The Pakistan Veterinary Journal最新文献_第10页

Pathophysiological and Histopathological Ailments in Asphyxial Cardiac Arrest Induced Ischemic Renal Injury 窒息性心脏骤停致缺血性肾损伤的病理生理和组织病理学疾病

The Pakistan Veterinary Journal Pub Date : 2021-03-01 DOI: 10.29261/PAKVETJ/2020.080

Jeong-Hwi Cho

{"title":"Pathophysiological and Histopathological Ailments in Asphyxial Cardiac Arrest Induced Ischemic Renal Injury","authors":"Jeong-Hwi Cho","doi":"10.29261/PAKVETJ/2020.080","DOIUrl":"https://doi.org/10.29261/PAKVETJ/2020.080","url":null,"abstract":"Cardiac arrest (CA) is a sudden interruption in the effective blood flow due to heart failure. The current research aimed to conduct the pathophysiological and histopathological analysis in the kidney in asphyxial cardiac arrest rat model. Cardiac arrest was induced by intravenous injection of vecuronium bromide (2 mg/kg), following stop of mechanical ventilation. Rats were kept on the CA condition for 5 minutes. After that, cardiopulmonary resuscitation (CPR) was done to achieve return of spontaneous circulation (ROSC) following intravenous injection of epinephrine bolus (0.005 mg/kg), sodium bicarbonate (1 mEq/kg) and turn on mechanical ventilation. Then Rats were sacrificed after cardiopulmonary resuscitation (CPR) following asphyxial CA at 6 hrs, 12 hrs, 1 day, 2 days, and 5 days. The intensity of renal injury measured by the serum levels of blood urea nitrogen (BUN), creatinine (Crtn). Moreover, Hematoxylin & eosin, and Periodic Acid Schiff staining in the kidney was done for evaluating the renal histopathological changes. Furthermore, COX-2 immunoreactivity and western analysis were performed in the kidney. Survival rate declined following ROSC compared to the sham group, it showed 80% at 6 hrs and decreased time-dependently to 8% at 5 days. In this study, serum BUN and Crtn levels and renal histopathological scores significantly increased after ROSC in CA. Moreover, COX-2 expression also increased after ROSC in comparison to the sham group with its peak level at 5 days following CA. Renal histological damage score and COX-2 expression were upregulated after ROSC following CA. These results direct that COX-2 takes part in the asphyxial CA-induced ischemic renal injury","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83343849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Antibacterial Activity of Medicinal Flowers against Multi Drug Resistant E. coli 药用花卉对多重耐药大肠杆菌的抑菌活性研究

The Pakistan Veterinary Journal Pub Date : 2021-03-01 DOI: 10.29261/PAKVETJ/2021.019

Iqra Nazir

引用次数: 3

Treatment of Bovine Skin Papillomatosis with Gasoline 汽油治疗牛皮肤乳头状瘤病

The Pakistan Veterinary Journal Pub Date : 2021-03-01 DOI: 10.29261/PAKVETJ/2020.069

Ertan Doğan

引用次数: 0

First Isolation and Genetic Characterization of Bovine Herpesvirus 1 From Cattle in Pakistan 巴基斯坦牛疱疹病毒1型的首次分离及遗传特性分析

The Pakistan Veterinary Journal Pub Date : 2021-03-01 DOI: 10.29261/PAKVETJ/2020.092

H. Rehman

引用次数: 1

Detection of Colistin Resistance in Mannheimia haemolytica & Pasteurella multocida Isolates from Ruminants in Morocco 摩洛哥反刍动物溶血性曼海姆病和多杀性巴氏杆菌分离株粘菌素耐药性检测

The Pakistan Veterinary Journal Pub Date : 2021-03-01 DOI: 10.29261/PAKVETJ/2020.077

G. Sebbar

{"title":"Detection of Colistin Resistance in Mannheimia haemolytica & Pasteurella multocida Isolates from Ruminants in Morocco","authors":"G. Sebbar","doi":"10.29261/PAKVETJ/2020.077","DOIUrl":"https://doi.org/10.29261/PAKVETJ/2020.077","url":null,"abstract":"Colistin is an important drug of last resort against lethal infections with multidrugresistant gram-negative bacteria, this antibiotic interacts on phosphate groups of lipopolysaccharides present on the outer membrane of gram-negative bacteria. This first mechanism of transferable colistin resistance involves a gene called mcr-1. Here, we described different tools such as Colistin susceptibility test, Minimal Inhibitory Concentration to find the colistin profiles of Mannheimia haemolytica and Pasteurella multocida serogroup A Moroccan strain isolated from nasal swabs and lung taken from sheep, goat and cattle with respiratory diseases during January 2015 to December 2017 in six different regions, in addition, we investigated either real time PCR to detect mcr-1 gene. Antimicrobial sensitivity test, was achieved for Fortyone isolates, the resistance rates of isolates from Pasteurellaceae species were between 59 and 71%, which respectively correspond to Mannheimia haemolytica and Pasteurella multocida segroup A. The sensitive ratios were between 29 and 41% which respectively correspond to Pasteurella multocida segroup A and Mannheimia haemolytica. Also, the MIC test was done against colistin, the results showed a resistant profile with a MIC >=64 μg/ml except for 3 strains from ruminants which have a value <2 μg/ml. The real time PCR screening test was detected with ct values ranging from 23 to 31 and confirmed the results obtained by MIC test. To our knowledge the present study is the first study which reports the resistance to colistin in Pasteurellaceae species strains isolated from ruminants in Morocco, our results suggest the necessity and the urgency for establishing a national program for monitoring antibacterial resistance against colistin","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78491769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Bioinformatics Analysis of JAZF1 Gene in Broilers with Ascites Syndrome 肉鸡腹水综合征JAZF1基因的生物信息学分析

The Pakistan Veterinary Journal Pub Date : 2021-03-01 DOI: 10.29261/PAKVETJ/2020.072

F. Guo

{"title":"Bioinformatics Analysis of JAZF1 Gene in Broilers with Ascites Syndrome","authors":"F. Guo","doi":"10.29261/PAKVETJ/2020.072","DOIUrl":"https://doi.org/10.29261/PAKVETJ/2020.072","url":null,"abstract":"Pulmonary vascular remodeling (PVR) is the main characteristic lesion of ascites syndrome (AS) in broilers. JAZF1 plays an important role in PVR, but there is no study on its protein function and structure. In this study, the physical and chemical properties, hydrophilicity/hydrophobicity and transmembrane domain, phosphorylation site and glycosylation site, subcellular localization and signal peptide, secondary and tertiary structure, antigen peptide and conserved domain and phylogenetic relationship of JAZF1 protein were predicted online by bioinformatics tools. The results showed that the number of amino acids of JAZF1 was 243aa, the theoretical isoelectric point was 8.63, the instability index was 58.1, and the average coefficient of hydrophilicity was -0.674. It was found to be a hydrophilic protein having 35 phosphorylation sites and a N-glycosylation site with no transmembrane domain. The protein is expressed in the nucleus, there is no signal peptide distribution in the whole sequence and the secondary structure is mainly composed of random coil and α- helix. There were 7 B cell epitopes, 7 conserved domains and compared with other birds, JAZF1 is 95.61% similar. In summary, from the analysis we came to conclude that the amino acid sequence 64-80aa, 91-108aa, 136-151aa and 179-187aa can be selected as antigen sites and among which 136-151aa may be the best. This study lays a good foundation for follow-up experiments, which then provides powerful conditions for pathological detection of pulmonary vascular remodeling and gene drug therapy of ascites syndrome in broilers.","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"87 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86864591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Hepatoprotective Activity of Nigella sativa and Piper nigrum against Concanavalin A-Induced Acute Liver Injury in Mouse Model 黑草和黑椒对豆豆蛋白a致小鼠急性肝损伤的保护作用

The Pakistan Veterinary Journal Pub Date : 2021-03-01 DOI: 10.29261/PAKVETJ/2020.076

Aqsa Mushtaq

{"title":"Hepatoprotective Activity of Nigella sativa and Piper nigrum against Concanavalin A-Induced Acute Liver Injury in Mouse Model","authors":"Aqsa Mushtaq","doi":"10.29261/PAKVETJ/2020.076","DOIUrl":"https://doi.org/10.29261/PAKVETJ/2020.076","url":null,"abstract":"Nigella sativa and Piper nigrum are implicated in the treatment of various disorders, especially in the management of metabolic, infectious and hepatorenal ailments. However, data on the mechanism behind therapeutic potential of N. sativa and P. nigrum in liver diseases is scarce. The present study investigated the hepatoprotective potential of 70% methanolic extract of N. sativa (NSE) and P. nigrum (PNE) at varying dose levels (100 to 400 mg/kg body weight) against concanavalin A (conA)-induced liver injury. Qualitative phytochemical analysis of plant extracts was performed. Acute hepatic injury was induced by administering intraperitoneally 12mg/kg conA in Balb/c mice. The extent of hepatic injury was measured by analysing serum biochemical parameters, liver antioxidant stress assay and histopathology. Data were analysed statistically. NSE and PNE showed dosedependent hepatoprotective efficacy by lowering the conA-dependent rise in liver transaminase level. Treatment with NSE (400 mg/Kg) and PNE (400 mg/Kg) ameliorated conA-induced alterations in serum oxidative stress markers, biochemical parameters, liver function markers and histopathology. NSE indicated greater effectiveness to ameliorate the acute hepatic injury in comparison with PNE at the same dose. Collectively, pretreatment with NSE and PNE attenuated the liver injury induced by conA, might be through alleviating the antioxidant capacity of experimental mice","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79287854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Role of Oxidative Stress and Antioxidants in Thiram-induced Tibial Dyschondroplasia 氧化应激和抗氧化剂在thiram诱导的胫骨软骨发育不良中的作用

The Pakistan Veterinary Journal Pub Date : 2021-03-01 DOI: 10.29261/PAKVETJ/2020.094

Bingxian Liu

{"title":"Role of Oxidative Stress and Antioxidants in Thiram-induced Tibial Dyschondroplasia","authors":"Bingxian Liu","doi":"10.29261/PAKVETJ/2020.094","DOIUrl":"https://doi.org/10.29261/PAKVETJ/2020.094","url":null,"abstract":"Tetramethyl thiuram disulfide (thiram) is an important dithiocarbamate bactericide; it has been widely used for the control of various diseases in fruits, vegetables, seeds and food grains. However, it also causes environmental pollution problems and poses a threat to human health to a certain extent. Thiram induces tibial dyschondroplasia (TD) by causing oxidative stress and antioxidants imbalance in tibial growth plate in poultry. TD is a skeletal abnormality in fastgrowing poultry birds. It has been considered an economically important disease in poultry that affects poultry industry by carcass loss at meat processing plant due to decrease in disease resistance, production performance, and carcass quality and induces breast cysts and osteomyelitis worldwide. Oxidative stress is developed due to the imbalance of free radical oxygen, which disrupts the equilibrium state of oxidant and antioxidant tending to oxidation. This review is based on the current research, mainly to explore the relationship and mechanism between tibial dyschondroplasia and oxidative stress induced by thiram, which provides a new theoretical foundation for the further research of the related mechanism.","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75047843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Hyaluronic Acid Filler Injection as an Alternative to Surgery for the Correction of Canine Entropion 透明质酸填充注射作为手术矫正犬内翻的替代方法

The Pakistan Veterinary Journal Pub Date : 2021-03-01 DOI: 10.29261/PAKVETJ/2021.001

Ha-Eun Lee

引用次数: 2

Prevalence and Associated Risk Factors of Avian Influenza H9 in Backyard Poultry Populations of Two Agroecological Zones of Pakistan 巴基斯坦两个农业生态区后院家禽H9禽流感流行及相关危险因素分析

The Pakistan Veterinary Journal Pub Date : 2021-03-01 DOI: 10.29261/PAKVETJ/2020.085

M. Hasni

{"title":"Prevalence and Associated Risk Factors of Avian Influenza H9 in Backyard Poultry Populations of Two Agroecological Zones of Pakistan","authors":"M. Hasni","doi":"10.29261/PAKVETJ/2020.085","DOIUrl":"https://doi.org/10.29261/PAKVETJ/2020.085","url":null,"abstract":"Avian influenza subtype H9 is epizootic in backyard poultry population of Pakistan. A cross-sectional study was conducted in two districts of Pakistan from two different agro-ecological zones selected as strata to estimate H9 seroprevalence, virus prevalence and to identify risk factors associated with H9 seroprevalence in backyard poultry. A stratified two-stage cluster sampling method was applied to collect samples (n=420) from two strata (agroecological zone). A total of 210 birds were selected as elementary units from 30 clusters (7x30) in each district from each stratum. Blood and oropharyngeal swab samples were collected from each bird. Sera samples were tested by Hemagglutination Inhibition Test (HI) to detect anti-H9 antibodies and swabs samples were tested by RT-PCR for H9. Overall seroprevalence of H9 in two strata was 57.88% (95% CI 34.88-80.87%), while virus prevalence was calculated to be 3.33%. Four factors were identified to be significantly associated (P<0.05) with H9 seroprevalence in the multivariable logistic regression analysis. The odds for H9 seropositivity were 9.43 times higher in flocks with fighting cockerel compared to those having no fighting cockerel (95% CI 4.68 -18.96). Existence of any pond, canal or any other water body near home premises also enhanced likelihood of H9 seroprevalence (OR: 10.04; 95% CI 3.27- 30.83). Backyard chicken raised with other bird species (like ducks, pigeons, or captive wild birds) had higher chances of H9 seropositivity than chicken raised alone (OR 4.12, 95% CI 1.35-12.56). Visit of any farm vehicle to the village had odds ratio of 20.96 (95% CI 5.74-76.51). Future surveillance is recommended to check the level of disease throughout the country","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75980873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4