IF 2.8 3区生物学

Traffic Pub Date : 2026-06-01 DOI: 10.1111/tra.70035

Paul Rosas-Santiago, Jorge Luis Ruiz Salas, Elizabeth Cordoba, Francisco Vera-López Portillo, Prisciluis Caheri Salas-Navarrete, Jaime Arturo Pimentel Cabrera, Alfredo Martínez, Nayeli Imelda Roldan Ramírez, Dieter Chavelas Hernández, Andrés Saralegui-Amaro, Martha Calahorra, Hana Sychrová, Olga Zimmermannová

{"title":"The Lack of a COPII Cargo Receptor Erv14 Impacts Physiological Functions of the Vacuole in Saccharomyces cerevisiae.","authors":"Paul Rosas-Santiago, Jorge Luis Ruiz Salas, Elizabeth Cordoba, Francisco Vera-López Portillo, Prisciluis Caheri Salas-Navarrete, Jaime Arturo Pimentel Cabrera, Alfredo Martínez, Nayeli Imelda Roldan Ramírez, Dieter Chavelas Hernández, Andrés Saralegui-Amaro, Martha Calahorra, Hana Sychrová, Olga Zimmermannová","doi":"10.1111/tra.70035","DOIUrl":"10.1111/tra.70035","url":null,"abstract":"Erv14 is a cargo receptor of COPII vesicles, which is necessary for the efficient trafficking of various membrane proteins. In this work, we demonstrate that the deletion of the ERV14 gene impacts various physiological functions of the vacuole. Compared to the wild-type cells, cells lacking the ERV14 gene exhibited higher vacuolar pH and altered vacuolar morphology with increased fragmentation. In addition, erv14Δ cells exhibit a thinner cell wall and an impaired process of endocytosis. We also found the importance of ERV14 for cells to overcome environmental stresses, such as neutral external pH, increased zinc and calcium concentrations, and high temperature. Furthermore, comparing gene expression, proteome analysis, and structural modeling revealed new interactions between Erv14 and several vacuolar proteins, including subunits of V-ATPase and other proteins involved in carbon metabolism.","PeriodicalId":23207,"journal":{"name":"Traffic","volume":"27 2","pages":"e70035"},"PeriodicalIF":2.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13106738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147782069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling Lysosomal Exocytosis: From Molecular Mechanisms to Physiological Functions. 解溶酶体胞吐：从分子机制到生理功能。

IF 2.5 3区生物学

Traffic Pub Date : 2026-03-01 DOI: 10.1111/tra.70026

Shanshan Jiang, Jingyi Fu, Shan Li, Zehui Li, Qirui Zhao, Yuqi Yin, Mei Yang, Yonghua Wang

{"title":"Unraveling Lysosomal Exocytosis: From Molecular Mechanisms to Physiological Functions.","authors":"Shanshan Jiang, Jingyi Fu, Shan Li, Zehui Li, Qirui Zhao, Yuqi Yin, Mei Yang, Yonghua Wang","doi":"10.1111/tra.70026","DOIUrl":"10.1111/tra.70026","url":null,"abstract":"Lysosomal exocytosis is a fundamental cellular process that involves the fusion of lysosomes with the plasma membrane and the release of lysosomal contents into the extracellular space. This review provides an in-depth analysis of the molecular mechanisms, physiological functions, and disease implications of lysosomal exocytosis, highlighting recent advances and novel aspects. We discuss the intricate molecular machinery that orchestrates lysosomal trafficking, docking, and fusion, as well as the critical roles of lysosomal exocytosis in maintaining cellular homeostasis, facilitating intercellular communication, and contributing to specialized cellular functions. Additionally, the review explores the complex involvement of lysosomal exocytosis in various disease states, including lysosomal storage disorders, neurodegenerative diseases, cancers, and immune system disorders, underlining its potential as a therapeutic target. By identifying current knowledge gaps and providing future research directions, this review aims to stimulate further investigation into the multifaceted nature of lysosomal exocytosis and its implications for human health and disease.","PeriodicalId":23207,"journal":{"name":"Traffic","volume":"27 1","pages":"e70026"},"PeriodicalIF":2.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12744940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145850630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein Kinase D Regulates Kinesin-Mediated VEGFR2 Trafficking and Influences Retinal Endothelial Permeability. 蛋白激酶D调节运动蛋白介导的VEGFR2运输并影响视网膜内皮通透性。

IF 2.8 3区生物学

Traffic Pub Date : 2026-03-01 DOI: 10.1111/tra.70032

Benedict Ryungrae Kim, Hyun-Dong Cho, Yudai Kudo, Hirushi Gunasekara, Geyi Li, Kayeman Tu, Stephen B Waters, Ying S Hu, Kaori H Yamada

{"title":"Protein Kinase D Regulates Kinesin-Mediated VEGFR2 Trafficking and Influences Retinal Endothelial Permeability.","authors":"Benedict Ryungrae Kim, Hyun-Dong Cho, Yudai Kudo, Hirushi Gunasekara, Geyi Li, Kayeman Tu, Stephen B Waters, Ying S Hu, Kaori H Yamada","doi":"10.1111/tra.70032","DOIUrl":"10.1111/tra.70032","url":null,"abstract":"Vascular endothelial growth factor (VEGF) induces angiogenesis and vascular leakage by binding to cell surface receptor VEGFR2. KIF13B, a kinesin 3 family cellular motor, regulates VEGF/VEGFR2 signaling by bringing VEGFR2 to the cell surface. Regulating the trafficking of VEGFR2 could be a potential therapeutic target in pathogenesis originating from abnormal angiogenesis. However, the regulation mechanism of VEGFR2 trafficking is not fully understood. Here, we show that protein kinase D (PKD) phosphorylates KIF13B. The phosphorylation of KIF13B by PKD is required for KIF13B-VEGFR2 interaction and KIF13B-microtubule interaction. Live cell imaging using VEGFR2-mCherry revealed that the PKD inhibitor reduced the mean speed and total distance of VEGFR2 vesicular trafficking. Finally, inhibition of PKD also mitigated VEGF-induced permeability measured by trans-endothelial electrical resistance (TEER). Our results demonstrate how VEGFR2 trafficking is regulated by the phosphorylation of kinesin motor KIF13B by protein kinase PKD. We anticipate that elucidating the underlying mechanism of VEGFR2 trafficking can help progress the therapy development of the target diseases related to abnormal angiogenesis.","PeriodicalId":23207,"journal":{"name":"Traffic","volume":"27 1","pages":"e70032"},"PeriodicalIF":2.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12988601/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146182458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Palmitoyl Acyltransferases Control the Membrane Localization of DNAJC5 to Regulate Unconventional Protein Secretion. 棕榈酰转移酶控制DNAJC5的膜定位调控非常规蛋白分泌。

IF 2.8 3区生物学

Traffic Pub Date : 2026-03-01 DOI: 10.1111/tra.70031

Yue Xu, Robbins Puthenveetil, Juhyung Lee, Layla Saidi, Anirban Banerjee, Yihong Ye

引用次数: 0

Clathrins Are Involved in the Endocytosis of Host Cytosol in the Malaria Parasite. 网格蛋白参与疟原虫宿主胞浆内吞作用。

IF 2.8 3区生物学

Traffic Pub Date : 2026-03-01 DOI: 10.1111/tra.70025

Jun Miao, Amuza Byaruhanga Lucky, Gang Ning, Xian Xia, Xiaoying Liang, Faiza Siddiqui, Hui Min, Chengqi Wang, Xiaolian Li, Z Hong Zhou, Liwang Cui

{"title":"Clathrins Are Involved in the Endocytosis of Host Cytosol in the Malaria Parasite.","authors":"Jun Miao, Amuza Byaruhanga Lucky, Gang Ning, Xian Xia, Xiaoying Liang, Faiza Siddiqui, Hui Min, Chengqi Wang, Xiaolian Li, Z Hong Zhou, Liwang Cui","doi":"10.1111/tra.70025","DOIUrl":"10.1111/tra.70025","url":null,"abstract":"In eukaryotic cells, clathrins interact with the adaptor protein (AP) complex-2 (AP-2) to facilitate endocytosis and AP-1 to mediate secretion and trafficking between the endosome and Golgi. In Plasmodium falciparum, recent studies revealed that the Kelch domain-containing protein 13 and AP-2 participate in hemoglobin uptake via cytostomes. However, clathrins appear not to be involved in this process because they primarily associate with AP-1. To investigate the roles of clathrins in P. falciparum, we characterized the clathrin heavy chain (PfCHC), the clathrin light chain (PfCLC), and the AP-1 γ subunit (PfAP-1 γ). Extensive interactome analyses confirmed the major association of clathrins with AP-1 components alongside proteins involved in cytostome formation. Live-cell imaging and protein colocalization studies showed that PfCHC, PfCLC, and PfAP-1 γ are localized in the parasite cytoplasm, predominantly at the parasite periphery and near the cis-Golgi. Ultrastructural studies using ascorbate peroxidase 2-based electron microscopy confirmed their presence at coated vesicle-like structures at the parasite periphery and, unexpectedly, at the collars of cytostomes. Knockdown of PfCHC led to the formation of abnormally long cytostome tubes and impaired hemoglobin uptake, suggesting that clathrins are involved in cytostome processes in P. falciparum.","PeriodicalId":23207,"journal":{"name":"Traffic","volume":"27 1","pages":"e70025"},"PeriodicalIF":2.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12742282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145834783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Virion Associated Importin Subunit Beta-1 Augments Nuclear Import of HIV-1 Pre-Integration Complex. 病毒粒子相关输入蛋白亚基β -1增强HIV-1预整合复合体的核输入。

IF 2.5 3区生物学

Traffic Pub Date : 2025-10-01 DOI: 10.1111/tra.70021

Sriram Yandrapally, Satarupa Sarkar, Venkateshwarlu Naik, Carsten Münk, Srikanth Rapole, Sharmistha Banerjee

{"title":"Virion Associated Importin Subunit Beta-1 Augments Nuclear Import of HIV-1 Pre-Integration Complex.","authors":"Sriram Yandrapally, Satarupa Sarkar, Venkateshwarlu Naik, Carsten Münk, Srikanth Rapole, Sharmistha Banerjee","doi":"10.1111/tra.70021","DOIUrl":"10.1111/tra.70021","url":null,"abstract":"Import of proviral genome from the cytoplasm to the nucleus is a decisive step in the HIV-1 infection cycle, regulating which can decide the fate of HIV-1 infectivity. Exploring the heterogeneity in the replication potential of viruses emerging from various producer cells, we compared the infectivity dynamics of viruses produced by CD4+ T lymphocytes, a cell type that supports the HIV-1 propagation, with those from astrocytes that allow for limited viral replication. We found the viruses emerging from these two cell types not only differed in their infectivity, but also in the host proteins that get associated with these virions. We focused on Importin β1, an autonomous nuclear transport receptor, which was present in the virion fractions from CD4+ T lymphocytes but absent in those from astrocytes. Our analysis revealed that Importin β1 gets associated with the virus through interactions with HIV-1 Gag and Capsid proteins. Using Importin β1 knockout cell models, we found that virion-associated Importin β1 enhanced viral infectivity by facilitating the import of the viral Pre-integration complex (PIC) into the nucleus of an infected cell. Linking positive factors, such as Importin β1, to emerging virions can determine the viral infectivity in subsequent infection rounds, influencing disease progression.","PeriodicalId":23207,"journal":{"name":"Traffic","volume":"26 10-12","pages":"e70021"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145347520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Copper Homeostasis at the Crossroads of Cellular Metabolism, Epigenetic Regulation and Protein Trafficking. 细胞代谢、表观遗传调控和蛋白质运输十字路口的铜稳态。

IF 2.5 3区生物学

Traffic Pub Date : 2025-10-01 DOI: 10.1111/tra.70024

Vandana Anjana, Smriti Anand, Prateeksha Thakur, Rajshree Pal, Santoshi Acharjee, Ranu Singh, Sugandh Sharma, Manoj Kumawat, Raghuvir Singh Tomar

{"title":"Copper Homeostasis at the Crossroads of Cellular Metabolism, Epigenetic Regulation and Protein Trafficking.","authors":"Vandana Anjana, Smriti Anand, Prateeksha Thakur, Rajshree Pal, Santoshi Acharjee, Ranu Singh, Sugandh Sharma, Manoj Kumawat, Raghuvir Singh Tomar","doi":"10.1111/tra.70024","DOIUrl":"10.1111/tra.70024","url":null,"abstract":"Copper is one of the essential micronutrients utilized as a cofactor in a wide variety of biochemical reactions of metabolic pathways, including mitochondrial respiration and innate immune response. Cellular concentration and distribution of copper is regulated by copper-specific transporters, chaperones, metallothionein proteins and amino acids. Transcription of a major copper metallothionein, CUP1 is epigenetically regulated in Saccharomyces cerevisiae. Mutations in histones dysregulate cellular copper homeostasis due to abnormal epigenetic changes and cause diseases in humans, such as cancerous growth and neurological disorders. Low or higher cellular concentration of copper is associated with disorders such as Menkes and Wilson's disease, respectively. Higher concentrations of copper cause caspase-independent cell death known as cuproptosis and haemolytic anemia. We highlighted the existing knowledge regarding the significance of epigenetics and cellular factors in the regulation of copper metabolism and copper-regulated protein trafficking. We have also proposed a few future directions to explore the role of cellular pH dynamics, stoichiometry among metals, amino acids and protein metabolism, histone modifications, autophagy and mitochondrial respiration in regulating cellular copper metabolism. Altogether, we provide a comprehensive summary of cellular factors targeting copper metabolism for dissecting the underlying complex mechanism of copper dynamics in normal physiology and diseases.","PeriodicalId":23207,"journal":{"name":"Traffic","volume":"26 10-12","pages":"e70024"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145649452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond the Secretory Pathway: New Insights Into Protein Release. 超越分泌途径：对蛋白质释放的新见解。

IF 2.8 3区生物学

Traffic Pub Date : 2025-10-01 DOI: 10.1111/tra.70022

Ruey-Hwa Chen, Antonio J Costa-Filho, Jayanta Debnath, Thierry Galli, Liang Ge, Deborah Goberdhan, Wei Guo, Kangmin He, Ralf Jacob, Tiebang Kang, Min Goo Lee, Lian Li, Fabio Lolicato, Jun Lu, Vivek Malhotra, Walter Nickel, Vassiliki Nikoletopoulou, Stacey K Ogden, Georgia Maria Sagia, Feng Shao, Anbing Shi, Clotilde Thery, Christel Vérollet, Julien Villeneuve, Frederik Verweij, Yanzhuang Wang, Juan Wang, Shenjie Wu, Yihong Ye, Hang Yin, Li Yu, Min Zhang, Ying Zhang, Xin Zhou, Chiara Zurzolo

{"title":"Beyond the Secretory Pathway: New Insights Into Protein Release.","authors":"Ruey-Hwa Chen, Antonio J Costa-Filho, Jayanta Debnath, Thierry Galli, Liang Ge, Deborah Goberdhan, Wei Guo, Kangmin He, Ralf Jacob, Tiebang Kang, Min Goo Lee, Lian Li, Fabio Lolicato, Jun Lu, Vivek Malhotra, Walter Nickel, Vassiliki Nikoletopoulou, Stacey K Ogden, Georgia Maria Sagia, Feng Shao, Anbing Shi, Clotilde Thery, Christel Vérollet, Julien Villeneuve, Frederik Verweij, Yanzhuang Wang, Juan Wang, Shenjie Wu, Yihong Ye, Hang Yin, Li Yu, Min Zhang, Ying Zhang, Xin Zhou, Chiara Zurzolo","doi":"10.1111/tra.70022","DOIUrl":"10.1111/tra.70022","url":null,"abstract":"In eukaryotes, protein secretion plays essential roles in intercellular communications and extracellular niche-building. Protein secretion generally requires a signal sequence that targets cargos to the canonical secretory pathway consisting of the endoplasmic reticulum (ER), the Golgi apparatus, plasma membrane, and vesicles moving between these compartments. However, cytoplasmic proteins lacking signal sequences (e.g., IL1β, Acb1, FGF2) have been detected, and many have defined functions in the extracellular space, suggesting unconventional protein secretion (UcPS) via alternative pathways. In recent years, scientists have uncovered many new UcPS paradigms, reporting a plethora of mechanisms that collectively form a new field. The inaugural Cold Spring Harbor Asia (CSHA) conference on \"Molecular Mechanisms and Physiology of Unconventional Secretion\" is the first meeting to bring these researchers together, providing a collegial platform for information sharing at this exciting frontier of cell biology research.","PeriodicalId":23207,"journal":{"name":"Traffic","volume":"26 10-12","pages":"e70022"},"PeriodicalIF":2.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12590341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145453236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Small Extracellular Vesicles in Tumor Development and the Prospect of Targeted Therapy. 细胞外小泡在肿瘤发展中的作用及靶向治疗的前景。

IF 2.5 3区生物学

Traffic Pub Date : 2025-10-01 DOI: 10.1111/tra.70023

Yinan Yu, Shengjie Wang, Rong Ji, Gaoyang Gu, Fuping Zhang, Jianfang Wang, Xialin Chen

引用次数: 0

From Mechanisms to Therapy: Exploring the Role of Ferroptosis in Cervical Cancer Transformation and Treatment. 从机制到治疗：探讨铁下垂在宫颈癌转化和治疗中的作用。

IF 2.5 3区生物学

Traffic Pub Date : 2025-07-01 DOI: 10.1111/tra.70018

Zhenlei Wang, Yuanyuan Xiao, Ranzhong Chen, Erqun Tang, Shuangyang Tang

{"title":"From Mechanisms to Therapy: Exploring the Role of Ferroptosis in Cervical Cancer Transformation and Treatment.","authors":"Zhenlei Wang, Yuanyuan Xiao, Ranzhong Chen, Erqun Tang, Shuangyang Tang","doi":"10.1111/tra.70018","DOIUrl":"10.1111/tra.70018","url":null,"abstract":"Cervical cancer (CC) exerts a considerable impact on women's health worldwide and presents persistent challenges to conventional therapeutic strategies due to its propensity for distant metastasis, postoperative recurrence, and variable drug resistance. Ferroptosis, a recently identified type of programmed cell death, offers promising potential for a therapeutic approach for CC. This paper reviews the regulatory processes involved in ferroptosis, including the sequential events leading to cell membrane rupture via lipid peroxidation and the changes in ferroptosis sensitivity as cervical cells progress from a healthy to a malignant condition. Additionally, the dynamic relationship between ferroptosis and CC transformation driven by high-risk HPV (HR-HPV) infection is examined, with a particular focus on how HR-HPV E6/E7 proteins influence ferroptosis sensitivity. By examining the factors associated with ferroptosis, this review provides insights into CC progression and prognosis. Furthermore, therapeutic strategies targeting ferroptosis are discussed, offering novel perspectives for effective treatment options for CC.","PeriodicalId":23207,"journal":{"name":"Traffic","volume":"26 7-9","pages":"e70018"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Traffic最新文献