The Open Virology Journal最新文献_第8页

Virus-Like Particles Harboring CCL19, IL-2 and HPV16 E7 Elicit Protective T Cell Responses in HLA-A2 Transgenic Mice. 携带CCL19、IL-2和hpv16e7的病毒样颗粒在HLA-A2转基因小鼠中引发保护性T细胞反应

The Open Virology Journal Pub Date : 2012-01-01 Epub Date: 2012-12-28 DOI: 10.2174/1874357901206010270

Victoria Juarez, H Amalia Pasolli, Andrea Hellwig, Natalio Garbi, Angel Cid Arregui

{"title":"Virus-Like Particles Harboring CCL19, IL-2 and HPV16 E7 Elicit Protective T Cell Responses in HLA-A2 Transgenic Mice.","authors":"Victoria Juarez, H Amalia Pasolli, Andrea Hellwig, Natalio Garbi, Angel Cid Arregui","doi":"10.2174/1874357901206010270","DOIUrl":"https://doi.org/10.2174/1874357901206010270","url":null,"abstract":"Infection by high-risk genotypes of human papillomaviruses (HR-HPVs) is the cause of cancer of the uterine cervix. Although prophylactic vaccines directed against the two most prevalent HR-HPV types (HPV16 and 18) have been commercialized recently, there is a need for effective therapeutic vaccines against HR-HPVs. We have tested in mice a chimeric protein composed of the hepatitis B small surface antigen (HBsAg(S)) flanked at its N-terminus by chemokine CC ligand 19/macrophage inflammatory protein-3β (CCL19/MIP-3β), and at the C-terminus by interleukin 2 (IL-2) and an artificial HPV16 E7 polytope. This protein is assembled into nanoparticles and both CCL19 and IL-2 conserve their functionality. HLA-A2 (AAD) transgenic mice immunized with a plasmid encoding this protein mounted specific T cell responses against E7 without the need of an adjuvant. Furthermore, vaccination prevented the development of tumors after implantation of the E6/E7-expressing TC-1/A2 tumor cell line. Our results suggest that vaccines based on HBsAg(S) nanoparticles carrying short E7 epitopes and immune-stimulatory domains might be of therapeutic value in the treatment of patients suffering from cervical pre-cancer or cancer lesions caused by HR-HPVs.","PeriodicalId":23111,"journal":{"name":"The Open Virology Journal","volume":"6 ","pages":"270-6"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2174/1874357901206010270","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31179844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

The HPV E2-Host Protein-Protein Interactions: A Complex Hijacking of the Cellular Network. HPV e2 -宿主蛋白-蛋白相互作用:细胞网络的复杂劫持。

The Open Virology Journal Pub Date : 2012-01-01 Epub Date: 2012-12-28 DOI: 10.2174/1874357901206010173

Mandy Muller, Caroline Demeret

{"title":"The HPV E2-Host Protein-Protein Interactions: A Complex Hijacking of the Cellular Network.","authors":"Mandy Muller, Caroline Demeret","doi":"10.2174/1874357901206010173","DOIUrl":"https://doi.org/10.2174/1874357901206010173","url":null,"abstract":"Over 100 genotypes of human papillomaviruses (HPVs) have been identified as being responsible for unapparent infections or for lesions ranging from benign skin or genital warts to cancer. The pathogenesis of HPV results from complex relationships between viral and host factors, driven in particular by the interplay between the host proteome and the early viral proteins. The E2 protein regulates the transcription, the replication as well as the mitotic segregation of the viral genome through the recruitment of host cell factors to the HPV regulatory region. It is thereby a pivotal factor for the productive viral life cycle and for viral persistence, a major risk factor for cancer development. In addition, the E2 proteins have been shown to engage numerous interactions through which they play important roles in modulating the host cell. Such E2 activities are probably contributing to create cell conditions appropriate for the successive stages of the viral life cycle, and some of these activities have been demonstrated only for the oncogenic high-risk HPV. The recent mapping of E2-host protein-protein interactions with 12 genotypes representative of HPV diversity has shed some light on the large complexity of the host cell hijacking and on its diversity according to viral genotypes. This article reviews the functions of E2 as they emerge from the E2/host proteome interplay, taking into account the large-scale comparative interactomic study.","PeriodicalId":23111,"journal":{"name":"The Open Virology Journal","volume":"6 ","pages":"173-89"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2174/1874357901206010173","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31181440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA interference for the treatment of papillomavirus disease. RNA干扰治疗乳头瘤病毒病。

The Open Virology Journal Pub Date : 2012-01-01 Epub Date: 2012-12-28 DOI: 10.2174/1874357901206010204

Richa Singhania, Norliana Khairuddin, Daniel Clarke, Nigel Aj McMillan

引用次数: 0

Human papillomavirus infections and cancer stem cells of tumors from the uterine cervix. 人乳头瘤病毒感染与子宫颈肿瘤的癌干细胞。

The Open Virology Journal Pub Date : 2012-01-01 Epub Date: 2012-12-28 DOI: 10.2174/1874357901206010232

Jacqueline López, Graciela Ruíz, Jorge Organista-Nava, Patricio Gariglio, Alejandro García-Carrancá

{"title":"Human papillomavirus infections and cancer stem cells of tumors from the uterine cervix.","authors":"Jacqueline López, Graciela Ruíz, Jorge Organista-Nava, Patricio Gariglio, Alejandro García-Carrancá","doi":"10.2174/1874357901206010232","DOIUrl":"10.2174/1874357901206010232","url":null,"abstract":"Different rate of development of productive infections (as low grade cervical intraepithelial neoplasias), or high grade lesions and cervical malignant tumors associated with infections of the Transformation zone (TZ) by High-Risk Human Papillomavirus (HR-HPV), could suggest that different epithelial host target cells could exist. If there is more than one target cell, their differential infection by HR-HPV may play a central role in the development of cervical cancer. Recently, the concept that cancer might arise from a rare population of cells with stem cell-like properties has received support in several solid tumors, including cervical cancer (CC). According to the cancer stem cell (CSC) hypothesis, CC can now be considered a disease in which stem cells of the TZ are converted to cervical cancer stem cells by the interplay between HR-HPV viral oncogenes and cellular alterations that are thought to be finally responsible for tumor initiation and maintenance. Current studies of CSC could provide novel insights regarding tumor initiation and progression, their relation with viral proteins and interplay with the tumor micro-environment. This review will focus on the biology of cervical cancer stem cells, which might contribute to our understanding of the mechanisms responsible for cervical tumor development.","PeriodicalId":23111,"journal":{"name":"The Open Virology Journal","volume":"6 ","pages":"232-40"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/97/01/TOVJ-6-232.PMC3547319.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31181445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laboratory detection of respiratory viruses by automated techniques. 利用自动化技术对呼吸道病毒进行实验室检测。

The Open Virology Journal Pub Date : 2012-01-01 Epub Date: 2012-11-30 DOI: 10.2174/1874357901206010151

Mercedes Pérez-Ruiz, Irene Pedrosa-Corral, Sara Sanbonmatsu-Gámez, María Navarro-Marí

{"title":"Laboratory detection of respiratory viruses by automated techniques.","authors":"Mercedes Pérez-Ruiz, Irene Pedrosa-Corral, Sara Sanbonmatsu-Gámez, María Navarro-Marí","doi":"10.2174/1874357901206010151","DOIUrl":"10.2174/1874357901206010151","url":null,"abstract":"Advances in clinical virology for detecting respiratory viruses have been focused on nucleic acids amplification techniques, which have converted in the reference method for the diagnosis of acute respiratory infections of viral aetiology. Improvements of current commercial molecular assays to reduce hands-on-time rely on two strategies, a stepwise automation (semi-automation) and the complete automation of the whole procedure. Contributions to the former strategy have been the use of automated nucleic acids extractors, multiplex PCR, real-time PCR and/or DNA arrays for detection of amplicons. Commercial fully-automated molecular systems are now available for the detection of respiratory viruses. Some of them could convert in point-of-care methods substituting antigen tests for detection of respiratory syncytial virus and influenza A and B viruses. This article describes laboratory methods for detection of respiratory viruses. A cost-effective and rational diagnostic algorithm is proposed, considering technical aspects of the available assays, infrastructure possibilities of each laboratory and clinic-epidemiologic factors of the infection.","PeriodicalId":23111,"journal":{"name":"The Open Virology Journal","volume":"6 ","pages":"151-9"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/09/61/TOVJ-6-151.PMC3522051.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31127017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving Clinical Laboratory Efficiency: Introduction of Systems for the Diagnosis and Monitoring of HIV Infection. 提高临床实验室效率:引进HIV感染诊断和监测系统。

The Open Virology Journal Pub Date : 2012-01-01 Epub Date: 2012-11-30 DOI: 10.2174/1874357901206010135

Marta Alvarez, Natalia Chueca, Vicente Guillot, María Del Carmen Bernal, Federico García

{"title":"Improving Clinical Laboratory Efficiency: Introduction of Systems for the Diagnosis and Monitoring of HIV Infection.","authors":"Marta Alvarez, Natalia Chueca, Vicente Guillot, María Del Carmen Bernal, Federico García","doi":"10.2174/1874357901206010135","DOIUrl":"https://doi.org/10.2174/1874357901206010135","url":null,"abstract":"Since the first tests for identifying individuals with suspected human immunodeficiency virus (HIV) infection were introduced in the mid-1980s, diagnostic virology testing has greatly evolved. The technological advances, automating in the laboratories and the advances in molecular biology techniques have helped introduce invaluable laboratory methods for managing HIV patients. Tests for diagnosis, specially for screening HIV antibodies, are now fully automated; in the same way, tests for monitoring HIV viral load (HIV RNA copies/ml of plasma), which is used for monitoring infection and response to antiretroviral treatment, are also fully automated; however, resistance testing, tropism determination and minor variant detection, which are used to make decisions for changing antiretroviral treatment regimens in patients failing therapy, still remain highly laborious and time consuming. This chapter will review the main aspects relating to the automating of the methods available for laboratory diagnosis as well as for monitoring of the HIV infection and determination of resistance to antiretrovirals and viral tropism.","PeriodicalId":23111,"journal":{"name":"The Open Virology Journal","volume":"6 ","pages":"135-43"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2174/1874357901206010135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31144494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Immunotherapy of human papilloma virus induced disease. 人乳头瘤病毒诱导疾病的免疫治疗。

The Open Virology Journal Pub Date : 2012-01-01 Epub Date: 2012-12-28 DOI: 10.2174/1874357901206010257

Sjoerd H van der Burg

引用次数: 9

Genome Stability of Pandemic Influenza A (H1N1) 2009 Based on Analysis of Hemagglutinin and Neuraminidase Genes. 基于血凝素和神经氨酸酶基因分析的2009年甲型H1N1流感基因组稳定性

The Open Virology Journal Pub Date : 2012-01-01 Epub Date: 2012-04-26 DOI: 10.2174/1874357901206010059

Emilio E Espínola

引用次数: 10

The E89K Mutation in the Matrix Protein of the Measles Virus Affects In Vitro Cell Death and Virus Replication Efficiency in Human PBMC. 麻疹病毒基质蛋白E89K突变影响人PBMC体外细胞死亡和病毒复制效率

The Open Virology Journal Pub Date : 2012-01-01 Epub Date: 2012-06-08 DOI: 10.2174/1874357901206010068

Jianbao Dong, Wei Zhu, Akatsuki Saito, Yoshitaka Goto, Hiroyuki Iwata, Takeshi Haga

引用次数: 2

Development and Field Testing of a Real-Time PCR Assay for Caprine Arthritis-Encephalitis-Virus (CAEV). 山羊关节炎-脑炎病毒(CAEV)实时荧光定量PCR检测方法的建立及现场试验

The Open Virology Journal Pub Date : 2012-01-01 Epub Date: 2011-07-27 DOI: 10.2174/1874357901206010082

Giovanni Brajon, Daniela Mandas, Manuele Liciardi, Flavia Taccori, Mauro Meloni, Franco Corrias, Caterina Montaldo, Ferdinando Coghe, Cristina Casciari, Monica Giammarioli, Germano Orrù

{"title":"Development and Field Testing of a Real-Time PCR Assay for Caprine Arthritis-Encephalitis-Virus (CAEV).","authors":"Giovanni Brajon, Daniela Mandas, Manuele Liciardi, Flavia Taccori, Mauro Meloni, Franco Corrias, Caterina Montaldo, Ferdinando Coghe, Cristina Casciari, Monica Giammarioli, Germano Orrù","doi":"10.2174/1874357901206010082","DOIUrl":"https://doi.org/10.2174/1874357901206010082","url":null,"abstract":"Caprine arthritis/encephalitis (CAE) of goats and occasionally sheep are persistent virus infections caused by a lentivirus (CAEV). This viral infection results in arthritis in adult animals and encephalitis in kids. Prognosis for the encephalitic form is normally poor, with substantial economic loss for the farm. In this context an early/fast laboratory diagnosis for CAEV infection could be useful for effective prophylactic action. In this work we performed a quantitative real time PCR designed on the CAEV env gene to detect/quantify in goat/sheep samples, viral RNA or proviral DNA forms of CAEV. This procedure was validated in 15 sheep, experimentally infected with CAEV or with a highly correlated lentivirus (visna maedi, MVV); in addition, a total of 37 clinical goat specimens recruited in CAEV positive herds were analyzed and compared using serological analysis (Elisa and AGID). All samples infected with MVV resulted negative. In sheep experimentally infected with CAEV, proviral DNA was detectable 15 days post infection, whereas the serological methods revealed an indicative positivity after 40-60 days.This method showed a sensitivity of 10(2) env fragments/PCR) with a linear dynamic range of quantitation from 10(3) to 10(7)env fragments/PCR; the R2 correlation coefficient was 0.98. All subjects with a clinical diagnosis for Caprine Arthritis-Encephalitis (CAE) resulted CAEV DNA positive.","PeriodicalId":23111,"journal":{"name":"The Open Virology Journal","volume":"6 ","pages":"82-90"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2174/1874357901206010082","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30830276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18