Trakya University Journal of Natural Sciences最新文献_第8页

Distinctive SIRE1 Retrotransposon Patterns on Barley Chromosomes? 大麦染色体上独特的SIRE1反转录转座子模式?

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Trakya University Journal of Natural Sciences Pub Date : 2020-10-09 DOI: 10.23902/trkjnat.773302

E. Karlik, N. Gozukirmizi

{"title":"Distinctive SIRE1 Retrotransposon Patterns on Barley Chromosomes?","authors":"E. Karlik, N. Gozukirmizi","doi":"10.23902/trkjnat.773302","DOIUrl":"https://doi.org/10.23902/trkjnat.773302","url":null,"abstract":": SIRE1 is an active and relatively high copy-number retroelement belongs to the Tyl/Copia long terminal repeat (LTR) retrotransposon superfamily. Distinctive SIRE1 elements ( ENV and GAG ) distributions in barley genome were observed by using fluorescent in situ hybridization (FISH). We performed PCR to obtain tetramethylrhodamine-dUTP (TRITC)-labelled probes. Localizations of SIRE1 ENV and GAG domains were demonstrated under confocal microscope on Hordeum vulgare L. cv. Hasat root preparations. Our results revealed the distributions of SIRE1 elements ENV and GAG in barley genome. These results may provide to uncover the organization of SIRE retrotransposon pattern in barley genome. Özet: SIRE1 , Tyl/Copia Uzun Uç Tekrarlı (Long Terminal Repeats- LTR) retrotranspozon üst ailesine ait olan aktif, nispeten yüksek kopyalı bir retroementtir. Arpa genomundaki ayırt edici SIRE1 elementlerinin ( ENV ve GAG ) dağılımları floresan in situ hibridizasyonu (FISH) kullanılarak gözlemlendi. Tetramethylrhodamine-dUTP (TRITC)-işaretli probların elde edilmesinde PCR gerçekleştirildi. SIRE1 ENV ve GAG domainlerinin yerleşimleri, Hordeum vulgare L. cv. Hasat kök preparatlarında konfokal mikroskobu altında gösterildi. Sonuçlarımız, arpa genomundaki SIRE1 elementlerinin ENV ve GAG dağılımlarını göstermektedir. Bu sonuçlar, SIRE1 elementlerinin arpa genomunun organizasyonunun ortaya çıkarılmasına katkı sağlayacaktır.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2020-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83107368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

USE OF MULBERRY POMACE AS SUBSTRATE FOR CITRIC ACID PRODUCTION BY Aspergillus niger MT-4 桑树渣作为黑曲霉MT-4产柠檬酸底物的研究

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Trakya University Journal of Natural Sciences Pub Date : 2020-10-02 DOI: 10.23902/trkjnat.670859

Rufina Aidynova, N. Arslan, M. Aydoğan

{"title":"USE OF MULBERRY POMACE AS SUBSTRATE FOR CITRIC ACID PRODUCTION BY Aspergillus niger MT-4","authors":"Rufina Aidynova, N. Arslan, M. Aydoğan","doi":"10.23902/trkjnat.670859","DOIUrl":"https://doi.org/10.23902/trkjnat.670859","url":null,"abstract":"Dut posasi (DP), geleneksel bir Turk yiyecegi olan pekmez'in uretiminden sonra ortaya cikan bir atik maddedir. Bu calisma, Aspergillus niger MT-4 ile sitrik asit (SA) uretimi icin DP’nin substrat olarak kullanilabilirligini test etmek icin gerceklestirilmistir. Calismada ayrica, SA uretimini artirmak icin bazi kultur kosullari da optimize edilmistir. MP’nin nem, toplam karbonhidrat, suda-cozunur karbonhidrat, protein, lipid ve kul icerikleri sirasiyla %31,1; 47,1; 1,3; 13,4; 1,8 ve 1,4 olarak belirlenmistir. Deneyler, uretim besiyerinin 100 ml’sini iceren 250 ml'lik erlenler icerisinde gerceklestirilmistir. Hem fungal biyokutle (FB) hem de SA uretimi icin optimum DP konsantrasyonu, 120 gr/lt olarak belirlenmistir. DP bazli ortama eklenen tum KH2PO4 konsantrasyonlarinin SA uretimini azalttigi fakat FB uretimini artirdigi belirlenmistir. SA uretimi icin MgSO4 ve (NH4)2SO4’un optimal konsantrasyonlari sirasiyla 1 ve 2 gr/lt olarak bulunmustur. Diger optimal parametreler, baslangic pH'si 7,0 ve inkubasyon suresi 5 gun olarak belirlenmistir. Optimize edilmis kultur kosullari altinda, uretilen SA miktari 24,6 gr/lt olarak belirlenmistir. Besinci gunde, Yp/s; Yp/x ve Yx/s sirasiyla 0,2 gr SA/gr DP; 1,43 gr SA/gr FB ve 0,14 gr FB/gr DP olarak hesaplanmistir. DP'nin SA dahil mikrobiyal metabolitlerin uretimi icin fermentasyon substrati olarak kullanilabilirligi ilk kez bu calismada test edilmistir.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2020-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76733872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

CONSERVATION STATUS OF THREE RARE AND ENDEMIC SPECIES FROM TURKEY (KALIDIUM WAGENITZII, MUSCARI ADILII AND VERBASCUM GYPSICOLA) 土耳其3种珍稀特有物种(wagenitzii kalidium、muscari adilii和verbascum gypsicola)的保护现状

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Trakya University Journal of Natural Sciences Pub Date : 2020-09-23 DOI: 10.23902/trkjnat.751851

Ali Murat Keser, G. Ayyildiz, Merve Yıldırım, A. Yaprak, G. Tug

{"title":"CONSERVATION STATUS OF THREE RARE AND ENDEMIC SPECIES FROM TURKEY (KALIDIUM WAGENITZII, MUSCARI ADILII AND VERBASCUM GYPSICOLA)","authors":"Ali Murat Keser, G. Ayyildiz, Merve Yıldırım, A. Yaprak, G. Tug","doi":"10.23902/trkjnat.751851","DOIUrl":"https://doi.org/10.23902/trkjnat.751851","url":null,"abstract":"In this study, we aimed to determine the population size and distribution areas and to re-evaluate IUCN threat categories of Kalidium wagenitzii (Aellen) Freitag & G. Kadereit, Muscari adilii M.B. Guner & H. Duman and Verbascum gypsicola Vural & Aydogdu, which are endemics of the Central Anatolia region of Turkey. The three species have limited distribution areas and high risk of extinction as a result of human impact. The perennial halophytic species K. wagenitzii was found in 5 localities around Salt Lake (Tuz Golu) and a total number of 6458 mature individuals were determined. The area of occupancy of the species is 36 km2 and the extent of occurrence is 213 km2. Uncontrolled use of water resources for agricultural purposes is a serious threat factor for the species. Kalidium wagenitzii is listed in EN category according to the IUCN Red List criteria. Muscari adilii prefers marly soils and its habitat type is formed by secondary succession after the destruction of Pinus nigra and oak forests. It is known from 3 localities around Nallihan-Beypazari with a population size of 6144 mature individuals. The area of occupancy and the extent of occurrence of the species is 12 km2 and 28 km2, respectively. The pressures on the species are road construction and increase of farmland, afforestation and factory establishment. According to the IUCN Red List criteria, the species is listed in CR category. Verbascum gypsicola is distributed on marly soils. It is known from 3 localities around Nallihan–Beypazari and one locality in Sivrihisar-Eskisehir with 2755 mature individuals in total. The area of occupancy of the species is 16 km2 and the extent of occurrence is 269 km2. Overgrazing and expansion of agricultural land, together with factory establishment are the major threats for this species, which is listed as EN in IUCN Red List.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2020-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78582103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

THE COMMERCIAL AND DISCARD CATCH RATES OF THE TRAWL FISHERY IN THE ISKENDERUN BAY (NORTHEASTERN LEVANTINE SEA) 伊斯肯德伦湾(东北黎凡特海)拖网渔业的商业捕捞率和废弃捕捞率

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Trakya University Journal of Natural Sciences Pub Date : 2020-09-18 DOI: 10.23902/TRKJNAT.773435

C. Dalyan

引用次数: 3

NOTES ON LEAF AND STEM ANATOMY OF Thlaspi Sensu Lato 标题海苔叶茎解剖注释

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Trakya University Journal of Natural Sciences Pub Date : 2020-09-16 DOI: 10.23902/TRKJNAT.762509

Cengiz Karaismailoğlu, Osman Erol

引用次数: 3

AGE, GROWTH AND OTOLITH BIOMETRY-BODY LENGTH RELATIONSHIPS OF RED BANDFISH (Cepola macrophthalma L., 1758) IN THE SEA OF MARMARA, TURKEY 土耳其马尔马拉海红带鱼(Cepola macrophthalma L.， 1758)的年龄、生长和耳石生物特征-体长关系

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Trakya University Journal of Natural Sciences Pub Date : 2020-09-11 DOI: 10.23902/trkjnat.714201

A. İşmen, M. A. İhsanoğlu, I. B. Daban, H. Inceoğlu

{"title":"AGE, GROWTH AND OTOLITH BIOMETRY-BODY LENGTH RELATIONSHIPS OF RED BANDFISH (Cepola macrophthalma L., 1758) IN THE SEA OF MARMARA, TURKEY","authors":"A. İşmen, M. A. İhsanoğlu, I. B. Daban, H. Inceoğlu","doi":"10.23902/trkjnat.714201","DOIUrl":"https://doi.org/10.23902/trkjnat.714201","url":null,"abstract":"Cepola macrophthalma (Linnaeus, 1758)’in yas, buyume ve otolit biyometri-total boy iliskileri arastirilmistir. Bireyler Mart 2012-Haziran 2014 tarihleri arasinda Marmara Denizi'nde algarna ile yakalanmistir. Bu calismada orneklenen 51,5 cm'lik C. macrophthalma bireyinin boyu, Marmara Denizi'nde bu tur icin maksimum olarak kaydedilmistir. Boy-agirlik iliskisi denklemine gore buyume tipi negatif allometrik olarak belirlenmistir (b=1,36). Otolith boyu-otolith agirligi, otolith genisligi- otolith agirligi, otolith boyu-total boy, otolith genisligi-total boy, otolit boyu-otolit genisligi, total boy-otolit agirligi iliskileri OW=0,0002*OL2,6377 (R2=0,91), OW=0,001*OWi2,6215 (R2=0,94), OL=0,057*TL+3,2087 (R2=0,57), OWi=0,0316*TL+1,8511 (R2=0,55), OWi=0,527*OL+0,2015 (R2=0,86) OW=0,0004*TL+0,0029 (R2=0,58) olarak hesaplanmistir. Yaslar 80 adet otolit okumasindan hesaplanmistir; gozlemlenen en kucuk ve en buyuk yaslar sirasiyla 1 ve 5’tir. von Bertalanffy denklemine gore hesaplanan buyume parametreleri degerleri L∞=61,95 cm, K=0,19 yil-1, t0 = -0,05 yil olarak belirlenmistir. Stogun buyuk cogunlugu (%77) genc bireylerden (1-2 yas grubu) olusmaktadir. Marmara Denizi’nde C. macrophthalma stogunun daha cok genc bireylerden olusmasi stoklarin av baskisi altinda oldugunu dusundurmektedir.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2020-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78983134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pioneering In vitro Studies for Callus Formation of Colchicum chalcedonicum 玉髓秋水仙愈伤组织形成的体外研究

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Trakya University Journal of Natural Sciences Pub Date : 2020-09-04 DOI: 10.23902/trkjnat.776974

E. Karlik, Meltem Değer, Erdal Uzen, N. Gozukirmizi

{"title":"Pioneering In vitro Studies for Callus Formation of Colchicum chalcedonicum","authors":"E. Karlik, Meltem Değer, Erdal Uzen, N. Gozukirmizi","doi":"10.23902/trkjnat.776974","DOIUrl":"https://doi.org/10.23902/trkjnat.776974","url":null,"abstract":"Colchicum calcedonicum Azn, pek cok endemik bitki turunun goruldugu Turkiye'de yayilis gosteren endemik turlerden biridir. Toprak altinda uzun-oval sekilli sogani ile genellikle 3-4 yapraklidir. Kallus kulturu kullanarak endemik turlerin in vitro uretimi, bu turlerin korunmasinda umut verici bir calisma haline gelmistir. Bu calismanin amaci, in vitro C. chalcedonicum uretimi icin verimli kallus protokolunun olusturulabilmesidir. Explantlarin sterilizasyonunda, 20 dk %0.25 (w/v) civa klorur (HgCl2) kullanilmistir. Civa klorure ilaveten, yuzey sterilizasyonunda 30 dk boyunca Tween 80, %6,5 NaCl ile birlikte kullanilmistir. Bu calismada, 19 farkli besiyeri kullanilmis olup primer kallus olusumu 2,4-D (2 mg L-1), 2IP (0,5 mg L-1), %3 sukroz ve %0,05 aktif karbon iceren Murashige & Skoog bazal besiyerinde elde edilmistir. Calismamiz, aktif karbon kullaniminin primer kallus olusumunda etkili oldugunu gostermistir. Bu calisma, C. chalcedonicum’un primer kallus olusumu icin ilk rapordur. Bununla birlikte, calismamiz endemik tur olan C. chalcedonicum'un in vitro korunmasi ve kallus olusum protokolunun gelistirilmesinde oncu bir calismadir.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2020-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79169284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

BORON INCREASES THE VIABILITY OF HUMAN CANCER AND MURINE FIBROBLAST CELLS AFTER LONG TIME OF CRYOPRESERVATION 硼能提高人肿瘤细胞和小鼠成纤维细胞在长期冷冻保存后的活力

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Trakya University Journal of Natural Sciences Pub Date : 2020-08-26 DOI: 10.23902/trkjnat.758920

T. Hayal

{"title":"BORON INCREASES THE VIABILITY OF HUMAN CANCER AND MURINE FIBROBLAST CELLS AFTER LONG TIME OF CRYOPRESERVATION","authors":"T. Hayal","doi":"10.23902/trkjnat.758920","DOIUrl":"https://doi.org/10.23902/trkjnat.758920","url":null,"abstract":"Canli hucrelerin uzun sure boyunca cok dusuk sicakliklarda saklanmasi islemi kriyo-korunma olarak adlandirilir. Kriyo-koruma islemi biyoteknoloji, klinik calismalar ve hayvan veya bitki hucreleriyle ilgili bircok calismada cok onemli bir rol oynadigindan dolayi, kriyo-korumada kullanilan ajanlarin arastirilmasi son derece muhimdir. Kriyo- koruma ajanlari, hucresel metabolizma ve biyofizyoloji uzerindeki etkileri nedeniyle uzun sureli kriyo-korumanin ardindan hucresel canliligin korunmasini saglarlar. Mevcut calismada; hucre canlilik testi, koloni olusturma testi ve gercek zamanli polimeraz zincir reaksiyonu tekniklerinden yararlanilarak, borun kriyo-koruma uzerindeki etkisi, insan akciger kanser hucre hatti, A549 ve fare fibroblast hucre hatti, L929 kullanilarak arastirilmistir. Hucre dondurma ortamini 15 µg/ml bor ile desteklemenin hucre canliligi uzerine olumlu etki ettigi gozlemlenmistir. Ayrica, tekrar eden dondurma - cozme donguleri ve uzun sureli kriyo-koruma sonucunda, gen anlatim profilleri degisen A549 ve L929 hucre hatlarinin, bor takviyesi sonrasinda, programli hucre olumuyle alakali genlerinin anlatiminda azalma, hucre cogalmasi ile ilgili genlerinde de artis gozlemlenmistir. Sonuclarimiz gostermistir ki bor kaynagi olarak sodium pentaborat pentahidrat, kanser veya saglikli hucrelerin canliliklarini kaybetmeksizin dondurulmalarini ve hucrelerin uzun sureli saklanmalari icin son derece onemli bir kriyo-koruyucu ajan olarak kullanilabilir.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2020-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89636305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

IMMOBILIZATION OF Bacillus subtilis E6-5 PROTEASE AND COMMERCIAL PROTEASE IN NANOFIBRILS CONTAINING DIFFERENT AMINO ACIDS 枯草芽孢杆菌E6-5蛋白酶和商品蛋白酶在含不同氨基酸纳米原纤维中的固定化

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Trakya University Journal of Natural Sciences Pub Date : 2020-04-01 DOI: 10.23902/trkjnat.633788

Baran Enes Guler, E. Demirkan, Tuba Sevgi̇

{"title":"IMMOBILIZATION OF Bacillus subtilis E6-5 PROTEASE AND COMMERCIAL PROTEASE IN NANOFIBRILS CONTAINING DIFFERENT AMINO ACIDS","authors":"Baran Enes Guler, E. Demirkan, Tuba Sevgi̇","doi":"10.23902/trkjnat.633788","DOIUrl":"https://doi.org/10.23902/trkjnat.633788","url":null,"abstract":"Bu calismada, elektrospin yontemiyle yuksek yuzey alanina sahip, glisin, tirozin ve glutamik asit aminoasitleri ile olusturulmus poliamid 6 polimer yuzeyler uretilmis ve liyofilize Bacillus subtilis E6-5 proteaz ve ticari proteaz enzimleri nanofibriller uzerinde immobilize edilmistir. Enzimlerin yeniden kullanilabilirligi arastirildi. Enzimlerin immobilizasyon verimlilikleri yaklasik olarak % 50-55 arasindaydi. Liyofilize Bacillus proteazi ile yapilan calismalarda glutaraldehitle aktiflestirilmis PA6 nanolifler ve glutaraldehitle aktiflestirilmeyen PA6 nanoliflerde glutamik asit aminoasidi varliginda immobilizasyonun daha basarili oldugu saptanmistir. Glutaraldehit ile aktiflestirilmemis ve aktiflestirilmis yuzeylerde immobilize edilen liyofilize proteaz enziminin 4 kez kullanimi olmasina ragmen, en iyi islevsel stabilite 2 kez kullanim ile elde edilmistir. Saf PA6/glutamik asit nanoliflerinde iki tekrarli kullanim sonucu enzimin immobilizasyon verimi % 38 olarak bulunmustur. Glutaraldehitle aktiflestirilmis PA6 nanoliflerde de PA6/glutamik asit nanolif yuzeyleri iki tekrarli kullanim sonucu enzimin immobilizasyon verimi % 65 olarak bulunmustur. Nanoliflerin glutaraldehitle aktiflestirmesi sonucu enzim immobilizasyon verimi iki kat arttirilmistir. Ticari proteaz ile yapilan calismalarda ise glutaraldehitle aktiflestirilmemis nanolif yuzeylerde enzimin 6 kez kullanimi olmasina ragmen en islevsel stabilite 3 tekrarli kullanimda elde edilmistir. En basarili immobilizasyon verimi PA6 nanoliflerde % 58 olarak bulunmustur. Glutaraldehitle aktiflestirilmis PA6 nanoliflerde de enzim 6 kez kullanim bulmus fakat islevsel stabilite 4 tekrarli kullanima kadar korunmustur.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72581788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SARS CoV-2 SPIKE GLYCOPROTEIN MUTATIONS AND CHANGES IN PROTEIN STRUCTURE SARS CoV-2刺突糖蛋白突变及蛋白结构改变

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Trakya University Journal of Natural Sciences Pub Date : 2020-02-16 DOI: 10.23902/trkjnat.774926

Ekrem Akbulut

{"title":"SARS CoV-2 SPIKE GLYCOPROTEIN MUTATIONS AND CHANGES IN PROTEIN STRUCTURE","authors":"Ekrem Akbulut","doi":"10.23902/trkjnat.774926","DOIUrl":"https://doi.org/10.23902/trkjnat.774926","url":null,"abstract":"Şiddetli akut solunum yolu sendromu koronavirusu-2 (SARS CoV-2) yuksek virulans etkiye sahip tek zincirli pozitif polariteli RNA virusudur. Spike (S) glikoprotein SARS CoV-2 virionunun en distaki bilesenidir ve anjiyotensin donusturucu enzim 2 (ACE2) reseptoru araciligi ile virusun hucreye girisinde onemlidir. ACE2, vazokonstriktor anjiyotensin 2'yi vazodilator anjiyotensin 1-7'ye donusturerek insanda kan basincinin duzenlenmesinde onemli roller ustlenir. Bu calismada, Asya izolatlarindaki mutasyonlarin S glikoprotein yapisinda neden olabilecegi degisiklikler analiz edilmis ve ilac ve asi hedefleme calismalarina katkida bulunmak uzere modellenmistir. Genom, proteom ve mutasyon analizleri biyoinformatik araclari (MAFFT, MegaX, PSIPRED, MolProbity, PyMoL) kullanilarak yapildi. Protein modellemesi ProMod3 kullanilarak yapildi. S glikoproteinde 26 mutasyon tespit edilmistir. Bu mutasyonlarin S glikoproteininin genel topolojik ve konformasyonel yapisinda ortaya cikardigi degisiklikler, SARS CoV-2’nin virulans ozelliklerini etkileyebilir. Mutasyonlarin reseptor baglanma bolgesini (RBB) kapali formasyondan acik formasyon benzeri bir yapiya donusturdugu belirlenmistir. RBB'de mutasyondan sonra meydana gelen konformasyonel degisimin reseptor afinitesinde bir artisa neden olabilecegi dusunulmektedir. Bu bulgular hastaligin onlenmesi ve SARS CoV-2 ilac ve asi gelistirme calismalari icin faydali olabilir.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2020-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77357118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2