Translational Research最新文献

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IF 6.4 2区医学

Translational Research Pub Date : 2025-05-28 DOI: 10.1016/S1931-5244(25)00060-X

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IF 6.4 2区医学

Translational Research Pub Date : 2025-05-28 DOI: 10.1016/S1931-5244(25)00059-3

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Editorial Advisory Board 编辑顾问委员会

IF 6.4 2区医学

Translational Research Pub Date : 2025-05-28 DOI: 10.1016/S1931-5244(25)00058-1

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sFRP5 ameliorates atherosclerosis by suppressing the JNK/TLR9 pathway in macrophages sFRP5通过抑制巨噬细胞的JNK/TLR9通路改善动脉粥样硬化。

IF 6.4 2区医学

Translational Research Pub Date : 2025-05-22 DOI: 10.1016/j.trsl.2025.05.004

Yue Yu , Han Chen , Rui Wang , Fei Xu, Jiasheng Yin, Tongtong Zang, Changyi Zhou, Chengpeng Liu, Chaofu Li, Li Shen, Junbo Ge

{"title":"sFRP5 ameliorates atherosclerosis by suppressing the JNK/TLR9 pathway in macrophages","authors":"Yue Yu , Han Chen , Rui Wang , Fei Xu, Jiasheng Yin, Tongtong Zang, Changyi Zhou, Chengpeng Liu, Chaofu Li, Li Shen, Junbo Ge","doi":"10.1016/j.trsl.2025.05.004","DOIUrl":"10.1016/j.trsl.2025.05.004","url":null,"abstract":"<div><div>Secreted frizzled related protein 5 (sFRP5), an anti-inflammatory adipokine, plays a crucial role in various diseases, and its serum levels are low in patients with coronary artery disease (CAD). However, its role in atherosclerosis remains unclear. Therefore, we investigated the correlation between sFRP5 and plaque stability, along with the molecular mechanisms underlying atherosclerosis. In patients with CAD, serum sFRP5 levels were positively correlated with plaque stability, a predictor of thin-cap fibroatheromas (TCFAs). Recombinant sFRP5 (r-sFRP5) supplementation significantly increased plaque stability and ameliorated atherosclerosis progression in <em>ApoE<sup>-/-</sup></em> mice. Aortic RNA-sequencing (RNA-seq) revealed sFRP5-mediated regulation in inflammatory cells. Our experiments confirmed that sFRP5 inhibits inflammation and macrophage migration. Mechanistically, Toll-like receptor 9 (TLR9) was identified as a downstream target of sFRP5, and sFRP5 suppressed TLR9 expression by decreasing c-Jun N-terminal kinase (JNK) phosphorylation. These findings suggest that serum sFRP5 levels are associated with plaque stability and play a protective role in atherosclerosis by attenuating inflammation and macrophage infiltration via inhibition of the JNK/TLR9 pathway, thereby ameliorating the progression of atherosclerosis. This study highlights the potential of sFRP5 as both a biomarker and therapeutic target for plaque stability in atherosclerosis.</div></div>","PeriodicalId":23226,"journal":{"name":"Translational Research","volume":"281 ","pages":"Pages 1-13"},"PeriodicalIF":6.4,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144133401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Modeling diabetic epitheliopathy using 3D-Organotypic corneal epithelium 利用3d器官型角膜上皮建立糖尿病上皮病变模型。

IF 6.4 2区医学

Translational Research Pub Date : 2025-05-17 DOI: 10.1016/j.trsl.2025.05.003

Grazia Maugeri , Agata Grazia D’Amico , Salvatore Saccone , Francesca Bruno , Elisabetta Pricoco , Davide Scollo , Teresio Avitabile , Antonio Longo , Velia D’Agata

{"title":"Modeling diabetic epitheliopathy using 3D-Organotypic corneal epithelium","authors":"Grazia Maugeri , Agata Grazia D’Amico , Salvatore Saccone , Francesca Bruno , Elisabetta Pricoco , Davide Scollo , Teresio Avitabile , Antonio Longo , Velia D’Agata","doi":"10.1016/j.trsl.2025.05.003","DOIUrl":"10.1016/j.trsl.2025.05.003","url":null,"abstract":"<div><div>Diabetic keratopathy (DK) is a degenerative corneal disease occurring in more than 50 % of diabetic patients. DK is correlated with the hyperglycemic state causing morphological and functional changes in corneal layers. Currently, most studies on the cornea are performed on two-dimensional (2D) cultures <em>in vitro</em> or animal models. Although 2D culture models can provide large amounts of data at low cost, they poorly represent the complex pathophysiology of the human cornea and hardly predict <em>in vivo</em> responses that can be achieved with animal model studies. However, the use of the latter presents ethical problems. Therefore, it is necessary to identify new strategies and models that can integrate the information validly and effectively, to reduce the number of animals used. Here, we used human corneal epithelial cells (hCECs) derived from donor cornea differentiated into three-dimensional (3D)-organotypic air-liquid interface (ALI), which resemble the features of the corneal epithelium. The 3D-organotypic ALI corneal epithelium was subjected to high-glucose conditions to generate a model of diabetic epitheliopathy. Our model showed well-established molecular and cellular characteristics of this pathology, such as epithelial defects and inflammation, with increased expression of IL-1β, TNF-<span><math><mi>α</mi></math></span>, p-NF-kB, COX-2, MMP-2 and MMP-9. The data provided highlight the utility of 3D-organotypic corneal epithelium in modeling diabetic epitheliopathy, offering new avenues in drug screening, as well as in precision and personalized medicine.</div></div>","PeriodicalId":23226,"journal":{"name":"Translational Research","volume":"280 ","pages":"Pages 55-63"},"PeriodicalIF":6.4,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144103120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Abundance of dopamine and its receptors in the brain and adipose tissue following diet-induced obesity or caloric restriction 饮食引起的肥胖或热量限制后大脑和脂肪组织中多巴胺及其受体的丰度

IF 6.4 2区医学

Translational Research Pub Date : 2025-05-08 DOI: 10.1016/j.trsl.2025.05.001

Fleur W Hukema , Susanne Hetty , Christakis Kagios , Sofia Zelleroth , Giovanni Fanni , Maria J Pereira , Maria K Svensson , Magnus Sundbom , Anna Nilsson , Per E Andrén , Erika Roman , Jan W Eriksson

{"title":"Abundance of dopamine and its receptors in the brain and adipose tissue following diet-induced obesity or caloric restriction","authors":"Fleur W Hukema , Susanne Hetty , Christakis Kagios , Sofia Zelleroth , Giovanni Fanni , Maria J Pereira , Maria K Svensson , Magnus Sundbom , Anna Nilsson , Per E Andrén , Erika Roman , Jan W Eriksson","doi":"10.1016/j.trsl.2025.05.001","DOIUrl":"10.1016/j.trsl.2025.05.001","url":null,"abstract":"<div><div>While obesity and type 2 diabetes (T2D) are associated with altered dopaminergic activity in the central nervous system and in adipose tissue (AT), the directions and underlying mechanisms remain inconclusive. Therefore, we characterized changes in the abundance of dopamine, its metabolites, and receptors DRD1 and DRD2 in the brain and AT upon dietary intervention or obesity. Male Wistar rats were fed either a standard pellet diet, a cafeteria diet inducing obesity and insulin resistance, or a calorie-restricted diet for 12 weeks. Abundance of dopamine and its receptors DRD1 and DRD2 were examined in brain regions relevant for feeding behavior and energy homeostasis. Furthermore, DRD1 and DRD2 protein levels were analyzed in rat inguinal and epidydimal AT and in human subcutaneous and omental AT from individuals with or without obesity. Rats with diet-induced obesity displayed higher dopamine levels, as well as DRD1 or DRD2 receptor levels in the caudate putamen and the nucleus accumbens core. Surprisingly, caloric restriction induced similar changes in DRD1 and DRD2, but not in dopamine levels, in the brain. Both diets reduced DRD1 abundance in inguinal and epidydimal AT, but upregulated DRD2 levels in inguinal AT. Furthermore, in human obesity, DRD1 protein levels were elevated only in omental AT, while DRD2 was upregulated in both omental and subcutaneous AT. These findings highlight dopaminergic responses to changes in energy balance, occurring both in the brain and AT. We propose that dopaminergic pathways are involved in tissue crosstalk during the development of obesity and T2D.</div></div>","PeriodicalId":23226,"journal":{"name":"Translational Research","volume":"280 ","pages":"Pages 41-54"},"PeriodicalIF":6.4,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Information for Readers 读者资讯

IF 6.4 2区医学

Translational Research Pub Date : 2025-05-01 DOI: 10.1016/S1931-5244(25)00045-3

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Editorial Advisory Board 编辑顾问委员会

IF 6.4 2区医学

Translational Research Pub Date : 2025-05-01 DOI: 10.1016/S1931-5244(25)00043-X

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Author Guidelines 作者指导方针

IF 6.4 2区医学

Translational Research Pub Date : 2025-05-01 DOI: 10.1016/S1931-5244(25)00044-1

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Cardioprotective effects of PARP Inhibitors: A meta-analysis of animal studies PARP抑制剂的心脏保护作用：动物研究的荟萃分析

IF 6.4 2区医学

Translational Research Pub Date : 2025-04-30 DOI: 10.1016/j.trsl.2025.04.004

Seong Kyung Kim , Jae Hyun Kim , Inyeong Moon , Jiwon Min , Jieun Park , Myeong Gyu Kim

{"title":"Cardioprotective effects of PARP Inhibitors: A meta-analysis of animal studies","authors":"Seong Kyung Kim , Jae Hyun Kim , Inyeong Moon , Jiwon Min , Jieun Park , Myeong Gyu Kim","doi":"10.1016/j.trsl.2025.04.004","DOIUrl":"10.1016/j.trsl.2025.04.004","url":null,"abstract":"<div><div>Poly(adenosine diphosphate [ADP] ribose) polymerase (PARP) inhibitors are expected to provide benefits to the cardiovascular system. However, the cardioprotective effect of PARP inhibitors has not been systematically reviewed or quantitatively analyzed. This study aimed to assess the cardioprotective effects of PARP inhibitors through a meta-analysis of animal studies. Three databases PubMed, Web of Sciences, and Embase were searched until September 1, 2023. The risk of bias was assessed using SYRCLE’s Risk of Bias. A total of 74 animal studies that investigated the cardiac function of PARP inhibitors compared to placebo or vehicle, were included. Outcome measures were hemodynamic indexes, cardiac contractility, and biomarkers of myocardial injury. Pooled effect size was estimated using a random-effects model with RevMan 5.4. PARP inhibitors were associated with enhanced hemodynamic indexes, including cardiac output (standardized mean difference, 0.86 [95 % CI, 0.54 to 1.17]; <em>p</em> < 0.00001) and stroke volume (0.42 [0.07 to 0.76]; <em>p</em> = 0.02). PARP inhibitors were associated with increased cardiac contractility, including ejection fraction (0.71 [0.42 to 1.01]; <em>p</em> < 0.00001) and fractional shortening (0.96 [0.62 to 1.31]; <em>p</em> < 0.00001). PARP inhibitors were associated with decreased troponin І (-1.42 [-2.16 to -0.68]; <em>p</em> = 0.0002), plasma B-type natriuretic peptide (-0.95 [-1.56 to -0.33]; <em>p</em> = 0.003), creatine kinase (-1.81 [-2.63 to -0.99]; <em>p</em> < 0.0001), and infarct size (-1.58 [-2.01 to -1.14]; <em>p</em> < 0.00001). PARP inhibitors improve cardiac functions and attenuate myocardial injury in animals, which indicate the cardioprotective effects. Further human studies are necessary.</div></div>","PeriodicalId":23226,"journal":{"name":"Translational Research","volume":"280 ","pages":"Pages 29-40"},"PeriodicalIF":6.4,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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