The Journal of laboratory and clinical medicine最新文献

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Distribution of iodine 125-labeled alpha1-microglobulin in rats after intravenous injection. 碘125标记α - 1微球蛋白在大鼠静脉注射后的分布。
The Journal of laboratory and clinical medicine Pub Date : 2001-03-01 DOI: 10.1067/MLC.2001.112957
Jörgen Larsson, K. Wingårdh, T. Berggård, Julia R. Davies, Lennart Lögdberg, Sven-Erik Strand, Bo Åkerström
{"title":"Distribution of iodine 125-labeled alpha1-microglobulin in rats after intravenous injection.","authors":"Jörgen Larsson, K. Wingårdh, T. Berggård, Julia R. Davies, Lennart Lögdberg, Sven-Erik Strand, Bo Åkerström","doi":"10.1067/MLC.2001.112957","DOIUrl":"https://doi.org/10.1067/MLC.2001.112957","url":null,"abstract":"The 28-kd plasma protein alpha(1)-microglobulin is found in the blood of mammals and fish in a free, monomeric form and as high-molecular-weight complexes with molecular masses above 200 kd. In this study, iodine 125-labeled free and high-molecular weight rat alpha(1)-microglobulin (a mixture of alpha(1)-microglobulin/alpha(1)-inhibitor-3 and alpha(1)-microglobulin/fibronectin complexes) were injected intravenously into rats. The distribution of the proteins was measured by using scintillation camera imaging. Both forms of (125)I-labeled alpha(1)-microglobulin were rapidly cleared from the blood, with a half-life of 2 and 16 minutes for the initial and late phase, respectively, for free alpha(1)-microglobulin; and a half-life of 3 and 130 minutes for the initial and late phase, respectively, for the complexes. After 45 minutes, 6%, 16%, 27%, 13%, and 34% of the free (125)I-labeled alpha(1)-microglobulin and 18%, 21%, 6%, 10%, and 42% of the (125)I-labeled alpha(1)-microglobulin complexes were found in the blood, gastrointestinal tract, kidneys, liver, and the remainder of the body, respectively. The local distribution of injected (125)I-labeled alpha(1)-microglobulin in intestines and kidneys was investigated by microscopy and autoradiography. In the intestine, both forms were distributed in the basal layers, villi, and luminal contents. The results also suggested intracellular labeling of epithelial cells. Well-defined local regions containing higher concentrations of injected protein could be seen in the intestine. In the kidneys, both forms were found mostly in the cortex. Free (125)I-labeled alpha(1)-microglobulin was found predominantly in epithelial cells of a subset of the tubules, whereas the (125)I-labeled complexes were more evenly distributed. Intracellular labeling was indicated for both alpha(1)-microglobulin forms. The results thus indicate a rapid transport of (125)I-labeled alpha(1)-microglobulin from the blood to most tissues.","PeriodicalId":23085,"journal":{"name":"The Journal of laboratory and clinical medicine","volume":"17 1","pages":"165-75"},"PeriodicalIF":0.0,"publicationDate":"2001-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89272423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 47
Adenovirus-mediated transfer of the atrial natriuretic peptide gene in rat pulmonary vascular smooth muscle cells leads to apoptosis. 腺病毒介导的心房利钠肽基因在大鼠肺血管平滑肌细胞中的转移导致细胞凋亡。
The Journal of laboratory and clinical medicine Pub Date : 2001-03-01 DOI: 10.1067/MLC.2001.112725
I. Déprez, M. Darmon, M. Hira, M. Adam, S. Sanquer, E. Teiger, V. Chetboul, M. Eloit, S. Adnot, I. Pham
{"title":"Adenovirus-mediated transfer of the atrial natriuretic peptide gene in rat pulmonary vascular smooth muscle cells leads to apoptosis.","authors":"I. Déprez, M. Darmon, M. Hira, M. Adam, S. Sanquer, E. Teiger, V. Chetboul, M. Eloit, S. Adnot, I. Pham","doi":"10.1067/MLC.2001.112725","DOIUrl":"https://doi.org/10.1067/MLC.2001.112725","url":null,"abstract":"Atrial natriuretic peptide (ANP) exhibits relaxant and growth-inhibiting effects on vascular smooth muscle cells (VSMCs). To obtain ANP gene expression in VSMCs, we built a recombinant adenovirus containing the ANP cDNA controlled by the adenovirus major late promotor (AdMLP-ANP). After pulmonary VSMC treatment with AdMLP-ANP at a multiplicity of infection ranging from 5 to 100 TCID(50)/cell, immunoreactive ANP was detectable in the cell culture medium at a level that reached 101 +/- 27 pmol/well after 2 days. The newly expressed ANP was biologically active, as evidenced by its ability to induce cyclic guanosine monophosphate accumulation in target cells and to mimic the effect of exogenous ANP (10(-8) to 10(-7) mol/L). Cell growth and survival of AdMLP-ANP-infected cells were decreased and were associated with the promotion of VSMC apoptosis. These effects, which occurred at a multiplicity of infection of 10 to 100 TCID(50)/cell, were observed neither in cells infected with the control adenoviral constructs (AdMLP-betaGAL and AdMLP-gD) nor in cells treated with exogenous ANP (10(-7) to 10(-6) mol/L). These results showing VSMC apoptosis in response to ANP gene expression may have important implications for the prevention of vascular remodeling by gene therapy.","PeriodicalId":23085,"journal":{"name":"The Journal of laboratory and clinical medicine","volume":"75 1","pages":"155-64"},"PeriodicalIF":0.0,"publicationDate":"2001-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83806982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Natural history of chronic hepatitis C: identifying a window of opportunity for intervention. 慢性丙型肝炎的自然史:确定干预的机会之窗。
The Journal of laboratory and clinical medicine Pub Date : 2001-03-01 DOI: 10.1016/S0011-5029(01)90006-9
Huy A. Nguyen, Samuel B. Ho
{"title":"Natural history of chronic hepatitis C: identifying a window of opportunity for intervention.","authors":"Huy A. Nguyen, Samuel B. Ho","doi":"10.1016/S0011-5029(01)90006-9","DOIUrl":"https://doi.org/10.1016/S0011-5029(01)90006-9","url":null,"abstract":"","PeriodicalId":23085,"journal":{"name":"The Journal of laboratory and clinical medicine","volume":"42 1","pages":"146-54"},"PeriodicalIF":0.0,"publicationDate":"2001-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85290495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Effect of erythromycin on matrix metalloproteinase-9 and cell migration. 红霉素对基质金属蛋白酶-9及细胞迁移的影响。
The Journal of laboratory and clinical medicine Pub Date : 2001-03-01 DOI: 10.1067/MLC.2001.112726
N. Hashimoto, T. Kawabe, T. Hara, K. Imaizumi, H. Wakayama, H. Saito, K. Shimokata, Y. Hasegawa
{"title":"Effect of erythromycin on matrix metalloproteinase-9 and cell migration.","authors":"N. Hashimoto, T. Kawabe, T. Hara, K. Imaizumi, H. Wakayama, H. Saito, K. Shimokata, Y. Hasegawa","doi":"10.1067/MLC.2001.112726","DOIUrl":"https://doi.org/10.1067/MLC.2001.112726","url":null,"abstract":"Erythromycin (EM) has an anti-inflammatory effect that may account for its clinical benefit in the treatment of chronic inflammatory diseases such as diffuse panbronchiolitis. To investigate the mechanism of this anti-inflammatory effect, we studied the relationship between the concentration of EM and matrix metalloproteinase-9 (MMP-9) activity, which is important in cell migration. We showed that EM suppressed the gelatinolytic activity of U937 cell-derived MMP-9 by using gelatin zymography, showed that expressions of MMP-9 protein and MMP-9 mRNA were down-regulated by EM in a dose-dependent manner, and showed that U937 migration was also suppressed by EM. We also demonstrated that EM treatment suppressed the gelatinolytic activity of MMP-9 in spleen macrophages. These findings suggest that the suppressive effect of EM on MMP-9 activity is one of the anti-inflammatory mechanisms that inhibit the migration of inflammatory cells into the inflammatory site.","PeriodicalId":23085,"journal":{"name":"The Journal of laboratory and clinical medicine","volume":"10 1","pages":"176-83"},"PeriodicalIF":0.0,"publicationDate":"2001-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74788844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 38
Vascular endothelial growth factor in peritoneal dialysis: a longitudinal follow-up. 血管内皮生长因子在腹膜透析:纵向随访。
The Journal of laboratory and clinical medicine Pub Date : 2001-02-01 DOI: 10.1067/MLC.2001.112235
M. M. Zweers, D. Struijk, W. Smit, R. Krediet
{"title":"Vascular endothelial growth factor in peritoneal dialysis: a longitudinal follow-up.","authors":"M. M. Zweers, D. Struijk, W. Smit, R. Krediet","doi":"10.1067/MLC.2001.112235","DOIUrl":"https://doi.org/10.1067/MLC.2001.112235","url":null,"abstract":"In a previous study, vascular endothelial growth factor (VEGF) was found to be locally produced in the peritoneal tissue of patients undergoing peritoneal dialysis (PD) who were being treated with glucose-containing PD solutions. Locally produced VEGF (LVEGF) was positively related to the mass transfer area coefficient (MTAC) of creatinine and to glucose absorption, both of which are representative of the peritoneal vascular surface area. It was therefore hypothesized that VEGF is involved in the peritoneal neoangiogenesis found in long-term PD. The aim of the present study was to investigate the time course of peritoneal VEGF levels in PD patients treated with glucose-based PD solutions during longitudinal follow-up. We also studied the effect of the switch to glucose-free PD treatment on VEGF production. Forty standard peritoneal permeability analyses (SPAs) with 3.86% glucose-containing dialysis solution were investigated. The SPAs were performed in 10 PD patients with a median number of three SPAs per patient during a follow-up of 23 months. Duration of PD treatment at the last SPA was 74 months. All patients were initially treated with glucose-containing dialysis solutions. Four patients switched after 114 months of glucose-based PD to glucose-free PD and were followed for 7 months. A PD regimen of icodextrin, glycerol, and amino acid-based dialysis solutions was applied in these patients. Four SPAs were performed per patient in this period. To predict the VEGF dialysate-to-serum ratio (D/S), when diffusion would be the only explanation for the VEGF dialysate concentration, we calculated the power relationship between D/S ratios of serum proteins that are only transported across the peritoneum and the molecular weights of those proteins. The measured VEGF D/S ratio was higher than expected (P <.001) in each observation, pointing to local production of VEGF. LVEGF increased with duration of glucose PD, 11.7 ng/L to 23.45 ng/L (P <.03). LVEGF decreased in all 4 patients undergoing glucose-free PD, from 57.35 ng/L to 23.10 ng/L. A correlation (r = 0.83, P <.001) was found be-tween the differences in MTAC creatinine between the first and last SPA during glucose-based PD and the difference in LVEGF between these observations. A similar correlation was present between the difference in glucose absorption and the difference in LVEGF (r = 0.85, P <.001). This supports a pathogenetic role of high glucose dialysate concentrations in the development of changes in the peritoneum that are found in long-term PD. Treatment with non-glucose-based PD solutions may inhibit further development of these alterations.","PeriodicalId":23085,"journal":{"name":"The Journal of laboratory and clinical medicine","volume":"16 1","pages":"125-32"},"PeriodicalIF":0.0,"publicationDate":"2001-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77738383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 107
cDNA cloning and immunologic characterization of Pen o 18, the vacuolar serine protease major allergen of Penicillium oxalicum. 草青霉液泡丝氨酸蛋白酶主要变应原p18cdna克隆及免疫特性研究。
The Journal of laboratory and clinical medicine Pub Date : 2001-02-01 DOI: 10.1067/MLC.2001.112096
H. Shen, C. Wang, W. L. Lin, H. Lai, M. Tam, H. Chou, S. R. Wang, S. H. Han
{"title":"cDNA cloning and immunologic characterization of Pen o 18, the vacuolar serine protease major allergen of Penicillium oxalicum.","authors":"H. Shen, C. Wang, W. L. Lin, H. Lai, M. Tam, H. Chou, S. R. Wang, S. H. Han","doi":"10.1067/MLC.2001.112096","DOIUrl":"https://doi.org/10.1067/MLC.2001.112096","url":null,"abstract":"Penicillium species are prevalent indoor airborne fungi that have been identified as causative agents of human extrinsic bronchial asthma. In the preparation of standardized diagnostic reagents, it is imperative to define the allergens of these ubiquitous fungi. Results from our previous study on P. oxalicum suggest that the 34-kd major immunoglobulin E-reacting component of this prevalent Penicillium species is probably a vacuolar serine protease. The purpose of the present study was to define this major P. oxalicum allergen (Pen o 18) through cDNA cloning and immunologic characterization. The cDNA of Pen o 18 was isolated through a combination of reverse transcriptase-polymerase chain reaction and 5'- and 3'-rapid amplification cDNA ends reactions. The primers used in these reactions were constructed according to the internal amino acid sequences of Pen o 18 and the conserved amino acid sequences of fungal serine proteases. Our results showed that a 1897-bp cDNA with an open reading frame of 503 residues was isolated for the proenzyme of Pen o 18. The encoded protein has a 16-residue signal peptide and a 119-residue prosequence. On maturation, the protein has an N-terminal glutamate that is the 136th residue encoded by the cDNA. Apparently the precursor also undergoes C-terminal processing with the cleavage of about 47 amino acids. The cDNA for Pen c 18 (the vacuolar serine protease allergen from P. citrinum ) was also isolated for comparison. Contrary to a previous report, the C-terminal region of Pen c 18 is similar to that of Pen o 18. Recombinant proteins (rPen o 18 and rPen c 18) with the putative mature N-termini and a his-tag were obtained by expressing the corresponding cDNAs in Escherichia coli. Serum samples from 7 asthmatic patients with immunoglobulin E reactivity to the 34-kd component of P. oxalicum also react to his-tagged recombinant Pen o 18. The presence of immunoglobulin E cross-reactivity between rPen o 18 and rPen c 18 was detected by immunoblot inhibition. Two monoclonal antibodies (PCM39 and FUM20) against fungal serine proteases react with rPen o 18, rPen c 18, and the 35/34-kd components in the corresponding crude fungal extracts. These components also react with immunoglobulin E antibodies in serum samples from asthmatic patients. In conclusion, results obtained confirm that the 34-kd major allergen of P. oxalicum is a vacuolar serine protease. The cDNAs of Pen o 18 and Pen c 18 encode precursor molecules that appear to undergo both N-terminal and C-terminal processing. Constructs beginning with mature N-terminal can be expressed in E. coli to produce recombinant polypeptides that are reactive to monoclonal antibodies or immunoglobulin E antibodies in serum samples from asthmatic patients. Results obtained may provide useful information and materials for preparation of standardized diagnostic reagents in clinical mold allergy.","PeriodicalId":23085,"journal":{"name":"The Journal of laboratory and clinical medicine","volume":"9 1","pages":"115-24"},"PeriodicalIF":0.0,"publicationDate":"2001-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87494348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 40
ApaI polymorphisms of the vitamin D receptor predict bone density of the lumbar spine and not racial difference in bone density in young men. 维生素D受体ApaI多态性预测腰椎骨密度,而不是年轻男性骨密度的种族差异。
The Journal of laboratory and clinical medicine Pub Date : 2001-02-01 DOI: 10.1067/MLC.2001.112095
N. Bell, N. Morrison, Tuan V. Nguyen, J. Eisman, B. Hollis
{"title":"ApaI polymorphisms of the vitamin D receptor predict bone density of the lumbar spine and not racial difference in bone density in young men.","authors":"N. Bell, N. Morrison, Tuan V. Nguyen, J. Eisman, B. Hollis","doi":"10.1067/MLC.2001.112095","DOIUrl":"https://doi.org/10.1067/MLC.2001.112095","url":null,"abstract":"A number of previous investigations showed significant associations between polymorphisms of the vitamin D receptor (VDR) gene and bone mineral density (BMD). BMD is influenced by hormones and the rate of skeletal remodeling. A study was performed to investigate the possible relationship between Apa I, Bsm I, Taq I, and Fok I polymorphisms of the VDR gene and serum 1,25-dihydroxyvitamin D (1,25[OH]2D), osteocalcin, and propeptide of type I collagen (PICP)-markers of bone turnover, total body calcium, and BMD of the total body, radius, lumbar spine, trochanter, and femoral neck-in 39 young adult black men of 20 to 40 years of age and 44 age-, height-, and weight-matched white men. The distribution of each of the four alleles of the VDR genotypes was similar in the two racial groups. The Apa I VDR genotype was associated with serum PICP (P =.0494) but not with serum 1,25(OH)2D or serum osteocalcin. A significant association between the Apa I VDR genotype and BMD of the lumbar spine (P =.0291) was also observed. However, the Bsm I, Taq I, and Fok I genotypes were not significantly associated with BMD or serum osteocalcin, PICP, or 1,25(OH)2D. Multivariate stepwise analysis indicated that (1) the Apa I VDR genotype was associated with BMD of the lumbar spine in the two groups together; with total body calcium and BMD of the total body, radius, trochanter, and femoral neck in the black men; and with BMD of the radius in the white men; analysis also indicated that (2) race was significantly associated with total body calcium and BMD of the total body, lumbar spine, and femoral neck. In summary, the Apa I VDR genotype is associated with serum PICP and BMD at a number of sites but does not contribute to or account for racial differences in BMD in young adult men.","PeriodicalId":23085,"journal":{"name":"The Journal of laboratory and clinical medicine","volume":"222 1","pages":"133-40"},"PeriodicalIF":0.0,"publicationDate":"2001-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74669799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 33
An analysis of renal nitric oxide contribution to hyperfiltration in diabetic rats. 肾脏一氧化氮对糖尿病大鼠高滤过的作用分析。
The Journal of laboratory and clinical medicine Pub Date : 2001-02-01 DOI: 10.1067/MLC.2001.112691
D. Schwartz, I. Schwartz, R. Blantz
{"title":"An analysis of renal nitric oxide contribution to hyperfiltration in diabetic rats.","authors":"D. Schwartz, I. Schwartz, R. Blantz","doi":"10.1067/MLC.2001.112691","DOIUrl":"https://doi.org/10.1067/MLC.2001.112691","url":null,"abstract":"We have investigated whether nitric oxide (NO) generation is increased in diabetes and whether specific NO synthase (NOS) isoforms are up-regulated in 4-week diabetic male Wistar rats. Glomerular filtration rate (GFR), kidney weight, and urinary nitrate (NOx) generation were measured in the following groups (n = 6): normal control animals, diabetic animals, diabetic animals given L -NIL (a selective iNOS inhibitor)(D + L -NIL), diabetic animals given L -NAME (a nonselective NOS inhibitor)(D + L -NAME), and control animals given L -NAME (C + L -NAME). Diabetes increased GFR (0.78 +/- 0.05 mL/min/100 g body wt vs 1.49 +/- 0.07 mL/min/100 g body wt, P <.01). L -NIL did not affect hyperfiltration, while L -NAME decreased GFR to values that were lower than those in normal control animals, a response identical to that in non-diabetic control rats. L -NIL did not affect urinary NOx values, but L -NAME completely abolished the increase in urinary nitrates. Kidney weight was not affected by L -NIL, but L -NAME significantly attenuated kidney growth. Inducible NOS (iNOS) and endothelial NOS (eNOS) mRNA levels measured by reverse transcription-polymerase chain reaction in diabetic rats were not changed as compared with levels in controls. Cyclic guanosine monophosphate responses to carbachol (an index of eNOS activity) in glomeruli from diabetic rats were significantly reduced as compared with those in controls, and guanylate cyclase responses to sodium nitroprusside were significantly decreased. Therefore, renal NO generation, at least via eNOS and iNOS, is not the primary cause of glomerular hyperfiltration in diabetes.","PeriodicalId":23085,"journal":{"name":"The Journal of laboratory and clinical medicine","volume":"30 1","pages":"107-14"},"PeriodicalIF":0.0,"publicationDate":"2001-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88046501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 28
Manipulating the vascular biology of coronary atherosclerosis in diabetes: new opportunities. 控制糖尿病冠状动脉粥样硬化的血管生物学:新的机遇。
The Journal of laboratory and clinical medicine Pub Date : 2001-02-01 DOI: 10.1067/MLC.2001.110970
J. Orford, S. Kinlay, J. Fernandes, D. Behrendt, P. Ganz, A. Selwyn
{"title":"Manipulating the vascular biology of coronary atherosclerosis in diabetes: new opportunities.","authors":"J. Orford, S. Kinlay, J. Fernandes, D. Behrendt, P. Ganz, A. Selwyn","doi":"10.1067/MLC.2001.110970","DOIUrl":"https://doi.org/10.1067/MLC.2001.110970","url":null,"abstract":"ore than 7 million people in the United States of America have diabetes mellitus, and type 2 diabetes and the insulin resistance syndrome are increasingly prevalent. This is thought to be the result of the aging of the population, the increasing prevalence of obesity, the growing contribution of highrisk minority groups, and a lower glycemic threshold for the diagnosis of diabetes. The insulin resistance syndrome (syndrome X) describes the clustering of a number of metabolic abnormalities that are risk factors for both diabetes mellitus and atherosclerotic cardiovascular disease.1 These risk factors include hyperinsulinemia, impaired glucose tolerance, hypertension, and a characteristic dyslipidemia (Table I).2 An alternative name, the cardiovascular dysmetabolic syndrome, has been proposed in an effort to emphasize the cardiovascular consequences of this syndrome (Table II).3 Accelerated atherosclerosis is a common and very important feature of both diabetes and insulin resistance, and atherosclerotic cardiovascular disease is responsible for at least three quarters of all diabetesrelated deaths.4,5 Microvascular complications of diabetes are improved by maintenance of tight long-term glycemic control, whereas it has proved difficult to show that atherosclerosis of the large conduit arteries and its complications are prevented by glycemic control.6,7 Aggressive treatment of cardiovascular risk factors associated with the insulin resistance syndrome and diabetes mellitus, including hypertension and dyslipidemia, is effective in reducing the relative risk of cardiovascular events in diabetic patients.8,9 This review focuses on the cell/vessel wall dysfunctions in these syndromes that cause atherosclerosis and the new therapeutic opportunities that follow.","PeriodicalId":23085,"journal":{"name":"The Journal of laboratory and clinical medicine","volume":"67 1","pages":"82-92"},"PeriodicalIF":0.0,"publicationDate":"2001-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82191418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Protective effect of anisodamine against Shiga toxin-1: inhibition of cytokine production and increase in the survival of mice. 山莨菪碱对志贺毒素1的保护作用:抑制细胞因子的产生,提高小鼠的存活率。
The Journal of laboratory and clinical medicine Pub Date : 2001-02-01 DOI: 10.1067/MLC.2001.112507
H. Zhang, Z. Ou, F. Gondaira, M. Ohmura, S. Kojio, T. Yamamoto
{"title":"Protective effect of anisodamine against Shiga toxin-1: inhibition of cytokine production and increase in the survival of mice.","authors":"H. Zhang, Z. Ou, F. Gondaira, M. Ohmura, S. Kojio, T. Yamamoto","doi":"10.1067/MLC.2001.112507","DOIUrl":"https://doi.org/10.1067/MLC.2001.112507","url":null,"abstract":"The purpose of this study was to investigate whether anisodamine could inhibit Shiga toxin-1 (Stx1)-induced cytokine production and increase the survival of Stx1-treated mice. Human monocytic cells were stimulated by Stx1 (1 to 100 ng/mL) with or without anisodamine addition (1 to 400 microg/mL). For in vivo evaluations, C57BL/6 mice were given a single intraperitoneal injection of anisodamine (1 mg) or saline solution after intraperitoneal injection of Stx1 (2.75 microg/kg). The results showed that anisodamine significantly suppressed Stx1-induced tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and IL-8 production. Reverse transcriptase-polymerase chain reaction (RT-PCR) showed that anisodamine suppressed Stx1-mediated TNF-alpha mRNA expression. Further study showed that this TNF-alpha inhibitory effect was via a prostaglandin E2-dependent mechanism. Anisodamine treatment prolonged the survival time of mice and decreased the lethality of Stx1 (94.5% to 44%). Because cytokines, in particular TNF-alpha, contribute to the pathologic process in Stx-producing Escherichia coli (STEC) infection, this study suggested that anisodamine could be a potential drug for treatment of STEC infection.","PeriodicalId":23085,"journal":{"name":"The Journal of laboratory and clinical medicine","volume":"95 1","pages":"93-100"},"PeriodicalIF":0.0,"publicationDate":"2001-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73227102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
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