The Open Virology Journal最新文献_第5页

Molecular Detection of Human Cytomegalovirus (HCMV) Among Infants with Congenital Anomalies in Khartoum State, Sudan 苏丹喀土穆州先天性异常婴儿中人类巨细胞病毒(HCMV)的分子检测

The Open Virology Journal Pub Date : 2015-12-10 DOI: 10.2174/1874357901509010038

Maha G Ebrahim, Aisha S. Ali, Mohamed O. Mustafa, D. Musa, A. R. E. El Hussein, I. Elkhidir, K. Enan

{"title":"Molecular Detection of Human Cytomegalovirus (HCMV) Among Infants with Congenital Anomalies in Khartoum State, Sudan","authors":"Maha G Ebrahim, Aisha S. Ali, Mohamed O. Mustafa, D. Musa, A. R. E. El Hussein, I. Elkhidir, K. Enan","doi":"10.2174/1874357901509010038","DOIUrl":"https://doi.org/10.2174/1874357901509010038","url":null,"abstract":"Human Cytomegalovirus (HCMV) infection still represents the most common potentially serious viral complication in humans and is a major cause of congenital anomalies in infants. This study is aimed to detect HCMV in infants with congenital anomalies. Study subjects consisted of infants born with neural tube defect, hydrocephalus and microcephaly. Fifty serum specimens (20 males, 30 females) were collected from different hospitals in Khartoum State. The sera were investigated for cytomegalovirus specific immunoglobin M (IgM) antibodies using enzyme-linked immunosorbent assay (ELISA), and for Cytomegalovirus DNA using polymerase chain reaction (PCR). Out of the 50 sera tested, one patient’s (2%) sample showed HCMV IgM, but with no detectable DNA, other 4(8.2 %) sera were positive for HCMV DNA but with no detectable IgM. Various diagnostic techniques should be considered to evaluate HCMV disease and routine screening for HCMV should be introduced for pregnant women in this setting. It is vital to initiate further research work with many samples from different area to assess prevalence and characterize HCMV and evaluate its maternal health implications.","PeriodicalId":23111,"journal":{"name":"The Open Virology Journal","volume":"467 1","pages":"38 - 41"},"PeriodicalIF":0.0,"publicationDate":"2015-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86717627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Detection of Human Polyomavirus DNA Using the Genome Profiling Method. 用基因组谱法检测人多瘤病毒DNA。

The Open Virology Journal Pub Date : 2015-11-24 eCollection Date: 2015-01-01 DOI: 10.2174/1874357901509010029

Yuka Tanaka, Rieko Hirata, Kyohei Mashita, Stuart Mclean, Hiroshi Ikegaya

{"title":"Detection of Human Polyomavirus DNA Using the Genome Profiling Method.","authors":"Yuka Tanaka, Rieko Hirata, Kyohei Mashita, Stuart Mclean, Hiroshi Ikegaya","doi":"10.2174/1874357901509010029","DOIUrl":"https://doi.org/10.2174/1874357901509010029","url":null,"abstract":"Background: In the field of forensic medicine, it is very difficult to know prior to autopsy what kind of virus has infected a body.Objective: We assessed the potential of the genome profiling (GP) method, which was developed in the field of bioengineering, to identify viruses belonging to one species.Method: Two species in the same family, JC and BK viruses, were used in this study. Using plasmid samples, we compared the findings of molecular phylogenetic analysis using conventional genome sequencing with the results of cluster analysis using the random PCR-based GP method and discussed whether the GP method can be used to determine viral species.Results: It was possible to distinguish these two different viral species. In addition to this, in our trial we could also detect the JC virus from a clinical sample.Conclusion: This method does not require special reagent sets for each viral species. Though our findings are still in the trial period, the GP method may be a simple, easy, and economical tool to detect viral species in the near future.","PeriodicalId":23111,"journal":{"name":"The Open Virology Journal","volume":"9 ","pages":"29-37"},"PeriodicalIF":0.0,"publicationDate":"2015-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5396359/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34977813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Epstein- Barr Virus: Clinical and Epidemiological Revisits and Genetic Basis of Oncogenesis 爱泼斯坦-巴尔病毒:临床和流行病学回顾和肿瘤发生的遗传基础

The Open Virology Journal Pub Date : 2015-11-03 DOI: 10.2174/1874357901509010007

A. Ali, M. Al-Shraim, A. Al-Hakami, I. Jones

{"title":"Epstein- Barr Virus: Clinical and Epidemiological Revisits and Genetic Basis of Oncogenesis","authors":"A. Ali, M. Al-Shraim, A. Al-Hakami, I. Jones","doi":"10.2174/1874357901509010007","DOIUrl":"https://doi.org/10.2174/1874357901509010007","url":null,"abstract":"Epstein-Barr virus (EBV) is classified as a member in the order herpesvirales, family herpesviridae, subfamily gammaherpesvirinae and the genus lymphocytovirus. The virus is an exclusively human pathogen and thus also termed as human herpesvirus 4 (HHV4). It was the first oncogenic virus recognized and has been incriminated in the causation of tumors of both lymphatic and epithelial nature. It was reported in some previous studies that 95% of the population worldwide are serologically positive to the virus. Clinically, EBV primary infection is almost silent, persisting as a life-long asymptomatic latent infection in B cells although it may be responsible for a transient clinical syndrome called infectious mononucleosis. Following reactivation of the virus from latency due to immunocompromised status, EBV was found to be associated with several tumors. EBV linked to oncogenesis as detected in lymphoid tumors such as Burkitt's lymphoma (BL), Hodgkin's disease (HD), post-transplant lymphoproliferative disorders (PTLD) and T-cell lymphomas (e.g. Peripheral T-cell lymphomas; PTCL and Anaplastic large cell lymphomas; ALCL). It is also linked to epithelial tumors such as nasopharyngeal carcinoma (NPC), gastric carcinomas and oral hairy leukoplakia (OHL). In vitro, EBV many studies have demonstrated its ability to transform B cells into lymphoblastoid cell lines (LCLs). Despite these malignancies showing different clinical and epidemiological patterns when studied, genetic studies have suggested that these EBV- associated transformations were characterized generally by low level of virus gene expression with only the latent virus proteins (LVPs) upregulated in both tumors and LCLs. In this review, we summarize some clinical and epidemiological features of EBV- associated tumors. We also discuss how EBV latent genes may lead to oncogenesis in the different clinical malignancies","PeriodicalId":23111,"journal":{"name":"The Open Virology Journal","volume":"10 1","pages":"7 - 28"},"PeriodicalIF":0.0,"publicationDate":"2015-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87688966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 40

Exploration of New Sites in Adenovirus Hexon for Foreign Peptides Insertion. 腺病毒六邻体外源肽插入新位点的探索。

The Open Virology Journal Pub Date : 2015-05-29 eCollection Date: 2015-01-01 DOI: 10.2174/1874357901509010001

Satyender Hansra, Sujit Pujhari, Alexander N Zakhartchouk

{"title":"Exploration of New Sites in Adenovirus Hexon for Foreign Peptides Insertion.","authors":"Satyender Hansra, Sujit Pujhari, Alexander N Zakhartchouk","doi":"10.2174/1874357901509010001","DOIUrl":"https://doi.org/10.2174/1874357901509010001","url":null,"abstract":"Adenoviral vectors are now being explored as vaccine carriers to prevent infectious diseases in humans and animals. There are two strategies aimed at the expression of a vaccine antigen by adenoviral vectors. The first includes an insertion of the foreign gene expression cassette into the E1 region. The second strategy is antigen incorporation into the viral capsid proteins. To extend this methodology, we have searched for new sites at the human adenovirus serotype 5 major capsid protein hexon for a vaccine antigen insertion. To this end, we utilized sites in the hexon hypervariable region (HVR) 7, 8 and 9 to display a 15-mer peptide containing the main neutralizing epitope of porcine reproductive and respiratory syndrome virus. However, we could not rescue the viruses with the insertions of the peptide into HVR 8 and 9, consistent with the viruses being unable to tolerate insertions at these sites. In contrast, the virus with the insertion of the peptide in HVR 7 was viable - growing well in cell culture and the inserted peptide was exposed on the virion surface. ","PeriodicalId":23111,"journal":{"name":"The Open Virology Journal","volume":"9 ","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2015-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/53/a3/TOVJ-9-1.PMC4460227.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33383777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Disease caused by rotavirus infection. 轮状病毒感染引起的疾病。

The Open Virology Journal Pub Date : 2014-12-11 eCollection Date: 2014-01-01 DOI: 10.2174/1874357901408010014

Che-Liang Lin, Shou-Chien Chen, Shyun-Yeu Liu, Kow-Tong Chen

引用次数: 20

Influenza A H1N1pdm 2009 Virus in Paraguay: Nucleotide Point Mutations in Hemagglutinin and Neuraminidase Genes are not Associated with Drug Resistance. 巴拉圭甲型H1N1pdm 2009病毒:血凝素和神经氨酸酶基因的核苷酸点突变与耐药性无关

The Open Virology Journal Pub Date : 2014-09-29 eCollection Date: 2014-01-01 DOI: 10.2174/1874357901408010009

Emilio E Espínola, Alberto A Amarilla, Magaly Martínez, Víctor H Aquino, Graciela Russomando

{"title":"Influenza A H1N1pdm 2009 Virus in Paraguay: Nucleotide Point Mutations in Hemagglutinin and Neuraminidase Genes are not Associated with Drug Resistance.","authors":"Emilio E Espínola, Alberto A Amarilla, Magaly Martínez, Víctor H Aquino, Graciela Russomando","doi":"10.2174/1874357901408010009","DOIUrl":"https://doi.org/10.2174/1874357901408010009","url":null,"abstract":"Influenza virus is associated with upper respiratory tract infections. The fourth influenza pandemic was declared in 2009. The aim of this study was to determine the genetic variability of the 2009 H1N1 pandemic virus circulating in Paraguay. Nasal swabs were collected from 181 patients with flu symptoms managed at the Hospital of the Medical School in Asunción, Paraguay, between August and October 2009. Virus detection was carried out by real-time reverse transcription-polymerase chain reaction, followed by sequencing of the hemagglutinin and neuraminidase genes, and phylogenetic analysis. H1N1pdm09 was detected in 14.9% (27/181) of the suspected cases. Analysis of 13 samples showed that these viruses the clustered in a single genetic group. Neither the mutation related to exacerbation of disease (D239G in hemagglutinin) nor that related to antiviral resistance (H275Y in neuraminidase), both detected in neighboring countries, were found. This genetic analysis of H1N1pdm09 will help to understand the spread of the disease. ","PeriodicalId":23111,"journal":{"name":"The Open Virology Journal","volume":"8 ","pages":"9-13"},"PeriodicalIF":0.0,"publicationDate":"2014-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/44/8f/TOVJ-8-9.PMC4200701.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32759306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Both Cyclophilin Inhibitors and Direct-Acting Antivirals Prevent PKR Activation in HCV-Infected Cells. 亲环蛋白抑制剂和直接作用抗病毒药物均可阻止hcv感染细胞中PKR的激活。

The Open Virology Journal Pub Date : 2014-03-07 eCollection Date: 2014-01-01 DOI: 10.2174/1874357901408010001

Michael Bobardt, Udayan Chatterji, Precious Lim, Katarzyna Gawlik, Philippe Gallay

{"title":"Both Cyclophilin Inhibitors and Direct-Acting Antivirals Prevent PKR Activation in HCV-Infected Cells.","authors":"Michael Bobardt, Udayan Chatterji, Precious Lim, Katarzyna Gawlik, Philippe Gallay","doi":"10.2174/1874357901408010001","DOIUrl":"https://doi.org/10.2174/1874357901408010001","url":null,"abstract":"We and others demonstrated that the contact between NS5A and the host factor CypA is critical for HCV replication. CypI, by disrupting NS5A-CypA complexes, block HCV replication both in vitro and in patients. Since NS5A also binds to PKR, a central component of the IFN response, we investigated the possibility of a relationship between CypA, NS5A and PKR in the IFN response to HCV. HCV-infected cells treated with CypI, DAAs or IFN were analyzed for the expression and activation of various components of the innate response. We found that CypI (cyclosporine A, alisporivir, NIM811 and sanglifehrins), drastically prevented the activation/phosphorylation, but not the expression of IFN-induced PKR in HCV-infected cells. CypI had no effect on the expression or phosphorylation of other components of the innate response such as eiF2, NF-kB, IRF3, IRF9, STAT1 and STAT2, suggesting a specific effect on PKR. No significant activation of IFN-induced PKR was observed in the absence of HCV. Importantly, we found that several classes of DAAs such as NS3/4A protease, NS5B polymerase and NS5A inhibitors also prevented PKR activation. Furthermore, we found that PKR activation by the dsRNA mimic poly I:C cannot be prevented by CypI or DAAs. Our findings suggest that CypI do not have a unique effect on PKR activation, but rather the suppression of HCV replication by any anti-HCV inhibitor, abrogates PKR activation induced by IFN. Moreover, they suggest that the accumulation of dsRNA intermediates allows HCV to exploit the activation of PKR to counteract the IFN response. ","PeriodicalId":23111,"journal":{"name":"The Open Virology Journal","volume":"8 ","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2014-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9e/ba/TOVJ-8-1.PMC4009744.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32316736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Recombinant Salmonella Bacteria Vectoring HIV/AIDS Vaccines. 重组沙门氏菌载体HIV/AIDS疫苗。

The Open Virology Journal Pub Date : 2013-12-30 eCollection Date: 2013-01-01 DOI: 10.2174/1874357901307010121

Nyasha Chin'ombe, Vurayai Ruhanya

引用次数: 9

Fulminant Hepatitis Due to Father-to-Newborn Transmission of Herpes Simplex Virus type 1. 单纯疱疹病毒1型由父亲传染给新生儿的暴发性肝炎。

The Open Virology Journal Pub Date : 2013-10-31 eCollection Date: 2013-01-01 DOI: 10.2174/1874357901307010096

A Bal, C Zandotti, A Nougairede, L Ninove, B Roquelaure, R N Charrel

引用次数: 6

Viral and Cellular Components of AAV2 Replication Compartments. AAV2复制区室的病毒和细胞成分。

The Open Virology Journal Pub Date : 2013-10-31 eCollection Date: 2013-01-01 DOI: 10.2174/1874357901307010098

Rebecca Vogel, Michael Seyffert, Bruna de Andrade Pereira, Cornel Fraefel

引用次数: 8