The Ukrainian Biochemical Journal最新文献_第6页

Genome secrets of extinct hominids, or can paleogenomics answer the question: humankind, who we are? Nobel Prize in Physiology or Medicine 2022 已经灭绝的原始人的基因组秘密，或者古基因组学能回答这个问题:人类，我们是谁?2022年诺贝尔生理学或医学奖

The Ukrainian Biochemical Journal Pub Date : 2023-04-26 DOI: 10.15407/ubj95.01.103

S. Komisarenko, S. Romaniuk

引用次数: 0

CRISPR as a novel technique for COVID-19 diagnosis: a review CRISPR作为新型COVID-19诊断技术综述

The Ukrainian Biochemical Journal Pub Date : 2023-04-26 DOI: 10.15407/ubj95.01.005

A. Saleem, A. Annooz, M. A. Hadi, A. H. H. Kabla

引用次数: 0

Fatty acids composition of actinobacteria isolated from mussels Mytilus galloprovincialis of the Black Sea Odesa Bay 黑海敖德萨湾贻贝(Mytilus galloprovincialis)放线菌的脂肪酸组成

The Ukrainian Biochemical Journal Pub Date : 2023-04-26 DOI: 10.15407/ubj95.01.085

N. Korotaeva, K. S. Potapenko, Z. Y. Sergieieva, N. Vasylieva, V. Ivanytsia

{"title":"Fatty acids composition of actinobacteria isolated from mussels Mytilus galloprovincialis of the Black Sea Odesa Bay","authors":"N. Korotaeva, K. S. Potapenko, Z. Y. Sergieieva, N. Vasylieva, V. Ivanytsia","doi":"10.15407/ubj95.01.085","DOIUrl":"https://doi.org/10.15407/ubj95.01.085","url":null,"abstract":"Analysis of the fatty acid composition of total cellular lipids is important chemotaxonomic characteristic that is used to identify microorganisms, in particular actinobacteria, with the help of fatty acid spectra libraries. Actinobacteria are well known as producers of secondary metabolites, which are of pharmacological and commercial interest. A significant number of actinomycetes is associated with various marine benthic communities. The aim of this study was determination of the fatty acid composition and preliminary identification of actinobacteria isolated from mussels Mytilus galloprovincialis of the Black Sea Odesa Bay. Actinobacteria of 12 isolated strains were grown in a liquid medium at 28° C for 72 h. Methyl esters of fatty acids were determined on a gas chromatograph Agilent. The MIDI Sherlock microorganisms identification system was used to identify the studied strains. It was found that the fatty acids methyl esters profiles of studied actinobacteria strains were characterized by the predominance of 12-17 saturated branched-chain fatty acids with high content of 12-methyltridecanoic, 12-methyltetradecanoic, 14-methylpentadecanoic and 14-methylhexadecanoic fatty acids. All 12 actinobacteria strains isolated from mussels (Mytilus galloprovincialis) of Odesa Bay were identified as members of the Streptomyces genus. Keywords: Black Sea, fatty acid compositions, marine actinobacteria, Mytilus galloprovincialis, Streptomyces","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80487308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Agmatine, telomerase and trace minerals levels in patients with obstructive sleep apnea syndrome 阻塞性睡眠呼吸暂停综合征患者的胍丁氨酸、端粒酶和微量矿物质水平

The Ukrainian Biochemical Journal Pub Date : 2023-02-23 DOI: 10.15407/ubj94.06.037

D. Ülger, S. Bakır, Ö. Doğan

{"title":"Agmatine, telomerase and trace minerals levels in patients with obstructive sleep apnea syndrome","authors":"D. Ülger, S. Bakır, Ö. Doğan","doi":"10.15407/ubj94.06.037","DOIUrl":"https://doi.org/10.15407/ubj94.06.037","url":null,"abstract":"Obstructive sleep apnea syndrome (OSAS) is a disease characterized by repetitive, partially or complete upper airway obstructions resulting in hypoxia and bioelectrical wakefulness reactions along with sleep. There is a limited and different information on the biochemical pathways that may determine harmful effects in OSAS patients with different disease severity. Hence, we aimed to estimate the plasma levels of polyamine agmatine, which has many effects on the central nervous system, telomerase and trace minerals in patients with OSAS. The study included 90 volunteer patients diagnosed with OSAS and divided into three groups of 30 people each according to the apnea-hypopnea index (AHI) score: mild, moderate and severe. Nocturnal blood oxygen saturation percentage (SpO2) and body mass index (BMI) were measured. Plasma agmatine level was defined by ultra-high-pressure liquid chromatography (UHPLC), plasma trace elements (Cu, Co, Mg, Mo, Zn, Se) level by inductively coupled plasma mass spectrometer (ICP-MS) and serum telomerase level by enzyme-linked immunosorbent assay (ELISA) method. It was found that SpO2 value decreased as the disease progressed and showed a negative correlation with BMI, Co and Se plasma levels. The levels of agmatine and telomerase were shown to lower in patients with severe OSAS group compared to other groups. Keywords: agmatine, sleep apnea syndrome, SpO2, telomerase, trace minerals","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86698529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of magnetic resonance imaging: Paul Lauterbur and Peter Mansfield. The Nobel Prize in Physiology or Medicine 2003 磁共振成像的发现:保罗·劳特伯和彼得·曼斯菲尔德。2003年诺贝尔生理学或医学奖

The Ukrainian Biochemical Journal Pub Date : 2023-02-23 DOI: 10.15407/ubj94.06.067

V. M. Danilova, M. Grigorieva, S. Komisarenko

引用次数: 0

Computational investigation of honeybee venom proteins as potential Omicron SARS-CoV-2 inhibitors 蜂毒蛋白作为潜在的Omicron SARS-CoV-2抑制剂的计算研究

The Ukrainian Biochemical Journal Pub Date : 2023-02-23 DOI: 10.15407/ubj94.06.003

H. Al-Madhagi, M. Saleh

引用次数: 0

Influence of NF-κB on the development of oxidative-nitrosative stress in the liver of rats under conditions of chronic alcohol intoxication NF-κB对慢性酒精中毒大鼠肝脏氧化亚硝化应激发展的影响

The Ukrainian Biochemical Journal Pub Date : 2023-02-23 DOI: 10.15407/ubj94.06.057

A. Mykytenko, O. Akimov, G. Yeroshenko, K. Neporada

{"title":"Influence of NF-κB on the development of oxidative-nitrosative stress in the liver of rats under conditions of chronic alcohol intoxication","authors":"A. Mykytenko, O. Akimov, G. Yeroshenko, K. Neporada","doi":"10.15407/ubj94.06.057","DOIUrl":"https://doi.org/10.15407/ubj94.06.057","url":null,"abstract":"Alcohol-related liver disease is the most common cause of liver disease worldwide. The purpose of this work is the establishment of the influence of the transcription factor κB on the development of oxidative-nitrosative stress in the liver of rats under conditions of chronic alcohol intoxication. The experiments were performed on 24 male Wistar rats weighing 180-220 g. The animals were divided into 4 groups of 6 animals: control; animals, which were administered NF-κB inhibitor, namely ammonium pyrrolidinedithiocarbamate (PDTC) at a dose of 76 mg/kg 3 times a week; animals, on which we simulated alcoholic hepatitis and group of combination of alcoholic hepatitis and NF-κB inhibitor. We determined in rat liver homogenate the following biochemical parameters: the activity of NO synthase isoforms, superoxide dismutase and catalase activity, the concentration of malonic dialdehyde, the concentration of peroxynitrite, nitrites and nitrosothiols, concentration of sulfide anion and superoxide anion radical production. Chronic alcohol intoxication led to increased production of reactive oxygen and nitrogen species on the background of decreased antioxidant activity, thus intensifying lipid peroxidation in the liver. Blockade of the transcription factor κB during chronic alcohol intoxication despite an increase in antioxidant activity and decrease of reactive oxygen and nitrogen species production did not ameliorate oxidative damage to the liver. Blockade of activation of nuclear transcription factor κB in rat liver by PDTC reduced the risk of oxidative damage to hepatocytes, but did not reduce the risk of developing nitrosative damage to hepatocytes. Keywords: chronic alcohol intoxication, NF-κB, oxidative-nitrosative stress, PDTC, rat liver","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83606648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Prooxidant-antioxidant profile in tissues of rats under the action of thiosulfonate esters 硫代磺酸酯作用下大鼠组织中的促氧化-抗氧化谱

The Ukrainian Biochemical Journal Pub Date : 2023-02-23 DOI: 10.15407/ubj94.06.018

N. Liubas, R. Iskra, B. Kotyk, N. Monka, V. Lubenets

{"title":"Prooxidant-antioxidant profile in tissues of rats under the action of thiosulfonate esters","authors":"N. Liubas, R. Iskra, B. Kotyk, N. Monka, V. Lubenets","doi":"10.15407/ubj94.06.018","DOIUrl":"https://doi.org/10.15407/ubj94.06.018","url":null,"abstract":"Thiosulfonates are characterized by a wide spectrum of biological activity and have effective antimicrobial, antithrombotic, antitumor and antiparasitic effects. However, the use of synthetic sulfur-containing compounds for therapeutic and preventive purposes requires the study of their effect on the protective mechanisms of maintaining homeostasis, the antioxidant status of the body in particular. The aim of the study was to estimate lipid peroxidation process and the state of the antioxidant system in the kidneys, spleen, brain, and muscles of rats under the influence of newly synthesized sulfur-containing compounds of S-alkyl esters of thiosulfonic acids S-ethyl-4-aminobenzenethiosulfonate (ETS), S-allyl-4-aminobenzenethiosulfonate (ATS) and S-allyl-4-acetylaminobenzenethiosulfonate (AATS). Male Wistar rats kept on a standard diet were divided into four groups of 5 animals each: I (control), II, III, IV (experimental). The animals of the control group received additional 0.5 ml of oil and the animals of the experimental groups 0.5 ml of oil solution of thiosulfonate esters once a day. The animals of II, III and IV groups were given ETS, ATS, AATS at the rate of 100 mg per kg of body weight. The lipid hydroperoxides (LPH), TBA-active products, GSH content and activity of SOD, catalase, glutathione peroxidase, glutathione reductase in tissue homogenates were determined. The multidirectional changes of the studied indicators after consumption of S-alkyl esters of thiosulfonic acids for 21 days have been identified and characterized indicating on different degree of inhibition or activation of LPO processes depending on the type of tissue and the structure of thiosulfonate ester. Keywords: antioxidant system, rat tissues, S-allyl-4-acetylaminobenzenethiosulfonate, S-allyl-4-aminobenzenethiosulfonate, S-ethyl-4-aminobenzenethiosulfonate","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"67 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73390619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum visfatin, resistin levels and inflammation markers in psoriasis patients 银屑病患者血清内脂素、抵抗素水平及炎症标志物

The Ukrainian Biochemical Journal Pub Date : 2023-02-23 DOI: 10.15407/ubj94.06.048

A. Majid, M. Fouad

{"title":"Serum visfatin, resistin levels and inflammation markers in psoriasis patients","authors":"A. Majid, M. Fouad","doi":"10.15407/ubj94.06.048","DOIUrl":"https://doi.org/10.15407/ubj94.06.048","url":null,"abstract":"Psoriasis is a common chronic inflammatory skin condition that varies in severity. Psoriasis is associated with complex disorders, which incorporate metabolic syndrome, obesity and impaired glucose tolerance. Adipose tissue secretes several hormones and cytokines, in particular visfatin and resistin that could be involved in the development of psoriasis by acting as pro-inflammatory or immunoregulatory factors. The aim of this work was to evaluate the serum level of visfatin and resistin as well as of high-sensitivity C-reactive protein (hs-CRP) and erythrocyte sedimentation rate (ESR) in psoriatic patients. The study included 43 healthy individuals and 45 patients divided into three groups with mild, moderate and severe clinical degrees of disease assessed by the Psoriasis Area Severity Index (PASI). The results showed a significant increase in the concentration of serum visfatin, resistin, ESR and hs-CRP in patient groups in comparison with a control group. The highest increase in indicators was observed in the group of patients with severe disease compared with the mild and moderate patients groups. The significance of studied indicators as biomarkers of psoriasis disease severity is analyzed. Keywords: erythrocyte sedimentation rate, high sensitivity C-reactive protein, psoriasis, resistin, visfatin","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82393153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioenergetic characteristics of the murine Nemeth-Kellner lymphoma cells exposed to thiazole derivative in complex with polymeric nanoparticles 噻唑衍生物与聚合物纳米颗粒复合物暴露于小鼠Nemeth-Kellner淋巴瘤细胞的生物能量特性

The Ukrainian Biochemical Journal Pub Date : 2023-02-23 DOI: 10.15407/ubj94.06.030

M. Ilkiv, Ya. R. Shalai, H. Mazur, B. O. Manko, B. V. Manko, Y. Ostapiuk, N. Mitina, A. Zaichenko, A. Babsky

{"title":"Bioenergetic characteristics of the murine Nemeth-Kellner lymphoma cells exposed to thiazole derivative in complex with polymeric nanoparticles","authors":"M. Ilkiv, Ya. R. Shalai, H. Mazur, B. O. Manko, B. V. Manko, Y. Ostapiuk, N. Mitina, A. Zaichenko, A. Babsky","doi":"10.15407/ubj94.06.030","DOIUrl":"https://doi.org/10.15407/ubj94.06.030","url":null,"abstract":"The development of a new anticancer drugs targeted at energy metabolism of tumor cells is a promising approach for cancer treatment. The aim of our study was to investigate the action of thiazole derivative N-(5-benzyl-1,3-thiazol-2-yl)-3,5-dimethyl-1-benzofuran-2-carboxamide (BF1) and its complex with PEG based polymeric nanoparticle (PEG-PN) on respiration and mitochondrial membrane potential in murine NK/Ly tumor cells. The rate of oxygen uptake in NK/Ly cells was recorded by a polarographic method using a Clark electrode. The mitochondrial potential relative values were registered using fluorescence TMRM dye. No changes in glucose-fuelled basal respiration or maximal FCCP-stimulated respiration was detected after 15-min incubation of cells with BF1 (10 µM), PEG-PN or BF1 + PEG-PN complex Fluorescent microscopy data showed that BF1 or PEG-PN separately had no effect on the value of mitochondrial membrane potential, while BF1 + PEG-PN complex caused a significant decrease in mitochondrial membrane potential, indicating on the decrease of NK/Ly cells viability. Keywords: cell respiration, mitochondrial membrane potential, NK/Ly tumor cells, PEG, polymeric nanoparticles, thiazole derivative","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78859058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0