火鸡艾美耳球虫卵囊的培养及其种类鉴定

R. Safiullin, E. Chalysheva
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引用次数: 0

摘要

近年来,我国非常重视禽肉生产的发展,特别是火鸡的养殖。在工业化火鸡养殖的条件下,当大量家禽在有限的区域内饲养时,存在很高的寄生虫病风险,其中之一是艾默里病。了解特定家禽养殖场艾美耳球虫的种类组成,对于合理制定有效的方法来控制入侵和监测艾美耳球虫对所使用药物的耐药性具有重要的现实意义。在产孢结束后鉴定艾美耳球虫种。为了评估艾美耳球虫卵囊在培养过程中的产孢过程,在高倍显微镜(x400)下每6小时从每个培养皿中检测至少500个卵囊,并特别注意其形态。在培养24小时后对鸡窝样品进行检查和研究,6个培养皿中均检出了火鸡的孢子艾美耳球虫卵囊,检出率为37.8% ~ 60.6%,平均检出率为51.6%。培养开始后48 h,艾美耳球虫平均产孢率为83.4%。对图拉地区养鸡场雏鸡卵囊的艾美耳亚种鉴定结果表明,在雏鸡中发现的艾美耳亚种为:肉鸡艾美耳亚种(60.0%)、加洛帕翁艾美耳亚种(25.0%)、肉鸡艾美耳亚种(10.0%)和腺状艾美耳亚种(5.0%)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
CULTURE OF EIMERIA SPP. OOCYSTS OF TURKEY POULTS AND THEIR SPECIES IDENTIFICATION
In our country, in recent years, much attention has been paid to the development of poultry meat production, especially turkey breeding. In the conditions of industrial turkey breeding, when a large number of poultry is kept in a limited area, there is a high risk of parasitic diseases, one of which is eimeriosis. Knowledge of the species composition of Eimeria on a particular poultry farm is of great practical importance for the reasonable development of effective methods to control invasion and to monitor Eimeria resistance to the drugs used. Eimeria species were identified after the end of sporulation. To assess the course of sporulation of Eimeria oocysts during their cultivation, at least 500 oocysts were examined from each Petri dish every six hours under a high magnification microscope (x400) paying special attention to their morphology. When examining and studying litter samples 24 hours after they were put on cultivation, sporulated Eimeria oocysts of turkeys were detected in all six dishes in 37.8% to 60.6% of those examined, and the average rate was 51.6%. At 48 hours after the start of cultivation, the average Eimeria sporulation rate was 83.4%. The results of species identification of Eimeria oocysts showed that the following Eimeria species were found in young turkeys on the poultry farm of the Tula Region: Eimeria meleagrimitis (60.0%), E. gallopavonis (25.0%), E. meleagridis (10.0%), and E. adenoides (5.0%).
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