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The Pakistan Veterinary Journal最新文献

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Actinobacillus seminis Secretes a Metalloprotease That Degrades Bovine Fibrinogen and IgG 半放线杆菌分泌一种金属蛋白酶降解牛纤维蛋白原和IgG
The Pakistan Veterinary Journal Pub Date : 2023-04-01 DOI: 10.29261/pakvetj/2023.020
De la Cruz Montoya Aldo Hugo
{"title":"Actinobacillus seminis Secretes a Metalloprotease That Degrades Bovine Fibrinogen and IgG","authors":"De la Cruz Montoya Aldo Hugo","doi":"10.29261/pakvetj/2023.020","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.020","url":null,"abstract":"Actinobacillus seminis is a Gram-negative bacterium member of the Pasteurellaceae family. Even though it is part of the ovine microbiota it is also the causal agent of genital organs affections, particularly epididymitis and orchitis. Infections caused by this microorganism generate economic losses to the ovine industry due to impaired fertility and sterility in affected animals. Knowledge about virulence factors expressed by A. seminis is scarce. In the present work, we describe the expression of a metalloprotease secreted by A. seminis that can degrade bovine fibrinogen and immunoglobulin G and present homology with a carboxy-terminal protease from A. seminis. This metalloprotease presents an optimal activity at a pH between 6 and 7, is stable up to 60°C, inactive at higher temperatures, and completely inhibited by 30 mM EDTA. The expression of this proteolytic activity is controlled by temperature, calcium, and iron. Proteases degrading extracellular matrix components and molecules involved in the immune response could facilitate and improve host colonization and invasion by A. seminis","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88572235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Oxidative Stress, Antioxidant Enzymes, Genotoxicity and Histopathological Profile in Oreochromis niloticus Exposed to Lufenuron 氟虫腈对尼罗褐虾氧化应激、抗氧化酶、遗传毒性和组织病理学的影响
The Pakistan Veterinary Journal Pub Date : 2023-03-01 DOI: 10.29261/pakvetj/2023.012
Fatimah A Al-Saeed, S. Naz, M. Saeed, Riaz Hussain, S. Iqbal, Ahmad Manan Mustafa Chatha, Abdul Ghaffar, R. Akram
{"title":"Oxidative Stress, Antioxidant Enzymes, Genotoxicity and Histopathological Profile in Oreochromis niloticus Exposed to Lufenuron","authors":"Fatimah A Al-Saeed, S. Naz, M. Saeed, Riaz Hussain, S. Iqbal, Ahmad Manan Mustafa Chatha, Abdul Ghaffar, R. Akram","doi":"10.29261/pakvetj/2023.012","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.012","url":null,"abstract":"Pesticides especially insecticides are widely used in the field of agricultural development all over the world to increase crop production. Moreover, exposure to such compounds does not only influence the intended targets but also induces adverse impacts on a number of unintended targets in animals. This research aimed to determine the toxic impacts of lufenuron (an insecticide) on the health status of fish in terms of measurement of oxidative stress, antioxidant enzymes, DNA damage and histopathological changes in Nile tilapia (Oreochromis niloticus) exposed to different concentrations. For this purpose, Oreochromis niloticus fish, weighing about 75-80g were arbitrarily separated into four different groups and then exposed to lufenuron @ 0.7, 1.2 and 1.7µg/ L for 39 days. The results showed that the fish exposed to the pesticide had significant changes in oxidative stress, antioxidant profile in gills and percentile rate of DNA damage in different visceral organs including liver, kidney, and gills. Results of light microscopic investigations indicated different histological changes in liver (necrosis of hepatocytes, degeneration of hepatocytes, vacuolar degeneration and congestion), gills (necrosis of lamellar epithelial cells, telangiectasia, and atrophy of secondary lamellae), heart (congestion, necrosis of neurons, microgliosis and intracellular edema), brain (congestion, myofibrosis, neutrophilic and myocarditis) and kidneys (necrosis of renal tubules, widening of urinary space, necrosis of renal tubular epithelial cells). A significantly escalate in oxidative stress while lower quantity of antioxidant biomarkers was documented in experimental fish. The findings of this study suggest that long-term exposure to lufenuron has negative health effects via induction of DNA damage, increased oxidative stress, lowering of enzymatic antioxidants profile and histological lesions in visceral organs of Nile tilapita (Oreochromis niloticus).","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81630203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Drug Resistance and Genetic Relatedness of Escherichia coli from Mink in Northeast China 东北水貂大肠杆菌耐药性及遗传亲缘关系研究
The Pakistan Veterinary Journal Pub Date : 2023-01-01 DOI: 10.29261/pakvetj/2023.062
{"title":"Drug Resistance and Genetic Relatedness of Escherichia coli from Mink in Northeast China","authors":"","doi":"10.29261/pakvetj/2023.062","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.062","url":null,"abstract":"","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87733728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Histomorphometric Comparison of Canine and Feline Nictitating Membranes 犬和猫鼻甲膜的组织形态学比较
The Pakistan Veterinary Journal Pub Date : 2023-01-01 DOI: 10.29261/pakvetj/2023.042
{"title":"Histomorphometric Comparison of Canine and Feline Nictitating Membranes","authors":"","doi":"10.29261/pakvetj/2023.042","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.042","url":null,"abstract":"","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83924073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Effect of Xylanase and Phytase Supplementation Alone or in Combination in a Wheat-based Diet on Intestinal Morphology and Blood Profile in Broilers 小麦基础饲粮中单独或联合添加木聚糖酶和植酸酶对肉仔鸡肠道形态和血液特征的影响
The Pakistan Veterinary Journal Pub Date : 2023-01-01 DOI: 10.29261/pakvetj/2023.072
{"title":"The Effect of Xylanase and Phytase Supplementation Alone or in Combination in a Wheat-based Diet on Intestinal Morphology and Blood Profile in Broilers","authors":"","doi":"10.29261/pakvetj/2023.072","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.072","url":null,"abstract":"","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90091784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protective Impacts and Involved Mechanisms of Chlorogenic Acid on Sepsis‑Associated Cognitive Deficits in Rats 绿原酸对脓毒症相关认知缺陷的保护作用及其机制
The Pakistan Veterinary Journal Pub Date : 2023-01-01 DOI: 10.29261/pakvetj/2023.074
{"title":"Protective Impacts and Involved Mechanisms of Chlorogenic Acid on Sepsis‑Associated Cognitive Deficits in Rats","authors":"","doi":"10.29261/pakvetj/2023.074","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.074","url":null,"abstract":"","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"83 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84022430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mitochondrial Dynamics Disturbances in the Liver of Broilers with Pulmonary Hypertension Syndrome 肺动脉高压综合征肉仔鸡肝脏线粒体动力学紊乱
The Pakistan Veterinary Journal Pub Date : 2023-01-01 DOI: 10.29261/pakvetj/2023.073
{"title":"Mitochondrial Dynamics Disturbances in the Liver of Broilers with Pulmonary Hypertension Syndrome","authors":"","doi":"10.29261/pakvetj/2023.073","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.073","url":null,"abstract":"","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89954603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of Enzymatic Hydrolyzed Protein Feeding on Rumen Microbial Population, Blood Metabolites and Performance Parameters of Lactating Dairy Cows 酶解蛋白饲喂对泌乳奶牛瘤胃微生物种群、血液代谢产物及生产性能参数的影响
The Pakistan Veterinary Journal Pub Date : 2023-01-01 DOI: 10.29261/pakvetj/2023.081
{"title":"Impact of Enzymatic Hydrolyzed Protein Feeding on Rumen Microbial Population, Blood Metabolites and Performance Parameters of Lactating Dairy Cows","authors":"","doi":"10.29261/pakvetj/2023.081","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.081","url":null,"abstract":"","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"216 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76593553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Annotating Susceptibility Potential of Single, Double, Tri and Tetra Mixed Infection Bacteria against Non-beta Lactam Antibiotics 单、双、三、四种混合感染细菌对非内酰胺类抗生素的敏感性潜势分析
The Pakistan Veterinary Journal Pub Date : 2023-01-01 DOI: 10.29261/pakvetj/2023.060
{"title":"Annotating Susceptibility Potential of Single, Double, Tri and Tetra Mixed Infection Bacteria against Non-beta Lactam Antibiotics","authors":"","doi":"10.29261/pakvetj/2023.060","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.060","url":null,"abstract":"","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80147598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inulin Effect on Intestinal Mucus-secreting Cells 菊粉对肠道粘液分泌细胞的影响
The Pakistan Veterinary Journal Pub Date : 2023-01-01 DOI: 10.29261/pakvetj/2023.064
{"title":"Inulin Effect on Intestinal Mucus-secreting Cells","authors":"","doi":"10.29261/pakvetj/2023.064","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.064","url":null,"abstract":"","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85703221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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