Tissue Engineering Part A最新文献

{"title":"Antithrombotic revascularization strategy of bioengineered liver using a biomimetic polymer.","authors":"Hiroshi Horie,Yu Oshima,Ken Fukumitsu,Kentaro Iwaki,Fumiaki Munekage,Kenta Makino,Satoshi Wakama,Takashi Ito,Katsuhiro Tomofuji,Saotshi Ogiso,Elena Yukie Uebayashi,Takamichi Ishii,Kazuhiko Ishihara,Etsuro Hatano","doi":"10.1089/ten.tea.2024.0131","DOIUrl":"https://doi.org/10.1089/ten.tea.2024.0131","url":null,"abstract":"A bioengineered liver has the potential to save patients with end-stage liver disease, and a three-dimensional decellularized scaffold is a promising approach for practical use. The main challenge in bioengineered liver transplantation is thrombogenicity during blood perfusion. We aimed to apply a novel antithrombotic polymer to revascularize liver scaffolds and evaluate the thrombogenicity and biosafety of the polymer-treated scaffolds. A biomimetic polymer, 2-metacryloyloxyethyl phosphorylcholine (MPC) was prepared for modification of the extracellular matrix (ECM) in liver scaffolds. The polymer was injected into the rat liver scaffolds' portal vein (PV) and could extensively react to the vessel walls. In an ex-vivo blood perfusion experiment, we demonstrated significantly less platelet deposition in the polymer-treated scaffolds than non-treated or re-endothelialized scaffolds with human umbilical endothelial cells (HUVECs). In the heterotopic transplantation model, liver volume was better maintained in the polymer-treated groups and platelet deposition was suppressed in these groups. Additionally, the polymer-treated liver scaffolds maintained the metabolic function of the recellularized rat primary hepatocytes during perfusion culture. The MPC polymer treatment efficiently suppressed thrombus formation during blood perfusion in liver scaffolds and maintained the function of recellularized hepatocytes. Revascularizing liver scaffolds using this polymer is a promising approach for bioengineered liver transplantation.","PeriodicalId":23133,"journal":{"name":"Tissue Engineering Part A","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142263934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of Commercially Available Extracellular Matrix Soft Tissue Bioscaffolds.","authors":"Tarek Kollmetz,Fernanda Castillo-Alcala,Robert W F Veale,Navid Taghavi,Vonne M van Heeswijk,Maarten Persenaire,Barnaby C H May,Sandi Grainne Dempsey","doi":"10.1089/ten.tea.2024.0076","DOIUrl":"https://doi.org/10.1089/ten.tea.2024.0076","url":null,"abstract":"Decellularized extracellular matrix (dECM) products are widely established for soft tissue repair, reconstruction and reinforcement. These regenerative biomaterials mimic native tissue ECM with respect to structure and biology and are produced from a range of tissue sources and species. Optimal source tissue processing requires a balance between removal of cellular material and the preservation of structural and biological properties of tissue ECM. Despite the wide-spread clinical use of dECM products there is a lack of comparative information on these products Structurally, some dECM products showed a well-preserved collagen architecture with a broad porosity distribution, while others showed a significantly altered structure compared with native tissue. Decellularization varied across the products. Some materials surveyed (OFMm, PPN, PPC, OFMo, UBM, SISz, ADM, PADM and BADM) were essentially devoid of nuclear bodies (mean count of <5 cells per high powered field (HPF)), whereas others (SISu and SISb) demonstrated an abundance of nuclear bodies (>50 cells per HPF). Pathology assessment of the products demonstrated that OFMm, OFMo and PADM had the highest qualitative assessment score for collagen fiber orientation and arrangement, matrix porosity, decellularization efficiency, and residual vascular channels scoring 10.5±0.8, 12.8±1.0, and 9.7±0.7 out of a maximum total score of 16, respectively This analysis of commercially available dECM products in terms of their structure and cellularity includes 12 different commercial materials The findings highlight the variability of the products in terms of matrix structure and the efficacy of decellularization.","PeriodicalId":23133,"journal":{"name":"Tissue Engineering Part A","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142263937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofabricated adipose-derived mesenchymal cell sheets recover cryo-injured kidneys in rats.","authors":"Ryo Kitahara,Tetsuya Imamura,Takahisa Domen,Yuki Matsumoto,Yoshihiro Inoue,Noriyuki Ogawa,Tetsuichi Saito,Manabu Ueno,Tomonori Minagawa,Teruyuki Ogawa,Osamu Ishizuka","doi":"10.1089/ten.tea.2024.0164","DOIUrl":"https://doi.org/10.1089/ten.tea.2024.0164","url":null,"abstract":"This study aimed to develop a treatment for chronic kidney disease (CKD) by investigating whether transplantation of biofabricated adipose-derived mesenchymal cell (AMC) sheets could improve renal tissue and function. Thirty-nine 10-week-old male Sprague-Dawley rats underwent the harvesting of adipose tissues and right nephrectomy. AMCs that were collected from adipose tissues were labeled and cultured on temperature-responsive dishes, and applied to a gelatin hydrogel sheet. Subsequently, two identical AMC-gelatin sheets were attached together to biofabricate a bilayered AMC-gelatin sheet. Further, 3 weeks after nephrectomy, the renal artery and vein of the left kidney were clamped, and the kidney was sprayed with liquid nitrogen for 60 seconds. The biofabricated AMC sheet was autologously transplanted into the renal capsule of the cryo-injured region (n = 14). Control rats were given acellular sheet (n = 25). One day before and four weeks after transplantation, blood and 24-hour urinary specimens were collected. Histological analysis of the experimental kidneys was performed four weeks after transplantation. Four weeks after transplantation, in the acellular control-transplanted rats, creatinine clearance levels tended to increase, while serum creatinine levels significantly increased. However, in the biofabricated AMC sheet-transplanted rats, creatinine clearance levels significantly increased, and serum creatinine levels remained unchanged and were significantly lower than that of the control rats. The ratio of damaged to undamaged renal tubules in the AMC sheet-transplanted rats was lower than that in the control rats. In addition, the occupancy rate of fibrotic areas in the renal cortex under the AMC sheet-transplanted regions was significantly lower than that in the control regions. After transplantation, while the expressions of transforming growth factor-beta 1 and hypoxia-inducible factor-1 alpha were observed in both the control- and AMC sheet-transplanted regions, these expressions tended to be lower in the AMC sheet-transplanted rats than in the control rats. The labeled transplanted AMCs were detected in the transplanted regions, with some of them also showing positive staining for the vascular endothelial growth factor antibody. In conclusion, the biofabricated AMC sheets improved renal functions by ameliorating renal tubule disorders and renal fibrosis. Therefore, biofabricated AMC sheets would serve as a potential treatment for CKD.","PeriodicalId":23133,"journal":{"name":"Tissue Engineering Part A","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142263933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organotypic 3D cellular models mimicking the epithelio-ectomesenchymal bi-layer during odontogenesis.","authors":"Fadi Jerbaka,Varvara Gribova,Tristan Rey,Soufian El-Faloussi,Marzena Kawczynski,Naji Kharouf,Yann Hérault,Youri Arntz,Agnès Bloch-Zupan,Isaac Maximiliano Maximiliano Bugueno Valdebenito","doi":"10.1089/ten.tea.2024.0118","DOIUrl":"https://doi.org/10.1089/ten.tea.2024.0118","url":null,"abstract":"Odontogenesis, the intricate process of tooth development, involves complex interactions between oral ectoderm epithelial cells and ectomesenchymal cells derived from the cephalic neural crest, regulated by major signaling pathways. Dental developmental anomalies provide valuable insights for clinical diagnosis of rare diseases. More than 30% of rare diseases patients who undergo molecular analysis suffer from diagnostic errancy. In the search for up-to-date technologies and methods to study the pathophysiology of new candidate genetic variants, causing tooth mineralized tissues anomalies, we have developed an original model of tooth organoids with human or mouse cell lines of ameloblast-like cells and odontoblasts derived from the pulp. This in vitro 3D cellular model reproducing the two main compartments of the bell stage of tooth development between ameloblasts and odontoblasts, specific to enamel and dentin morphogenesis, respectively, mimics the epithelio-mesenchymal interactions during the dental bell stage of tooth morphogenesis and will facilitate the study of enamel and dentin genetic anomalies, allowing the functional validation of newly identified mutations (variants of uncertain significance -VUS- or new candidate genes).","PeriodicalId":23133,"journal":{"name":"Tissue Engineering Part A","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142263935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rosalind Franklin Society Proudly Announces the 2023 Award Recipient for Tissue Engineering Part A.","authors":"Mary Beth B Monroe","doi":"10.1089/ten.tea.2024.25467.rfs2023","DOIUrl":"https://doi.org/10.1089/ten.tea.2024.25467.rfs2023","url":null,"abstract":"","PeriodicalId":23133,"journal":{"name":"Tissue Engineering Part A","volume":"31 1","pages":"511"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142263936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling viral infection with tissue engineering: COVID-19 and the next outbreaks","authors":"A. Tatara","doi":"10.1016/B978-0-12-824064-9.00015-0","DOIUrl":"https://doi.org/10.1016/B978-0-12-824064-9.00015-0","url":null,"abstract":"","PeriodicalId":23133,"journal":{"name":"Tissue Engineering Part A","volume":"1 1","pages":"647 - 667"},"PeriodicalIF":0.0,"publicationDate":"2022-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42521797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Future of nanotechnology in tissue engineering","authors":"V. Vijayan, Gerardo Hernandez-Moreno, V. Thomas","doi":"10.1016/b978-0-12-824064-9.00003-4","DOIUrl":"https://doi.org/10.1016/b978-0-12-824064-9.00003-4","url":null,"abstract":"","PeriodicalId":23133,"journal":{"name":"Tissue Engineering Part A","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53910022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dental pulp tissue regeneration","authors":"I. J. de Souza Araújo, E. Münchow, S. Tootla, M. Bottino","doi":"10.1016/b978-0-12-824064-9.00005-8","DOIUrl":"https://doi.org/10.1016/b978-0-12-824064-9.00005-8","url":null,"abstract":"","PeriodicalId":23133,"journal":{"name":"Tissue Engineering Part A","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53910034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue regeneration: Fetal to adult transition","authors":"Ajoy Aloysius","doi":"10.1016/b978-0-12-824064-9.00020-4","DOIUrl":"https://doi.org/10.1016/b978-0-12-824064-9.00020-4","url":null,"abstract":"","PeriodicalId":23133,"journal":{"name":"Tissue Engineering Part A","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53910657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosensors in tissue engineering","authors":"Yubin Zhou, Huizhi Chen, Lianxian Guo, Jianqiang Liu, Hui Zhou, Liyan Wang, H. S. Nanda, Xinsheng Peng","doi":"10.1016/b978-0-12-824064-9.00026-5","DOIUrl":"https://doi.org/10.1016/b978-0-12-824064-9.00026-5","url":null,"abstract":"","PeriodicalId":23133,"journal":{"name":"Tissue Engineering Part A","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53910710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}