The microRNA Transcriptome of Human Cytomegalovirus (HCMV).

The Open Virology Journal Pub Date : 2012-01-01 Epub Date: 2012-04-11 DOI:10.2174/1874357901206010038

Mesfin K Meshesha, Isana Veksler-Lublinsky, Ofer Isakov, Irit Reichenstein, Noam Shomron, Klara Kedem, Michal Ziv-Ukelson, Zvi Bentwich, Yonat Shemer Avni

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引用次数: 45

Abstract

The purpose of the present study was to characterize the microRNA transcriptome (miRNAome) of the human cytomegalovirus (HCMV or HHV5). We used deep sequencing and real time PCR (qPCR) together with bioinformatics to analyze the pattern of small RNA expression in cells infected with low-passage isolates of HCMV as well as in plasma and amniotic fluid. We report here on the discovery of four new precursors and ten new miRNAs as well as eleven microRNA-offset-RNAs (moRs) that are all encoded by HCMV. About eighty percent of the total HCMV reads were perfectly mapped to HCMV miRNAs, strongly suggestive of their important biological role that in large remains still to be defined and characterized. Taken altogether, the results of this study demonstrate the power and usefulness of the combined bioinformatics/biological approach in discovering additional important members of HCMV- encoded small RNAs and can be applied to the study of other viruses as well.

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人巨细胞病毒(HCMV)的microRNA转录组。

本研究的目的是表征人巨细胞病毒(HCMV或HHV5)的microRNA转录组(miRNAome)。我们采用深度测序和实时PCR (qPCR)技术，结合生物信息学分析了HCMV低传代分离株感染细胞、血浆和羊水中小RNA的表达模式。我们在此报告了四种新的前体和十个新的mirna以及十一个microRNA-offset-RNAs (moRs)的发现，它们都是由HCMV编码的。大约80%的HCMV读取完全映射到HCMV mirna，这强烈表明它们的重要生物学作用在很大程度上仍有待定义和表征。综上所述，本研究的结果证明了生物信息学/生物学结合方法在发现HCMV编码小rna的其他重要成员方面的力量和有效性，并且也可以应用于其他病毒的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Open Virology Journal

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