (19/159, 95% CI = 6,91 - 16,99%) in the urban group (serum titer ≥ 50). There was no significant difference between rural and urban groups (p = 0.642; OD = 1.31; 95% CI = 0.663-2.58). Stallions and geldings in the rural group were four times more predisposed to presenting anti-Neospora spp. antibodies (p = 0.028; OR = 4.36; 95% CI = 1.164; 14.000). In conclusion, the breeding area is not correlated with seropositivity for Neospora spp. and stallions and geldings raised in rural environments from southern Brazil are more predisposed to infection. There are still poorly elucidated aspects of the infection by Neospora spp. in horses of different sexes, reared in different breeding environments that deserve our concern and require further studies.</p>","PeriodicalId":23329,"journal":{"name":"Tropical animal health and production","volume":"57 5","pages":"236"},"PeriodicalIF":1.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential benefits of Ginkgo biloba in poultry nutrition: an updated review.","authors":"Ayman H Abd El-Aziz, Daniel Mota-Rojas, Bukola Christiana Majekodunmi, Monsuru Oladimeji Abioja","doi":"10.1007/s11250-025-04487-4","DOIUrl":"https://doi.org/10.1007/s11250-025-04487-4","url":null,"abstract":"<p><p>The need for more efficient poultry production to meet the increasing demand for animal protein due to the increase in global human population informs the use of antibiotic growth promoters (AGPs). Rising worries about the possible development of antibiotic-resistant bacteria and residues in chicken products make it imperative to develop alternatives to AGP. Such alternatives must possess qualities that are safe for public health, cost-efficient, environmentally-friendly,possessing antibacterial properties and ensure better growth performance in birds. More and more people are starting to see the value of functional food additives. Therefore, searching for novel feed items with useful characteristics is a primary focus of present-day research. Ginkgo biloba (GB) tree is of such promising materials in nature. It is a sole member of the Ginkgoaceae family, which is a subclass of Ginkgoatae. The leaves of GB contain flavonoids, terpenoids, polyphenols, polysaccharides, vitamins and minerals. There is current evidence that extracts from Ginkgo biloba plant exhibits antioxidant, antibacterial, antiplatelet, anticancer, neuro-protective and cardio-protective effects. These content have led to its growing popularity as a functional food ingredient in recent years. Evidence of its effectiveness is found in various literatures. However, there have not been a synthesized detailed summary of the results scattered in literature. Hence, this review aims to update the knowledge about the potential effects of GB supplementation in broiler chickens on growth performance, blood biochemistry, immunological function, carcass and meat quality, intestinal morphology, economic feasibility, and the expression of specific genes related to growth and antioxidant processes. Future considerations on the utilization of GB leaves and extracts in commercial feed of chickens are also presented.</p>","PeriodicalId":23329,"journal":{"name":"Tropical animal health and production","volume":"57 5","pages":"233"},"PeriodicalIF":1.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gastrointestinal development and tight junctions of preweaned calves supplemented with sodium butyrate in the starter mixture and whole milk.","authors":"Zhanhe Zhang, Donglin Wu, Xintong Li, Yang Jia, Jingwei Qi, David L Harmon, Ming Xu","doi":"10.1007/s11250-025-04464-x","DOIUrl":"https://doi.org/10.1007/s11250-025-04464-x","url":null,"abstract":"<p><p>The effects of sodium butyrate (SB) supplementation in whole milk (WM), and/or a starter mixture (SM) on the growth performance, selected blood parameters, gastrointestinal tract (GIT) development, and tight junction (TJ) proteins of the gastrointestinal tract in preweaned calves were determined. Eighty calves, averaging of 3 (± 0.74) d old, were randomly allocated into 1 of 4 groups (n = 20 per group) and were fed (1) WM and SM without SB (control group); (2) SM supplemented with SB (0.3% of dry matter basis; SM group); (3) WM supplemented with SB (WM, 8.8 g/d) (WM group); or (4) WM and SM supplemented with SB (SMWM group). At d 74, four calves from each group were euthanized for in-depth analysis. The addition of SB to the SM increased the ADG and reduced fecal scores throughout the preweaned period (P < 0.05). SB supplementation in WM notably lowered diarrhea incidence throughout the preweaned period (P < 0.05). The addition of SB to the SM increased blood beta-hydroxybutyric acid and diamine oxidase concentrations at d 24 and insulin-like growth factor 1 concentrations at d 44 (P < 0.05). SB addition to WM prominently enhanced rumen papilla dimensions but reduced jejunum crypt depth alongside villus height and depth (P < 0.05). The addition of SB to the SM diminished the mucous layer thickness of the rumen and increased TJ protein expression in the GIT (P < 0.05). Based on these results, we found that the addition of SB to the SM and WM had a positive effect on calf general health, blood indexes, and GIT development. The addition of SB to both WM and SM could be recommended for preweaned calves.</p>","PeriodicalId":23329,"journal":{"name":"Tropical animal health and production","volume":"57 5","pages":"234"},"PeriodicalIF":1.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Do foraging hotspots defined by key browse species facilitate interaction between livestock and wildlife?","authors":"Clarice Princess Mudzengi, Amon Murwira, Fadzai Zengeya, Tinyiko Halimani, Herve Fritz, Chrispen Murungweni","doi":"10.1007/s11250-025-04484-7","DOIUrl":"https://doi.org/10.1007/s11250-025-04484-7","url":null,"abstract":"<p><p>Livestock based rural livelihoods in close proximity to conservation areas are often associated with human-wildlife conflict. A deeper comprehension of factors contributing to this can help develop strategies to resolve the impact of human-wildlife conflict on livestock production. We tested whether dry season livestock foraging hotspots were linked to key browse species (KBS), and whether they facilitated livestock-wildlife interactions. We detected livestock browsing hotspots, determined their patch characteristics, and established factors influencing their occurrence. We used kernel density estimation (KDE) and nearest neighbor hierarchical clustering (Nnh). The KDE is a non-parametric method that uses a density estimation technique, while the Nnh is a method used to group similar data points based on their distances. Both methods detected three hotspots comprising Colophospermum mopane and Dichrostachys cinerea mixed woodland, and D. cinerea vegetation. Spatial overlap between detected hotspots was relatively high (Szymkiewicz-Simpson coefficient (Overlap Coefficient) = 0.7; Minta Index = 0.6). Fine-scale habitat patch quantifications indicated that connectivity between D. cinerea patches was higher than D. cinerea and C. mopane patches (Connectivity Index = 60.0%; Patch Cohesion Index = 98.0%). Distance from water was the most significant factor (P < 0.05; AUC = 0.9; AICc = 59.1), explaining probability of occurrence of hotspots. Multiple-distance scale spatial cluster analysis showed significant spatial associations between elephants and livestock. Results suggest that KBS are concentrated around browsing hotspots, which could facilitate livestock-wildlife interactions. Improving livestock feed availability outside conservation areas, and spatial planning for grazing management and wildlife corridors could mitigate human-wildlife conflict. Overall, this study provides valuable insights into foraging behaviours at the livestock-wildlife interface, and underscores the need for sustainable management strategies to balance conservation and rural livelihoods, thereby improving coexistence.</p>","PeriodicalId":23329,"journal":{"name":"Tropical animal health and production","volume":"57 5","pages":"232"},"PeriodicalIF":1.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guava leaf extract as a catalyst for enhanced rabbit health and performance in sub-tropical regions: an updated review.","authors":"A H Abd El-Aziz","doi":"10.1007/s11250-025-04485-6","DOIUrl":"10.1007/s11250-025-04485-6","url":null,"abstract":"<p><p>For sustainable intensification of rabbit industry, it is imperative to explore the potential of non-conventional feed resources. The researchers were driven to discover solutions for the shortage of feed in livestock production because of the ongoing rise in costs associated with conventional feed resources. Their efforts were directed at enhancing conventional sources and investigating alternative feed sources, including agricultural and agro-based industrial by-products. Therefore, the review examines the possible advantages of using guava leaf extract (Psidium guajava) as an example of non-conventional feed additive to improve the growth performance and overall health of commercially raised rabbits. Guava leaf extract contains a high concentration of bioactive substances, such as antioxidants, vitamins, and vital minerals. These substances have demonstrated advantageous effects on the feed efficiency, growth rate, and overall health of rabbits. This review article provides a thorough analysis of the current understanding and updates the knowledge about the physiological effects of adding guava leaf extract to rabbit diets, with a specific emphasis on its economic benefits. The findings demonstrated notable enhancements in feed conversion efficiency, growth rate, and immune system functionality, coupled with reductions in the total lipids levels and pathogenic microorganisms. Potential avenues for future investigation encompass the refinement of dosage, examination of synergistic impacts with other dietary constituents, and implementation of extended studies to validate the enduring advantages.</p>","PeriodicalId":23329,"journal":{"name":"Tropical animal health and production","volume":"57 5","pages":"231"},"PeriodicalIF":1.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12119758/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144161107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An automatic approach for the classification of lumpy skin disease in cattle.","authors":"Fakhre Alam, Asad Ullah, Mohammed A Rohaim, Muhammad Munir, Aftab Hussain","doi":"10.1007/s11250-025-04475-8","DOIUrl":"https://doi.org/10.1007/s11250-025-04475-8","url":null,"abstract":"<p><p>Lumpy Skin Disease (LSD) presents significant risks and economic challenges to global cattle farming. Effective and accurate classification of LSD is essential for managing the disease and reducing its impacts. Manual diagnosis is time-consuming, labor-intensive, and requires experienced personnel. Automated classification methods provide advantages by reducing labor and improving accuracy. This study proposes an automated algorithm for LSD classification using machine learning. The method uses a carefully curated dataset of images from both LSD-infected cattle and healthy cattle. Inception V3 was employed to extract features from complex lesion patterns in infected cattle images, comparing them to healthy cattle images. Support Vector Machines (SVM) were used to classify the extracted features. The results show the model achieved an 84% accuracy rate, with precision at 80%, recall at 83%, and an F1 score of 82%. These results were compared with other machine learning models, including Logistic Regression, Random Forest, Decision Tree, and AdaBoost. SVM outperformed other models, demonstrating consistent evaluation precision at 0.84. For further enhancement, expanding the dataset with high-quality images and applying advanced machine learning algorithms like Vision Transformers (ViTs), MobileNetV2, and Visual Geometry Group (VGG) could refine automated LSD classification. The aim is to improve disease management practices in the livestock industry through better classification systems.</p>","PeriodicalId":23329,"journal":{"name":"Tropical animal health and production","volume":"57 5","pages":"230"},"PeriodicalIF":1.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144161105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of different ratio of sweet sorghum silage to corn silage diets on serum metabolome of lactating dairy cows.","authors":"Zhenping Hou, Xia Yu, Duanqin Wu","doi":"10.1007/s11250-025-04483-8","DOIUrl":"https://doi.org/10.1007/s11250-025-04483-8","url":null,"abstract":"<p><p>The objective of this study was to investigate the impact of partially substituting whole corn silage (CS) with sweet sorghum silage (SS) on serum metabolites in dairy cows employing by Liquid chromatography-mass spectrometry (LC-MS). Thirty mid-lactation Holstein dairy cows (112 ± 24.04 days in milk) were divided into the control group (CON) received 40% CS and 0% SS, Group1 received 30% CS and 10% SS, while Group2 received 20% CS and 20% SS, respectively. Serum samples were collected on day 45 of the formal experiment and subjected to LC-MS analysis to detect metabolites. The results revealed that 29 different metabolites were obtained in both CON and Group1; however, 22 metabolites in Group1 showed up-regulation compared to those in CON including N-Methylhydantoin, Betaine, Isoleucine, etc. The levels of 7 metabolites, including L-Kynurenine, Nandrolone, and Testosterone propionate, were found to be down-regulated. Sixteen metabolites were detected in the serum of Group2 and CON dairy cows. Compared with CON, Group2 exhibited up-regulation of 13 metabolites such as Benzaldehyde, Linoleic acid, Phe-Glu. Conversely, three metabolites including Triglycerides (TG), Uric acid and 5-Hydroxyindole-3-acetic acid showed down-regulation. A total of 20 differential metabolites were identified in Group1 and Group2. Among these, 14 metabolites such as 2-Ethylacrylic acid, Succinic acid, and Glutamic acid were up-regulated while 6 metabolites like Pyridoxine, Phosphocholine, and Quinidine showed down-regulation. These differentially expressed metabolites are primarily associated with various pathways including Aminoacyl-tRNA, Arginine, Phenylalanine and tryptophan biosynthesis, Citrate cycle etc. In conclusion, substituting up to 50% of CS with SS had no negative effects on serum metabolites, indicating that SS can partially replace CS in the diets of high-producing lactating dairy cows without adding extra grain, when diets are fed for a short time under our feeding experimental conditions.</p>","PeriodicalId":23329,"journal":{"name":"Tropical animal health and production","volume":"57 5","pages":"229"},"PeriodicalIF":1.7,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}