Tissue Engineering Part A最新文献

{"title":"Generation of Monkey Induced Pluripotent Stem Cell-Derived Cartilage Lacking Major Histocompatibility Complex Class I Molecules on the Cell Surface.","authors":"Yuki Okutani, Kengo Abe, Akihiro Yamashita, Miho Morioka, Shuichi Matsuda, Noriyuki Tsumaki","doi":"10.1089/ten.TEA.2021.0053","DOIUrl":"https://doi.org/10.1089/ten.TEA.2021.0053","url":null,"abstract":"<p><p>Due to the poor capacity for articular cartilage to regenerate, its damage tends to result in progressively degenerating conditions such as osteoarthritis. To repair the damage, the transplantation of allogeneic human induced pluripotent stem cell (iPSC)-derived cartilage is being considered. However, although allogeneic cartilage transplantation is effective, immunological reactions can occur. One hypothetical solution is to delete the expression of major histocompatibility complex (MHC) class I molecules to reduce the immunological reactions. For this purpose, we deleted the β2 microglobulin (B2M) gene in a cynomolgus monkey (crab-eating monkey [Macaca fascicularis]) iPS cells (cyiPSCs) to obtain <i>B2M<sup>-/-</sup></i> cyiPSCs using the CRISPR/Cas9 system. Western blot analysis confirmed <i>B2M<sup>-/-</sup></i> cyiPSCs lacked B2M protein, which is necessary for MHC class I molecules to be transported to and expressed on the cell surface by forming multimers with B2M. Flow cytometry analysis revealed no <i>B2M<sup>-/-</sup></i> cyiPSCs expressed MHC class I molecules on their surface. The transplantation of <i>B2M<sup>-/-</sup></i> cyiPSCs in immunodeficient mice resulted in teratoma that contained cartilage, indicating that the lack of MHC class I molecules on the cell surface affects neither the pluripotency nor the chondrogenic differentiation capacity of cyiPSCs. By modifying the chondrogenic differentiation protocol for human iPSCs, we succeeded at differentiating <i>B2M<sup>+/+</sup></i> and <i>B2M<sup>-/-</sup></i> cyiPSCs toward chondrocytes followed by cartilage formation <i>in vitro</i>, as indicated by histological analysis showing that <i>B2M<sup>+/+</sup></i> and <i>B2M<sup>-/-</sup></i> cyiPSC-derived cartilage were positively stained with safranin O and expressed type II collagen. Flow cytometry analysis confirmed that MHC class I molecules were not expressed on the cell surface of <i>B2M<sup>-/-</sup></i> chondrocytes isolated from <i>B2M<sup>-/-</sup></i> cyiPSC-derived cartilage. An <i>in vitro</i> mixed lymphocyte reaction assay showed that neither <i>B2M<sup>+/+</sup></i> nor <i>B2M<sup>-/-</sup></i> cyiPSC-derived cartilage cells stimulated the proliferation of allogeneic peripheral blood mononuclear cells. On the contrary, osteochondral defects in monkey knee joints that received allogeneic transplantations of cyiPSC-derived cartilage showed an accumulation of leukocytes with more natural killer cells around <i>B2M<sup>-/-</sup></i> cyiPSC-derived cartilage than <i>B2M<sup>+/+</sup></i> cartilage, suggesting complex mechanisms in the immune reaction of allogeneic cartilage transplanted in osteochondral defects <i>in vivo</i>. Impact statement The transplantation of allogeneic induced pluripotent stem cell (iPSC)-derived cartilage is expected to treat articular cartilage damage, although the effects of major histocompatibility complex (MHC) in immunological reactions have not been well studied. We succeeded at creat","PeriodicalId":23133,"journal":{"name":"Tissue Engineering Part A","volume":" ","pages":"94-106"},"PeriodicalIF":4.1,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8792499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39117186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioengineering of brain organoids: Advancements and challenges","authors":"Renjitha Gopurappilly, R. Pal","doi":"10.1016/b978-0-12-824064-9.00002-2","DOIUrl":"https://doi.org/10.1016/b978-0-12-824064-9.00002-2","url":null,"abstract":"","PeriodicalId":23133,"journal":{"name":"Tissue Engineering Part A","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53910017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Translational tissue engineering","authors":"A. Nair, S. Nadine, S. Ahadian, J. Mano, N. Tanideh, Finosh G. Thankam","doi":"10.1016/b978-0-12-824064-9.00023-x","DOIUrl":"https://doi.org/10.1016/b978-0-12-824064-9.00023-x","url":null,"abstract":"","PeriodicalId":23133,"journal":{"name":"Tissue Engineering Part A","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53911143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic wounds and tissue engineering: Prospective and promise","authors":"Jahnavi Mudigonda, Smitha Chenicheri, R. Ramachandran","doi":"10.1016/b978-0-12-824064-9.00001-0","DOIUrl":"https://doi.org/10.1016/b978-0-12-824064-9.00001-0","url":null,"abstract":"","PeriodicalId":23133,"journal":{"name":"Tissue Engineering Part A","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53910012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D modeling of the lung in health and disease","authors":"R. Surolia, K. Dsouza, V. Antony","doi":"10.1016/b978-0-12-824064-9.00016-2","DOIUrl":"https://doi.org/10.1016/b978-0-12-824064-9.00016-2","url":null,"abstract":"","PeriodicalId":23133,"journal":{"name":"Tissue Engineering Part A","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53910448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Introduction","authors":"Jeong Yoon","doi":"10.1007/978-3-030-83696-2_1","DOIUrl":"https://doi.org/10.1007/978-3-030-83696-2_1","url":null,"abstract":"","PeriodicalId":23133,"journal":{"name":"Tissue Engineering Part A","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44218041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contact Guidance and Cell Patterning","authors":"Jeong‐Yeol Yoon","doi":"10.1007/978-3-030-83696-2_8","DOIUrl":"https://doi.org/10.1007/978-3-030-83696-2_8","url":null,"abstract":"","PeriodicalId":23133,"journal":{"name":"Tissue Engineering Part A","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46054496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem Cells","authors":"Jeong Yoon","doi":"10.1007/978-3-030-83696-2_5","DOIUrl":"https://doi.org/10.1007/978-3-030-83696-2_5","url":null,"abstract":"","PeriodicalId":23133,"journal":{"name":"Tissue Engineering Part A","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41987199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue-Engineered Skin Transplant","authors":"Jeong‐Yeol Yoon","doi":"10.1007/978-3-030-83696-2_12","DOIUrl":"https://doi.org/10.1007/978-3-030-83696-2_12","url":null,"abstract":"","PeriodicalId":23133,"journal":{"name":"Tissue Engineering Part A","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42001280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organ-on-a-Chip","authors":"Jeong-Yeol Yoon","doi":"10.1007/978-3-030-83696-2_11","DOIUrl":"https://doi.org/10.1007/978-3-030-83696-2_11","url":null,"abstract":"","PeriodicalId":23133,"journal":{"name":"Tissue Engineering Part A","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46773092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}