{"title":"Screening of infection conditions for brain microvascular endothelial cells infected by Streptococcus suis","authors":"Mingcheng Liu, O. Kasianenko","doi":"10.32718/ujvas5-2.04","DOIUrl":"https://doi.org/10.32718/ujvas5-2.04","url":null,"abstract":"Streptococcus suis is a pathogen that causes swine meningitis, sepsis, and other diseases. There are 34 serotypes, of which type 2 is the most pathogenic. During the infection process of Streptococcus suis, several major virulence factors are involved and play a different roles. Streptococcal meningitis is caused by the bacteria’s ability to cross the blood-brain barrier and enter the central nervous system. Therefore, studying the interaction between Streptococcus suis and cerebral microvascular endothelial cells will help reveal meningitis's pathogenic mechanism. When studying the interaction between bacteria and cells, the number of infected bacteria and the time of infection are very important. In this study, Streptococcus suis serotype two was made into bacteria liquid and counted. Then bacteria were used to infect mouse brain microvascular endothelial cells with different multiplicity of infection (1,10,100 and 200). Cells were harvested at six h, nine h,12h,18h, and 24h after infection. The total RNAs of harvested cells were extracted, and the concentration of RNA was detected. The OD260/OD280 was between 1.8~2.4, OD260/OD230 was 1.5~2.4, and the concentration was greater than 100ng/µL. Total RNAs were reverse transcribed to cDNAs used to perform quantitative PCR to detect the mRNA expression of IL-18, IL-1beta, IL-6, and IL-10. The results showed that each MOI group's mRNA expression is higher than the control group with different infection times. When the multiplicity of infection is at 1, each group's relative expression of cytokines reaches a peak at 18hrs after infection. When the multiplicity of infection is at 10, each group's relative expression of cytokines reaches a peak at 12hrs after infection. When the multiplicity of infection is at 100, the relative expression of cytokines reaches a peak at 12hrs after infection. When the multiplicity of infection is at 200, the relative expressions of each cytokine reach a peak at 6hrs after infection. Based on the mRNA relative expression of each cytokine under different conditions, the optimal multiplicity of infection was 100, and the optimal infection time was 12h. The result provides a basis for the study of the pathogenic mechanism of meningitis.","PeriodicalId":231752,"journal":{"name":"Ukrainian Journal of Veterinary and Agricultural Sciences","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130826050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Zhyla, N. Shkodyak, O. Pyatnychko, G. Kotsyumbas, N. Lisova, O. Sobodosh, M. Shkil, E. Khmil
{"title":"Blood indices and immune organs morphological structure of broiler chickens under the influence of different doses of probiotic feed additives","authors":"M. Zhyla, N. Shkodyak, O. Pyatnychko, G. Kotsyumbas, N. Lisova, O. Sobodosh, M. Shkil, E. Khmil","doi":"10.32718/ujvas5-2.03","DOIUrl":"https://doi.org/10.32718/ujvas5-2.03","url":null,"abstract":"In the development of effective methods of prevention and treatment of poultry today, importance is given to probiotics based on microbial cultures, in particular, lactobacilli, bifidobacteria, yeast, etc. In contrast to antibiotics, the use of probiotics stimulates the immune response of animals, restores the microflora of the gastrointestinal tract, and ensures its optimal ratio. At the same time, livestock products remain safe for the consumer. The purpose of our work was to conduct comparative clinical trials of the efficiency of the probiotic feed additive Probion-forte in terms of productivity, blood indices, and histological structure of immune organs of chicken broilers during fattening. The clinical studies were carried out with broiler chickens of “Cobb-500” cross at the age of 2 days, which were formed into four groups (300 units in each one). In order to determine the efficiency of application, probiotic feed additives were given to chickens in different concentrations during the growing period, together with the main diet. The first group (1st) was given probiotic Probion-forte in the dose of 1 g/kg of feed, the second group (2nd) – Probion-forte in a dose of 0.5 g/kg, the third (3rd) one – a probiotic-analog “Bio plus 2B” in a dose of 0.4 g/kg; the forth (4th) group was a control group – chickens received an essential diet, without adding any feed additives. The compound feed was provided according to norms recommended for the cross “Cobb-500”, considering age. On the 15th, 30th, and 43rd day of the test, 20 units were selected for hematological, pathomorphological and microbiological tests. The productivity of birds of all studied groups was evaluated by the average daily gains, safety, feed conversion, and slaughter output. The stabilized with EDTA chicken blood was used for morphological studies, and blood serum – for biochemical and immunological studies. Comparative clinical studies have shown that adding to the main diet of broiler chickens probiotic feed additive Probion-forte at a dose of 0.5 and 1.0 g/kg throughout the growing period increases the safety and improves feed digestibility, and increases the bird’s European Efficiency Index. Based on the obtained hematological, biochemical, and immunological parameters of blood, it can be argued about the activating effect on the body of broiler chickens probiotic feed additives, namely the intensification of reanimation of free amino acids, which increases the content of the studied metabolites of lipid metabolism, which chickens use as energy and plastic material. Stimulating cellular and humoral protection, nonspecific resistance is established, confirmed by a high level of lysozyme activity in blood serum and significantly higher content of T- and B-lymphocytes, NK-cells, and γ-globulins. The macro- and microscopic structure of the studied immune organs is preserved in all groups of broiler chickens. Compared with the control group, morphometric examination in the immune or","PeriodicalId":231752,"journal":{"name":"Ukrainian Journal of Veterinary and Agricultural Sciences","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122302027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Katsaraba, R. Sachuk, B. Gutyj, T. Velesyk, M. L. Radzykhovskyi, P. Sharandak, V. Pepko
{"title":"Pharmacological studies of the veterinary medicinal product “Dibutalastin Ointment”","authors":"O. Katsaraba, R. Sachuk, B. Gutyj, T. Velesyk, M. L. Radzykhovskyi, P. Sharandak, V. Pepko","doi":"10.32718/ujvas5-2.07","DOIUrl":"https://doi.org/10.32718/ujvas5-2.07","url":null,"abstract":"The study of pharmacological studies of the veterinary medicinal product for external use in the form of ointment – “Dibutalastin Ointment” is a mandatory stage of preclinical research of the drug, which is a new development of PP “Biopharm” and LLC “DEVIE”. In a scientific experiment on laboratory animals, it is possible to assess the drug's safety for different terms of use to determine the zone of toxic action and doses that do not cause harmful effects on health. Therefore, the work aimed to carry out pharmacological studies of the drug with methyl salicylate and dimethyl sulfoxide in the form of an ointment for treating European fallow deer, deer, dogs, and cats with mastitis, arthritis, myositis, and injuries of various origins. The toxicological characteristics of the studied ointment “Dibutalastin” based on methyl salicylate and dimethyl sulfoxide were studied in an \"acute\" study on warm-blooded animals. The average lethal dose (LD50) for intragastric administration could not be established, as the volume exceeded the permissible level. It was established that according to the “average lethal dose when entering the stomach” indicator, the veterinary medicinal product belongs to relatively non-toxic substances (toxicity class IV). The results of pharmacological studies indicate the absence of resorptive-toxic effects, sensitizing properties, and irritating effects on the mucous membranes of the eyes and skin. Pathomorphological studies of animal organs when studying the acute toxicity of the drug showed that the developed ointment with methyl salicylate and dimethyl sulfoxide does not cause pathological changes in internal organs. From the point of view of veterinary toxicology, this drug is safe. Further studies will be the next stage of pre-registration tests aimed at studying the embryotoxic effect of “Dibutalastin Ointment”, which is mandatory material of the “Safety and residue studies” section of the dossier for this medicinal product.","PeriodicalId":231752,"journal":{"name":"Ukrainian Journal of Veterinary and Agricultural Sciences","volume":"357 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132881602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Kushnir, I. Kushnir, I. Patereha, O. Kutsan, O. Zhovnir, B. Gutyj
{"title":"Comparative assessment of various methods of studying the skin toxicity of a wound-healing drug","authors":"V. Kushnir, I. Kushnir, I. Patereha, O. Kutsan, O. Zhovnir, B. Gutyj","doi":"10.32718/ujvas5-2.01","DOIUrl":"https://doi.org/10.32718/ujvas5-2.01","url":null,"abstract":"The article presents the results of studying the subacute skin toxicity of a wound-healing cream based on betamethasone dipropionate, gentamicin sulfate, and clotrimazole. According to the Organization for Economic Cooperation and Development (OECD No. 410), the research was conducted using the classic method. When studying the effect of a wound-healing agent on the body of animals during long-term 28-day dermal application by the classical method in animals of the I, II, and III experimental groups, which received the drug in a dose of 0.5, 2.5, and 5 ml/kg body weight; respectively no significant changes were found in the body weight of the animals and the weight coefficients of the liver, heart, spleen, kidneys, and lungs. At the same time, in the animals of the II and III research groups, a slight increase in the concentration of hemoglobin was noted, respectively, by 1.3 and 3.9 %, the level of urea – 5.3 and 11.2 %, the activity of AST – 9.8 and 14.9 % compared to the indicators of animals of the control group. In these groups, there was a decrease in total protein concentration by 5.5 and 6.8 %, creatinine level by 3.1 and 5.3 %, and ALT activity by 6.5 and 9.7 %. The studies conducted according to the OECD No. 410 make it possible to obtain more informative data on the toxic effect of the investigated agent. In particular, it was established that the use of the researched drug in animals of the I, II, and III experimental groups caused a decrease in the weight coefficients of the spleen mass, respectively, by 19.1 (Р < 0.05), 22.4 (Р < 0.05) and 28.3 % (P < 0.05), hearts – 3.6, 4.6 and 7.7 %, kidneys – 4.9, 6.5 and 10.4 % and animal body weight – 4.5, 5.4 and 6.4 %, hemoglobin concentrations – 2.6, 2.9 and 4.4 %, leukocyte counts – 21.4, 16.1 and 15.1 %, hematocrit values – 1.9, 3, 6 and 4.0 %. The average hemoglobin content in the erythrocyte (MSN) also decreased by 8.3, 6.9, and 5.1 %, and the average erythrocyte (MCV) volume was 5.0, 4.7, and 1.7 %. In addition, an increase in weight coefficients of liver mass was established by 10.8 (P < 0.05), 11.8 (P < 0.05), and 15.6 % (P < 0.05). When studying the effect of the researched agent during 28-day dermal application on biochemical indicators of blood serum in animals of the first experimental group, a decrease in the concentration of total protein by 5.8 % was established. Under these conditions, an increase in creatinine level, urea level, ALT, and AST was established, respectively, by 6.2, 18.8 (P < 0.05), 3.8, and 14.7 % (P < 0.05). It should be noted that the use of the researched product in animals of the II and III experimental groups caused an increase in the level of urea, respectively, by 28.6 and 35.7 % (Р < 0.05), creatinine by 8.4 and 3.5 %, AST – 16.4 (P < 0.05) and 11.3 % and the activity of ALT – 8.5 and 11.0 %.","PeriodicalId":231752,"journal":{"name":"Ukrainian Journal of Veterinary and Agricultural Sciences","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116934663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dependence of the artificial reservoir pollution with heavy metals on anthropogenic factors","authors":"V. Palamarchuk, H. Shpakovska, A. Kolechko","doi":"10.32718/ujvas5-1.05","DOIUrl":"https://doi.org/10.32718/ujvas5-1.05","url":null,"abstract":"The article presents the research results of the heavy metals content in artificial reservoirs located at different distances from a number of pollutants of anthropogenic origin. The manuscript provides data on the dependence of the concentration of copper, zinc, lead, iron, cadmium in pond water on their location on highways and arable land. The location near these anthropogenic factors of artificial reservoirs pollution causes the accumulation of heavy metals in the water leading to significant exceedances of permissible levels for the catch of commercial fish, poses a significant threat to the environmental situation and safety of aquaculture. Research has also revealed that fish farming technology used in the farm also determines the ingress and accumulation of heavy metals in the reservoir. In particular, it was found that the operation of non-letting ponds, the use of cascades near the highway and arable land increases the accumulation of heavy metals. According to the results of our research, the content of heavy metals in a pond located at a short distance from the road and agricultural land indicate a high increase in the concentration of toxicants in water. It was established that the concentration of Kuprum was in the water thickness – 0.085 mg/dm3, and at the bottom of the reservoir 0.095 mg/dm3 at MPC 0.001 mg/dm3. The Zn content in the water thickness was 0.119 mg/dm3, in the deep layers of the reservoir – 0.127 mg/dm3, exceeding the maximum allowable concentrations of 11.9 and 12.7 times, respectively. Mangan in the water thickness of the reservoir located near anthropogenic polutantes was 0.091 mg/dm3, and near the bottom of the reservoir – 0.034 mg/dm3 at the MPC – 0.01 mg/dm3. Fe in the water of an artificial reservoir located near the field and road exceeded the maximum allowable concentrations for this chemical element by 20 times regardless of the layer of its selection","PeriodicalId":231752,"journal":{"name":"Ukrainian Journal of Veterinary and Agricultural Sciences","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116030841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Horalskyi, I. Sokulskyi, B. Gutyj, I. Y. Goralskaya, N. Kolesnik
{"title":"Pathogenetic aspects of retroviral infections","authors":"L. Horalskyi, I. Sokulskyi, B. Gutyj, I. Y. Goralskaya, N. Kolesnik","doi":"10.32718/ujvas5-1.10","DOIUrl":"https://doi.org/10.32718/ujvas5-1.10","url":null,"abstract":"Bovine leukemia and equine infectious anemia are registered in most regions of Ukraine and in many countries worldwide. This is accompanied by economic losses and reduced quality of livestock products, which determines the relevance of the study of retroviral infections, their diagnosis, and pathogenesis. The goal of our research was to find and improve the methodological foundations of the development of the pathological process for a more in-depth study of the etiology, pathogenesis, treatment, and prevention of retroviral infections. The object of the study was blood, lymph nodes, spleen, heart, lungs, liver, and kidneys for bovine leukemia and equine infectious anemia. Hematological, anatomical, histological, histochemical, morphometric, and statistical research methods were used for their study. The research group included 304 cattle aged 4–9 years and 42 horses, of which 25 were infected with the virus, and 17 were in the control group. It has been established that bovine leukemia and infectious anemia of horses is an irreversible pathological process characterized by slow progression, the presence of a latent or persistent form, with damage to cells, organs, and systems of the body, which leads to death. Infectious anemia of horses differs from leukemia of cattle by the hidden course of the pathological process. The pathogenesis of leukemia occurs in six stages, which we have identified and conventionally named.","PeriodicalId":231752,"journal":{"name":"Ukrainian Journal of Veterinary and Agricultural Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130963040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Chechet, V. Kovalenko, O. Vishchur, O. Haidei, N. Liniichuk, B. Gutyj, O. Krushelnytska
{"title":"The activity of T- and B-cell links of specific protection of chicken-broilers under the influence of synbiotic preparation “Biomagn” and “Diolide” disinfectant","authors":"O. Chechet, V. Kovalenko, O. Vishchur, O. Haidei, N. Liniichuk, B. Gutyj, O. Krushelnytska","doi":"10.32718/ujvas5-1.08","DOIUrl":"https://doi.org/10.32718/ujvas5-1.08","url":null,"abstract":"One of the most actual problems of modern poultry farming is to increase the preservation of chickens and ensure high intensity of their growth at all stages of rearing. The development of alternative methods of prevention and treatment of infectious diseases of poultry involves the use of preparations of natural or organic origin, which have antagonistic properties against infectious agents and are able to balance the immune response. A prominent place in the system of disease prevention and increasing the resistance of animals and poultry is the development of modern probiotic and bactericidal preparations. In view of this, we studied the influence of the synbiotic preparations “Biomagn” in combination with the disinfectant “Diolide” on the state of the T- and B-cell links of the specific immunity of broiler chickens during the period of their rearing. The preparation “Biomagn” is based on representatives of the normal commensal microflora - non-pathogenic lactic acid bacteria with antibacterial and immunomodulatory properties and additional components: magnesium chloride, chitosan, xylanase, protease, cellulase, milk thistle meal, acidity regulator, betaine and emulsifier. The specified remedy is used to ensure optimal level of metabolism, increase immune function, growth, safety of animals and poultry. The results of the research showed that the use of the synbiotic preparation “Biomagn” in combination with the disinfectant “Diolide” had a positive influence on the activity of T- and B-cell links of specific defense of broiler chickens organism during the period of their rearing. This is evidenced by an increase in the amount of T-lymphocytes (total, active and theophylline-resistant) and B-lymphocytes in the blood and an increase in their functional activity due to the redistribution of the receptor apparatus of immunocompetent cells. In particular, a decrease in the number of functionally inactive T- and B-lymphocytes in the blood and an increase in the number of cells with low and medium degree of avidity. Therefore, the use of a set of studied immunotropic preparations is a promising direction in increasing the resistance and productivity of poultry. This allows in industrial conditions of maintenance, without loss of productivity, to respond to technological factors that can lead to immunodeficiency, disease and death of poultry.","PeriodicalId":231752,"journal":{"name":"Ukrainian Journal of Veterinary and Agricultural Sciences","volume":"105 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133775252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Fotina, A. Berezovsky, R. Petrov, O. Shkromada, A. Nechiporenko, O. Fotin, P. Bondarenko
{"title":"Changes in the chemical composition of broiler meat when chelated compounds are added to the diet","authors":"T. Fotina, A. Berezovsky, R. Petrov, O. Shkromada, A. Nechiporenko, O. Fotin, P. Bondarenko","doi":"10.32718/ujvas5-1.07","DOIUrl":"https://doi.org/10.32718/ujvas5-1.07","url":null,"abstract":"The paper considers the use of chelated forms of micronutrients for feeding broilers. The study aims to investigate the chemical composition of broiler meat in the case of broiler chickens of Cobb-500 cross, provided Zn, Cu, and Mn chelated forms are supplied to the diet. Experimental studies were conducted in 2020 on broiler chickens of Cobb-500 cross. Two groups of 20 birds were formed to study the chemical composition of poultry meat. The birds of the control group received an essential diet supplied with sulfates of Zn, Cu, and Mn. The birds of the experimental group received a diet enriched with chelated compounds of Zn, Cu, and Mn. The study has shown that introducing Zn, Cu, and Mn chelated compounds into the diet of broiler chickens has no adverse effect on the chemical composition of meat. It has also been determined that the meat of broilers eating feed supplied with chelated micronutrients contains significantly less cholesterin but more Ca, Zn, Cu, and Mn, and several essential amino acids. These indicators prove an increase in the health benefits of chicken meat.","PeriodicalId":231752,"journal":{"name":"Ukrainian Journal of Veterinary and Agricultural Sciences","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115310001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ovocidal action of disinfectants against eggs of Passalurus ambiguus","authors":"A. Khorolskyi","doi":"10.32718/ujvas5-1.09","DOIUrl":"https://doi.org/10.32718/ujvas5-1.09","url":null,"abstract":"Successful elimination of helminthic diseases, as well as their further prevention, is possible only under the condition of carrying out a complex of organizational and economic, veterinary and sanitary, and special anti-parasitic measures, which must necessarily include disinfestation of places where animals are kept, as well as environmental objects. It is necessary to establish the ability of modern chemical agents to harm certain types of parasites. The research aimed to determine the ovocidal efficiency of disinfectants in different concentrations and exposures against Passalurus ambiguus eggs isolated from domestic rabbits. An experimental test was carried out in laboratory conditions on chemical products of domestic production “Virosan”. (AS – alkyldimethylbenzylammonium chloride, glutaraldehyde) and “Hermecid-VS” (AS – didecyldimethylammonium chloride, glutaraldehyde, benzalkonium chloride). The leading indicator of the effect of disinfectants on the eggs of pasaluris was the value of their disinfestation efficiency. The results of the conducted studies established a high level of ovocidal efficiency of “Virosan” (OE – 100 %) against P. ambiguus eggs at 0.25 % concentration for exposures of 30–60 minutes and 0.5 % concentration for exposures of 10–60 minutes. A satisfactory level of the ovocidal efficiency of “Virosan” was established when using the agent in 0.1 % (30–60 min exposure) and 0.25 % (10 min exposure) concentrations, OE ranges from 62.36 to 82.20 %. An unsatisfactory level of the ovocidal efficiency of “Virosan” was established at 0.1 % of the concentration after exposure for 10 minutes (AS – 47.30 %). Disinfectant “Hermecid-VS” showed a high level of ovocidal efficiency concerning the eggs of pasaluris in 0.1 % concentration for 60 min exposure (OE – 91.45 %) and in 0.25–0.5 % concentration for 10–60 min exposure (AS – 100 %). A satisfactory ovocidal efficiency of “Hermecid-VS” was established when using the product at a concentration of 0.1 % (10 and 30-minute exposures), where OE indicators were 76.25 and 81.91 %, respectively. The obtained data make it possible to recommend disinfectants of domestic production “Virosan” and “Hermecid-VS” in specified concentrations and exposures to control and prevent pasalurosis in rabbits in order to effectively carry out the deinvasion of environmental objects and places where animals are kept.","PeriodicalId":231752,"journal":{"name":"Ukrainian Journal of Veterinary and Agricultural Sciences","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133513558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Level of phenotypic manifestation of feeding and meat qualities of young pigs of different intrabreed differentiation according to some multi-component evaluation indexes","authors":"V. Khalak, B. Gutyj","doi":"10.32718/ujvas5-1.11","DOIUrl":"https://doi.org/10.32718/ujvas5-1.11","url":null,"abstract":"The paper deals with the results of the search for fattening and meat qualities of young pigs of Large White breeds of different interbreeding differentiation according to some integrated indicators, the level of correlations between traits, and the economic efficiency of research results is calculated. The experimental part of the work was performed in agricultural formations of the Dnipropetrovsk region and the laboratory of animal husbandry of the State Establishment Institution “Institute of Grain Crops of NAAS of Ukraine”. Evaluation of young pigs for fattening and meat qualities was carried out taking into account the following indicators: average daily gain of live weight during the period of control fattening, kg, age achievement of live weight 100 kg, days, the thickness of the lard at the level of 6–7 thoracic vertebrae, mm, the length of the chilled carcass, cm. A comprehensive evaluation of young pigs for fattening and meat qualities was performed according to the Tyler and Sazer-Fredin indices. Economic efficiency of research results and biometric processing of the obtained data were carried out according to generally accepted methods. It was found that young pigs of the Large White breed of the controlled population at the age of 100 kg exceeded the minimum requirements of the elite class by 6.13%, the thickness of the lard at the level of 6-7 thoracic vertebrae – 32.25 % and the length of the chilled carcass – 3.92 %. A significant difference between animals of different interbreeding differentiation according to the Tyler index (groups I and III) was set up by the average daily gain of live weight during the control period of fattening (91.7 g), age of 100 kg live weight (7.3 days), lard thickness at the level of 6–7 thoracic vertebrae (3.4 mm), length of the chilled carcass (1.7 cm), length of bacon half of chilled carcass (2.7 cm). Taking into account the interbreed differentiation according to the Sazer-Fredin index, it is found that young pigs of the II group prevailed over their peers of I in terms of average daily increase in live weight during the period of control fattening by 4.03 %, age of achievement of live weight of 100 kg – 3.67 % and length of the chilled carcass – 0.2 %. The pairwise correlation coefficient between fattening and meat qualities of young pigs and evaluation indices ranges from -0.710 (tr = 10.24) to +0.844 (tr = 20.98). The maximum increase in additional products was obtained from young pigs of the first experimental group according to the Tyler index (+5.03 %) and the second experimental group according to the Sazer-Fredin index (+1.12 %). The cost of additional products got from the young pigs of these groups, provided that the selling price per 1 kg of live weight of young pigs at the time of the search was 47.5 UAH is +247.51 and +55.42 UAH/head, respectively.","PeriodicalId":231752,"journal":{"name":"Ukrainian Journal of Veterinary and Agricultural Sciences","volume":"177 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132828329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}