Effect of erythromycin on matrix metalloproteinase-9 and cell migration.

N. Hashimoto, T. Kawabe, T. Hara, K. Imaizumi, H. Wakayama, H. Saito, K. Shimokata, Y. Hasegawa
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引用次数: 38

Abstract

Erythromycin (EM) has an anti-inflammatory effect that may account for its clinical benefit in the treatment of chronic inflammatory diseases such as diffuse panbronchiolitis. To investigate the mechanism of this anti-inflammatory effect, we studied the relationship between the concentration of EM and matrix metalloproteinase-9 (MMP-9) activity, which is important in cell migration. We showed that EM suppressed the gelatinolytic activity of U937 cell-derived MMP-9 by using gelatin zymography, showed that expressions of MMP-9 protein and MMP-9 mRNA were down-regulated by EM in a dose-dependent manner, and showed that U937 migration was also suppressed by EM. We also demonstrated that EM treatment suppressed the gelatinolytic activity of MMP-9 in spleen macrophages. These findings suggest that the suppressive effect of EM on MMP-9 activity is one of the anti-inflammatory mechanisms that inhibit the migration of inflammatory cells into the inflammatory site.
红霉素对基质金属蛋白酶-9及细胞迁移的影响。
红霉素(EM)具有抗炎作用,这可能是其治疗慢性炎症性疾病(如弥漫性泛细支气管炎)的临床益处的原因。为了探讨这种抗炎作用的机制,我们研究了EM浓度与基质金属蛋白酶-9 (MMP-9)活性的关系,MMP-9在细胞迁移中起重要作用。我们通过明胶酶谱法发现EM抑制了U937细胞源性MMP-9的溶胶活性,EM以剂量依赖的方式下调了MMP-9蛋白和MMP-9 mRNA的表达,EM也抑制了U937的迁移。我们还发现EM处理抑制了脾脏巨噬细胞中MMP-9的溶胶活性。这些发现提示,EM对MMP-9活性的抑制作用是抑制炎症细胞向炎症部位迁移的抗炎机制之一。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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