Theoretical and Applied Genetics最新文献

{"title":"The COBRA-Like9 from Glycine tabacina enhanced salt tolerance in cultivated soybean.","authors":"Sawaira Jadoon, Zhengwei Zhang, Menghui Tan, Shanshan Zhang, Dan Zhang, Yijie Chen, Yajun Xiong, Huan Yu, Xin Su, Fan Zhang, Jun Wang, Lijuan Qiu","doi":"10.1007/s00122-026-05331-5","DOIUrl":"10.1007/s00122-026-05331-5","url":null,"abstract":"<p><p>Soybean is one of the major sources of high-quality protein and oil and plays a crucial role in ensuring nutritional and economic security. The rapidly increasing population, soybean supply-demand imbalance, and yield losses due to soil salinity collectively necessitate the development of salt-tolerant cultivars. Perennial wild Glycine species harbor-rich genetic diversity and constitute a reservoir of stress-tolerant genes. This study systematically characterized the COBL gene family in the salt-resilient Glycine tabacina (Labill.) Benth., a wild soybean that tolerates 500 mM NaCl with 100% survival compared with only 5% in cultivated soybean. We identified 44 GtCOBL genes nearly double the number in cultivated soybean driven by tetraploidization and tandem duplication events. We further identified a novel salt-induced gene, GtCOBRA-Like9 (GtCOBL9), from G. tabacina, which encodes a plasma membrane protein possessing a cellulose-binding motif and a GPI anchor, supporting its potential role in cell wall modification. Functional validation revealed that overexpression of GtCOBL9 in cultivated soybean-enhanced seedling salt tolerance. Elevated cellulose content in the roots of GtCOBL9-OE lines suggest that GtCOBL9 may function in cell wall modeling. Notably, we uncovered a novel interaction between GtCOBL9 and the ion-transport regulators GmAKT1 and GmCAX1. These genes were upregulated in GtCOBL9 overexpression lines and roots of GtCOBL9-OE-1 showed higher K⁺ retention and increased Na⁺ efflux while no significant difference in total ion content between GtCOBL9-OE lines and wildtype, suggesting that GtCOBL9 is associated with ion flux dynamics rather than bulk accumulation. Additionally, GtCOBL9-OE lines exhibited reduced reactive oxygen species accumulation, lower expression of GmRbohB2 and GmAOX1, higher catalase (CAT) activity, and increased proline content, suggesting a role in oxidative stress mitigation. Taken together, this study identifies a novel GtCOBL9 that may confers salt tolerance to cultivated soybean through cell wall modification, ionic and oxidative adjustments, however underlying molecular mechanisms require further investigations.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"139 9","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148798045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The functions of TaLAD1-A1, Rht8, and Rht-D1b in regulating leaf angle in wheat.","authors":"Chaojie Wang, Lili Zhang, Qiushi Wang, Huijun Guo, Hongchun Xiong, Jiayu Gu, Linshu Zhao, Chengshe Wang, Shoucai Ma, Gaisheng Zhang, Yongdun Xie, Luxiang Liu","doi":"10.1007/s00122-026-05351-1","DOIUrl":"10.1007/s00122-026-05351-1","url":null,"abstract":"<p><strong>Key message: </strong>A genotypic pattern for breeding wheat with ideal plant architecture. Breeding wheat with reduced leaf angle represents a promising strategy for developing lines tolerant to high planting density, mitigating the adverse effects of shade avoidance and thus increasing yield potential. However, the molecular mechanisms underlying the reduced leaf angle phenotype remain poorly understood. In this study, a wheat mutant with a reduced leaf angle, designated lad1 and exhibiting impaired sensitivity to brassinosteroids (BRs), was isolated from an EMS-induced mutant library. Phenotypic analysis revealed that the reduced leaf angle in lad1 plants is due to a decrease in the size of lamina joint cells. Genetic mapping and cloning identified Talad1-A1, a single recessive nuclear gene located on chromosome 2A, is responsible for the variation in leaf angle. Furthermore, Rht8 and Rht-D1b have been shown to contribute significantly to leaf angle regulation. These findings deepen our understanding of the genetic and molecular mechanisms controlling leaf angle in wheat and provide a potential molecular selection strategy for the developing elite wheat varieties adapted to high-density planting.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"139 9","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148798076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide linkage and association mapping identified a novel candidate gene, TaGLK-A1, underlying fructan content in wheat grain.","authors":"Zhankui Zeng, Yue Zhao, Junge Bi, Qunxiang Yan, Wei Zhang, Feng Chen, Chunping Wang","doi":"10.1007/s00122-026-05345-z","DOIUrl":"10.1007/s00122-026-05345-z","url":null,"abstract":"<p><strong>Key message: </strong>A nuclear-localized G2-like transcription factor, TaGLK-A1 (TraesCS7A02G539600), was identified as a strong candidate gene for grain fructan content in wheat using GWAS, linkage mapping and transcriptome analysis. Fructans, a type of natural polysaccharides or oligosaccharides polymerized from fructose molecules, play important physiological roles in crops and confer great health benefits to humans. In this study, we detected 78 stable loci associated with fructan content across all 21 wheat chromosomes across three environments and in the BLUE, explaining 4.4-10.0% of the phenotypic variance by a genome-wide association study (GWAS) using 243 wheat cultivars and advanced lines genotyped with the Axiom wheat 660 K single-nucleotide polymorphism (SNP) array. Among these, a significant locus of fructan content was localized in a 4.02 Mb interval (713.87-717.89 Mb) on chromosome 7AL, designated QGfc.hkd-7A. QGfc.hkd-7A was validated in 164 recombinant inbred lines (RILs) from a cross between Avocet and Chilero in five environments using diversity array technology (DArT), which was tightly linked to DArT markers SNP2260399, 3575400, SNP3028492, SNP100415949, 3533474 and 100091413, accounting for 16.4-24.6% of the total phenotypic variance in the RIL population. Favorable allele of QGfc.hkd-7A significantly increased grain fructan content by 10.5% (P < 0.01) compared with lines carrying unfavorable alleles. To further validate QGfc.hkd-7A, we generated differential gene expression profiles using RNA-seq for high-fructan (AC6012) and low-fructan (AC6361) lines from the Avocet/Chilero population. TaGLK-A1 (TraesCS7A02G539600), a differential expressed gene (DEG), was subsequently cloned and sequenced from both genotypes. Sequence comparison of the full-length TaGLK-A1 cDNA (1545 bp) revealed a single SNP at position 109 causing a proline (CCA) to threonine substitution between Avocet and Chilero. Subcellular localization analysis confirmed that the G2-like transcription factor encoded by TaGLK-A1 localizes to the nucleus. Transactivation assays revealed that it lacks intrinsic transcriptional activation activity, suggesting it may function as a transcriptional repressor or require cofactors for regulatory function. These results provide a novel and strong candidate gene for fructan content and a forward genetics strategy for gene discovery in molecular breeding.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"139 9","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13490229/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148798120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fine mapping and candidate gene analysis of tassel glume closure1 (tgc1) gene in maize (Zea mays L.).","authors":"Ziwen Shi, Jinmiao Qin, Sheng Zhang, Yujiao Gao, Qing He, Hongyang Yi, Tingzhao Rong, Moju Cao","doi":"10.1007/s00122-026-05353-z","DOIUrl":"10.1007/s00122-026-05353-z","url":null,"abstract":"<p><strong>Key message: </strong>The tassel glume closure1 (tgc1) mutant was identified and mapped, and ZmKAO1, which encodes an ent-kaurenoic acid oxidase, is the key candidate gene. Tassel glume timely opening is a key trait that influences cross-pollination and hybrid breeding. However, in maize (Zea mays L.), the related genes regulating this process remain largely unexplored. Here, we identified tassel glume closure1 (tgc1), a recessive mutant that exhibits tassel glume closure, anther non-extrusion, and no pollen shedding at the adult-plant stage. Anatomical and scanning electron microscopy (SEM) observations revealed that the tgc1 mutant phenotype is caused by the hindrance of filament elongation and lodicule swelling. We mapped the tgc1 locus and determined that Zm00001eb379120, which encodes ent-kaurenoic acid oxidase1 (ZmKAO1), is the key candidate gene of tgc1. Transcriptome analysis revealed that gibberellin (GA) biosynthesis was disrupted in tgc1 tassel, and exogenous application of GA₃ can fully rescue the tgc1 mutant phenotype. Based on the tgc1 mutant, we propose the novel Pseudo-Genic Male Sterility (GMS) Two-Line (PGTL) system for maize hybrid seed production. These findings enrich our understanding of ZmKAO1 function in tassel glume timely opening and provide a new germplasm resource and a promising technical approach for maize commercial hybrid seed production.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"139 9","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148798133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimating on-farm genotypic performance and variability using ranking data.","authors":"Hugo Dorado-Betancourt, Fred van Eeuwijk, Jacob van Etten, Julian Ramirez-Villegas, Kaûe de Sousa, Happy Daudi, Michael Ndegwa, Thiago Mendes, Joost van Heerwaarden","doi":"10.1007/s00122-026-05319-1","DOIUrl":"10.1007/s00122-026-05319-1","url":null,"abstract":"<p><strong>Key message: </strong>Our scalable two-step method estimates genotypic performance and genetic parameters from ranking data, producing reliable results comparable to quantitative analyses, enabling the integration of ranking data into breeding pipelines. Plant breeding research has chiefly relied on on-station experiments to evaluate varietal performance. Nevertheless, these trials often fail to represent on-farm growing conditions and farmers' preferences, potentially leading to poorly defined breeding targets. Recent work has demonstrated the potential of using on-farm verification trials combined with ranking data to support farmers in evaluating varieties while providing information that is representative of farmers' needs. Despite this potential, scalable methods for quantifying genetic differences and assessing the strength of the genetic signal in such trials remain limited. Here, we present a two-step procedure for analyzing trials based on ranking data, allowing the estimation of genetic parameters. The approach follows a common strategy in quantitative genetics, in which parameters are estimated from tables of genotypic means and their variances. In our framework, these estimates are obtained from Thurstonian and/or Plackett-Luce models, which treat rankings as observations of an underlying continuous trait associated with genotypic performance. Using simulated data, we showed that genotypic mean estimates derived from ranking analyses are linearly related to those obtained from quantitative trait analyses and that their variances adequately capture estimation uncertainty. We further demonstrated that incorporating these estimates and their variances into a second-step mixed-effects model yields accurate estimates of variance components. Analyses of groundnut, maize, and sweetpotato datasets confirmed the applicability of the approach and showed that ranking data can provide reliable estimates of genetic parameters. We argue that this framework can be scaled to obtain genotypic performance estimates from multi-trial on-farm data.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"139 9","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13486096/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148798088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Haplotype-based nested association mapping for net form net blotch response in Australian barley.","authors":"Muhammad Nadeem, Dan Liu, Lisle Snyman, Lee Hickey, Eric Dinglasan","doi":"10.1007/s00122-026-05328-0","DOIUrl":"10.1007/s00122-026-05328-0","url":null,"abstract":"<p><strong>Key message: </strong>Haplotype-based nested association mapping conducted in Australian barley identified 30 haploblocks for resistance/susceptibility to net form of net blotch. Stacking multiple resistance haplotypes significantly reduced disease severity. Net form net blotch (NFNB), caused by Pyrenophora teres f. teres, is a major fungal disease affecting barley, leading to significant yield losses globally. Improving the sustainability of barley production requires identifying genetic sources that confer effective resistance across genetic backgrounds and diverse environments. Considerable progress has been made through mapping studies conducted internationally, but many have largely focused on unadapted germplasm such as landraces or used foreign pathotypes. To support breeding outcomes in Australia, characterization of resistance alleles that are effective against local pathotypes is critical, along with investigating resistances that may already be present in breeding germplasm as this could reduce the time to deployment of resistant cultivars. In this study, we applied the local genomic estimated breeding value approach to perform haplotype mapping using a multi-reference parent nested association mapping (MR-NAM) population developed using resistant breeding lines as donor parents. The population was evaluated across three seasons in Queensland to investigate the genetic architecture of NFNB resistance and genotype-by-environment interactions. We identified 30 haploblocks, with seven representing novel genomic regions not previously associated with NFNB resistance. Three haploblocks on chromosomes 2H, 6H, and 7H were consistently associated with resistance across all environments, suggesting that they likely harbor stable resistance loci. Haplotype stacking analysis demonstrated that combining multiple resistance haplotypes significantly reduced disease severity, with lines carrying 6-10 resistance haplotypes showing progressive improvements in NFNB resistance. The distribution and effectiveness of these key haplotypes across environments, and in diverse genetic backgrounds adapted to Australia (Commander, Compass and La Trobe), highlight the potential to harness them in ongoing pre-breeding and breeding programs.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"139 9","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13486095/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148798056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transposable elements and their epigenetic modifications mediate gene expression and subgenome dominance in Brassica carinata.","authors":"Guoqing Li, Shici Zhu, Jiangfeng Li, Fanzhe Kong, Jianbo Wang","doi":"10.1007/s00122-026-05349-9","DOIUrl":"10.1007/s00122-026-05349-9","url":null,"abstract":"<p><strong>Key message: </strong>Subgenome-specific TE expansion and multilayered epigenetic modifications synergistically and antagonistically mediate gene expression, shaping subgenome dominance in B. carinata. Transposable elements (TEs) are major components of plant genomes and play pivotal roles in subgenome differentiation and gene expression regulation in allopolyploid plants; however, the regulatory effects mediated by epigenetic modifications of TEs remain poorly understood. Here, using allotetraploid Brassica carinata (B_cB_cC_cC_c genome) as a model, we systematically investigated the regulatory roles of TE on gene expression by inserting genes, capturing genes, and multiple epigenetic modifications (DNA methylation and histone modifications) within TE regions. Distinct TE superfamilies exhibited differential expansion and dynamic evolutionary patterns between the B_c and C_c subgenomes, LTR retrotransposons expanded more prominently in the B_c subgenome, whereas TIR transposons exhibited stronger expansion in the C_c subgenome, contributing to subgenome structural divergence. TE insertions and TE-mediated gene capture regulate the expression of both neighboring and distal genes through synergistic and antagonistic interactions among multiple epigenetic modifications. Notably, the dominant B_c subgenome exhibits stronger H3K9me2 enrichment and lower DNA methylation in TE regions, while TEs in the C_c subgenome display higher DNA methylation and more diverse histone modifications. Collectively, TEs and their multilayered epigenetic modifications jointly shape the asymmetric subgenome expression landscape of B. carinata, providing new insights into the establishment of subgenome dominance in allopolyploid plants.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"139 9","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148798092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antagonistic pleiotropy at the stem solidness 1 locus balances wheat stem architecture and yield components under terminal drought.","authors":"Simeon Ntawuguranayo, Roy Sadeh, Hanan Sela, Ittai Herrmann, Zvi Peleg, Roi Ben-David","doi":"10.1007/s00122-026-05330-6","DOIUrl":"10.1007/s00122-026-05330-6","url":null,"abstract":"<p><strong>Key message: </strong>Stem structural investment enhances grain size and yield under drought, challenging the traditional view of vegetative-reproductive trade-offs. Antagonistic pleiotropy at the SSt1 locus reveals a genetic constraint between stem diameter and solidness. Incorporating genotype-by-environment into genomic selection models improves prediction accuracy by 46.5%. Terminal drought is the primary environmental constraint on wheat productivity in the Mediterranean basin, a challenge intensifying under climate change. While stem biomass investment is known to promote grain-filling under stress, its underlying genetic architecture remains poorly understood. We evaluated a bread wheat diversity panel (n = 295; WheatMore) across three Mediterranean environments: well-watered (635 mm), terminal drought (228 mm), and rain-fed (468 mm). Our objectives were to characterize the phenotypic and genetic trade-offs between stem traits and yield components, identify associated genomic regions, and implement multi-environment genomic selection models. Increased investment in stem structural biomass, specifically dry weight, diameter, and peduncle length, was positively correlated with grain size and yield per spike, particularly under terminal drought. These results suggest that robust stem architecture enhances source strength without penalizing yield, as increased grain weight compensates for the biomass investment. Genome-wide association identified 80 significant SNPs and 77 gene hits, including 14 stable, 61 environment-specific, and 21 pleiotropic loci. Notably, pleiotropic regulation at the stem solidness 1 locus (SSt1) on chromosome 3B revealed a genetic trade-off between stem diameter and solidness mediated by antagonistic alleles, highlighting a constraint for simultaneous optimization. Multi-environment genomic prediction incorporating genotype-by-environment interaction outperformed single-environment models, increasing prediction accuracy by 46.5%. These findings provide breeders with markers and predictive tools to develop wheat stem ideotypes to optimize climate adaptability.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"139 9","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13478034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148762323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epigenetic regulation and ROS signaling in plant stress adaptation: an integrated framework.","authors":"Nadeem Bhanbhro, Qadir Bakhsh, Shengdixin Shi, Jia-Jun Ma, Mian Fazli Basit, Hong-Jin Wang, Zhao Yun, Ali Murad Jakhar, Uzair Ullah, Abdullah Shalmani, Jing-Jing Wen, Kun-Ming Chen","doi":"10.1007/s00122-026-05344-0","DOIUrl":"10.1007/s00122-026-05344-0","url":null,"abstract":"<p><p>Plants respond to environmental stress by integrating epigenetic regulation with reactive oxygen species (ROS) signaling. This review examines the bidirectional interactions between epigenetic mechanisms and ROS homeostasis in plant stress adaptation, with a particular emphasis on drought resistance. Four epigenetic mechanisms, including histone modifications, DNA methylation, chromatin remodeling, and non-coding RNAs, control the expression of ROS-related genes, while in turn, ROS also alter chromatin structure and DNA methylation patterns. We propose that these interactions take the form of dynamic regulatory networks rather than one-way pathways, where changes in DNA methyltransferases and demethylation factors via ROS create reversible epigenetic states. This bilateral regulation can establish self-strengthening circuits that are capable of providing immediate responses to recurring stress. However, there is still a significant lack of knowledge, including the inconsistent reproducibility of stress priming protocols in studies, the incomplete understanding of how global oxidative stress induces epigenetic changes at specific sites, and the limited capacity for transgenerational transmission of stress-induced modifications. We review the evidence for epigenetic memory in plant stress responses, distinguish recurring adaptive plasticity from random variation, and highlight key mechanistic research directions for developing seasonally resilient crops through targeted epigenetic strategies.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"139 9","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148725227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BrDDB1A promoted flowering via age pathway in Chinese cabbage.","authors":"Yuanzhi Bai, Meidi Zhang, Guoliang Yang, Mingxin Chen, Nan Wang, Hui Feng","doi":"10.1007/s00122-026-05343-1","DOIUrl":"10.1007/s00122-026-05343-1","url":null,"abstract":"<p><p>The transition from vegetative to reproductive growth, marked by flowering, is a pivotal developmental switch in plants, whereby the age pathway serves as an important regulatory mechanism governing this process. In this study, we screened a late-flowering mutant from EMS mutagenesis in Chinese cabbage. The late-flowering phenotype was unaffected by photoperiod, vernalization and GA₃ treatments, and obvious changes occurred at the transcriptional levels of age flowering pathway-related genes, which indicated that the mutant trait was mediated by the age pathway. Causal gene BrDDB1A, involved in UV-induced DNA damage recognition and repair, was identified via MutMap and KASP techniques, and its function was verified by sequencing analysis of two allelic mutants. BrDDB1A was located in the nucleus. UV-B delayed the flowering time of the WT but not that of the mutant, suggesting BrDDB1A was involved in UV-B-mediated flowering delay of Chinese cabbage.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"139 9","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148722451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}