V. N. Gerasimov, E. M. Aslanyan, A. Tyryshkina, N. A. Leontyeva
{"title":"OBTAINING STANDARD HELMINTH EGG CULTURES FOR STUDYING THE OVICIDAL EFFICACY OF CHEMICALS","authors":"V. N. Gerasimov, E. M. Aslanyan, A. Tyryshkina, N. A. Leontyeva","doi":"10.31016/978-5-6048555-6-0.2023.24.146-150","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.146-150","url":null,"abstract":"In the prevention of parasitic diseases, an important place is occupied by the search \u0000for new highly effective chemicals for the disinfection of environmental objects. When \u0000selecting disinfectants, helminth eggs are often used as test objects. The availability \u0000of high-quality and standard test material contributes greatly to successful research. \u0000However, publications devoted to the methodological side of this problem are clearly \u0000not enough. Ascaridata eggs (Ascaris suum, Toxocara canis, etc.) are the most resistant \u0000to chemicals, so testing on them allows us to identify the most effective means for \u0000the purposes of disinfection. Special attention should be paid to such an object as \u0000Enterobius vermicularis, the causative agent of enterobiasis, the most common of \u0000helminth infections in humans. This paper describes methods for obtaining standard \u0000egg cultures of such test objects as A. suum and E. vermicularis. A more standard A. \u0000suum egg culture is facilitated by material collected only from females with high egg \u0000viability, as well as the destruction of egg conglomerates in a suspension using sodium \u0000hypochlorite. Standardization of the E. vermicularis egg culture is only achieved by \u0000taking samples containing healthy viable eggs after the preview.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78778726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"DIROFILARIASIS DIAGNOSTICS IN DOGS","authors":"V. Kravchenko, G. Kravchenko, G. Itin","doi":"10.31016/978-5-6048555-6-0.2023.24.225-228","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.225-228","url":null,"abstract":"As a result of our own long-term research, a comparative analysis of clinical \u0000signs, and laboratory, immunological and instrumental methods for diagnosing \u0000dirofilariasis of dogs in the North-Western Caucasus was carried out. As a result \u0000of the study, it was found that only a comprehensive diagnosis performed in stages \u0000allows us not only to diagnose most accurately both mature nematodes and their \u0000microfilariae but also to significantly reduce the economic component. Therefore, \u0000when diagnosing dirofilariasis in dogs, it is necessary to take into account only the \u0000most specific clinical signs. Laboratory methods should combine both simple express \u0000methods and more complex, time-consuming ones. When choosing immunological \u0000test systems, it is necessary to take into account not only their economic feasibility, \u0000but also the qualitative component, since the main principle of test system operation \u0000is the reaction to Dirofilaria antigens contained in the blood of an infected dog. They \u0000can be used for both individual and group research. When choosing instrumental \u0000diagnostic methods such as CT, MRI, ultrasound, etc., which are aimed at detecting \u0000sexually mature nematodes, and due to their high cost and complexity, it is necessary \u0000to take into account their expediency based on predicting the disease outcome.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75856865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"EPIZOOTIC SITUATION ON THE MAIN GASTROINTESTINAL HELMINTH INFECTIONS OF HORSES IN THE ALTAI REPUBLIC","authors":"V. A. Marchenko, E. A. Efremova, M. A. Smertina","doi":"10.31016/978-5-6048555-6-0.2023.24.290-294","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.290-294","url":null,"abstract":"One of the problems of herd horse breeding of the Altai Mountains is significant \u0000spread of helminth infections. The purpose of this research is to characterize the \u0000epizootic situation on intestinal helminthiasis in the main horse-breeding areas \u0000of the Altai Republic. Coproovoscopic studies of horse feces from 8 districts \u0000(Mayminsky, Shebalinsky, Ongudaisky, Chemalsky, Ust-Koksinsky, Kosh-Agachsky \u0000and Ulagansky) were carried out for eggs of intestinal helminths by the KotelnikovKhrenov flotation method with the counting of eggs according to the VIGIS method. \u0000In total, 989 fecal samples from adult New Altai horses were examined. According to \u0000the results of coprological examinations, indicators of infection were derived: IP, %, \u0000the infection prevalence, the proportion of infected animals; mII is the arithmetic \u0000mean of the number of eggs in 1 gram of feces (egg/g f.) per one examined animal. In \u0000the horses of the Altai Mountains, pathogens of gastrointestinal helminth infections belonging to 5 suborders were recorded: Strongylata, Ascaridata, Anoplocephalata, \u0000Oxyurata and Rabditata. To a greater extent, the horses were infected with \u0000gastrointestinal Strongylata with an IP level of 76.2% and an egg count of 293 \u0000specimens in a gram of feces, and to a lesser extent with Parascarids (IP 11.2%) \u0000and Anoplocephalida (IP 12.3%). The animals in the Chemalsky District were most \u0000intensively infected with helminths (IP 96.3%), significantly lower than the horses \u0000in the Kosh-Agachsky District (IP 62.3%).","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83850218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"MUSEUM OF THE DEPARTMENT OF PARASITOLOGY AND VETERINARY AND SANITARY EXPERTISE NAMED AFTER K. I. SKRYABIN","authors":"F. Vasilevich, S. A. Shemyakova, N. Esaulova","doi":"10.31016/978-5-6048555-6-0.2023.24.138-141","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.138-141","url":null,"abstract":"The paper describes the structure of the Museum of Parasitology located on the \u0000basis of the Department of Parasitology and Veterinary and Sanitary Expertise of \u0000the Federal State Budgetary Educational Institution of Higher Education \"Moscow \u0000State Academy of Veterinary Medicine and Biotechnology – MBA named after \u0000K. I. Skryabin\", which was organized by Konstantin Ivanovich Skryabin. The first \u0000gross specimens were provided by him from his own collection. Later, together with \u0000his students, K. I. Skryabin replenished the collection with helminths brought by \u0000them from numerous expeditions to various regions of the Soviet Union. In 2017, \u0000the Museum acquired a new \"face\", major repairs were made, and thematic stands \u0000were allocated: helminthiasis of horses, cattle and small cattle, pigs, fish, birds, dogs \u0000and cats. There are exhibits showing ticks and insects that parasitize animals. Eighty zoonotic helminth infections are allocated to a separate stand, of which 26 are \u0000caused by trematodes, 17 by cestodes, 30 by nematodes, 3 by Acanthocephali, and 4 \u0000by pentastomes. A separate part of the Museum's permanent exhibition is a section \u0000dedicated to Academician K. I. Skryabin which presents original photographs, \u0000albums, personal belongings of the Academician, letters, postcards addressed to \u0000K. I. Skryabin, lectures and dissertations of the Academician, as well as technical \u0000means by which the material was studied (microscopes, magnifying glasses), and \u0000numerous works of K. I. Skryabin.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88335300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"GASTROINTESTINAL STRONGYLATOSIS OF HORSES","authors":"R. Timerbaeva, A. R. Shageeva, G. Frolov","doi":"10.31016/978-5-6048555-6-0.2023.24.470-474","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.470-474","url":null,"abstract":"The epizootic situation was studied on gastrointestinal strongylatosis of horses in \u0000Sosna, LLC of the Baltasinsky District, the Republic of Tatarstan. The study objects \u0000were horses of different age groups, breeds, and sexes, spontaneously infected by \u0000nematodes. Coproscopic studies were carried out by the modified helminthovoscopic \u0000Kotelnikov-Khrenov method with ammonium nitrate and the helmintholarvoscopic \u0000Shilnikov method. The generic assignment of pathogens of gastrointestinal \u0000strongylatosis of horses was determined by cultivating nematode larvae belonging to \u0000the Strongylata suborder. As a result of the coproscopic studies, it was found that horses \u0000of different age groups were invaded by pathogens of gastrointestinal strongylatosis. \u0000In December 2021, 30 horses were tested for helminthiasis. The results of the studies \u0000showed that 20 out of 30 animals had gastrointestinal Strongylata infections with \u000066.7% extense-invasiveness, and the intense-invasiveness of 1 to 37 egg specimens in the field of view. The remaining 10 horses were free of helminths. The studies indicate \u0000that the examination of the horses in Sosna, LLC detected intestinal nematodiasis \u0000the causative agents of which were nematodes of the Strongylata suborder, the \u0000genus Triсhonema. Thus, widespread parasitic diseases of horses on the farm were \u0000gastrointestinal strongylatosis, in particular trichonematosis.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87188339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"EPIZOOTIC SITUATION ON THE CONTAMINATION OF ENVIRONMENTAL OBJECTS WITH INVASIVE ELEMENTS ON THE CATTLE FARM OF THE MOSCOW REGION","authors":"R. Safiullin, S. Shibitov, R. Safiullin","doi":"10.31016/978-5-6048555-6-0.2023.24.420-425","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.420-425","url":null,"abstract":"The study of the epizootic situation on the contamination of environmental objects \u0000with invasive elements on the livestock farm of the Moscow Region showed its \u0000contamination with parasitic protozoa oocysts and cysts, and helminth eggs and \u0000larvae which was directly affected by infection of the livestock with the above \u0000parasites and the quality of therapeutic and prophylactic and veterinary and sanitary \u0000measures. The objects of the external environment most contaminated with invasive \u0000elements of parasitic protozoa and helminths were the floor of stalls and passages \u0000in the premises for keeping animals. The stall walls and animal feeders were less \u0000contaminated with parasitic protozoa oocysts and cysts or helminth eggs or larvae. \u0000It should be noted that, the epizootic process for parasitic protozoa: Buxtonella, \u0000Cryptosporidium, Eimeria and Strongylata of the digestive tract, was permanent \u0000on the examined livestock farm of the Moscow Region, since all of its components \u0000were in place: the source of invasion was infected animals; transmission factors were \u0000environmental objects contaminated with invasive elements and susceptible animals, especially young animals. An analysis was performed of the plan of preventive antiepizootic measures of this farm in relation to parasitic diseases in terms of diagnostic \u0000tests for parasitosis, and therapeutic-and-prophylactic and veterinary-sanitary \u0000treatments.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90551760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"DEFINITIVE HOSTS OF ALARIA ALATA (TREMATODA, STRIGEIDIDA) IN NATURAL CONDITIONS OF THE CENTRAL BLACK EARTH REGION (VORONEZH AND LIPETSK REGIONS)","authors":"B. Romashov, N. Romashova","doi":"10.31016/978-5-6048555-6-0.2023.24.386-390","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.386-390","url":null,"abstract":"Currently in the Central Black Earth Region, an increase in cases of infection of wild \u0000and domestic carnivores with the trematode Alaria alata (Trematoda, Strigeidida) \u0000has been recorded. Helminthological materials were collected in the Voronezh \u0000Nature Reserve and in adjacent territories (Voronezh and Lipetsk Regions) for \u0000more than 20 years (2000–2022). More than 100 species of carnivorous mammals \u0000of the Canidae family were studied. A. alata maritae were recorded in four predator \u0000species: the wolf, fox, raccoon dog, and the domestic dog. The total infection rate in \u00004 Canidae species was 78.3%. The highest infection prevalence was registered in the \u0000wolf, 97.0%, and the raccoon dog, 100%, and slightly lower in the fox, 81.8%. For \u0000the domestic dog, the incidence of Alaria species was found at the level of 34.4%. \u0000In the study area, the fox was the most numerous predator. The fox had significant \u0000infection prevalence values and the highest relative abundance of maritae (infection \u0000intensity and abundance index). A fairly large sample of foxes was studied in adjacent \u0000territories that were mainly agricultural lands. Relatively high levels of infection were \u0000detected: the IP 75.9%; the II 162.0; and the AI 122.9. The fox plays the leading role \u0000in the circulation of alariosis in the Central Black Earth Region.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90613980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"CULTURE OF EIMERIA SPP. OOCYSTS OF TURKEY POULTS AND THEIR SPECIES IDENTIFICATION","authors":"R. Safiullin, E. Chalysheva","doi":"10.31016/978-5-6048555-6-0.2023.24.414-419","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.414-419","url":null,"abstract":"In our country, in recent years, much attention has been paid to the development of \u0000poultry meat production, especially turkey breeding. In the conditions of industrial \u0000turkey breeding, when a large number of poultry is kept in a limited area, there is a \u0000high risk of parasitic diseases, one of which is eimeriosis. Knowledge of the species \u0000composition of Eimeria on a particular poultry farm is of great practical importance \u0000for the reasonable development of effective methods to control invasion and to \u0000monitor Eimeria resistance to the drugs used. Eimeria species were identified after \u0000the end of sporulation. To assess the course of sporulation of Eimeria oocysts during \u0000their cultivation, at least 500 oocysts were examined from each Petri dish every six \u0000hours under a high magnification microscope (x400) paying special attention to \u0000their morphology. When examining and studying litter samples 24 hours after they \u0000were put on cultivation, sporulated Eimeria oocysts of turkeys were detected in all six \u0000dishes in 37.8% to 60.6% of those examined, and the average rate was 51.6%. At 48 \u0000hours after the start of cultivation, the average Eimeria sporulation rate was 83.4%. \u0000The results of species identification of Eimeria oocysts showed that the following \u0000Eimeria species were found in young turkeys on the poultry farm of the Tula Region: \u0000Eimeria meleagrimitis (60.0%), E. gallopavonis (25.0%), E. meleagridis (10.0%), and \u0000E. adenoides (5.0%).","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"09 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86022187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"THE STUDY OF EFFECTS OF АMINOTONE ON HEMATOLOGICAL AND BIOCHEMICAL STATUS OF DOGS IN BABESIOSIS THERAPY","authors":"I. D. Shelyakin, S. N. Semenov, L. V. Cheskidova","doi":"10.31016/978-5-6048555-6-0.2023.24.532-537","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.532-537","url":null,"abstract":"When dogs are invaded with Babesia, metabolic and detoxification processes \u0000are disrupted. In this regard, the therapeutic regimen should include drugs that \u0000contribute to the normalization of metabolism, and maintenance of liver and kidney \u0000function. The purpose of our research was to study the effects of Аminotone on the \u0000hematological and biochemical status of dogs during the treatment of babesiosis. For \u0000this purpose, Forticarb was administered as an etiological agent to the animals of the \u0000control group, and Аminotone was additionally administered to the experimental \u0000dogs. Before the experiment and two weeks after the beginning of the treatment, blood \u0000was taken from the dogs for hematological and biochemical studies. It was found that \u0000the control and experimental animals showed a decrease in leukocytes, ESR, ALT and AST activity, urea, and creatinine and bilirubin concentrations after the recovery. \u0000At the same time, an increase in erythrocytes, hematocrit, mean corpuscular volume \u0000and hemoglobin was recorded. Аminotone provided more intensive changes in the \u0000dogs’ biochemical and hematological status: reduced inflammatory reaction, toxic \u0000load on the liver and kidneys, and stimulated hematopoiesis, versus the control. \u0000Thus, the drug can be recommended for the combined therapy of canine babesiosis.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73511074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. E. Mazur, I. Kutyrev, T. Sidorova, L. Sukhanova
{"title":"TRANSCRIPTOME ANALYSIS OF INTESTINES OF THE BAIKAL OMUL (LAKE BAIKAL, EASTERN SIBERIA)","authors":"O. E. Mazur, I. Kutyrev, T. Sidorova, L. Sukhanova","doi":"10.31016/978-5-6048555-6-0.2023.24.268-274","DOIUrl":"https://doi.org/10.31016/978-5-6048555-6-0.2023.24.268-274","url":null,"abstract":"New data were presented for the first time on the intestinal transcriptome of the \u0000intestines (posterior section) of the Baikal omul Coregonus migratorius Georgi, 1775 \u0000(Salmoniformes: Coregonidae) belonging to the deep water bottom morphological \u0000group (Lake Baikal) infected with parasites of various systematic groups: cestodes \u0000Dibothriocephalus dendriticus, Proteocephalus longicollis, Eubothrium crassum, and \u0000nematode Contracaecum osculatum baicalensis. Sequencing of the transcriptomics \u0000libraries was performed on an Illumina NextSeq550 sequencer using the NextSeq® \u0000550 High Output Kit v2. Based on the obtained data, de-novo transcriptome assembly was performed. Conserved domains and their associated Gene Ontology \u0000annotations were predicted using Blast2Go. As a result, it was found that in the posterior section of the intestine enriched with lymphoid tissue (GALT), expression \u0000of functional proteins was observed that were primarily associated with enzymatic \u0000activity, with the development of specialized tissues, with cellular, metabolic and \u0000secretory processes. It should be noted that a significant proportion of gene ontology \u0000terms is associated with the functioning of the immune system, and with the cellular \u0000response to stress, which, under conditions of sensitization by the metabolic end \u0000products of helminths and their traumatic and antigenic effects, is quite understandable. The identified transcriptome may provide new information for understanding \u0000the functions of lymphoid organs in salmonids with parasitosis.","PeriodicalId":22969,"journal":{"name":"THEORY AND PRACTICE OF PARASITIC DISEASE CONTROL","volume":"66 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88983221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}