The Pakistan Veterinary Journal最新文献

Fucoidan Alleviates Intestine Damage in Mice Induced by LPS via Regulation of Microbiota 褐藻糖胶通过调节微生物群减轻 LPS 诱导的小鼠肠道损伤

The Pakistan Veterinary Journal Pub Date : 2024-07-01 DOI: 10.29261/pakvetj/2024.190

引用次数: 0

Designing an Epitope-Based Vaccine against Bovine Viral Diarrhea using Immuno-informatics 利用免疫信息学设计基于表位的牛病毒性腹泻疫苗

The Pakistan Veterinary Journal Pub Date : 2024-04-22 DOI: 10.29261/pakvetj/2024.162

引用次数: 0

Evaluation of Possible Ameliorative Role of Robinetin to Counteract Polystyrene Microplastics Instigated Renal Toxicity in Rats 评估洋槐黄素对抗击聚苯乙烯微塑料引发的大鼠肾毒性的可能改善作用

The Pakistan Veterinary Journal Pub Date : 2024-04-22 DOI: 10.29261/pakvetj/2024.164

引用次数: 0

Applicability of Butterfly Pea Flower Extract as an Alternative Natural Dye in Histopathological Canine Mast Cell Tumor Diagnosis 蝶豆花提取物作为替代天然染料在犬肥大细胞瘤组织病理学诊断中的适用性

The Pakistan Veterinary Journal Pub Date : 2024-04-22 DOI: 10.29261/pakvetj/2024.163

引用次数: 0

Protective Efficacy of Fresh and Aged Macerated Garlic Oils in Safflower Oil Against Intra-Abdominal Adhesions in Rats 鲜榨和陈化红花油中大蒜油对大鼠腹内粘连的保护作用

The Pakistan Veterinary Journal Pub Date : 2023-04-01 DOI: 10.29261/pakvetj/2023.030

G. U. Caliskan, Nuray Emin

{"title":"Protective Efficacy of Fresh and Aged Macerated Garlic Oils in Safflower Oil Against Intra-Abdominal Adhesions in Rats","authors":"G. U. Caliskan, Nuray Emin","doi":"10.29261/pakvetj/2023.030","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.030","url":null,"abstract":"This study aims to investigate the protective efficacy of fresh (FG) and aged (AG) Taşköprü Garlic (Allium sativum L.) macerated oils prepared in safflower oil at different concentrations against post-operative (PO) intra-abdominal adhesions (PIA). No commercial garlic product was used in the study. AG was produced from Taşköprü Garlic, which is one of the geographically indicated products of the European Union. Then FG and AG were kept at two different concentrations (1.5 g/mL & 2.0 g/mL) in safflower (Carthamus tinctorius L.) oil to get macerated oils of FG15, FG20, AG15 and AG20. After spectrophotometric and chromatographic analyzes of the oils, in vivo experiments were made on rats. A cecal abrasion model was applied to six study groups viz FG15, FG20, AG15, AG20, serum physiologic control and oil control. Their preventive activities against PIA were examined macroscopically and histopathologically at different time points (7th‑14th‑21st days PO). The safflower oil did not give successful results in preventing PIA when used alone. The AG macerated oils, especially AG20, caused negative effects on the injured tissue/organ surfaces due to their high ratio of 2,4‑decadienal content. The macerated oils prepared with fresh Taşköprü Garlic in safflower oil (FG15 and FG20) at the rate of 0.1 mL were found successful agents to prevent PIA by intra-abdominal route without any irritant effect in rats. These oils are considered as low cost clinical/laboratory preparations and can be used in laparotomy/laparoscopy to prevent PIA","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"131 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73292307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection and Phylogenetic Analysis of the Tegument Protein Gene of Malignant Catarrhal Fever Virus from Clinical Cases of Cattle and Sheep in the Central Balkan Region 中巴尔干地区牛羊临床病例恶性卡他热病毒被膜蛋白基因检测及系统发育分析

The Pakistan Veterinary Journal Pub Date : 2023-04-01 DOI: 10.29261/pakvetj/2023.032

D. Glišić, ZZ Sapundžić, M. Ninković, B. Milovanovic, O. Stevanović, D. Lausevic, M. Đorđević, B. Kureljušić, V. Milićević

{"title":"Detection and Phylogenetic Analysis of the Tegument Protein Gene of Malignant Catarrhal Fever Virus from Clinical Cases of Cattle and Sheep in the Central Balkan Region","authors":"D. Glišić, ZZ Sapundžić, M. Ninković, B. Milovanovic, O. Stevanović, D. Lausevic, M. Đorđević, B. Kureljušić, V. Milićević","doi":"10.29261/pakvetj/2023.032","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.032","url":null,"abstract":"This study aimed to describe the clinical course of SA-MCF and the duration of viremia in surviving cattle and to perform the phylogenetic analysis of the tegument protein gene of OvHV-2 in cattle in the Central Balkan. A farm housing two heifers and 7 sheep with a confirmed SA-MCF case were selected for the investigation. For the estimation of the length of viremia and the virus shedding, the animals were sampled repeatedly, weekly for two months. For the phylogenetic analysis, a retrospective study was performed on 21 samples from cattle, and 7 samples from sheep, from the Central Balkan. In the blood samples of the survived heifer, the OvHV-2 genome was detected until week 7, in corneal swabs, the OvHV-2 genome was detected until week 6, and in nasal swabs until week two of the study. A retrospective study revealed that out of 21 tested cattle, OvHV–2 was detected in 15 (71.4%), and out of 7 tested sheep, three (42.9%) were positive. The sequenced samples show the highest percentage of similarity with the strains from Brasil KJ658293.1 (100%) and Germany HM216475.1 (100%).. Since there is a variety of different clinical signs similar to other notifiable diseases such as BVD, IBR, and FMD, there is a clear benefit in including SA-MCF in the differential diagnosis in cattle. As mixed farming in the Central Balkan is practiced, implementing SA-MCF monitoring in passive surveillance would allow a better understanding of the disease, ascertaining its prevalence and could provide new information regarding SA-MCF epidemiology","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88619015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hyperbaric Oxygen Therapy, a Possible New Approach in the Treatment of Retinal Damage in Methanol Poisoning in Rats 高压氧治疗是治疗甲醇中毒大鼠视网膜损伤的新途径

The Pakistan Veterinary Journal Pub Date : 2023-04-01 DOI: 10.29261/pakvetj/2023.021

M. Baktır

{"title":"Hyperbaric Oxygen Therapy, a Possible New Approach in the Treatment of Retinal Damage in Methanol Poisoning in Rats","authors":"M. Baktır","doi":"10.29261/pakvetj/2023.021","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.021","url":null,"abstract":"It has been established that peroxidation, inflammatory processes, and organ damage are involved in the pathogenesis of methanol intoxication. Research has shown that using hyperbaric oxygen reduces oxidative stress, inflammation, and tissue damage. In this research, it was investigated how hyperbaric oxygen therapy affected methanol-induced peroxidation and retinal damage in rats. The animals were classified into six categories (n = 8): control group (C), nitrous oxide (N2O) treated group, hyperbaric oxygen (HBO) treated group, methanol (MeOH) treated group, N2O+methanol treated group (N2O+MeOH) and N2O+methanol+hyperbaric oxygen (N2O+MeOH+HBO) treated group. Folate deficiency was started with N2O, four hours before the starting dose (4g/kg) of methanol. The rats were given a methanol maintenance dose (2g/kg, i.p) 24h and 48h after the starting dose. After each methanol administration, rats were given HBO (2 ATA) for 1 hour. Methanol intoxication decreased plasma folic acid levels while increasing formate levels and oxidative stress index and it caused a decrease in the amount of the retinal ganglion cells and thinned the total retinal thickness in folate-deficient mice. HBO treatment reduced the oxidative stress index but did not adequately improve retinal healing. Our findings indicate that the HBO treatment used to treat retinal damage in methanol poisoning was ineffective. However, increasing the duration of HBO therapy may be effective in preventing methanol-induced retinal damage","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84807822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Effects of N Acetylcysteine and Vitamin E against Acrylamide-induced Neurotoxicity in Rats N -乙酰半胱氨酸和维生素E对丙烯酰胺所致大鼠神经毒性的保护作用

The Pakistan Veterinary Journal Pub Date : 2023-04-01 DOI: 10.29261/pakvetj/2023.027

M. Aboubakr, A. Elmahdy, Shaimaa Taima, M. Emam, A. Farag, M. Alkafafy, A. Said, A. Soliman

{"title":"Protective Effects of N Acetylcysteine and Vitamin E against Acrylamide-induced Neurotoxicity in Rats","authors":"M. Aboubakr, A. Elmahdy, Shaimaa Taima, M. Emam, A. Farag, M. Alkafafy, A. Said, A. Soliman","doi":"10.29261/pakvetj/2023.027","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.027","url":null,"abstract":"When many widely ingested foods are heated, a toxin called acrylamide (ACR) is created. The aim of our research was to investigate if N acetylcysteine (NAC) and/or vitamin E (Vit E) could provide protection against neurotoxicity induced by ACR. Rats were classified into seven groups of 7 rats; control (saline); NAC (150 mg/kg bw); Vit E (100 mg/kg bw); ACR (20 mg/kg bw orally); ACR+NAC; ACR+Vit E; ACR+NAC+Vit E. Saline, NAC and/or Vit E were administered orally, once daily for 30 days. Acetylcholinesterase (AChE) inhibition, glutathione depletion, and elevated levels of malondialdehyde (MDA), were all observed after ACR intoxication. Furthermore, ACR diminished superoxide dismutase (SOD) and catalase (CAT) activities, as well as inflammatory mediators as interleukins (IL-1, IL-6), and tumor necrosis factor-α (TNF-α). The combined treatment of ACR with NAC or Vit E dramatically reduced both oxidative and biochemical consequences, with a more frequent return to normal values. To conclude, NAC or Vit E supplementation may alleviate ACR-induced neuronal injury, most likely due to NAC or Vit E antioxidant, anti-inflammatory, and anti-apoptotic effects","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74452980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Alterations of the Oestrous Cycle and Increase in the Blood Pressure in Normotensive Female Rats by Continuous Exposure to Light 持续光照对正常血压雌性大鼠发情周期和血压升高的影响

The Pakistan Veterinary Journal Pub Date : 2023-04-01 DOI: 10.29261/pakvetj/2023.040

Javier Palacios

{"title":"Alterations of the Oestrous Cycle and Increase in the Blood Pressure in Normotensive Female Rats by Continuous Exposure to Light","authors":"Javier Palacios","doi":"10.29261/pakvetj/2023.040","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.040","url":null,"abstract":"The study aims to investigate changes on some cardiac parameters of normotensive female rats due to continual exposure to light. Three months old female Wistar rats were subjected to varying light cycles and intensities, following which cardiovascular parameters were recorded continuously at days 0, 10, 15, 20, 25 and 30. We calculated and recorded the mean arterial pressure, pulse pressure, peripheral resistance and cardiac output. It was observed that continuous exposure to light for 10 days significantly increased the mean arterial pressure (MAP; 103±1 mmHg control vs. 117±6 mmHg at 20 min; P<0.05) and the systolic blood pressure (SBP; 111±1 mmHg control vs. 130±5 mmHg at 20 min; P<0.05). Heart rate (HR) did not significantly change during the exposure to light. However, the pulse pressure (PP) increased at day 20 on exposure to light (18±1 mmHg control vs. 32±4 mmHg at 20 min; P<0.05). It also reduced the peripheral resistance, while increasing the cardiac output in the female rats. It also elevated oestrogen plasma levels and LF/HF ratio, which is a spectral component of ECG denoting heart rate variability (HRV). Continuous exposure to light may induce stress in female rats, leading to hormonal blood pressure, and sympathovagal imbalances","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90663869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Tectochrysin Attenuates Cisplatin-induced Hepatotoxicity by Restoring Biochemical, Inflammatory and Histological Profile in Rats 通过恢复大鼠的生化、炎症和组织学特征来减轻顺铂诱导的肝毒性

The Pakistan Veterinary Journal Pub Date : 2023-04-01 DOI: 10.29261/pakvetj/2023.029

M. Ijaz

{"title":"Tectochrysin Attenuates Cisplatin-induced Hepatotoxicity by Restoring Biochemical, Inflammatory and Histological Profile in Rats","authors":"M. Ijaz","doi":"10.29261/pakvetj/2023.029","DOIUrl":"https://doi.org/10.29261/pakvetj/2023.029","url":null,"abstract":"Cisplatin is an efficacious anticancerous chemotherapeutic agent that is used to cure multiple types of malignancies. However, it has several hazardous effects on multiple organs, particularly liver. Tectochrysin is a naturally occurring flavonoid with extensive pharmacological properties. In this research, the potential anti-oxidant properties of tectochrysin against cisplatin-triggered oxidative stress in rats’ hepatic tissues were investigated. 48 male albino rats were separated into 4 groups: control cisplatin (10 mg/kg), cisplatin + tectochrysin (10 mg/kg + 5 mg/kg), and tectochrysin (5 mg/kg). The trial executed for one month. The biochemical, inflammatory, histopathological and liver markers were evaluated. The results of the research suggested that cisplatin treatment remarkably lowered the activities of glutathione (GSH), superoxide dismutase (SOD), glutathione reductase (GSR), glutathione S-transferase (GST), glutathione peroxidase (GPx) as well as catalase (CAT) while escalated malondialdehyde (MDA) and reactive oxygen species (ROS) levels. Cisplatin administered rats exhibited significantly higher aspartate aminotransferase (AST), alanine aminotransferase (ALT) as well as alkaline phosphatase (ALP) levels. Furthermore, cisplatin administration significantly elevated the inflammatory indicators i.e., interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) as well as nuclear factor kappa-B (NF- κB) and cyclooxygenase-2 (COX-2) activity along with histopathological impairments. Conversely, co-administration with tectochrysin effectively reversed the cisplatin-triggered impairments and abnormalities in the hepatic tissue of rats. The current investigation demonstrated that tectochrysin lowered cisplatin-induced hepatotoxicity owing to its antioxidant, reactive oxygen species scavenging activities and anti-inflammatory effects","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"123 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75798605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1