Hepatoprotective Activity of Nigella sativa and Piper nigrum against Concanavalin A-Induced Acute Liver Injury in Mouse Model

The Pakistan Veterinary Journal Pub Date : 2021-03-01 DOI:10.29261/PAKVETJ/2020.076

Aqsa Mushtaq

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引用次数: 8

Abstract

Nigella sativa and Piper nigrum are implicated in the treatment of various disorders, especially in the management of metabolic, infectious and hepatorenal ailments. However, data on the mechanism behind therapeutic potential of N. sativa and P. nigrum in liver diseases is scarce. The present study investigated the hepatoprotective potential of 70% methanolic extract of N. sativa (NSE) and P. nigrum (PNE) at varying dose levels (100 to 400 mg/kg body weight) against concanavalin A (conA)-induced liver injury. Qualitative phytochemical analysis of plant extracts was performed. Acute hepatic injury was induced by administering intraperitoneally 12mg/kg conA in Balb/c mice. The extent of hepatic injury was measured by analysing serum biochemical parameters, liver antioxidant stress assay and histopathology. Data were analysed statistically. NSE and PNE showed dosedependent hepatoprotective efficacy by lowering the conA-dependent rise in liver transaminase level. Treatment with NSE (400 mg/Kg) and PNE (400 mg/Kg) ameliorated conA-induced alterations in serum oxidative stress markers, biochemical parameters, liver function markers and histopathology. NSE indicated greater effectiveness to ameliorate the acute hepatic injury in comparison with PNE at the same dose. Collectively, pretreatment with NSE and PNE attenuated the liver injury induced by conA, might be through alleviating the antioxidant capacity of experimental mice

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黑草和黑椒对豆豆蛋白a致小鼠急性肝损伤的保护作用

黑草和黑椒涉及到各种疾病的治疗，特别是在代谢，感染性和肝肾疾病的管理。然而，关于藜麦和黑孢霉在肝脏疾病治疗潜力背后的机制的数据很少。本研究研究了不同剂量水平(100 ~ 400 mg/kg体重)的藜麦70%甲醇提取物(NSE)和藜麦70%甲醇提取物(PNE)对豆蛋白A (conA)诱导的肝损伤的保护作用。对植物提取物进行了定性植物化学分析。腹腔注射12mg/kg conA诱导Balb/c小鼠急性肝损伤。采用血清生化指标分析、肝脏抗氧化应激试验及组织病理学检测肝损伤程度。对数据进行统计学分析。NSE和PNE通过降低cona依赖性肝转氨酶水平的升高，显示出剂量依赖性的肝保护作用。NSE (400 mg/Kg)和PNE (400 mg/Kg)治疗可改善cona诱导的血清氧化应激标志物、生化参数、肝功能标志物和组织病理学变化。在相同剂量下，NSE对急性肝损伤的改善效果优于PNE。综上所述，NSE和PNE预处理可能通过降低实验小鼠的抗氧化能力来减轻conA所致的肝损伤

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The Pakistan Veterinary Journal

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