The Open Virology Journal最新文献_第10页

Strategies for the successful implementation of viral laboratory automation. 成功实施病毒实验室自动化的策略。

The Open Virology Journal Pub Date : 2012-01-01 Epub Date: 2012-11-30 DOI: 10.2174/1874357901206010115

Cristóbal Avivar

{"title":"Strategies for the successful implementation of viral laboratory automation.","authors":"Cristóbal Avivar","doi":"10.2174/1874357901206010115","DOIUrl":"https://doi.org/10.2174/1874357901206010115","url":null,"abstract":"It has been estimated that more than 70% of all medical activity is directly related to information providing analytical data. Substantial technological advances have taken place recently, which have allowed a previously unimagined number of analytical samples to be processed while offering high quality results. Concurrently, yet more new diagnostic determinations have been introduced - all of which has led to a significant increase in the prescription of analytical parameters. This increased workload has placed great pressure on the laboratory with respect to health costs. The present manager of the Clinical Laboratory (CL) has had to examine cost control as well as rationing - meaning that the CL's focus has not been strictly metrological, as if it were purely a system producing results, but instead has had to concentrate on its efficiency and efficacy. By applying re-engineering criteria, an emphasis has had to be placed on improved organisation and operating practice within the CL, focussing on the current criteria of the Integrated Management Areas where the technical and human resources are brought together. This re-engineering has been based on the concepts of consolidating and integrating the analytical platforms, while differentiating the production areas (CORE Laboratory) from the information areas. With these present concepts in mind, automation and virological treatment, along with serology in general, follow the same criteria as the rest of the operating methodology in the Clinical Laboratory.","PeriodicalId":23111,"journal":{"name":"The Open Virology Journal","volume":"6 ","pages":"115-21"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/50/1b/TOVJ-6-115.PMC3522095.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31129671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

The invisible enemy - how human papillomaviruses avoid recognition and clearance by the host immune system. 看不见的敌人——人类乳头瘤病毒如何避免被宿主免疫系统识别和清除。

The Open Virology Journal Pub Date : 2012-01-01 Epub Date: 2012-12-28 DOI: 10.2174/1874357901206010249

Agnieszka K Grabowska, Angelika B Riemer

引用次数: 0

Evidence and impact of human papillomavirus latency. 人乳头瘤病毒潜伏期的证据和影响。

The Open Virology Journal Pub Date : 2012-01-01 Epub Date: 2012-12-28 DOI: 10.2174/1874357901206010198

Patti E Gravitt

引用次数: 51

Loss of HPV16 E2 Protein Expression Without Disruption of the E2 ORF Correlates with Carcinogenic Progression. hpv16e2蛋白表达缺失而不破坏E2 ORF与致癌进展相关

The Open Virology Journal Pub Date : 2012-01-01 Epub Date: 2012-12-28 DOI: 10.2174/1874357901206010163

Yuezhen Xue, Diana Lim, Liang Zhi, Pingping He, Jean-Pierre Abastado, Françoise Thierry

{"title":"Loss of HPV16 E2 Protein Expression Without Disruption of the E2 ORF Correlates with Carcinogenic Progression.","authors":"Yuezhen Xue, Diana Lim, Liang Zhi, Pingping He, Jean-Pierre Abastado, Françoise Thierry","doi":"10.2174/1874357901206010163","DOIUrl":"https://doi.org/10.2174/1874357901206010163","url":null,"abstract":"Integration of the viral DNA in the cellular genome has been suggested to be critical in carcinogenic progression of HPV-associated cervical neoplasia. This event can be accompanied by disruption of the open reading frame (ORF) encoding the E2 repressor, thus leading to transcriptional up-regulation of the E6 and E7 viral oncogenes. At this stage, it is unclear whether disruption of the E2 ORF is mandatory for carcinogenic progression. We measured E2 RNA and protein expression in clinical samples of various grades of HPV16-associated cervical neoplasia and compared it with the status of the viral genome. RNA extracted from paraffin embedded tissues was hybridized to specific probes and quantified by the NanoString technology. Protein expression was appreciated by immunohistochemistry and the status of viral DNA was determined by in situ hybridization, all performed on serial sections of the same samples. E2 protein was found highly expressed in CIN1, CIN2 lesions where the HPV DNA was highly replicative, while it was decreased in more advanced grade lesions where replication is decreased or lost (CIN3 and SCC). In contrast, E2 transcripts could be elevated even in conditions of no or low expression of the protein, as found in the Caski cell line. Our data demonstrate that integration of the viral DNA in the cellular genome does not always lead to disruption of the E2 ORF and drastic reduction of E2 transcripts, while in contrast, expression of the E2 protein is always drastically reduced.","PeriodicalId":23111,"journal":{"name":"The Open Virology Journal","volume":"6 ","pages":"163-72"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3b/1d/TOVJ-6-163.PMC3547325.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31181439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

The microRNA Transcriptome of Human Cytomegalovirus (HCMV). 人巨细胞病毒(HCMV)的microRNA转录组。

The Open Virology Journal Pub Date : 2012-01-01 Epub Date: 2012-04-11 DOI: 10.2174/1874357901206010038

Mesfin K Meshesha, Isana Veksler-Lublinsky, Ofer Isakov, Irit Reichenstein, Noam Shomron, Klara Kedem, Michal Ziv-Ukelson, Zvi Bentwich, Yonat Shemer Avni

{"title":"The microRNA Transcriptome of Human Cytomegalovirus (HCMV).","authors":"Mesfin K Meshesha, Isana Veksler-Lublinsky, Ofer Isakov, Irit Reichenstein, Noam Shomron, Klara Kedem, Michal Ziv-Ukelson, Zvi Bentwich, Yonat Shemer Avni","doi":"10.2174/1874357901206010038","DOIUrl":"https://doi.org/10.2174/1874357901206010038","url":null,"abstract":"The purpose of the present study was to characterize the microRNA transcriptome (miRNAome) of the human cytomegalovirus (HCMV or HHV5). We used deep sequencing and real time PCR (qPCR) together with bioinformatics to analyze the pattern of small RNA expression in cells infected with low-passage isolates of HCMV as well as in plasma and amniotic fluid. We report here on the discovery of four new precursors and ten new miRNAs as well as eleven microRNA-offset-RNAs (moRs) that are all encoded by HCMV. About eighty percent of the total HCMV reads were perfectly mapped to HCMV miRNAs, strongly suggestive of their important biological role that in large remains still to be defined and characterized. Taken altogether, the results of this study demonstrate the power and usefulness of the combined bioinformatics/biological approach in discovering additional important members of HCMV- encoded small RNAs and can be applied to the study of other viruses as well.","PeriodicalId":23111,"journal":{"name":"The Open Virology Journal","volume":"6 ","pages":"38-48"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/67/ca/TOVJ-6-38.PMC3377890.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30704506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 45

Development and evaluation of an enzyme-linked immunosorbent assay for dengue capsid. 登革热衣壳酶联免疫吸附试验的建立与评价。

The Open Virology Journal Pub Date : 2012-01-01 Epub Date: 2012-03-29 DOI: 10.2174/1874357901206010029

Suganya Selvarajah, Udayan Chatterji, Richard Kuhn, Richard Kinney, Subhash G Vasudevan, Philippe Gallay

引用次数: 2

Application of molecular diagnostic techniques for viral testing. 分子诊断技术在病毒检测中的应用。

The Open Virology Journal Pub Date : 2012-01-01 Epub Date: 2012-11-30 DOI: 10.2174/1874357901206010104

Fernando Cobo

{"title":"Application of molecular diagnostic techniques for viral testing.","authors":"Fernando Cobo","doi":"10.2174/1874357901206010104","DOIUrl":"https://doi.org/10.2174/1874357901206010104","url":null,"abstract":"Nucleic acid amplification techniques are commonly used currently to diagnose viral diseases and manage patients with this kind of illnesses. These techniques have had a rapid but unconventional route of development during the last 30 years, with the discovery and introduction of several assays in clinical diagnosis. The increase in the number of commercially available methods has facilitated the use of this technology in the majority of laboratories worldwide. This technology has reduced the use of some other techniques such as viral culture based methods and serological assays in the clinical virology laboratory. Moreover, nucleic acid amplification techniques are now the methods of reference and also the most useful assays for the diagnosis in several diseases. The introduction of these techniques and their automation provides new opportunities for the clinical laboratory to affect patient care. The main objectives in performing nucleic acid tests in this field are to provide timely results useful for high-quality patient care at a reasonable cost, because rapid results are associated with improvements in patients care. The use of amplification techniques such as polymerase chain reaction, real-time polymerase chain reaction or nucleic acid sequence-based amplification for virus detection, genotyping and quantification have some advantages like high sensitivity and reproducibility, as well as a broad dynamic range. This review is an up-to-date of the main nucleic acid techniques and their clinical applications, and special challenges and opportunities that these techniques currently provide for the clinical virology laboratory.","PeriodicalId":23111,"journal":{"name":"The Open Virology Journal","volume":"6 ","pages":"104-14"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2174/1874357901206010104","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31129670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pilot Evaluation of RT-PCR/Electrospray Ionization Mass Spectrometry (PLEX-ID/Flu assay) on Influenza-Positive Specimens. RT-PCR/电喷雾质谱(PLEX-ID/流感测定)对流感阳性标本的初步评估

The Open Virology Journal Pub Date : 2012-01-01 Epub Date: 2012-05-09 DOI: 10.2174/1874357901206010064

Samuel Cordey, Yves Thomas, Patricia Suter, Laurent Kaiser

引用次数: 13

Characterization of Alternanthera mosaic virus and its Coat Protein. 互花花叶病毒及其外壳蛋白的鉴定。

The Open Virology Journal Pub Date : 2011-01-01 Epub Date: 2011-11-21 DOI: 10.2174/1874357901105010136

Anna A Mukhamedzhanova, Alexander A Smirnov, Marina V Arkhipenko, Peter A Ivanov, Sergey N Chirkov, Nina P Rodionova, Olga V Karpova, Joseph G Atabekov

引用次数: 15

In Vivo Interaction of the Hepatitis Delta Virus Small Antigen with the ELAV-Like Protein HuR. 丁型肝炎病毒小抗原与elav样蛋白HuR的体内相互作用

The Open Virology Journal Pub Date : 2011-01-01 Epub Date: 2011-03-24 DOI: 10.2174/1874357901105010012

Ana Casaca, Margarida Fardilha, Edgar da Cruz E Silva, Celso Cunha

引用次数: 3